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1.
目的:利用TLC和HPLC对解热抗炎Ⅰ号口服液中有效成分大黄素进行定性、定量分析。方法:用TLC法对成分大黄素和薄荷进行定性分析,以HPLC法在ODS-C18柱上以甲醇-0.1%高氯酸(20:80)为流动相测定其含量。结果:大黄素回收率为96.89%(RSD=0.96%),检测范围为0.05~0.8μg/mL,r=0.999 5,可以作为该制剂的质控方法。结论:所用定性、定量方法快速简便、灵敏度高  相似文献   

2.
采用TLC法对板蓝根药材中氨基酸进行了定性鉴别,并应用TLCS法测定了药材板蓝根中靛玉红的含量,并 用常量法对其合氮量进行了测定,结果表明药材中靛玉红含量大都在8μg/g左右,合氮量都在0.6%以上。  相似文献   

3.
TLC法测定何首乌及其制品中大黄素的含量   总被引:3,自引:0,他引:3  
TLC法测定何首乌及其制品中大黄素的含量罗健黎知林李娟钟爱(广东药学院药物研究所,广州510224)关键词何首乌;大黄素;薄层扫描法中图号R927.2何首乌又称生首乌,而制首乌、首乌补汁及百消丹均是含何首乌的药品。何首乌为防老抗衰要药,具有消炎,散...  相似文献   

4.
目的:测定健延龄胶囊中大黄素、大黄素甲醚的含量。方法:采用薄层扫描法。结果:10批样品中大黄素平均含量为1.993‰,平均加样回收率99.08%,RSD为3.094%;大黄素甲醚平均含量为0.845‰,平均加样回收率99.12%,RSD为2.818%。结论:该方法为健延龄胶囊的质量控制提供了依据。  相似文献   

5.
目的:研究消坚止血颗粒的定性、定量方法及制剂常规标准。方法采用TLC法鉴别处方主要药味,采用比色法对制剂中总蒽醌、总黄酮定量分析,并进行制剂常规检测。结果TLC法显示,益母草、仙鹤草、川牛膝、莪术等样品色谱与对照品色谱在相应位置上显现相同斑点;样品中总蒽醌和总黄酮含量分别不低于0.5mg/g和15mg/g。结论定性、定量方法简便易行,结果可靠,可作为该制剂的质量控制标准。  相似文献   

6.
平脂胶囊对鼠血脂和血粘的影响   总被引:4,自引:0,他引:4  
王四旺  谢艳华  朱玲珍 《医学争鸣》1999,20(10):851-854
目的:观察平脂胶囊对动物血脂和血粘等指标的影响。方法:采用高血脂模型,按照酶学、组织化学及直接测压等方法测定血脂、组织中脂质、血液流变、血压等指标。结果:(1)0.4g/kg,0.8g/kg平脂胶囊使高血脂小鼠体质量、肝脏系数、肝组织胆固醇(TC)和血清TC显著降低;(2)对高血压-高血脂大鼠体质量和心脏、主动脉、肝脏系数;血清TC、甘油三脂(TG)、主动脉、心肌、肝脏组织中TC、TG以及全血粘度  相似文献   

7.
采用TLC法对肝丸中的白芍,五味子进行定性鉴别,并应用TLCS测定肝丹丸中芍药甙的含量,结果表明,样品中芍药甙的含量为0.118%,平均回收率为98.02%,RSD为在1.45%。  相似文献   

8.
研究平骨胶囊的质量标准。方法:采用TLC法分离平骨胶囊中士的宁、马钱子碱和麻黄碱,并采用薄层扫描法和薄层酸性染料比色法逐一对其含量进行测定。结果:平骨胶囊中士的宁含量为0.0994%,马钱子碱含量为0.0289%,麻黄碱含量为0.2488%。结论:本法可作为该制剂质量控制方法。  相似文献   

9.
为探索参柏液的鉴别及含量测定方法,给制订其质量标准提供可靠依据,作者采用TLC法对参柏液中的苦参、黄柏及苍术等中药进行了鉴定。该品中君药苦参的测定,以中性氧化铝柱固相净化分离样品,用氯仿洗脱苦参生物碱,洗脱液用硫酸液(0.01mol/L)提取后,以氢氧化钠液(0.02mol/L)滴定,甲红为指标剂,按苦参碱(C15H24N2O)计算本品中苦参生物碱量。该法可避免样品中其他组分及色素的干扰,滴定终点敏锐,RSD%为1.10(n=5),加样回收率为97.59±1.31%(n=8)。用以测定10批样品,均获得满意结果。  相似文献   

10.
为探索参柏液的鉴别及含量测定方法,给制订其质量标准提供可靠依据,作者采用TLC法对参柏液中的苦参、黄柏及苍术等中药进行鉴定。该品中君药苦参的测定,以中性氧化铝柱固相净化分离样品,用氯仿洗脱苦参生物碱,洗脱液用硫酸液(0.01mol/L)提取后,以氢氧化钠液(0.02mol/L)滴定,甲红为指标剂,按苦参碱(C15H24N2O)计算本品中苦参生物碱量。该法可避免样品中其他组分化及色素的干扰,滴定终点  相似文献   

11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

12.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

13.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

14.
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.  相似文献   

15.
Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.  相似文献   

16.
Objective:To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas(SPTP). Methods:Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin(HE) staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results:Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin(9/9 cases), AAT(9/9 cases), NSE(9/9 cases), ACT(7/9 cases), CK20(2/9 cases), CgA(1/9 cases), S-100(3/gcases), PR(4/gcases), Syn(3/9 cases) and CD56(5/9cases), negative in CEA and ER. Conclusion:SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.  相似文献   

17.
In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.  相似文献   

18.
Objective: To explore the role of matrix metalloproteinase-1,2 (MMP-1, MMP-2) and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) in endometriosis. Methods: The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects (excluding endometriosis) were collected and examined by in situ hybridization technology and western blot assay. Results: Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant (P 〈 0.01 ). Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion: The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.  相似文献   

19.
Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.  相似文献   

20.
《中国结合医学杂志》2008,14(2):159-159
The 6th National General Congress of Chinese Association of Integrative Medicine (CALM) was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.  相似文献   

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