重组nesfatin-1蛋白对ox-LDL诱导小鼠巨噬细胞系RAW264.7泡沫化的抑制作用和机制研究

目的探究nesfatin-1对氧化型低密度脂蛋白(ox-LDL)诱导的巨噬细胞泡沫化的作用和分子机制。方法将小鼠RAW264.7巨噬细胞分成3组:对照组(不进行任何处理)、模型组(加入80 mg/L ox-LDL诱导泡沫细胞形成)、nesfatin-1组(加入80 mg/L ox-LDL前2 h加入1×10~(-8)mol/L重组小鼠nesfatin-1蛋白)。油红O染色观察泡沫细胞的形成;试剂盒检测细胞内胆固醇水平[总胆固醇(TC)、游离胆固醇(FC)、胆固醇酯(CE)和胆固醇酯化率(CER)];Western blot检测nesfatin-1蛋白、脂质摄取相关蛋白(CD36和LOX-1)与胆固醇排出调节蛋白(ABCA1和ABCG1)和AMPK通路蛋白[AMPK和磷酸化AMPK(p-AMPK)]表达。结果与对照组相比,模型组中nesfatin-1蛋白表达显著降低,细胞质中出现大量被染成红色的脂质颗粒,TC、FC、CE和CER水平升高,CD36和LOX-1表达上调,ABCA1、ABCG1和p-AMPK/AMPK水平下调,差异均有统计学意义(P0.05)。与模型组相比,nesfatin-1组中红色的脂质颗粒减少,TC、CE和CER水平降低,LOX-1表达下调,ABCG1和p-AMPK/AMPK水平上调,差异均有统计学意义(P0.05);而CD36和ABCA1表达变化不大,差异无统计学意义(P0.05)。结论在ox-LDL诱导的泡沫细胞中nesfatin-1表达下调,nesfatin-1重组蛋白可抑制ox-LDL诱导的巨噬细胞泡沫化,这极可能是通过激活AMPK通路,进而下调LOX-1表达,上调ABCG1表达发挥作用的。     

葛根素对RAW264.7源性泡沫细胞胆固醇摄取及外排功能的影响

目的:探讨葛根素(puerarin,Pur)对RAW264.7源性泡沫细胞胆固醇摄取及外排功能的影响。方法:以氧化低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)诱导RAW264.7源性泡沫细胞。细胞经处理后,利用荧光探针Di I-ox-LDL检测泡沫细胞摄取ox-LDL的能力,NBD标记胆固醇外排实验检测泡沫细胞的胆固醇外排功能,Western blot法检测LC3II、P62、CD36、ATP结合盒转运蛋白A1(ATP-binding cassette transporter A1,ABCA1)、溶酶体酸性脂肪酶(lysosomal acid lipase,LAL)以及p-AMPK的蛋白水平。结果:RAW264.7源性泡沫细胞经Pur处理后胆固醇摄取减少、胞内胆固醇外排增加并且自噬相关蛋白LC3II表达增加,P62表达减少,脂质吸收相关蛋白CD36表达减少,胆固醇外排相关蛋白ABCA1和LAL表达增加,其变化与自噬激动剂雷帕霉素处理后改变类似。Pur与自噬抑制剂3-甲基腺嘌呤共处理后,胆固醇摄取增加、胆固醇外排功能减弱,并且ABCA1、LAL和p-AMPK的蛋白水平减少,但CD36表达未有明显改变。结论:Pur可使得泡沫细胞LAL和ABCA1介导的胆固醇外排能力有所增强,其机制可能是通过AMPK通路增强自噬对胆固醇外排的调控;又可通过对CD36负向调节而使得泡沫细胞对脂质的摄取减少。     

苦参碱对人单核细胞内三磷酸腺苷结合盒转运体A1及胆固醇酯的影响

目的观察苦参碱对人单核细胞胆固醇和三磷酸腺苷结合盒转运体A1(ABCA1)表达的影响。方法用TBARS法检测细胞内MDA,油红O染色法检测泡沫细胞的形成,荧光分光光度法检测细胞内总胆固醇、游离胆固醇和胆固醇酯含量,RT-PCR和Western blot检测ABCA1mRNA与蛋白的表达。结果单核细胞经佛波脂(PMA)及ox-LDL共同孵育后,细胞内积聚的总胆固醇、游离胆固醇、胆固醇酯及脂质过氧化产物均明显增多(P<0.05),有大量泡沫细胞形成,而ABCA1蛋白、mRNA水平明显减少(P<0.05);苦参碱干预组显著缓解上述变化,细胞内胆固醇酯减少,ABCA1 mRNA、蛋白表达明显增加(P<0.05)。结论苦参碱可能通过增强ABCA1的表达和降低细胞内胆固醇聚积而发挥抗动脉粥样硬化的作用。     

温胆汤抑制氧化低密度脂蛋白诱导的巨噬细胞泡沫化

目的:从巨噬细胞泡沫化角度探讨温胆汤抗动脉粥样硬化的作用机制。方法:采用MTT法确定温胆汤的无毒性干预浓度;药物干预后,采用油红O染色法检测泡沫细胞的形成;采用酶法检测细胞内胆固醇水平及细胞胆固醇流出;采用Western blot检测巨噬细胞膜蛋白CD36、A类清道夫受体(SR-A)及ATP结合盒转运体A1(ABCA1)和B类I型清道夫受体(SR-BI)的蛋白表达水平。结果:温胆汤冻干粉溶液对氧化型低密度脂蛋白(oxLDL)刺激的THP-1细胞源性巨噬细胞的无毒性干预浓度为0～6 g/L,于是设置温胆汤干预浓度梯度为1.5、3和6 g/L进行后续实验。各浓度温胆汤干预可抑制ox-LDL诱导的泡沫细胞形成,半定量分析显示温胆汤浓度依赖性降低细胞内脂质沉积(P<0.05或P<0.01)。温胆汤浓度依赖性降低细胞内总胆固醇、胆固醇酯和胆固醇酯化率(P<0.05或P<0.01),促进细胞胆固醇流出(P<0.01),减少CD36表达(P<0.01)。6 g/L温胆汤显著减少SR-A的表达(P<0.05);3和6 g/L温胆汤显著促进ABCA1和SR-BI...     

甲基莲心碱对RAW264.7巨噬细胞泡沫化的影响

目的探讨甲基莲心碱(Nef)对巨噬泡沫细胞形成的作用及机制。方法采用体外培养小鼠巨噬细胞系RAW264.7细胞,ox-LDL诱导建立泡沫细胞模型,用Nef进行干预。油红O染色观察细胞内脂质堆积情况,酶比色法定量细胞内总胆固醇和胆固醇酯的变化,免疫荧光和流式细胞术分析CD36受体蛋白表达,RT-PCR检测CD36受体mRNA表达。结果与ox-LDL诱导组相比,Nef保护组巨噬细胞的油红O染色阳性细胞数和细胞内脂质含量均显著减少;同时CD36受体蛋白和mRNA表达明显降低。结论Nef可抑制ox—LDL诱导的RAW264.7巨噬细胞泡沫化,该作用可能与Nef下调巨噬细胞CD36受体表达,从而减弱巨噬细胞对ox—LDL的摄取有关。     

脂联素抑制甲基环糊精包被的胆固醇诱导的人血管平滑肌细胞泡沫化

目的:体外研究脂联素(APN)对甲基环糊精包被的胆固醇(Chol:MβCD)诱导的人血管平滑肌细胞(VSMCs)泡沫化过程的影响,并探讨其可能的机制。方法:体外培养人主动脉平滑肌细胞,将细胞分为5组,即对照组(正常培养VSMCs)、泡沫细胞组(VSMCs与100 mg/L Chol:MβCD共孵育24 h)及低、中、高剂量APN组(在泡沫细胞组基础上分别加入2.5、5和10 mg/L APN干预24 h)。油红O染色及脂质含量计算分析各组细胞泡沫化情况,酶荧光法检测胞内胆固醇含量及细胞胆固醇流出率。RT-qPCR和Western blot法分别检测各组细胞内沉默信息调节因子1(SIRT1)和过氧化物酶增殖物激活受体γ辅激活因子1α(PGC-1α)mRNA和蛋白的表达。结果:与对照组相比,泡沫细胞组胞质内可见大量红色脂滴聚集,胞核突出,胞内总胆固醇(TC)、游离胆固醇(FC)和胆固醇酯(CE)的含量及CE/TC值均显著升高(P0.05),细胞胆固醇流出率增加(P0.05),符合泡沫细胞改变;SIRT1和PGC-1α的mRNA和蛋白表达水平均显著降低(P0.05)。APN干预可呈浓度依赖性降低泡沫细胞内脂质沉积(P0.05),减少胞内TC和CE含量及CE/TC值(P0.05),促进细胞胆固醇流出(P0.05);SIRT1和PGC-1α的mRNA和蛋白表达水平随着APN浓度的升高而逐步升高(P0.05)。结论:APN在体外可呈浓度依赖性抑制Chol:MβCD诱导的VSMCs泡沫化,其机制可能与调控SIRT1/PGC-1α信号通路有关。     

血管紧张素-(1-7)通过AC-cAMP途径增加THP-1源性泡沫细胞ABCA1的表达并减少细胞胆固醇含量

目的: 构建THP-1源性泡沫细胞模型,探讨血管紧张素-(1-7) 和腺苷酸环化酶抑制剂MDL对THP-1源性泡沫细胞胆固醇逆转运相关蛋白——ATP结合盒转运子A1(ABCA1)表达和胆固醇含量的影响。方法: 将THP-1源性单核细胞诱导为巨噬细胞,加入氧化低密度脂蛋白(ox-LDL)建立泡沫细胞模型。实验分为4组:正常泡沫细胞组、MDL组、Ang-(1-7)组和MDL+Ang-(1-7)组。处理24 h后,分别通过ELISA法、荧光定量PCR法和Western blotting检测各组细胞cAMP表达水平、ABCA1 mRNA相对表达量和蛋白含量,闪烁计数法检测各组细胞胆固醇流出率,高效液相色谱法检测各组细胞胆固醇含量。结果: (1)Ang-(1-7)组cAMP表达水平、ABCA1 mRNA相对表达量和蛋白含量较泡沫细胞组有明显升高(P<0.05),胆固醇流出率较泡沫细胞组明显增高(P<0.05),胆固醇含量较泡沫细胞组显著降低(P<0.05)。(2)MDL组cAMP表达水平、ABCA1 mRNA相对表达量和蛋白含量较泡沫细胞组有明显降低(P<0.05),胆固醇流出率较泡沫细胞组明显降低(P<0.05),胆固醇含量较泡沫细胞组增高(P<0.05)。(3)MDL+Ang-(1-7)组数值介于Ang-(1-7)组与泡沫细胞组之间。结论: (1)Ang-(1-7)能促进泡沫细胞ABCA1的表达增加,促进胆固醇外流,减少泡沫细胞胆固醇含量,逆转泡沫细胞的形成。(2) MDL可以抑制腺苷酸环化酶的活性,减少cAMP的生成,从而部分拮抗Ang-(1-7)的上述作用,但不能完全阻断Ang-(1-7)的作用。     

冠心病患者来源HDL对小鼠腹腔巨噬细胞脂质沉积及凋亡的影响

目的:探讨冠心病(CAD)患者来源的高密度脂蛋白(HDL)对小鼠腹腔巨噬细胞脂质沉积及凋亡的影响。方法:分离提取健康人群、稳定型冠心病(SCAD)及急性心肌梗死(AMI)患者的HDL,并观察其对氧化型低密度脂蛋白(ox-LDL)诱导的泡沫细胞内脂质沉积及细胞凋亡的影响。采用油红O检测细胞内脂质沉积,用DCFH-DA检测活性氧簇(ROS)的水平,annexin V/PI检测巨噬细胞凋亡率,Western blot检测巨噬细胞ATP结合盒转运体(ABC)A1、ABCG1、Bcl-2和Bax的表达。结果:ox-LDL处理后巨噬细胞脂质沉积增加, ABCA1和ABCG1表达上调(P<0.05);与单纯ox-LDL处理组相比,联合健康人HDL(HDL_healthy)处理后巨噬细胞脂质沉积减少,ABCA1和ABCG1表达上调(P<0.05),而联合SCAD患者HDL(HDL_SCAD)或AMI患者HDL(HDL_AMI)处理后巨噬细胞脂质沉积进一步增加,ABCA1和ABCG1表达下调(P<0.05);与HDL_...     

下调牛磺酸调节基因1减轻氧化低密度脂蛋白诱导的血管内皮细胞氧化损伤

目的研究牛磺酸调节基因1(TUG1)对氧化低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮细胞(HUVECs)氧化损伤的影响。方法将HUVECs分为:对照组、ox-LDL组(含有100 g/mL的ox-LDL细胞培养液)、si-NC+干预组(转染siRNA阴性对照)和si-TUG1+干预组(转染TUG1 siRNA)。实时定量PCR检测HUVECs中TUG1表达;DCFH-DA法检测细胞活性氧(ROS)水平;WST法检测细胞超氧化物歧化酶(SOD)活性;TBA比色法检测培养液中丙二醛(MDA)含量;LD-P法检测培养液中乳酸脱氢酶(LDH)活性;硝酸还原酶法检测培养液中一氧化氮(NO)含量;流式细胞计量术检测细胞凋亡,Western blot检测细胞中c-caspase-3蛋白水平。结果 ox-LDL组HUVECs中TUG1表达水平较对照组升高(P0.05)。TUG1 siRNA转染可下调TUG1的表达(P0.05)。ox-LDL组HUVECs中的ROS水平升高(P0.05),SOD活性降低(P0.05),培养液中MDA含量升高(P0.05),LDH活性升高(P0.05),NO含量降低(P0.05),细胞凋亡率升高(P0.05),细胞中c-caspase-3蛋白水平升高(P0.05)。si-TUG1+干预组显著减轻ox-LDL组的上述变化(P0.05)。结论下调TUG1减轻ox-LDL诱导的HUVECs氧化损伤,减少HUVECs凋亡。     

可溶性Klotho蛋白抑制THP-1源性泡沫细胞形成

目的:探讨可溶性Klotho(KL)蛋白对THP-1源性泡沫细胞形成的影响。方法:THP-1单核细胞与160 nmol/L佛波酯孵育48 h后,诱导分化为THP-1源性巨噬细胞,给予THP-1源性巨噬细胞不同浓度(25、50、100和200μg/L)的可溶性KL蛋白预处理后,再由氧化型低密度脂蛋白(ox-LDL)诱导其转变为泡沫细胞。油红O染色观察细胞内脂滴形成情况,通过液体闪烁计数法测定THP-1源性泡沫细胞内胆固醇流出情况,酶荧光法检测细胞内游离胆固醇及胆固醇酯的含量,并分别用Western blot法及RT-qPCR法检测酰基辅酶A:胆固醇酰基转移酶1(ACAT1)和ATP结合盒转运体A1(ABCA1)的蛋白及mRNA表达。结果:可溶性KL蛋白能增加THP-1源性巨噬细胞内胆固醇流出,抑制THP-1源性泡沫细胞形成,且可溶性KL蛋白呈一定浓度依赖性地抑制ox-LDL诱导的泡沫细胞形成过程中ACAT1表达的上调和ABCA1表达的下调(P<0.05)。结论:可溶性KL蛋白能够抑制THP-1源性泡沫细胞形成,其机制可能与靶向调控细胞内ACAT1和ABCA1的表达有关。     

Immunoregulatory function of mesenchymal stem cells

Mesenchymal stem cells (MSC) are a rare subset of stem cells residing in the bone marrow where they closely interact with hematopoietic stem cells and support their growth and differentiation. MSC can differentiate into multiple mesenchymal and non-mesenchymal lineages, providing a promising tool for tissue repair. In addition, MSC suppress many T cell, B cell and NK cell functions and may affect also dendritic cell activities. Due to their limited immunogenicity, MSC are poorly recognized by HLA-incompatible hosts. Based on these unique properties, MSC are currently under investigation for their possible use to treat immuno-mediated diseases. However, both their condition of immunoprivilege and their immunosuppressive function have recently been challenged when analyzed under particular experimental conditions. Thus, it is likely that MSC effects on the immune system may be deeply influenced not only by cell-to-cell interactions, but also by environmental factors shaping their phenotype and functions.     

Influences of the pia mater on the precursors of nerve cells

Summary In the developing cerebellum of 8-day old rats surgical lesions were made. During regeneration of the cerebellum the pia mater was found to penetrate inside the neural tissue. Partially differentiated Purkinje cells and granule cells, that were in close contact with the pial cells, were found atrophied. When the profilerative cells of external granular layer came into contact with the pial cells, they were reduced to a primitive type of epitheloid cells. In this instance epithelio-mesenchymal interaction was found deleterious to the precursors of neurons. However, when the epithelioid cells were freed from the contact with the pial cells by intervening basement membrane, they differentiated into ependymal cells. Such ependymal cells gave rise to small as well as large new ventriculer structures, and structures resembling chorioid plexus.This research was supported by NIH Research Grant NS08817-03. Acknowledgements are due to Sheila Anderson for histology and to Donna Whitehurst for photographic work.     

干细胞若干定义的相关探讨

背景：近10年来干细胞研究所取得的巨大进展,不仅影响和促进了生物学及其相关基础科学,而且还在医学、药物开发、农业等许多领域得到广泛的应用,目前已成为研究的热点问题。 目的：在干细胞的定义上还存在一些需要探讨的问题,明确定义将有利于干细胞研究的快速发展。 方法：回顾干细胞的发展和概念提出,特别是通过中英文权威定义的比较,提出作者的看法。 结果与结论：干细胞的定义上还存在一些问题值得探讨,特别是一些中英文表述不同影响了理解。例如,“多能干细胞”对应译成两个单词：Pluripotent Stem Cell和Multipotent Stem Cell,然而Pluripotent和Multipotent两个单词的英文意义不同。为了学术交流和沟通,作者提出将Pluripotent Stem Cell称为“万能干细胞”,而保留Multipotent Stem Cell为“多能干细胞”的定义。以上结论供广大同仁探讨,以利于抛砖引玉。中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程全文链接：     

Genetic aspects of the immunomodulationg action of interferon

Laboratory of Antiviral Immunity, N. F. Gamaleya Institute of Epidemiology and Microbiology, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR V. D. Solov'ev.) Translated from Byulleten' Éksperimentl'noioi Biologii i Meditsiny, Vol. 105, No. 4, pp. 459–461, April, 1988.     

Identification of a CD8α Dendritic Cell Subpopulation in Rat Spleen and Evaluation of Its OX-62 Expression

Rat spleen DC and bone marrow-derived DC were isolated and characterized by morphology and flow cytometry. We found a CD8α⁺ DC subpopulation representing 19–48% (27.4 ± 12.0) of total spleen DC. The OX-62 expression on total spleen DC was 41–59% (51.8 ± 7.5). Myeloid bone marrow-derived DC were negative for CD8α and OX-62. We demonstrated the coexpression of CD8α and OX-62 molecules, at least in a portion CD8α⁺ spleen DC. Both CD8α⁺ and CD8α⁻ spleen DC subpopulations separated by MACS were able to induce an in vivo primary immune response to OVA. The immune response induced by the CD8α⁻ DC subpopulation was higher (P < 0.05). We identified a CD8α⁺ DC subpopulation in rat spleen less effective in inducing an immune response than CD8α⁻ DC. Moreover, our results suggest the presence of DC subpopulations with different lineages in DC preparations based on OX-62 expression.     

Accessory phenotype and function of macrophages induced by cyclic adenosine monophosphate

Mature macrophages (Mph) differentiated in culture from normal human peripheral blood monocytes (Mo) exhibit low activity as accessory cells (antigen-presenting cells) in T lymphocyte stimulation. A test system was established based on mitogenicity to quantitate the accessory activity of Mph-derived cells and to follow its changes for several days. The system used accessory cells treated with the oxidative mitogen, sodium periodate. The cells were subsequently co-cultured with pooled human lymphocytes from a cryopreserved stock. DNA synthesis in these cells was used as an indicator of accessory activity. Mph could be converted within 5-6 days into highly active accessory cells if a continuous stimulus of exogenously added dibutyryl cyclic AMP (db-cAMP) was provided. Mph treated by db-cAMP retained a high degree of HLA-DR expression but typical Mph markers such as non-specific esterase, phagocytosis, and expression of Fc-receptors were down-regulated. Acid phosphatase and myeloperoxidase underwent only slight changes, while the monocyte marker 5'-nucleotidase remained undetectable. Morphologically, the cells rounded up and developed veils and dendritiform elongations. In contrast to dendritic cells, Mph-derived accessory cells retained the CD14 antigen characteristic of monocytes and Mph. It is concluded that Mph are able to respond to exogenous stimuli and to convert into a highly active accessory cell. This contrasts to the well-known state of the 'activated Mph' with respect to markers and function. Both states appear to be antagonistically controlled by intracellular second messengers, as the accessory cell phenotype is positively correlated with intracellular cyclic AMP increase, whereas Mph activation correlates with cyclic GMP increase.     

Human Osteogenic Sarcoma: Fine Structure of the Osteoblastic Type

The fine structure of representative regions of 13 osteoblastic osteogenic sarcomas was studied. These regions contained four morphologically distinguishable subtypes of osteoblastlike cells. In addition, fibroblastlike and chondroblastlike cells were present, along with multinucleated giant cells, leukocytes, macrophagelike cells, and small populations of histogenetically unclassifiable (but probably neoplastic) cells.

The morphologic evidence was compatible with the view that the variations in appearance among the subgroups of osteobl astlike cells reflected differences in maturation and differentiation of these cells. In at least one subgroup, the morphologic findings suggested that the ceils were capable of manufacturing a secretory product. The multinucleated giant cells occurring in genuine tumor areas appeared to be closely related to neoplastic osteoblasts.

The presence of chondroblastlike cells in the tissues illustrates that cells with a diverging differentiation can occur in an osteoblast-dominated cell population. This agrees with the view that the neoplastic cells originate from a mesenchymal stem cell with potential for multifaceted differentiation.     

Trypanosoma cruzi calreticulin: In vitro modulation of key immunogenic markers of both canine tumors and relevant immune competent cells

Recombinant calreticulin from Trypanosoma cruzi (rTcCalr), the parasite responsible for Chagas’ disease, binds to Canine Transmissible Venereal Tumor (CTVT) cells from primary cultures and to a canine mammary carcinoma cell line. A Complement-binding assay indicated that interaction of the first component C1q with these tumor cells operated independently of the rTcCalr-presence. This apparent independence could be explained by the important structural similarities that exist among rTcCarl, endogenous normal canine and/or mutated calreticulins present in several types of cancer. In phagocytosis assays, tumor cells treated with rTcCalr were readily engulfed by macrophages and, co-cultured with DCs, accelerated their maturation. In addition, DCs maturation, induced by tumor cells co-cultured with rTcCalr, activated T cells more efficiently than DCs, treated or not with LPS. In an apparent paradox, a decrease in MHC Class I expression was observed when these tumor cells were co-cultivated with rTcCalr. This decrease may be related to a down regulation signaling promoting the rescue of MHC I. Possibly, these in vitro assays may be valid correlates of in vivo sceneries. Based on these results, we propose that rTcCalr improves in vitro the immunogenicity of two widely different tumor cell lines, thus suggesting that the interesting properties of rTcCalr to boost immune responses warrant future studies.     

雪峰虫草对DC-CIK增殖及HepG-2细胞杀伤作用的实验研究

目的:研究雪峰虫草对DC-CIK细胞增殖及肝癌Hep G-2细胞杀伤作用。方法:常规分离健康人外周血单个核细胞并诱导生成DC细胞和CIK细胞,将DC细胞与CIK细胞按1∶5共培养7 d后给药组加入不同浓度的雪峰虫草水提取物,第10天观察形态并计数各组DC-CIK细胞;收集培养第10天的DC-CIK细胞作为效应细胞,对数生长期Hep G-2肝癌细胞作为靶细胞,使Hep G-2∶DC-CIK靶效比为1∶5,cck-8法检测DC-CIK对Hep G-2的杀伤率。结果:雪峰虫草水提物组对DC-CIK有显著的促增长作用,其作用的最佳浓度为0.1 mg/ml。雪峰虫草诱导的DC-CIK细胞对肝癌Hep G-2细胞的杀伤作用优于常规方法培养的DC-CIK细胞;常规方法培养的DC-CIK细胞加雪峰虫草与常规方法培养的DC-CIK细胞对肝癌Hep G-2细胞的杀伤作用无显著性差异,雪峰虫草体外直接杀伤肝癌Hep G-2细胞的作用不明显。结论:雪峰虫草通过促进DC-CIK细胞增殖而增强其杀伤肝癌Hep G-2细胞的作用。