腹腔镜与开腹子宫次全切除术的疗效比较及对卵巢功能的影响

目的探讨腹腔镜子宫次全切除术与开腹子宫次全切除术的疗效比较及对卵巢功能的影响。方法 60例有子宫次全切除术指征的子宫良性病变患者者随机分为腹腔镜组和开腹组各30例,观察比较两组的手术效果及术前术后不同时间两组对卵巢功能近远期的影响。结果腹腔镜组的手术时间、自觉症状缓解时间、伤口愈合时间及术后起床活动时间、术后住院时间均明显短于开腹组(P0.05)。腹腔镜组术后24个月E2、P、INHB较术前比较明显下降,FSH、LH与术前比较均明显升高。开腹组术后6个月、12个月、24个月的E2、P、INHB均较术前明显下降,FSH、LH较术前明显升高。且开腹组术后6个月、12个月E2、P、INHB水平均较腹腔镜组明显下降,而术后6个月、12个月开腹组FSH、LH水平较腹腔镜组明显提高。结论腹腔镜下次全子宫切除术能维持卵巢功能,手术效果明显优于传统开腹组。     

腹腔镜及开腹子宫次全切除术对卵巢功能的影响

目的探讨腹腔镜与开腹子宫次全切除术对卵巢功能近远期功能的影响。方法选择因子宫良性病变保留宫颈而行子宫次全切除患者218例,分为两组:腹腔镜组(122例)和开腹组(96例)。所有患者分别于术前、术后1、3、6、12、24个月,采静脉血测定E2、FSH、LH、P、INHB的水平。结果两组同期比较术后3个月内,E2、P、INHB、FSH、LH水平无差异(P>0.05),术后6～24个月开腹组较腹腔镜组E2、P、INHB水平低,FSH、LH水平高,差异有显著性(P<0.05)。结论腹腔镜下子宫次全切术对卵巢功能的影响小于传统开腹手术,在无腹腔镜手术禁忌证情况下,应作为首选术式。     

腹腔镜卵巢囊肿剔除术对卵巢囊肿患者卵巢功能、血清抗苗勒管激素、抑制素B的影响及其意义

目的:探讨腹腔镜卵巢囊肿剔除术(LOCR)治疗卵巢囊肿对患者卵巢功能、血清抗苗勒管激素(AMH)、抑制素B(INHB)水平的影响.方法:选取郑州长江中医院2015年1月—2017年6月手术治疗的114例卵巢囊肿患者,以患者手术方法为分组方案,将其分为A组(53例)、B组61例,A组采用LOCR手术治疗,B组采用开腹手术治疗;同时选取60例正常妇女测量相关指标作为对照组;对比三组研究对象的卵巢功能、血清AMH、INHB水平、卵泡刺激素(FSH)、雌激素(E2)、促黄体生成素(LH).结果:术后3个月,A组患者的卵巢体积与B组比较差异无统计学意义(P>0.05),A组患者的窦卵泡数目多于B组(P<0.05);术前对A、B组患者的血清AMH、INHB基线水平进行检测,两组无实质性差异(P>0.05);术后3个月复查两组患者的AMH水平,A组高于B组(P<0.05),A组的INHB则低于对照组(P<0.05);术前对两组患者的FSH、E2、LH基线水平进行检测比较,差异无有统计学意义(P>0.05);术后3个月FSH、LH再次复查A组患者则均低于B组(P<0.05),E2水平组间对比,A组则高于B组(P<0.05).结论:LOCR治疗卵巢囊肿较开腹手术更加有利于术后卵巢功能的恢复,避免血清AMH、INHB水平在术后大幅下降.     

补肾活血法对卵巢子宫内膜异位囊肿剔除术后卵巢储备功能的干预作用

【目的】探讨卵巢子宫内膜异位囊肿剔除术对卵巢储备功能的影响及补肾活血中药对术后卵巢储备功能的干预作用。【方法】选择拟行腹腔镜下卵巢子宫内膜异位囊肿剔除术的病例70例,随机分为中药组和对照组各35例,中药组术后给予补肾活血中药口服,对照组术后不予中药治疗。术前、术后第1个月、第4个月观察月经等情况,检测血清基础卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、抑制素B(INHB)水平,评估患者手术前后的卵巢储备功能。【结果】(1)术后第1个月,2组患者月经异常率稍增加,但与术前比较差异无统计学意义(P﹥0.05),且随术后时间延长,月经情况逐渐恢复。(2)术后第1个月2组的LH、FSH水平均升高,E2、INHB水平均下降,与术前比较,差异均有统计学意义(P<0.05)。术后第4个月,中药组LH、FSH水平降低,E2、INHB水平升高(与术后第1个月比较,P<0.05),并基本恢复到术前水平(与术前比较,P>0.05);2组间比较,差异均有统计学意义(P<0.05),表明中药组在改善血清性激素及INHB水平方面优于对照组。【结论】补肾活血中药可降低术后患者的血清基础FSH、LH水平,升高抑制素B、基础E2水平,达到改善卵巢储备功能的目的。     

不同手术方式对子宫肌瘤患者卵巢功能的影响比较

目的:探讨不同手术方式对子宫肌瘤患者卵巢功能的影响。方法:选取84例子宫肌瘤患者,其中行全子宫切除术(保留双侧附件)治疗的40例患者作为对照组,行子宫肌瘤剔除术治疗的44例患者作为观察组,于术前、术后3个月和6个月检测所有患者的卵泡刺激素(FSH)、雌二醇(E2)以及黄体生成素(LH)水平,并分别进行比较分析。结果:观察组术前、术后FSH、E2、LH水平比较差异无统计学意义(P>0.05),但对照组术后FSH、LH水平高于术前,E2水平低于术前,差异均有统计学意义(P<0.05)。术后3、6个月观察组FSH、LH水平低于对照组,E2水平高于对照组,差异均有统计学意义(P<0.05)。结论:子宫肌瘤剔除术较全子宫切除术对患者卵巢功能影响较小,值得在临床中推广应用。     

子宫附件切除对女性生殖激素的影响

目的探讨不同术式的子宫附件切除对女性生殖激素的影响。方法将65例40～45岁月经周期规则的良性妇科肿瘤患者按不同术式分为3组:组Ⅰ(n=22)行单纯次全子宫切除术;组Ⅱ(n=23)行单纯全子宫切除术;组Ⅲ(n=20)行次全子宫切除术加一侧附件切除术。各组于术前1 d及术后1周、3个月、1年分别测定血清卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、催乳素(PRL)的水平,比较各种激素手术前后的变化。结果术后1周,各组E2均较术前1 d明显降低(P<0.05);之后各组E2逐渐上升,术后1年时组Ⅰ、组Ⅱ基本接近术前水平,但组Ⅲ仍较术前显著降低(P<0.05)。术后3个月时各组FSH、LH较术前1 d及术后1周明显升高(P<0.05);术后1年时各组FSH、LH仍较术前1 d显著升高(P<0.05),组Ⅰ、组Ⅱ中FSH、LH较术后3个月时下降(P<0.05)。术后1周,各组PRL均较术前明显升高(P<0.05)。结论不同术式的子宫附件切除术后,近期表现为E2明显下降,PRL显著上升,以后逐渐恢复。FSH、LH术后3个月时明显升高,之后可能因为卵巢通过血管再生及局部内环境的调整,FSH及LH逐渐下降,1年...     

子宫全切术与子宫肌瘤剔除术治疗子宫肌瘤对卵巢功能的影响

目的 探讨子宫全切术和子宫肌瘤剔除术对子宫肌瘤患者卵巢功能的影响.方法 选择妇产科确诊的子宫肌瘤患者138例,69例行子宫全切术(全切组),69例行子宫肌瘤剔除术(剔除组),于术前及术后1、3、6个月测量卵泡刺激素(FSH)、黄体生成素(LH)和雌二醇(E2),随访1年了解围绝经期症状.结果 (1)全切组术后FSH、LH水平高于术前,E2水平低于术前(P＜0.05或P＜0.01),而剔除组仅6个月时的LH水平高于术前;全切组术后3、6个月时的FSH、LH水平高于剔除组,1、3、6个月时的E2水平低于剔除组(P＜0.05或P＜0.01).(2)剔除组1、3、6个月时月经完全恢复正常率分别是4.35％ 、33.33％和91.3％.全切组和剔除组术后1年内围绝经期症状发生率分别为39.13％、10.15％(x2=16.305,P =0.000).结论 子宫肌瘤剔除术具有保留子宫、减少围绝经期症状、维持女性正常卵巢功能等优点,是治疗子宫肌瘤的理想术式.     

子宫切除对卵巢功能及性功能的影响

目的：了解子宫切除及术式对卵巢功能、术后性生活的影响。方法：采用放射免疫测定法对42例年龄34～41岁、月经周期规则、卵巢正常、手术方式不同的子宫肌瘤患者手术前及术后2周、3个月、6个月、12个月性激素(FSH、E2)测定，以及术后是否出现卵巢功能衰退、性功能下降等症状进行评估。结果：术后2周子宫全切组及次全切组患者E2下降，FSH升高，与术前相比差异有显著性(P＜0．01或＜0．05)；术后3～12个月E3、FSH与术前相比无显著性差异(P＞0．05)；手术前后全切组与次全切组相比，E2、FSH变化均无显著性差异；术后有8例性满意度下降，与E2变化无关。结论：子宫切除会引起卵巢功能暂时性下降，可于术后逐渐恢复，与手术方式无明显关系；术后性满意度下降与性激素变化无关。     

子宫切除术后卵巢功能变化的临床观察

目的了解子宫切除术后保留卵巢功能的变化,探讨子宫切除对卵巢功能的影响。方法选择因子宫良性病变行子宫切除术的病例106例,进行1年的追踪观察,利用放免方法分别测定术前、术后3个月、6个月、1年的性激素水平。结果子宫切除术后6个月,观察组E、P值显著低于术前及对照组,FSH、LH值显著高于术前及对照组(P<0.05)。结论子宫切除后女性卵巢功能有所下降,应慎重对待子宫切除。     

不同子宫术式对受术者性激素水平影响的探讨

目的:观察子宫和(或)单侧附件切除手术后对受术者性激素的影响及其恢复途径.方法:120例良性妇科肿瘤患者按不同术式分为4组:Ⅰ组行单纯筋膜内子宫全切术,Ⅱ组行单纯全子宫切除术,Ⅲ组行全子宫切除术加一侧附件切除术,Ⅳ组行单侧附件切除术.各组于术前、术后1周、3个月、1年后测定血清性激素水平,比较手术前、后FSH、LH、E2、P、T、PRL的变化.结果:术后1周各组E2水平均降低,PRL水平上升,手术前、后比较差异有显著性(P<0.01),FSH、LH、P、T未见明显差异.术后3月,各组E2水平均高于术后1周,差异有显著性(P<0.01,P<0.05),各组PRL水平较术后1周下降,差异也有显著性(P<0.01,P<0.05),Ⅰ组、Ⅱ组、Ⅲ组水平均较术后1周上升,差异有显著性(P<0.01,P<0.05),Ⅳ组未见明显升高,差异无显著性(P>0.05).手术1年后,各组E2水平继续上升,接近或达到术前水平,与术前差异无显著性(P>0.05),Ⅰ组、Ⅱ组、Ⅲ组FSH水平继续上升,高于术前水平,差异有显著性(P<0.05),Ⅳ组术后3个月就已下降至术前水平,与术前差异无显著性(P>0.05).结论:子宫和(或)单侧附件切除术后,近期对内分泌功能有不同程度的影响,以E2、PRL最敏感,E2水平明显下降,PRL显著上升,以后逐渐恢复.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.