血管内皮生长因子基因在正常造血细胞中的表达

目的 探讨正常造血细胞中血管内皮生长因子（ＶＥＧＦ）基因的表达水平。方法 ＴＲＩｚｏｌ一步法提取正常外周血和非恶性骨髓标本单个核细胞及正常血小板的总ＲＮＡ;以β２－微球蛋白基因为内对照,逆转录聚合酶链反应（ＲＴ－ＰＣＲ）半定量分析ＶＥＧＦ基因的表达。结果 ２２分外周血标本有１８份表达ＶＥＧＦ基因,表达率为８１．８％,其中８份为中度表达;１８份骨髓标本ＶＥＧＦ基因的表达率高达９４．４％（１７／１８）,其中高度表达和中度表达分别为５份与６份;血小板有ＶＥＧＦ基因的低度表达。结论正常造血细胞要表达ＶＥＧＦ基因,提示体内血管生成受到多种效应细胞的共同调节。     

骨髓增生异常综合征骨髓病态造血细胞的克隆性分析

目的探讨骨髓增生异常综合征(MDS)骨髓中三系病态造血细胞的克隆性。方法联合应用DNA荧光原位杂交(FISH)、碱性磷酸酶-抗碱性磷酸酶系统(APAAP)免疫细胞化学法以及免疫磁珠分选技术调查MDS骨髓中三系病态造血细胞的克隆性。结果21位患者的标本中共检出184个病态粒细胞,其中140个(76%)携带正常的克隆信号。21份标本中仅38个三核红细胞和25个核出芽红细胞被检出,其中32个(85%)和19个(76%)显示正常核型。14例患者骨髓标本中共检出265个淋巴细胞样微小巨核细胞,其中170个(64%)为正常核型。三系病态造血细胞中的非克隆细胞百分比均超过了这些患者骨髓所有有核中平均非克隆细胞百分比(47%)。结论MDS骨髓中的病态造血细胞并非镜像地象征恶性克隆细胞,而是残存正常造血细胞的形态学变化。     

骨髓增生异常综合征骨髓病态造血细胞的克隆性分析

目的 探讨骨髓增生异常综合征(MDS)骨髓中三系病态造血细胞的克隆性.方法 联合应用DNA荧光原位杂交(FISH)、碱性磷酸酶-抗碱性磷酸酶系统(APAAP)免疫细胞化学法以及免疫磁珠分选技术调查MDS骨髓中三系病态造血细胞的克隆性.结果 21位患者的标本中共检出184个病态粒细胞,其中140个(76%)携带正常的克隆信号.21份标本中仅38个三核红细胞和25个核出芽红细胞被检出,其中32个(85%)和19个(76%)显示正常核型.14例患者骨髓标本中共检出265个淋巴细胞样微小巨核细胞,其中170个(64%)为正常核型.三系病态造血细胞中的非克隆细胞百分比均超过了这些患者骨髓所有有核中平均非克隆细胞百分比(47%).结论 MDS骨髓中的病态造血细胞并非镜像地象征恶性克隆细胞,而是残存正常造血细胞的形态学变化.     

全反式维甲酸对急淋白血病骨髓基质细胞粘附能力的影响

[目的]探讨全反式维甲酸(ATRA)对急性淋巴细胞自血病(ALL)骨髓基质细胞(BMSC)粘附能力的影响.[方法]将ATRA加进骨髓基质细胞培养体系,培养18 h后用流式细胞仪检测6例ALL骨髓基质细胞表达粘附分子ICAM-1和VCAM-1阳性率,用MTT方法检测ALL骨髓基质细胞对正常骨髓造血细胞或淋巴瘤Raji细胞的粘附率.[结果]药理浓度的ATRA(1.0 μmol/L)使ALL骨髓基质细胞ICAM-1表达明显增高(P<0.05),使ALL骨髓基质细胞对正常骨髓造血细胞或肿瘤细胞的粘附率均明显增高(P<0.05).[结论]药理浓度的ATRA可增强ALL骨髓基质细胞对正常造血细胞和肿瘤细胞的粘附能力.     

HIF-1α、VEGF表达与脑胶质瘤预后的相关性

目的　探讨缺氧诱导因子 - 1α(HIF - 1α)、血管内皮生长因子 (VEGF)表达对人脑胶质瘤预后的影响。方法　采用免疫组织化学方法检测 6 3例脑胶质瘤HIF - 1α、VEGF的表达状况 ,并将结果与临床预后随访结果进行综合分析。结果　HIF - 1α总阳性表达率为 71.4 % ,其中强表达 16例 (2 5 .4 % ) ,中度表达 14例 (2 2 .2 % ) ,弱表达 15例 (2 5 .4 % ) ,不表达 18例 (2 8.6 % ) ;与正常对照组比较有显著性差异 (P <0 .0 1)。HIF - 1α表达水平与胶质瘤恶性程度相关 (r =0 .4 6 7,P <0 .0 1)。HIF - 1α主要表达于肿瘤边缘或与正常组织交界处的细胞及坏死的肿瘤组织中。不同组织学分级的脑胶质瘤标本肿瘤细胞质均有VEGF表达 ,胶质瘤Ⅰ级、Ⅱ级、Ⅲ～Ⅳ级VEGF阳性细胞数分别为 175± 6 6、35 2± 10 0、6 6 2± 12 3(个 ) ,3组比较有显著性差异 (P <0 .0 1)。VEGF与HIF - 1α表达水平有关 (r=0 .817,P <0 .0 1)。患者生存时间与HIF - 1α呈负相关 (r =- 0 .82 4 ,P <0 .0 1)。结论　VEGF与HIF - 1α表达有一定的相关性 ,HIF - 1α与脑胶质瘤新生血管生成有关。HIF - 1α、VEGF联合检测可更准确判断患者的预后。     

肾细胞癌组织与正常组织中VEGF165b的差异表达及其意义

目的:研究血管内皮生长因子165b(Vascular endothelial growth factor 165b,VEGF165b)在肾细胞癌(Renal cell carcinoma,RCC)组织和正常组织中的表达情况,初步探讨其与肾细胞癌发生发展的关系.方法:(1)用S-P免疫组化法检测30例肾细胞癌组织和29例正常肾组织中VEGF165b蛋白的表达情况;(2)用RT-PCR法分别检测32例肾细胞癌组织和30例正常肾组织中VEGF165b mRNA的表达情况.结果:(1)正常肾组织中VEGF165b蛋白表达率为96.55%(28/29),肾细胞癌组织中VEGF165b蛋白的表达率为20.00%(6/30),表达差异有统计学意义(P<0.05). (2)正常肾组织中VEGF165b mRNA阳性表达率为93.33%(28/30),肾细胞癌组织中VEGF165b mRNA的阳性表达率为18.75%(6/32),具有统计学意义(P相似文献   

IVF-ET术后流产患者绒毛和蜕膜组织凋亡及相关调控蛋白的表达

目的:检测体外受精-胚胎移植(IVF-ET)术后早期自然流产患者绒毛和蜕膜组织凋亡及相关调控蛋白血管内皮生长因子(VEGF)、Bcl-2的表达,探讨其发病和基因调控机制。方法:采用免疫组织化学染色技术检测IVF-ET术后早期自然流产者(IVF-ET组)绒毛及蜕膜组织Bcl-2、VEGF的表达水平,并以同期正常早孕者(正常早孕组)的标本为对照。结果:VEGF主要表达于绒毛滋养细胞和血管内皮细胞胞浆。IVF-ET组VEGF表达明显低于正常早孕组,差异具有统计学意义(P<0.05)。Bcl-2主要在绒毛合体滋养细胞上表达。IVF-ET组滋养细胞及蜕膜细胞染色较浅,表达呈弱阳性,正常早孕组染色较深,表达强阳性,差异有统计学意义(P相似文献   

鼻息肉组织中肝细胞生长因子及血管内皮生长因子的表达

目的:检测鼻息肉组织中肝细胞生长因子(HGF)及血管内皮生长因子(VEGF)的表达.方法:采用免疫组织化学方法分别检测30例鼻息肉标本和20例正常下鼻甲黏膜标本中HGF与VEGF的表达.结果:鼻息肉组织中可见大量嗜酸性粒细胞浸润.鼻息肉组织中HGF和VEGF蛋白的阳性表达率分别为83.3%和76.7%,均高于正常对照组(30.0%和10.0%,P<0.05),且在鼻息肉血管内皮细胞及间质浸润的炎症细胞中2者的表达呈正相关(r=0.630,P<0.05;r=0.514,P<0.05).结论:鼻息肉组织中HGF和VEGF的表达增强,提示2者可能协同参与鼻息肉的形成.     

VEGF基因在兔骨髓基质细胞中的表达及活性测定

目的探讨人血管内皮细胞生长因子(VEGF)基因修饰的兔骨髓基质细胞对VEGF基因的表达及表达产物的生物学活性.方法制备真核表达质粒pAdCMV-VEGF165,以Superfect转染试剂介导VEGF基因转染兔骨髓基质细胞,收获转染后24h至6d的转染上清和细胞,采用RT-PCR技术、免疫组化SABC法检测转染细胞中外源性VEGF165基因的转录及表达,用血管内皮细胞增殖试验测定转染细胞培养上清中VEGF的生物活性.结果VEGF基因转染的兔骨髓基质细胞可有效转录VEGF165,其分泌于培养上清中的表达产物可促进血管内皮细胞增殖,具有很强的生物活性.结论VEGF基因转染的兔骨髓基质细胞可有效表达具有生物活性的VEGF.     

妊娠肝内胆汁淤积症患者胎盘组织中血管内皮生长因子的表达

目的观察血管内皮生长因子(VEGF)在妊娠肝内胆汁淤积症(ICP)胎盘中的表达.方法采用免疫组织化学方法,检测25例正常妊娠胎盘(对照组)和25例ICP胎盘(ICP组)中VEGF的水平.结果 VEGF在正常妊娠胎盘和ICP胎盘中分布基本一致,分布在滋养细胞、血管及绒毛间质.在ICP组VEGF为轻度表达占76%,中度表达占24%,无重度表达;而在对照组VEGF轻度表达占24%,中度表达占44%,重度表达占32%.ICP组中VEGF表达明显低于对照组(P＜0.01).结论 VEGF在胎盘中主要由绒毛滋养细胞分泌,ICP胎盘中VEGF的减少可能与胎盘血管生成减少及胎盘滋养叶细胞浸入异常有关,在ICP发病中占有一定的地位.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.