渭良伤科散超微粉巴布膏的抗炎镇痛实验研究

目的探讨渭良伤科散超微粉巴布膏的抗炎和镇痛作用。方法通过热板法和冰醋酸致痛试验、角叉菜胶致大鼠足趾肿胀试验、二甲苯致小鼠耳廓肿胀试验,对渭良伤科散超微粉巴布膏进行镇痛抗炎方面的药效学评价。结果渭良伤科散超微粉巴布膏中、高剂量的剂型可有效提高小鼠痛阈值,减少小鼠扭体反应次数,抑制角叉菜胶所致的大鼠足趾肿胀和抑制二甲苯所致的小鼠耳廓肿胀。结论渭良伤科散超微粉巴布膏中、高剂量的剂型有较好的镇痛抗炎作用,且在方便使用的基础上,优于原剂型渭良伤科散的镇痛抗炎作用。     

渭良伤科散超微粉巴布膏的抗炎镇痛实验研究

目的探讨渭良伤科散超微粉巴布膏的抗炎和镇痛作用。方法通过热板法和冰醋酸致痛试验、角叉菜胶致大鼠足趾肿胀试验、二甲苯致小鼠耳廓肿胀试验,对渭良伤科散超微粉巴布膏进行镇痛抗炎方面的药效学评价。结果渭良伤科散超微粉巴布膏中、高剂量的剂型可有效提高小鼠痛阈值,减少小鼠扭体反应次数,抑制角叉菜胶所致的大鼠足趾肿胀和抑制二甲苯所致的小鼠耳廓肿胀。结论渭良伤科散超微粉巴布膏中、高剂量的剂型有较好的镇痛抗炎作用,且在方便使用的基础上,优于原剂型渭良伤科散的镇痛抗炎作用。     

小茴香茎叶水提液的抗炎镇痛作用研究

目的:评价小茴香水提液的抗炎、镇痛作用.方法:应用二甲苯致小鼠耳廓肿胀研究小茴香水提液的抗炎作用,采用热板法、甲醛法、醋酸致小鼠扭体反应研究小茴香水提液的镇痛作用.结果:小茴香水提液提取物各剂量组与模型组比较,能降低二甲苯所致小鼠的耳廓肿胀度(P＜0.05);对热痛刺激的反应时间得到延缓(P＜0.05);对甲醛所致的疼...     

康妇炎胶囊抗炎镇痛的药效学研究

目的研究康妇炎胶囊的抗炎、镇痛作用。方法通过二甲苯致小鼠耳廓肿胀、醋酸致小鼠腹腔毛细血管通透性和琼脂致小鼠皮下肉芽肿实验观察康妇炎胶囊的抗炎作用,采用热板法和扭体法观察其镇痛作用。结果康妇炎胶囊能够显著抑制二甲苯所致小鼠耳廓肿胀,显著降低小鼠腹腔毛细血管通透性,抑制小鼠皮下肉芽肿的形成;并明显降低热刺激所致的小鼠痛阈值,减少小鼠的扭体次数。结论康妇炎胶囊对炎症有明显的抑制作用,且有一定的镇痛效果。     

消肿止痛液的药效学研究

目的研究消肿止痛液对实验动物炎性作用、镇痛作用以及活血化瘀作用的影响.方法分别以SD大鼠、小鼠为实验动物,观察消肿止痛液对角叉菜胶致大鼠足肿胀、二甲苯致小鼠耳廓炎症的作用,以及对镇痛、活血化瘀作用的影响.结果消肿止痛液局部涂擦能明显减轻角叉菜胶所致大鼠足肿胀和二甲苯致小鼠耳廓炎症：明显延长小鼠的痛阈值,减少小鼠扭体次数;显著促进小鼠瘀血吸收.结论消肿止痛液有消炎、镇痛和化瘀作用.     

大蒜素通过抗氧化应激缓解小鼠炎性痛的机制研究

目的探讨大蒜素抗炎性痛作用及抗氧化应激机制。方法采用冰醋酸致小鼠扭体、小鼠足底注射甲醛致痛、二甲苯致小鼠耳廓肿胀及角叉菜胶致小鼠足趾肿胀等实验,观察不同剂量大蒜素的抗炎性痛效应;采用生化法检测角叉菜胶致小鼠足趾肿胀组织SOD、MDA、GSH-Px及T-AOC含量变化。计量资料比较采用t检验,P<0.05为差异有统计学意义。结果与阴性对照组比较,大蒜素20 mg/kg组及40 mg/kg组小鼠扭体次数和累计舔足时间均降低;大蒜素40mg/kg组小鼠耳廓肿胀度和小鼠足趾肿胀度均低于阴性对照组,差异有统计学意义(均P<0.05)。与模型组比较,大蒜素40 mg/kg组小鼠足趾肿胀组织SOD、GSH-Px、T-AOC含量增加,差异有统计学意义(均P<0.05)。结论大蒜素对小鼠炎性痛有一定的缓解作用,其机制与抗氧化应激作用有关。     

舒胆浓缩丸的药效学研究

目的观察舒胆浓缩丸的主要药效学作用。方法采用巴豆油致小鼠耳廓肿胀法实验观察其抗炎作用;体内试验观察其抗菌作用;收集胆汁观察其利胆作用;及醋酸扭体法观察其镇痛作用。结果舒胆浓缩丸可明显降低小鼠耳廓,对体内绿脓杆菌有抑制作用,可明显增加小鼠胆汁流量,减少醋酸致小鼠扭体次数。结论舒胆浓缩丸具有抗炎、抑菌、利胆、镇痛作用。     

清热复方制剂用于急性咽炎治疗的药理实验研究

目的研究清热复方制剂对急性咽炎的抗炎、镇痛作用及对急性咽炎动物模型的影响。方法采用蛋清致大鼠足肿胀、二甲苯致小鼠耳肿胀、醋酸致小鼠扭体、热刺激致小鼠疼痛等实验方法研究清热复方制剂的抗炎及镇痛作用,采用25%氨水咽部喷雾方法建立大鼠急性咽炎模型,进行模型病理组织学检查。结果清热复方制剂能有效地抑制大鼠足肿胀度,延长小鼠疼痛反应时间,减少小鼠发生扭体的次数,同时阳性药对照组、清热复方制剂低、中、高剂量组咽部组织的粘膜上皮增生程度和炎细胞浸润程度与模型组比较均降低。结论清热复方制剂具有抗炎、镇痛作用,能缓解对氨水所致大鼠咽部粘膜上皮增生程度及炎细胞浸润程度,提高机体对急性咽炎的防治作用。     

温中安胃软胶囊药效学研究

目的:评价温中安胃软胶囊的主要药效.方法:采用热板法、扭体法观察镇痛作用,二甲苯致小鼠耳肿胀法及角叉菜胶致大鼠足趾肿胀法观察抗炎作用,小鼠大黄致泻法观察止泻作用,酚红排泄法观察对小鼠胃排空的影响,炭末推进法观察对小鼠肠推进的影响.结果:温中安胃软胶囊能提高小鼠热刺激痛阈,减少小鼠因冰醋酸致扭体反应数,对二甲苯致小鼠耳肿胀及角叉菜胶致大鼠足趾肿胀有抑制作用,减少大黄煎剂所致小鼠湿粪数,对正常及胃复安负荷小鼠胃排空有抑制作用,对阿托品负荷小鼠胃排空抑制亦有对抗作用,对正常小鼠及新斯的明负荷小鼠肠推进有抑制作用.结论:温中安胃软胶囊有抗炎、镇痛、止泻及调节胃肠运动作用.     

吡罗昔康新制剂微乳的药效学研究

目的：制备吡罗昔康微乳新剂型,对其抗炎、镇痛作用进行初步药效学研究,观察连续用药对皮肤组织形态学的改变,并与凝胶制剂进行比较。方法：采用油相Lauroglycol FCC,表面活性剂Labrasol、Cremophor EL,助表面活性剂TranscutolP、吡罗昔康及水制备吡罗昔康微乳,1％的双氯芬酸钠凝胶剂作为阳性对照药,采用二甲苯诱导小鼠耳廓肿胀,卡拉胶诱发大鼠足跖肿胀,痛阈提高率和醋酸致小鼠扭体实验,对吡罗昔康微乳的抗炎镇痛作用进行初步探讨并与市售的吡罗昔康凝胶进行比较。结果：吡罗昔康微乳具有抗炎镇痛作用,中、低剂量的吡罗昔康微乳与阳性对照组比较均显示相近或更强的抗炎镇痛作用,与吡罗昔康凝胶组比较,作用强、时间长、效果平稳,且对皮肤无刺激性。结论：吡罗昔康新制剂微乳安全,抗炎镇痛作用明显,有望成为吡罗昔康的新型制剂。     

沐浴藏盐对动物抗炎、镇痛作用的初步探讨

[目的]研究沐浴藏盐对动物的抗炎、镇痛作用,便于将其开发利用,以达到强身健体,特别是用于妇女病的防治和治疗。[方法]抗炎作用用皮肤毛细血管通透性亢进法和腹腔毛细血管通透性亢进法。镇痛作用用扭体法和电击法。[结果]毛细血管通透性亢进试验,以皮肤兰斑色泽深浅比较,3个试验组数值明显低于生理盐水对照组,表明本品有明显抑制小鼠腹部毛细血管通透性亢进作用;小鼠腹腔毛细血管通透试验结果,各试验组腹腔洗涤液兰色均比对照组浅,数值均低于对照组。表明“藏浴宝”有明显抑制H^＋所致小鼠腹腔毛细血管通透性亢进作用;扭体试验、电击试验、祛风镇痛百分率分别为65%、60%。[结论]“藏浴宝”有较好的抗炎、祛风镇痛作用。     

乳剂与膏剂消炎效果的比较

目的：研究乳剂与膏剂消炎效果方法：将50只雄性小白鼠随机分为正常对照组、石灰搽剂对照组、薄荷油乳剂组、风油精乳剂组、红霉素软膏组。正常对照组耳部涂抹生理盐水,阳性对照组耳部涂抹石灰搽剂,薄荷油乳剂组涂抹薄荷油乳剂,风油精乳剂组涂抹风油精乳剂,红霉素软膏组涂抹风油精乳剂。以抑制率和肿胀率为指标区分消炎效果。结果：阳性对照组、薄荷油乳剂组、风油精乳剂组、红霉素软膏组较正常对照组肿胀率和抑制率均有降低,乳剂组的消炎效果比膏剂组的消炎效果更明显。     

Research on heterocyclic compounds, XLI. 2-Phenylimidazo

The synthesis of a group of 2-phenylimidazo[1,2-b]pyridazine-3-acetic esters and acids is described. The structures of the new compounds are supported by 1H-NMR spectra. These compounds were tested in vivo for their anti-inflammatory, analgesic and ulcerogenic activity. All new compounds showed remarkable anti-inflammatory action in the carrageenan rat paw oedema (one third of that for indomethacin) but no significant analgesic activity in the acetic acid writhing test together with negligible ulcerogenic action, and were also found to be lacking inhibitory activity on cyclooxygenase in vitro.     

An investigation of the analgesic and anti-inflammatory effects of Nigella sativa seed polyphenols

Extracts obtained from the seeds of Nigella sativa are used as a spice or remedy for the treatment of various inflammatory diseases. The purpose of this study was to examine the analgesic and anti-inflammatory effects of its polyphenols. N. sativa seed polyphenols were prepared, and analgesic and anti-inflammatory effects were studied in mice and rats using the acetic acid-induced writhing, formalin, light tail flick, carrageenan-induced paw edema, and croton oil-induced ear edema tests. In the acetic acid-induced writhing test, oral administration of N. sativa polyphenols (NSP) decreased the number of abdominal constrictions. Both oral and intraperitoneal administration of NSP significantly suppressed in a dose-dependent manner the nociceptive response in the early and late phases of the formalin test, and the effect on the late phase was more pronounced. Pretreatment with naloxone failed to reverse the analgesic activity of NSP in this test. NSP did not produce a significant analgesia in the light tail flick test in mice. Oral administration of NSP did not produce a significant reduction in carrageenan-induced paw edema. However, when injected intraperitoneally, NSP inhibited paw edema in a dosedependent manner. NSP when applied topically failed to reduce croton oil-induced ear edema. These results suggest that NSP have analgesic and anti-inflammatory effects. The lack of analgesic effect of NSP in the light tail flick test and also the failure of naloxone to reverse the analgesia in the formalin test reveal that mechanisms other than stimulation of opioid receptors are involved.     

Synthesis, analgesic and anti-inflammatory activities of novel 3-(4-acetamido-benzyl)-5-substituted-1,2,4-oxadiazoles

A series of 3-(4-acetamido-benzyl)-5-substituted-1,2,4-oxadiazoles (7a-7n) were synthesized and screened for analgesic and in vivo anti-inflammatory activities using acetic acid writhing in mice model and carrageenan-induced paw oedema method in mice, respectively. The analgesic activity of compounds 7i and 7m is superior while that of 7d, 7c, 7f and 7j is equal to the reference standard, diclofenac sodium. The anti-inflammatory activity of compounds 6, 7c, 7e, 7f, 7i, 7l, 7m and 7n is found to be superior than that of diclofenac sodium which is used as a reference, while compounds 7d and 7g are found to be equipotent with the reference compound.     

Synthesis and anti-inflammatory, analgesic, ulcerogenic and lipid peroxidation activities of some new 2-[(2,6-dichloroanilino) phenyl]acetic acid derivatives

The synthesis of a group of 1,3,4-oxadiazoles, 1,2,4-triazoles, 1,3,4-thiadiazoles and 1,2,4-triazine derived from 2-[(2,6-dichloroanilino) phenyl] acetic acid is described. The structures of new compounds are supported by IR, (1)H-NMR and Mass spectral data. These compounds were tested in vivo for their anti-inflammatory activity. The compounds, which showed activity comparable to the standard drug diclofenac, were screened for their analgesic, ulcerogenic and lipid peroxidation activities. Ten new compounds, out of 28 showed very good anti-inflammatory activity in the carrageenin induced rat paw edema test, with significant analgesic activity in the acetic acid induced writhing test together with negligible ulcerogenic action. The compounds, which showed less ulcerogenic action, also showed reduced malondialdehyde content (MDA), which is one of the byproduct of lipid peroxidation. The study showed that the compounds inhibited the induction of gastric mucosal lesions and it can be suggested from our results that their protective effects may be related to inhibition of lipid peroxidation in the gastric mucosa.     

An investigation of the anti-inflammatory and analgesic effects of Orthosiphon stamineus leaf extract

Anti-inflammatory and analgesic activities of a standardized Orthosiphon stamineus methanol:water (50:50 vol/vol) leaf extract (SEOS) were evaluated in animal models. Oral administration of SEOS at doses of 500 and 1,000 mg/kg significantly reduced the hind paw edema in rats at 3 and 5 hours after carrageenan administration (P < .01 and P < .01; P < .01 and P < .05, respectively). SEOS (1,000 mg/kg, p.o.) also produced significant (P < .05) analgesic activity in both the acetic acid-induced writhing test and the formalin-induced licking test (late phase) in mice and rats, respectively. However, SEOS showed no effect on the tail flick and hot plate tests in mice. The results of the present study support the proposal that O. stamineus has anti-inflammatory and non-narcotic analgesic activities. These findings justify the traditional use of the plant for treating pain and inflammation.