益气补肾汤提高慢性阻塞性肺病患者体液免疫功能的研究

目的 比较益气补肾汤治疗慢性阻塞性肺病(COPD)患者前后血清免疫球蛋白和补体水平,了解患者体液免疫水平变化情况.方法 观察COPD稳定期患者益气补肾汤治疗前后的自身对照,并设对照组.主要监测以下指标:血清IgG、IgM、IgA、补体C3、补体C4.结果 益气补肾汤能显著提高患者的血清IgG、IgM、补体C3水平(P<0.01).结论 益气补肾汤能显著提高COPD稳定期患者机体免疫力.     

益气养精法治疗中晚期肺癌的探讨

探讨益气养精法治疗中晚期肺癌的理论依据与临床应用。临床观察发现益气养精法治疗中晚期肺癌具有改善症状、延长患生存期、提高生存质量等作用。实验研究证实益气养精法治疗恶性肿瘤的作用是：抑制肿瘤的发生和发展；调节患机体的免疫功能；改善骨髓造血功能和物质代谢。     

中医益气法治疗肺癌探析

中医治疗肺癌在于减轻手术、放化疗的副作用,提高机体免疫力,抑制肿瘤细胞转移复发,缓解临床症状,改善病者体质,延长生命,提高生存质量。气虚是肺癌发生的基本病机,临床常用益气养阴、益气化痰、益气活血三法从气论治肺癌。各项临床研究及实验研究也证实了益气法治疗肺癌的科学性。     

中医益气法治疗肺癌探析简

中医治疗肺癌在于减轻手术、放化疗的副作用,提高机体免疫力,抑制肿瘤细胞转移复发,缓解,临床症状,改善病者体质,延长生命,提高生存质量。气虚是肺癌发生的基本病机,临床常用益气养阴、益气化痰、益气活血三法从气论治肺癌。各项临床研究及实验研究也证实了益气法治疗肺癌的科学性。     

益气护卫汤在哮喘缓解期的临床运用

目的总结益气护卫汤在哮喘缓解期的临床运用。方法通过收集整理益气护卫汤治疗哮喘缓解期的机理、组方理论及临床有效病案来论述益气护卫汤与哮喘缓解期两者之间的相关性。结果痰瘀伏肺为哮喘反复发作的夙根,而哮喘反复发作的夙根形成责之于肺、脾、肾的气阳虚,通过补肺、脾、肾之气阳来解决哮喘患者的宿根问题。结论气阳虚弱是哮喘发作的内因,基于"气阳虚弱"而创制的益气护卫汤可改善哮喘患者体质,提高机体抵御功能。     

益气方药对肿瘤疾病的免疫调节作用研究进展

益气方药所发挥的免疫调节作用可能是其抗肿瘤的重要机制.在固有免疫方面,可能与其调节巨噬细胞及NK细胞等的活性相关;而在特异性免疫方面,益气方药可调动机体潜在的抗肿瘤机能,可能与其可调节免疫细胞亚群有关.无论是肿瘤本身引起的免疫异常,还是化疗药物在治疗中造成的免疫受损,益气方药均可发挥较强的免疫调节作用.     

益气健脾方对化疗后骨髓及免疫功能影响简

目的：探讨益气健脾方对环磷酰胺化疗后骨髓抑制及免疫抑制的影响。方法：灌胃第11天：眼眶取血,进行血细胞分析;采用称重法测定胸腺、脾脏重量并计算脏器指数;腹腔巨噬细胞吞噬功能的测定;NK细胞活性检测。结果：益气健脾方显著提高化疗小鼠胸腺指数、腹腔巨噬细胞吞噬功能和NK细胞活性。结论：益气健脾方具有促进骨髓细胞生长,提高机体免疫功能,抵御化疗所引起的骨髓抑制及免疫功能下降的作用。     

益气健脾化积方对胃癌患者免疫功能调节作用的理论机制探讨

[目的]探讨益气健脾化积方对胃癌(Gastric Cancer,GC)患者免疫功能调节作用的理论机制。[方法]从中医古籍理论与调节机体免疫功能相类之载述、益气健脾化积方组方意义与扶正抗癌的作用关系以及益气健脾化积方所含药物的药理作用与双向免疫调节的作用关系等角度进行归纳、分析和探讨,阐述益气健脾化积方对胃癌患者免疫调控作用的理论基础。[结果]益气健脾化积方对GC患者免疫调节的作用,是建立在中医理、法、方、药的理论基础之上,且与益气健脾化积方中传统中医功效理论及所含药物具有的双向免疫调节的药理作用相关。[结论]益气健脾化积方具有的益气健脾、扶正抗癌中医功效和所具有的双向免疫调节作用,是对胃癌患者发挥临床疗效作用的理论机制之一。     

益气健脾方对化疗后骨髓及免疫功能影响

目的 :探讨益气健脾方对环磷酰胺化疗后骨髓抑制及免疫抑制的影响。方法 :灌胃第11天:眼眶取血,进行血细胞分析;采用称重法测定胸腺、脾脏重量并计算脏器指数;腹腔巨噬细胞吞噬功能的测定;NK细胞活性检测。结果 :益气健脾方显著提高化疗小鼠胸腺指数、腹腔巨噬细胞吞噬功能和NK细胞活性。结论 :益气健脾方具有促进骨髓细胞生长,提高机体免疫功能,抵御化疗所引起的骨髓抑制及免疫功能下降的作用。     

从脾论治角膜溃疡40例观察

从脾论治角膜溃疡体现了祖国医学“扶正祛邪”的基本理论,本文对于病毒性角膜溃疡久治不愈,脾胃气虚或过用苦寒药后伤及脾胃者,采用补脾益气,祛湿解毒的法则,治疗总结角膜溃疡40例,临床有效率为97.5％,可能与健脾益气药黄芪等有增强机体免疫功能的作用有关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.