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1.
一氧化氮在成兔与胚胎兔创面愈合过程中含量的比较   总被引:2,自引:1,他引:1  
目的;分析胚胎无瘢痕愈合的潜在原因,研究NO(一氧化氮)在成人型和胚胎型愈合过程中的差别。方法:在已瓣胎兔创伤模型的基础上。用一氧化氮酶法试剂盒检测胚胎兔和成兔皮肤匀浆液中NO的含量,并对结果进行比较。  相似文献   

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目的 :分析胚胎无瘢痕愈合的潜在原因 ,研究NO(一氧化氮 )在成人型和胚胎型愈合过程中的差别。方法 :在已建立的胎兔创伤模型的基础上 ,用一氧化氮酶法试剂盒检测胚胎兔和成兔皮肤匀浆液中NO的含量 ,并对结果进行比较。结果 :①正常胎兔不同孕期皮肤中NO含量无差别。②正常胎兔皮肤中NO含量高于正常成兔皮肤中NO含量 (P <0 .0 1)。③创伤胎兔皮肤中NO含量高于正常胎兔皮肤中NO含量 (P <0 .0 1)。④创伤成兔皮肤中NO含量高于正常成兔皮肤中NO含量 (P <0 .0 1)。⑤创伤胎兔皮肤中NO含量高于创伤成兔皮肤中NO含量 (P <0 .0 1)。结论 :NO参与了胚胎和成年动物的创面愈合过程 ,并在两种愈合过程中存在差别  相似文献   

3.
一氧化氮与创面愈合   总被引:4,自引:0,他引:4  
一氧化氮的产生在创伤后有改变,它参与调节创伤后炎症反应,肉芽组织形成和皮肤生长等创面愈合过程。对其生成的诱导和抑制有望成为调控创面愈合的一个途径。  相似文献   

4.
创面愈合过程中内源性FGF含量变化的研究   总被引:1,自引:0,他引:1  
为探讨创面愈合的机制,本实验免疫组织化学方法,对大鼠断层供应区创面愈合过程中伤后4天,8天,12天和16天创面内源性成纤维细胞生长因子(FibroblastGrowthFactor,FGF)变化进行了研究。结果表明:创伤愈合过程中内源性FGF有规律性变化,以伤后8天时相对含量最多,伤后早期和伤后晚期之,创面愈合后其内源性含量进一步减少,结论:创面愈合过程中,内源性FGF的变化促进创面愈合,是创面愈  相似文献   

5.
为探讨创面愈合的机制,本实验通过免疫组织化学方法,对大鼠断层供皮区创面愈合过程中伤后4天,8天,12天和16天创面内源性成纤维细胞生长因子(FibroblastGrowthFactor.FGF)变化进行了研究。结果表明:创面愈合过程中内源性FGF有规律性变化,以伤后8天时相对含量最多,伤后早期和伤后晚期次之,创面愈合后其内源性含量进一步减少。结论:创面愈合过程中,内源性FGF的变化促进了创面愈合,是创面愈合的机制之一,结合内源性FGF变化,合理外用FGF对促进创面愈合可能会取得更好的效果。  相似文献   

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创伤愈合是一个复杂的生物学过程,创面愈合的过程分为炎症反应、肉芽组织形成、再上皮化及伤口闭合后塑形三个阶段,是一个有序的变化过程.而许多重要的步骤都需要以营养为基础,不同的营养水平将影响代谢和生理过程.  相似文献   

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肿瘤是细胞的修复机制发生障碍,导致新转化的细胞进入无限制生长状态。而机体遭受创伤后组织启动愈合信号,某些基因激活表达,使细胞脱离停滞状态,发生增殖转化。由于机体在进行组织更新和修复的过程中,效应细胞有不停止生长的危险,因而可能造成细胞的致瘤性转化,所以必须启动防治其恶性增殖、侵袭转移的机制,以防止肿瘤的发生犤1,2犦。而少数肿瘤细胞在伤口部位生长、发展的现象似乎也提示人们伤口因素能诱发肿瘤的形成,甚至参与了肿瘤切除术后复发的机制。通过分析肿瘤发生和创面愈合的分子和细胞活动情况及两者间的相互关系与…  相似文献   

8.
胶原与创面愈合   总被引:12,自引:0,他引:12  
胶原的变化贯穿于创面愈合的全过程,对创面的预后起重要作用。近年来的研究对胶原与创面愈合的关系有了新的认识。如何监测、控制创面愈合过程中胶原的代谢,利用胶原的特点促进创面愈合已成为目前创面愈合研究的重要方向。  相似文献   

9.
透明质酸与创面愈合   总被引:3,自引:0,他引:3  
创面愈合是一个复杂的生物学过程,而胶原代谢又贯穿于组织修复的整个过程中,影响胶原代谢的因素很多,实验证明作为细胞外基质重要组成成分的透明质酸,在其中起着重要作用。  相似文献   

10.
创面愈合过程中EGF基因表达变化的研究   总被引:4,自引:0,他引:4  
创面愈合是一个复杂的过程。文献中关于创面愈合过程中,上皮细胞生长因子(epidermalgrowthfactor,EGF)基因表达变化的研究较少,为此设计了本项研究。采用Wistar大鼠,在脊柱两侧制作1.5cm×1.5cm中厚断层皮肤缺损创面4个,于伤后第4,8,12和16天取材,用地高辛标记EGFcDNA探针原位杂交方法,观察创面愈合过程中,EGF基因活化表达的mRNA在组织中的分布变化。发现:在伤口愈合的全过程中,EGF基因均有明显表达,以伤后第8天表达最强。提示:应用某种方法促进EGF基因表达,可能会促进伤口愈合  相似文献   

11.
创伤修复是损伤组织恢复其完好结构的必需过程,大致分为三个阶段:①局部炎症反应阶段:②细胞增殖分化及肉芽组织形成阶段:③组织重建阶段。有研究发现包括一氧化氮(nitric oxide,NO)在内的小分子自由基对伤口的良好愈合起到了关键作用。现就NO在创伤修复过程中的作用作以阐述。  相似文献   

12.
胚胎兔、成兔皮肤在正常发育及创伤愈合过程中IgG的表达   总被引:2,自引:0,他引:2  
目的 分析胚胎兔,成兔胚皮肤在便伤愈合过程中IgG表达的差异,为胎儿型及成人型愈合机制的研究提供依据。方法 在建立胎兔皮肤创伤模型的基础上,采用皮肤均化的方法,提取正常及创伤组织中的免疫球蛋白IgG,用双抗体夹心ELISA法进行含量测定。结果 随正常胎兔(孕23-28天)孕期的延长,皮肤中IgG有合成增加的趋势,而正常成免皮肤的IgG含量显著高于胎兔(P<0.01);胎兔皮肤创伤后不同时间(1-6d)的IgG含量与对应的正常胎兔(孕23-28天)相比,只伤后第1,2天增高(P<0.01),而不同孕期的创伤胎兔之间差异无显著意义。成兔皮肤创伤后从第2天起IgG合成量明显高于正常成兔(P<0.01),并呈现明显的上升趋势,对胎,成兔皮肤创伤后IgG变化趋势的动态发现胎兔呈平坦线性,成兔呈上升线性。结论 在胚胎发育成熟的过程中,免疫系统日臻完善,虽然IgG在较低水平,但在创伤后仍表现出一定的免疫反应,而成兔在创伤后却表现出强烈的免疫反应,不仅含量高而且趋势显著,免疫反应的不同是胎儿愈合与成人型愈合的本质差别之一。  相似文献   

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Wound healing in the fetus proceeds through a series of steps that differ in the fetus and the adult. At each phase of this complex process, there is signaling between the tissue cells and the wound microenvironment, signals that are mediated by and through the extracellular matrix. We postulate that these signals occur earlier in fetal wounds, resulting in more rapid repair. To investigate this, we compared the first 24 hours of wound healing in the rabbit fetus and adult, using antibodies against key extracellular matrix macromolecular components: laminin, fibronectin, and type-specific collagens I, III, IV, and V. Fibronectin was the first matrix component to be deposited, and was visualized as early as four hours after fetal wounding and 12 hours after adult wounding. There was no evidence of new laminin or collagen deposition in either the fetal or adult wounds at any time point examined. The early deposition of fibronectin, a matrix adhesion molecule that provides a scaffolding for epithelial migration, may underlie the rapid reepithelialization observed in fetal wounds.  相似文献   

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Recent clinical and experimental evidence suggests that the fetus responds to injury in a fashion fundamentally different from that of the adult. Our initial experience with human open fetal surgery reinforces experimental observations that the fetal wounds heal without the scarring, inflammation, and contraction that often accompany adult wounds. In this study we examine fetal wound fluid in an attempt to elucidate the control mechanisms that endow the fetus with unique healing properties. The extracellular matrix of fetal wounds is rich in hyaluronic acid, a glycosaminoglycan found in high concentrations whenever there is tissue proliferation, regeneration, and repair. We establish that wound fluid from the fetus contains high levels of hyaluronic acid-stimulating activity that may underlie the elevated deposition of hyaluronic acid in the fetal wound matrix. In contrast there was no hyaluronic acid-stimulating activity present in adult wound fluid. Hyaluronic acid, in turn, fosters an extracellular environment permissive for cell motility and proliferation that may account for the unique properties observed in fetal wound healing.  相似文献   

17.
Most D  Efron DT  Shi HP  Tantry US  Barbul A 《Surgery》2002,132(5):866-876
BACKGROUND: Excisional wound healing in inducible nitric oxide synthase knockout (iNOS-KO) mice has been previously shown to be impaired compared with their background strain controls. Incisional wounds were created in this experiment in both types of animals and paradoxically were found to heal with the same rapidity and breaking strength in both groups. METHODS: Dorsal 2.5 cm incisional wounds were created in iNOS-KO mice, as well as their parental strain controls (C57BL/6J). Standardized polyvinyl alcohol sponges were implanted in the wounds to allow for measurement of collagen deposition. Animals were harvested on postoperative days (PODs) 3, 5, 7, 10, 14, and 28, and their wounds subjected to tensiometric breaking strength analysis. Nonisotopic in situ hybridization quantitative analysis for iNOS, endothelial NOS (eNOS), basic fibroblast growth factor (bFGF), transforming growth factor-beta1 (TGF-beta1), vascular endothelial growth factor (VEGF), and interleukin-4 (IL-4) expression in the wounds was performed. Hydroxyproline levels were quantitated in the harvested polyvinyl alcohol sponges. Data were analyzed with the Students t test. RESULTS: No significant differences were found in breaking strengths or levels of hydroxyproline (and thus collagen) in iNOS-KO versus wild-type wounds at all tested time points. Flawed iNOS expression levels in iNOS-KO animals were similar to (functional) iNOS expression in wild-types. eNOS and bFGF expression nearly doubled on POD 7 in iNOS-KO incisions (P =.002, and.002), respectively and remained 200% to 300% elevated thereafter. TGF-beta1 expression was increased approximately 50% to 100% in iNOS-KO wounds on PODs 5 and 7 (P =.006 and.01, respectively). VEGF and IL-4 expression was elevated by 25% to 100% in wild-type compared with iNOS-KO animals at all time points (P <.01). CONCLUSIONS: The overexpression of TGF-beta1 and eNOS may represent mechanisms in iNOS-KO mice to compensate for their loss of functional iNOS, resulting in incisional wound healing equivalent to controls. Their impaired expression of VEGF and IL-4, on the other hand, may partially explain the delayed excisional wound healing noted in these animals.  相似文献   

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