脾酪氨酸激酶蛋白在食管鳞癌组织中的表达及临床意义

目的观察脾酪氨酸激酶（Syk）蛋白在食管鳞癌组织中的表达,探讨其与食管鳞癌发生、发展的关系。方法用免疫组化法检测48例食管鳞癌患者的癌组织及其癌旁组织中的Syk蛋白表达;分析不同TNM分期及淋巴结转移情况下食管鳞癌组织中的Syk蛋白表达。结果食管鳞癌组织Syk蛋白表达阳性率显著低于癌旁组织（χ^2=51.24,P〈0.05）;Syk蛋白表达与肿瘤TNM分期及淋巴结转移相关（P〈0.05）。结论食管鳞癌组织中Syk蛋白表达缺失与其发生及淋巴结转移倾向有关。     

Neuropilin-1在食管鳞癌中的表达及其意义

目的探讨Neuropilin-1(NRP-1)在食管鳞癌中的表达及其生物学和临床意义.方法取42例新鲜原发食管鳞癌和邻近正常食管粘膜标本,所有的标本均经病理诊断确诊.用RT-PCR方法检测每例患者癌组织和相应癌旁正常组织中NRP-1mRNA的表达.癌与癌旁正常组织表达值之间的比较采用配对t检验,癌组织的表达值与临床病理因素之间的关系采用成组t检验,检验结果以P＜0.05为有意义.结果在42例患者中,37例NRP-1mRNA表达强于相应癌旁正常组织,占89.00%,肿瘤直径≥3 cm组、外膜浸润组和淋巴结转移组的NRP-1mRNA表达明显增高.癌组织中NRP-1mRNA的表达与肿瘤分化程度和性别无关.结论在食管鳞癌中NRP-1mRNA的表达明显高于癌旁正常组织,并且与食管鳞癌的发展、浸润和转移有关.     

Cyclin B1、CDK1在食管鳞癌组织中的表达及临床意义

目的: 探讨食管鳞癌组织中细胞周期蛋白Cyclin B1和细胞周期蛋白依赖性激酶CDK1表达及其临床病理学意义.方法: 应用免疫组织化学SP法对52例食管鳞癌(组织学Ⅰ级8例, Ⅱ级20例, Ⅲ级24例;有淋巴结转移20例, 无淋巴结转移32例;原位癌16例, 侵袭性癌36例包括浸润至黏膜下层、肌层、全层)及其配对的癌旁正常组织进行Cyclin B1、CDK1的检测, 分析其阳性表达与食管鳞癌患者临床病理因素的关系.结果: 食管鳞癌组织中Cyclin B1、CDK1的阳性表达高于癌旁正常食管黏膜组织, 2组差异都有统计学意义(71.2% vs 2.0%, 65.4%vs 3.9%, 均P <0.05). 食管鳞癌组织中CyclinB1、CDK1的表达都与性别、年龄无关;与组织学分级、浸润深度及淋巴结转移有关(均P <0.05). Cyclin B1阳性表达强度与CDK1的阳性表达强度之间呈正相关(r = 0.697, P <0.05) .结论: Cyclin B1、CDK1的高表达会促进食管鳞癌的发生与发展. 而且食管鳞癌中CyclinB1与CDK1的表达密切相关, 可作为食管鳞癌生物学行为预测的参考指标.     

脾酪氨酸激酶蛋白在胃癌组织中的表达及意义

目的观察脾酪氨酸激酶（Syk）蛋白在胃癌组织中的表达,探讨其与胃癌发生、发展的关系。方法采用免疫组化法检测38例胃癌患者的癌组织及16例癌旁组织中Syk蛋白表达,并分析不同组织学类型、TNM分期、淋巴结转移、浆膜浸润、远处转移等胃癌组织中Syk蛋白的表达情况。结果胃癌组织中Syk蛋白表达阳性率显著低于癌旁组织（P〈0.01）;Syk蛋白表达与肿瘤TNM分期、淋巴结转移、浆膜浸润及远处转移相关（P均〈0.05）。结论胃癌组织中Syk蛋白表达缺失与其发生恶性生物学倾向有关。     

Ang-2和Tie-2在食管鳞癌患者肿瘤组织中的表达及意义

食管鳞癌(ESCC)是常见的消化系统恶性肿瘤,死亡率较高,严重威胁人民健康与生命.食管癌浸润、转移是引起死亡的主要原因,尤其血管生成是食管癌发生侵袭和转移的关键点.血管生成素/内皮细胞酪氨酸激酶受体系统(Angiopoietins/Tie-2)是近年来发现的在血管新生过程中调节血管生成、重塑并影响其成熟和稳定性的一类重要配体/受体系统,其生物学行为和作用机制尚在探索之中[1].     

SOX2在食管鳞癌组织中的表达及意义

目的:探讨食管鳞癌组织中SOX2 mRNA和蛋白的表达及意义.方法:应用原位杂交和免疫组织化学法分别检测35例正常食管黏膜组织及84例食管鳞癌组织中SOX2 mRNA和蛋白的表达.结果:SOX2在正常黏膜组织中mRNA的表达率为8.6％(3/35),显著低于其在癌组织中的表达率46.4％(39/84),组间比较差异具有...     

抑癌基因P16在肺鳞癌和肺腺癌中的表达及临床意义

目的 探讨p16基因产物在肺鳞癌和肺腺癌中的表达及其意义。方法 本组56例原发性非小细胞肺癌，其中鳞癌37例，腺癌19例。用免疫组织化学PCR法检测患者肺癌新鲜标本p16蛋白表达水平。结果 56例肺癌标本中p16蛋白阳性表达率为58.9%（33／56），伴有淋巴结转移者其阳性表达率41.4%（12／29）显著低于无淋巴结转移者（P16阳性表达率为77.8%（21／27），P＜0.01）。P16蛋白阴性表达者的1年、3年生存率分别为59.7%、44.1%，显著低于p16蛋白阳性表达者85.2%、71.8%。结论 p16蛋白表达与肺鳞癌和肺腺癌的组织类型，淋巴结转移及预后有关。p16蛋白状态可作为判断肺癌预后的指标之一。     

食管鳞癌组织中抑癌基因SEMA3B的表达及意义

目的 探讨抑癌基因信号素3B(SEMA3B)在食管鳞癌(ESCC)发生、发展中的作用.方法 采用RT-PCR法检测51例食管鳞癌组织(观察组)及相应正常食管组织(对照组)中SEMA3B基因的表达,并分析其与食管鳞癌临床病理参数的关系.结果 对照组SEMA3B基因均表达,观察组SEMA3B基因表达缺失率为58.8%(30/51,P＜0.05);SEMA3B基因表达与食管鳞癌组织发生部位、淋巴结转移和临床病理分期有关,而与年龄、性别、肿瘤细胞分化程度和大体分型无关.结论 SEMA3B基因的表达异常可能在食管鳞癌的发生、发展及预后中起重要作用;SEMA3B可作为反映食管鳞癌生物学行为的指标之一.     

Clinical significance of matrix metalloproteinase-9 expression in esophageal squamous cell carcinoma

AIM: To evaluate the expression of matrix metalloproteinase-9 (MMP-9) and its clinical significance in esophageal squamous cell carcinoma (ESCC). METHODS: The expression of MMP-9 in 208 cases of ESCC was detected by immunohistochemistry (IHC) and its clinical significance in ESCC especially the relationship with the clinicopathological parameters was analyzed. RESULTS: The percentage of positive cases for MMP-9 detected by IHC was 49.0%. MMP-9 was mainly expressed in the cytoplasm of cancer cells especially in the invasive front. Only weak expression was detected in the stromal cells and no expression in non-cancerous mucosa. The expression of MMP-9 was positively correlated with poorer differentiation (P= 0.001<0.01), existence of vessel permeation (P= 0.027<0.05) and lymph node metastasis (P=0.027<0.05). CONCLUSION: The expression of MMP-9 correlates with the cancer cell differentiation, vessel permeation and lymph node metastasis. It may be a novel biomarker for the diagnosis and treatment of ESCC.     

Expression of OCT4 in human esophageal squamous cell carcinoma is significantly associated with poorer prognosis

AIM: To explore the expression pattern of OCT4 in human esophageal squamous cell carcinoma and its significance in diagnosis and prognosis.METHODS: Using real-time polymerase chain reaction (PCR), Western blotting, immunocytochemistry and immunohistochemistry, the expression of OCT4 in three esophageal squamous cancer cell lines, KYSE70, KYSE140 and KYSE450, was characterized. OCT4 expression was investigated in a series of 153 esophageal squamous cell carcinoma samples using immunohistochemistry and explored its association with clinicopathological features.RESULTS: Immunohistochemically, OCT4 positive immunostaining was observed in cancer cell nuclei. OCT4 was variably expressed in three esophageal squamous cancer cell lines. Among 153 specimens, 105 (68.7%) were negative or weakly positive for OCT4 staining; 21 (13.7%) were moderately positive and 27 (17.6%) were strongly positive. Higher expression level of OCT4 was significantly associated with higher histological grade (P < 0.001) and poor clinic outcome (P < 0.001).CONCLUSION: The expression of OCT4 enables the tumor to have a higher degree of stemness, which in turn results in a poorer clinical outcome for patients with esophageal squamous cell carcinoma.     

TWEAK及其受体Fn14蛋白在食管鳞癌中的表达及临床病理意义

目的:探讨肿瘤坏死因子样凋亡微弱诱导剂(TNF-like weak inducer of apoptosis,TWEAK)及其受体早期反应蛋白14(fibroblast growth factor-inducible 14,Fn14)表达与食管鳞状细胞癌发生、发展及浸润、转移的关系.方法:应用免疫组化法分别检测手术切除的45例食管鳞癌组织、22例癌旁不典型增生组织及22例正常食管黏膜组织中TWEAK及Fn14蛋白表达,并且对二者在食管鳞状细胞癌组织中的表达情况进行相关性分析.结果:食管鳞癌组织中TWEAK蛋白表达与癌的浸润深度、淋巴结转移及TNM分期均密切相关(χ2分别为6.455,11.645及4.185,P均<0.05);在食管鳞癌癌变过程中TWEAK蛋白表达在正常黏膜组织、癌旁不典型增生组织及癌组织中的表达率依次增高,分别为27.3%(6/22)、40.9%(9/22)、64.4%(29/45),组间比较差异具有统计学意义(χ2=9.018,P<0.05);Fn14蛋白表达也与癌的组织学分级、浸润深度、淋巴结转移及TNM分期均密切相关(χ2分别为10.873、12.513、9.244及13.137;均P...     

Smo和Gli1蛋白在食管鳞状细胞癌组织中的表达及临床意义

目的:探讨Sonic hedgehogq信号通路中Smo和Glil蛋白在食管鳞状细胞癌(ESCC)组织中的表达及临床意义.方法:采用免疫组织化学二步法分别检测60例ESCC、45例癌旁异型增生及60例食管正常黏膜组织中Smo和Glil蛋白的表达,并分析两者的表达水平与临床病理因素的关系.结果:Smo和Glil蛋白在ES...     

mTOR和p-p70S6K在食管鳞癌组织中蛋白表达的相关性及其临床意义

目的:探讨哺乳动物雷帕霉素靶蛋白(mTOR)及其下游关键因子p-p70S6K的表达与食管鳞癌发生、发展及浸润、转移的关系,方法:35例食管癌手术切除标本取自河南省安阳市肿瘤医院,应用免疫组织化学SP法检测35例食管鳞癌组织,15例癌旁不典型增生组织及15例正常食管黏膜组织中mTOR及p-p70S6K蛋白的表达.结果:食...     

Expression of thymidylate synthase and glutathione-s- transferase π in patients with esophageal squamous cell carcinoma

AIM： To investigate the expression of thymidylate synthase （TS） and glutathione-s-transferase π （GST-π） in esophageal squamous cell carcinoma and their association with the clinicopathologic characteristics. METHODS： Immunohistochemical methods were used to detect the expression of TS and GST-π in surgically resected formalin-fixed, paraffin-embedded esophageal squamous cell carcinoma （ESCC） tissue sections from 102 patients （median age, 58 years） and in 28 normal esophageal mucosa （NEM） samples. The relationship between TS and GST-π expression and clinicopathologic factors was examined. RESULTS： The expression of TS and GST-π was not statistically significantly associated with age of the patients, tumor size, lymph node metastasis, depth of invasion or tumor stage. TS staining was positive in 17.86% of normal esophageal mucosa and in 42.16% of ESCC samples （P 〈 0.05）. The expression level of TS was not only significantly lower in well-differentiated （21.88%） than in poorly-differentiated carcinomas （51.43%, P 〈 0.05）, but was also significantly higher in samples from male patients （46.51%） than from female patients （18.75%, P 〈 0.05）. GST-π was positively stained in 78.57% of normal esophageal mucosa and in 53.92% of ESCC samples （P 〈 0.05）. The expression level of GST-π was also significantly higher in welldifferentiated carcinomas （65.63%） than in poorly- differentiated carcinomas （35.00%, P 〈 0.05）. CONCLUSION： The expression of TS and of GST-π may be used as molecular markers for the characterization of ESCC. Poorly-differentiated cells showed increased expression of T5 and reduced expression of GST-π.