HPLC测定复方卵磷脂软胶囊中B族维生素的含量

目的 :建立反相高效液相色谱法测定复方卵磷脂软胶囊中 B族维生素含量的方法。方法 :采用 Agilent C1 8色谱柱(2 5 0 mm× 4 .6 m m ) ,以乙腈 -甲醇 -水 (其中含 2 .5 m mol/ L庚烷磺酸钠、0 .12 %三乙胺 ,磷酸调节至 p H 2 .5 ) (12∶ 6∶ 82 )为流动相 ;流量 1.5 ml/ min,检测波长为 2 73nm。结果 :B族维生素含量测定方法的线性良好 ,烟酰胺、维生素 B6 和维生素 B1 、维生素 B2 的平均回收率分别为 10 0 .0 % ,10 0 .1% ,99.7% ,99.9% ,符合分析要求。结论 :为复方卵磷脂软胶囊中 B族维生素成分的含量测定提供了一个灵敏、准确和快速的测定方法     

HPLC测定复方卵磷脂软胶囊中B族维生素的含量

目的:建立反相高效液相色谱法测定复方卵磷脂软胶囊中B族维生素含量的方法。方法:采用Agilent C₁₈色谱柱(250mm×4.6mm),以乙腈-甲醇-水(其中含2.5mmol/L庚烷磺酸钠、0.12%三乙胺,磷酸调节至pH2.5)(12∶6∶82)为流动相;流量1.5ml/min,检测波长为273nm。结果:B族维生素含量测定方法的线性良好,烟酰胺、维生素B₆和维生素B₁、维生素B₂的平均回收率分别为100.0%,100.1%,99.7%,99.9%,符合分析要求。结论:为复方卵磷脂软胶囊中B族维生素成分的含量测定提供了一个灵敏、准确和快速的测定方法     

复方维生素E霜中熊果苷和维生素E的含量测定

目的建立复方维生素E霜中熊果苷和维生素E的含量测定方法。方法熊果苷的含量测定采用正相高效液相色谱法,色谱柱为Machedey-NagelSi柱（250mm×4.6mm,5μm）,流动相为正己烷-醋酸乙酯-甲醇（30∶10∶9）,检测波长285nm,流速1.2mL/min,柱温35℃,进样量10μL。维生素E的含量测定采用反相高效液相色谱法,色谱柱为C18柱（150mm×4.6mm,5μm）,流动相为甲醇,检测波长285nm,流速1.5mL/min,柱温35℃,进样量10μL。结果熊果苷质量浓度在14.78～25.48μg/mL范围内与峰面积线性关系良好（r=0.9998）,平均加样回收率为100.50%,RSD=2.06%（n=9）;维生素E质量浓度在14.42～26.78μg/mL范围内与峰面积线性关系良好（r=0.9997）,平均加样回收率为97.21%,RSD=2.64%（n=9）。结论所用含量测定方法操作简便快速,结果准确可靠,可用于复方维生素E霜的质量控制。     

HPLC法测定穿心莲片中穿心莲内酯的含量

目的　对穿心莲片中穿心莲内酯的含量测定方法进行研究。方法　反相高效液相色谱法,YWG -C18色谱柱(2 5 0mm× 4 6mm,10 μm),流动相为甲醇 -水(5 0∶5 0 ),流速 1 2ml·min-1,检测波长 2 2 5nm。结果　平均回收率 99 4 %(n =5 )。结论　建立了一种操作简便、重现性良好的高效液相色谱法测定穿心莲片中穿心莲内酯的含量。     

反相高效液相色谱法测定复方呋喃西林滴鼻剂的含量

目的 :建立测定复方呋喃西林滴鼻剂中呋喃西林和盐酸麻黄素含量的反相高效液相色谱法。方法 :色谱柱 :KromasilC18(4 6mm×250mm ,5μm) ;流动相 :甲醇 -水 -三乙胺 -冰醋酸 (12∶88∶0 2∶1) ;内标 :氯霉素 ;检测波长 :254nm。结果 :复方呋喃西林滴鼻剂各组分及内标在18min内获得满意的分离 ,呋喃西林和盐酸麻黄素在定量范围内具有良好的线性关系和回收率。结论 :反相高效液相色谱法应用于复方呋喃西林滴鼻剂的含量测定 ,操作简便易行 ,结果准确可靠     

注射用复合维生素冻干粉针中10种维生素的含量测定

目的研究注射用复合维生素冻干粉针中水溶性维生素的提取方法,分别建立该制剂中6种水溶性维生素和4种脂溶性维生素含量测定的反相高效液相色谱法。方法水溶性维生素含量测定采用DiamonsilTM C18色谱柱(4.6 mm×20 mm,5μm);流动相A为甲醇,流动相B为20 mmol·L-1磷酸二氢钾缓冲盐(磷酸调pH值4.0),梯度洗脱;流速1.0 mL·min-1;以214 nm和254 nm为检测波长,双波长检测。脂溶性维生素含量测定采用Tigerkin C18色谱柱(4.6 mm×20 mm,5μm);流动相为甲醇-异丙醇(体积比95∶5);检测波长235 nm;流速1.0 mL·min-1。结果各种维生素含量测定方法的线性关系良好,相关系数为0.999 6～1.000,回收率98.1%～101.9%,RSD为0.5%～1.9%。结论实现了对亚微乳剂中水溶性维生素的提取分离,含量测定方法简单快速,精密度好,结果准确可靠。     

甘肃道地药材丹参提取工艺研究

目的 :建立甘肃道地药材丹参的最佳提取工艺及含量测定方法。方法 :采用不同的提取方法及反相高效液相色谱法对丹参进行分离分析 ,以ODS为色谱柱 ,测定脂溶性成分的流动相为甲醇 -水 (70∶30) ,检测波长为254nm ;测定水溶性成分的流动相为甲醇 -水 (8∶92) ,检测波长为282nm。结果 :在选定的色谱条件下 ,脂溶性和水溶性成分分离较好 ,并可进行含量测定。结论 :所建立的丹参中脂溶性和水溶性成分的提取方法 ,可用于甘肃不同地区产丹参的质量控制。     

高效液相色谱法测定复方亚油酸乙酯胶丸中橙皮苷的含量

目的建立复方亚油酸乙酯胶丸中橙皮苷的含量测定方法。方法液相色谱法。色谱柱:Kromasil C18柱(4.6mm×250mm,5μm);流动相:甲醇-0.5%冰醋酸溶液(40∶60);流速1.0mL.min-1;柱温:40℃;检测波长:283nm。结果橙皮苷在0.07505～0.3752μg范围内呈线性关系(r=0.9997,n=5),平均加样回收率为99.16%,RSD为1.7%(n=6)。结论该方法操作简便,结果可靠,可用于复方亚油酸乙酯胶丸中橙皮苷的含量测定。     

复方玉驹胶囊的质量标准研究

目的 :建立复方玉驹胶囊的鉴别与含量测定方法。方法 :分别以白蚁巢标准药材和乌药内酯为对照 ,采用薄层色谱法进行鉴别 ;反相高效液相色谱法测定含量 ,色谱柱为Shim packCLC ODS柱 ,检测波长 2 0 8nm ,流动相为乙腈 含0 1%磷酸的水 (5 0∶5 0 ) ,柱温为室温 ,流速 0 5ml·min 1。结果 :薄层色谱鉴别法专属性强 ,HPLC法测定含量精密度高 ,重复性好 (RSD <1 5 % ) ,平均回收率为 10 0 8% ,标准曲线回归方程为Y =- 40 19 3 +5 978C ,r =0 9999。结论 :鉴别与含量测定方法简便、准确 ,可用于复方玉驹胶囊的质量控制     

反相高效液相色谱法同时测定复方环丙沙星注射液中两组分的含量

目的 :测定复方环丙沙星注射液中环丙沙星和替硝唑的含量。方法 :采用反相 高效液相色谱法 ,色谱柱C18柱 ,流动相为 0 .4mol·L-1柠檬酸 甲醇 乙腈 (2∶1∶0 .8) ,流速为 0 .8ml·min-1,检测波长为 2 77nm。结果 :环丙沙星和替硝唑的回收率分别为 99.7%和 99.6 % ,RSD分别为 0 .9%和 0 .7%。结论 :方法快速、准确 ,可作为该复方制剂的质量控制标准     

Final report on the safety assessment of Lecithin and Hydrogenated Lecithin

Lecithin is a naturally occurring mixture of the diglycerides of stearic, palmitic, and oleic acids, linked to the choline ester of phosphoric acid, commonly called phosphatidylcholine. Hydrogenated Lecithin is the product of controlled hydrogenation of Lecithin. Bilayers of these phospholipids in water may form liposomes, a spherical structure in which the acyl chains are inside and not exposed to the aqueous phase. Lecithin and Hydrogenated Lecithin are used in a large number of cosmetic formulations as skin conditioning agents-miscellaneous and as surfactant-emulsifying agents. Hydrogenated Lecithin is also used as a suspending agent-nonsurfactant. Historical data on concentration of use of Lecithin reveals that 0.1% to 1.0% is the concentration range most frequently seen, with concentrations up to 50% reported for two moisturizing products. A solution of 65% Lecithin is currently reported to be used at concentrations up to 3% in cosmetics. Nonocclusive application of Lecithin-containing liposomes to murine skin resulted in 30% penetration to the subdermis. In piglet skin, the same application resulted in 99% accumulating in the stratum corneum. In general, liposomes are considered effective in capturing other compounds inside their spherical structure and delivering any such captured compound through the skin barrier. As a result, caution should be exhibited in formulating cosmetic products that contain these ingredients in combination with other ingredients whose safety is based on their lack of absorption or where dermal absorption is a concern. Lecithin is virtually nontoxic in acute oral studies, short-term oral studies, and subchronic dermal studies in animals. Lecithin is not a reproductive toxicant, nor is it mutagenic in several assays. In an oral carcinogenicity study, brain neoplasms were found in mice exposed to Lecithin. In a subcutaneous carcinogenicity study, no neoplasms were found in mice and rats exposed to Lecithin. Adverse reactions to Lecithin in a metered-dose inhaler have been reported. Lecithin and Hydrogenated Lecithin were generally nonirritating and nonsensitizing in animal and human skin. Based on the available data, Lecithin and Hydrogenated Lecithin are safe as used in rinse-off cosmetic products; they may be safely used in leave-on products at concentrations up to 15%, the highest concentration tested in clinical irritation and sensitization studies; but the safety of use could not be substantiated in cosmetic products likely to be inhaled. Because of the possibility of formation of nitrosamines, these ingredients should not be used in cosmetic products in which N-nitroso compounds may be formed.     

Bindung ringsubstituierter Benzylamine an Lecithin

Interaction of Ring Substituted Benzylamines with Lecithin The interaction of phospholipids with catamphiphilic drugs, molecular basis of undesired side effects, was investigated by NMR binding studies with 18 ring substituted benzylamines and lecithin. In order to determine this interaction quantitatively, the NMR line broadening of aromatic and methylene protons of 2 – 19 in solution was measured in the presence of lecithin. The excellent correlation between binding values and the substituent constants π of the model compounds demonstrates a linear increase of lecithin complexation with the lipophilic properties of the ring substituents.     

荧光光度法测定血清中卵磷脂的含量

目的:建立测定卵磷脂(PC)含量的荧光光度法。方法:以多西环素(DC)-铕(Eu3+)为荧光探针,在pH7.1条件下,扫描不同浓度的PC与DC-Eu3+的荧光光谱,优化测定PC含量的最佳试验条件。结果:主要试验条件最佳值为DC浓度3.0×10-6mol.L-1,Eu3+浓度1.0×10-5mol.L-1,PC检测浓度线性范围为4.0×10-7～2.4×10-5mol.L-1,检测限为3.1×10-7mol.L-1。结论:该方法能成功地应用于实际样品的含量测定。     

Topical Delivery of Flurbiprofen from Pluronic Lecithin Organogel

The purpose of this research is to formulate and evaluate the suitability of pluronic lecithin organogels containing flurbiprofen for topical application. Four formulations were developed using flurbiprofen, lecithin, Pluronic F127, isopropyl palmitate, water, sorbic acid and potassium sorbate were coded as FL1, FL2, FL3 and FL4. All the formulations carried 30% w/w of lecithin phase and 70% w/w of Pluronic phase. The formulated organogels were evaluated for appearance and feel psychorheologically, in vitro diffusion study, drug content, viscosity and pH. Release of flurbiprofen from all formulations was monitored via dialysis membrane-70 and Wistar rat skin as a semipermeable membrane into phosphate buffer saline (0.2 M, pH 7.4) using Keshary-Chien diffusion cell. The viscosities of different formulations were determined by using Brookfield Viscometer at 25°. An attempt has been made to explore the potential of pluronic lecithin organogels for topical delivery of flurbiprofen.     

Lecithin vesicles for topical delivery of diclofenac.

Skin penetration of topically applied diclofenac is important for the treatment of rheumatic diseases and actinic keratoses. We have studied the permeation of diclofenac across human cadaver epidermis in-vitro from four lecithin vesicle formulations and a few marketed semi-solid preparations. The lecithin vesicle formulations were prepared by dissolving the lipid contents (lecithin and sodium cholate) in a 1:1 mixture of methanol-chloroform, evaporating the solvents under vacuum, and hydrating the lipid layer with the drug solution in water or 10% ethanol. The vesicles were sonicated for 5 min to reduce the vesicle size and their size and Zeta potential were characterized. The cumulative amount and maximum flux of diclofenac was 69.7+/-40.3 micrograms and 4.77+/-3.16 micrograms/hcm(2) from lecithin vesicles containing sodium cholate and 10% ethanol, and is the highest of all formulations studied. The cumulative amount and mean maximum flux obtained from other formulations were in the range of 2.46+/-1.98-29.9+/-10.1 micrograms and 0.53+/-0.46-3.61+/-0.86 micrograms/hcm(2). Based on the results, lecithin vesicles of diclofenac appear to be advantageous for the topical delivery of diclofenac.     

CHOD—PAD法测定蛋黄卵磷脂中胆固醇的含量

目的:测量精制蛋黄卵磷脂中胆固醇的含量。方法:采用 CHOD-PAD 法。结果:胆固醇浓度在0.431-2.585mmol·L~(-1),r=0.9991,重复性实验 RSD 为2.6%,精密度实验 RSD 为0.42%,回收率为97.3%。检测限低于0.431mmol·L~(-1),可满足卵磷脂中胆固醇的检测需要。结论:方法操作简便,精密度好,结果准确可靠。