健康成年人半乳糖凝集素-3参考范围调查

目的调查健康人群血清半乳糖凝集素-3参考范围。方法用ELISA法检测1 173名(男585名,女588名)健康成年人半乳糖凝集素-3水平。结果半乳糖凝集素-3参考范围:男性(21~30)岁:(3.90±1.86)μg/L;(31~40)岁:(4.33±1.95)μg/L;(41々50)岁:(4.68±1.89)μg/L;(51~70)岁:(5.05±2.21)μg/L;(71~80)岁:(5.23±2.46)μg/L。女性(21~30)岁:(3.51±1.71)μg/L;(31~40)岁:(4.01±1.86)μg/L;(41~50)岁:(4.34±1.89)μg/L;(51~70)岁:(4.73±2.01)μg/L;(71~80)岁:(4.96±2.35)g/L。男女间差异有统计学意义(P0.05)。男、女性血清半乳糖凝集素-3水平均随年龄增长呈现升高的趋势。结论健康成年人群血清半乳糖凝集素-3参考范围存在性别、年龄的差异,建议各实验室根据性别、年龄建立各自的参考范围。     

2020年许昌市健康人群白喉抗体水平调查

目的了解2020年许昌市健康人群白喉抗体水平。方法采用分层随机抽样方法选取0-79岁健康人群,采用酶联免疫吸附试验检测血清白喉IgG抗体,分析抗体阳性率和几何平均浓度(Geometric mean concentration, GMC)。结果 2020年许昌市健康人群白喉IgG抗体总阳性率为75.80%(761/1 004),GMC为0.03IU/mL;各年龄组白喉IgG抗体阳性率在39.39%(45-59岁)-98.44%(1-2岁)之间(χ~2=267.73,P0.001),GMC在0.003IU/mL (45-59岁)-0.13 IU/mL(1岁和1-2岁)之间(F=73.25,P0.001);在0-14岁儿童中,有、无/不详白喉免疫史者抗体阳性率分别为96.53%(473/490)、75.00%(12/16)(χ~2=18.06,P0.001),GMC分别为0.10、0.04IU/mL(t=9.98,P=0.06)。结论许昌市健康人群白喉抗体水平和阳性率随着年龄增长呈下降趋势;建议开展青少年或成人白喉加强免疫。     

探讨建立江苏地区健康人群血清铁蛋白参考区间

目的调查分析江苏地区健康人群血清铁蛋白水平,探讨江苏地区人群铁蛋白参考区间。 方法回顾性分析24 803例江苏地区2017年1月至12月间送检到我中心使用化学发光免疫分析法检测的体检人群血清铁蛋白结果。将统计人群按照性别、年龄分组,使用SPSS软件进行统计分析,比较各分组的平均铁蛋白水平。 结果江苏地区健康成人血清铁蛋白水平呈偏态分布,不同性别、不同年龄组间血清铁蛋白水平有差异,经统计得出的的男性血清铁蛋白参考区间为：18~30岁(8.30~373.02 ng/ml)、31~40岁(11.88~481.60 ng/ml)、41~50岁(19.34~497.34 ng/ml)、51~65岁(15.05~495.41 ng/ml)、大于65岁(16.01~511.48 ng/ml),其中18~30岁及30岁以上人群组铁蛋白水平有统计学差异;女性血清铁蛋白参考区间为：18~30岁(7.44~181.76 ng/ml)、31~40岁(5.51~193.91 ng/ml)、41~50岁(4.33~218.88 ng/ml)、51~65岁(7.23~404.37 ng/ml)、大于65岁(7.36~449.67 ng/ml),其中18~50岁及50岁以上人群组铁蛋白水平有统计学差异。 结论影响铁蛋白浓度的原因除了性别外,铁蛋白水平随年龄递增呈现升高趋势,各地区实验室应建立不同性别及年龄区间的铁蛋白参考区间,从而为临床诊断提供可靠的检测数据。     

上海市松江区孕妇麻疹和风疹抗体水平监测

目的了解上海市松江区孕妇麻疹和风疹IgG抗体水平,为预防初免前婴儿麻疹、风疹及新生儿先天性风疹综合征的发生提供科学的免疫策略。方法分别于2013年5—8月和2014年4—6月,按照就诊顺序用随机数字法随机抽取松江区妇幼保健院和第一人民医院产科15~岁、25~岁、35~岁3个年龄组600名健康孕妇进行监测,采用酶联免疫吸附试验(ELISA)检测每名孕妇血清的麻疹和风疹IgG抗体水平,采用SAS 9.2软件对相关数据进行统计学分析。结果 600名健康孕妇的麻疹IgG抗体几何平均浓度和阳性率分别为885.58 m IU/mL和86.50%,风疹IgG抗体几何平均浓度和阳性率分别为197.85 IU/mL和82.17%;非本市户籍孕妇的麻疹和风疹抗体水平均高于本市户籍孕妇,各年龄组间孕妇麻疹和风疹抗体水平差异无统计学意义(P均0.05)。结论为控制初免前婴儿麻疹、风疹的发病及新生儿先天性风疹综合征的发生,应在育龄期妇女中开展含麻疹和风疹成分疫苗的补种。     

血清杀菌试验和酶联免疫吸附试验测定C群脑膜炎奈瑟菌血清抗体滴度比较

目的 比较血清杀菌试验(SBA)和酶联免疫吸附试验(ELISA)测定两种C群脑膜炎奈瑟菌(Nm)疫苗免疫后血清抗体滴度的差异.方法 采用SBA测定75名未免疫健康成年人(40～70岁)血清、143名3～8月龄婴儿及194名3～5岁儿童NmA和C群结合疫苗或A+C群多糖疫苗免疫前后血清中Nm杀菌抗体水平,然后用ELISA测定相应血清的Nm特异性IgG含量,并利用线性相关与回归分析两种测定结果的相关性.结果 未免疫健康成年人血清中Nm杀菌抗体水平和特异性IgG含量之间的相关性较高(r=0.814 33,P＜0.001);3～8月龄婴儿和3～5岁儿童接种结合/多糖疫苗前,Nm杀菌抗体水平和特异性IgG含量之间相关性较差(3～8月龄:r=0.140 64,P＞0.l00/r=0.2899,P＜0.05;3 ～5岁:r=0.540 40,P＜0.05/r=0194 36,P＜0.05),接种1剂结合疫苗后,杀菌抗体水平和特异性IgG含量之间的相关性较好(r=0.809 38,P＜0.001和r=0.837 23,P＜0.001);接种多糖疫苗后,杀菌抗体水平和特异性IgG含量之间的相关性较差(r＜0.500 00).结论 健康成年人血清和结合疫苗免疫后的婴幼儿血清中特异性C群Nm IgG含量可以间接反映血清杀菌抗体水平,ELISA方法可以替代SBA.但ELISA检测方法不适用于3～8月龄婴儿多糖疫苗免疫后的效果评价.     

江苏省2018年健康人群破伤风IgG抗体水平调查北大核心

目的了解江苏省2018年健康人群破伤风IgG抗体水平。方法采用分层抽样选择江苏省3个区县9个年龄组健康人群,采用酶联免疫吸附试验检测血清破伤风IgG抗体,分析抗体阳性率和几何平均浓度(GMC)。结果江苏省2018年健康人群破伤风IgG抗体总阳性率为68.67%(618/900),抗体GMC为0.071IU/mL。年龄别抗体阳性率在7.00%(30-39岁)-100%(0-2岁)之间(χ^2=610.26,P<0.001);0-14岁、≥30岁人群抗体阳性率分别为97.40%(487/500)、8.50%(17/200)。结论江苏省健康人群破伤风抗体水平随着年龄的增长而降低;建议开展成人尤其是育龄期妇女破伤风疫苗补充免疫。     

长沙地区健康孕妇肾功能参考区间的建立

目的初步建立长沙地区健康妊娠期妇女肾功能参考区间,为指导长沙地区孕妇临床观察提供理论依据。方法采用贝克曼库尔特AU5800全自动生化分析仪检测长沙地区2 083例健康孕妇血清肾功能三项(尿素、肌酐、尿酸),使用SPSS19.0统计软件进行分析,得出其参考区间,并比较不同年龄和不同孕周期组之间的差异。结果健康孕妇的尿素(1.79~5.31 vs 2.90~7.14)和肌酐(42.8~81.9 vs 53.0~132.6)的参考区间均低于健康成人,而尿酸(159.3~433.7 vs142.0~416.0)则高于健康成人。健康妊娠期妇女的尿素和尿酸参考区间与年龄无关,而肌酐随着年龄的增长参考区间数值稍降低;不同孕周期的健康孕妇肾功能差异有统计学意义,尿素和肌酐的参考区间数值变化趋势相同(孕晚期孕早期孕中期),而尿酸的变化趋势不同(孕晚期孕中期孕早期)。结论应建立符合健康妊娠期妇女不同年龄和不同孕周期的肾功能参考区间,对判断孕妇肾功能改变有重大意义。     

广州市正常儿童血清维生素B1和B2参考区间调查

目的 建立广州市健康儿童血清维生素B1和B2的参考区间,为临床精准判断儿童维生素B1、B2状态提供循证医学证据。方法 应用LK3000V维生素检测仪对广州市1 442名健康儿童(男性809例,女性633例)的血清维生素B1和B2进行检测,对结果进行统计分析。结果 1)10岁以内的10个年龄组、10~17岁和>17岁组血清维生素B1和B2水平性别差异均无统计学意义(P>0.05)。 2)血清维生素B1水平[中位数(P2.5,P97.5)]为:0~3岁组为41.9(45.6,88.1 nmol/L,单位下同),3~5岁组为65.5(45.6,98.4),5~17岁组为72.9(46.3,105.8),>17岁组为80.6(46.7,115.9)。血清维生素B2的水平为:0~5岁组为285.6(197.3, 376.2 μg/L,下同),5~17岁组为297.9(208,387.4),>17岁组为308.2(280.6,394.7)。3)年龄与血清维生素B1和B2相关系数分别是0.345和0.226(P<0.05)。结论 广州市正常儿童血清维生素B1参考区间无年龄差异,而血清维生素B2参考区间以5岁为界分别建立。     

长沙市区血清总蛋白、白蛋白参考区间的建立

目的在本实验室建立适合本地区人群的总蛋白(TP)、白蛋白(ALB)生物参考区间。方法收集4740例长沙城区健康参考个体测定TP和ALB值,与本实验室原有参考区间进行验证,对于验证不通过的项目,再建立适用于本实验室的生物参考区间,定期进行临床评审。结果 TP、ALB现用生物参考区间验证不通过,不适用,须自建生物参考区间,自建参考区间为TP:68.2～85.0g/L;ALB:42.2～54.3g/L。同时数据显示不同年龄段健康人群TP和ALB值有一定差异,随着年龄增长有降低趋势;且与季节变换有一定相关性,呈现冬季高于夏季的变化趋势。结论长沙市区健康人群血清蛋白质水平与原参考值差异有统计学意义,原参考值已不能很好的用来评价人群的健康水平和给临床提供准确的诊断依据,应重新确定可靠、准确的生物参考区间。     

杭州市2018年0-59岁健康人群W135群脑膜炎奈瑟菌IgG抗体水平调查

目的评估杭州市健康人群W135群脑膜炎奈瑟菌(Neisseria meningitidis,Nm)抗体水平。方法采用年龄分层随机抽样于2018年10月选择杭州市0-59岁(9个年龄组)健康人群,采集血清标本;采用酶联免疫吸附试验检测W135群Nm IgG抗体,分析抗体阳性率和几何平均浓度(Geometric mean concentration, GMC)。结果调查对象W135群Nm IgG抗体总阳性率、GMC分别为28.43%(85/299)、1.23μg/mL。各年龄组W135群Nm IgG抗体阳性率在0.00%(30-39岁)-72.73%(3-4岁)之间(χ~2=73.14,P0.001);含W135群Nm成分疫苗0剂次、1剂次、2剂次、不详免疫史人群的W135群Nm IgG抗体阳性率分别为27.97%、72.73%、66.67%、17.93%(χ~2=38.17,P0.001)。结论杭州市0-59岁健康人群W135群Nm IgG抗体水平偏低,人群易感性高。     

2011年黔东南州390名8月龄至6岁健康儿童麻疹血清抗体水平调查

目的了解黔东南州8月龄至6岁健康儿童麻疹抗体水平,为控制麻疹提供科学依据。方法2011年6～7月,在黔东南州抽取锦屏县、剑河县和凯里市的390名8月龄至6岁健康儿童进行血清麻疹IgG抗体水平检测。结果检测390人,麻疹IgG抗体阳性的349人,阳性率为89．49％;抗体几何平均滴度为1：890．54,其中滴度≥1：800（达到保护水平）者253人,占64．87％。各年龄组血清麻疹抗体阳性率均在85％以上,达到保护水平者所占比例均在59％以上。麻疹抗体阳性率,接种麻疹疫苗1针次的为84．31％,接种2针次的为92．83％（P〈O．01）;男性为89．54％,女性为89．40％（P〉0．05）;凯里市为87．74％,剑河县为87．50％,锦屏县为92．81％（P〉0．05）;汉族为88．00％,苗族为89．15％,侗族为91．43％（P〉0．05）。结论黔东南州8月龄至6岁健康儿童麻疹抗体水平不高。     

Correlation of Anti-SARS-CoV-2 S1-specific IgG antibody levels and adverse events following vaccination with BNT162b2 mRNA COVID-19 vaccine in healthcare workers

Currently approved anti-COVID-19 vaccines have been found to be safe and effective and almost 60% of Israeli residents are already vaccinated with BNT162b2 vaccine.This observational study was designed to evaluate the adverse events of vaccine reported by 61 healthcare workers at least 7 days after the 2nd vaccination, and to investigate the correlation of adverse events and anti-SARS-CoV-2 IgG antibody levels.The median participant's age was 51.25 years, 16 men and 45 women; 77% (44% of male and 84.5% of female participants) reported adverse events. Injection site pain, fatigue and fever were the most common symptoms, and significantly higher antibody levels (average 19,387 AU/mL) were found in participants who had fever compared to those who did not experience fever (average antibody levels of 9,977 AU/mL, p < 0.001). This finding corresponds to previous observations of higher anti-SARS-CoV-2 IgG antibody levels in COVID-19 patients presented with fever.     

Reference interval of serum thyroid hormones in healthy Korean adults]

OBJECTIVES: This study was conducted to determine the reference interval of serum thyroid hormones (TSH, FT3, FT4) in healthy Korean adults. METHODS: Health examination data from 1,591 healthy Korean adults who visited an university hospital were analyzed. Patients with specific health conditions capable of altering laboratory results were excluded from the study. Serum thyroid hormones were measured using IMMULITE 2000 (DPC, USA, 2002). Subjects were 18-65 years old; 911 were male, and 690 were female. RESULTS: The arithmetic means of TSH, FT3, and FT4 values for male subjects were 1.28+/-1.84 microIU/ml, 3.23+/-0.57 pg/ml, and 1.42+/-0.22 ng/dl, respectively. In female subjects, the arithmetic means of TSH, FT3, and FT4 values were 1.49+/-2.08 microIU/ml, 3.08+/-0.54 pg/ml, and 1.29 +/-0.24 ng/dl, respectively. The arithmetic mean FT4 value for males decreased with age (p<0.01). The arithmetic mean FT3 value for females increased with age (p<0.01). The arithmetic mean thyroid hormone values of all study subjects differed significantly based on season. The arithmetic mean of male FT4 decreased with increasing BMI (p<0.01). The arithmetic mean of female FT3 increased with increasing BMI (p<0.01). The reference intervals recommended by the IMMULITE 2000 manufacturer are 0.40-4.00 microIU/ml for TSH, 1.80-4.20 pg/ml for FT3, and 0.80-1.90 ng/dl for FT4 (same values for both genders). CONCLUSIONS: There was a significant difference in the interval of thyroid hormones between males and females, but the reference interval of IMMULITE 2000 was not established by gender. There is a need to reestablish the reference interval for thyroid hormones in Korean healthy adults.     

2018年成都市健康人群破伤风抗体水平监测结果分析

目的分析成都市健康人群破伤风抗体水平情况,评价含破伤风类毒素疫苗免疫效果,为优化免疫策略提供依据。方法 2018年抽取成都市一、二、三圈层5个区(市)县,共调查1 105名健康人群人口和疫苗接种信息,采集血清标本进行破伤风IgG检测。采用χ~2检验和秩和检验对数据进行分析。结果健康人群总破伤风抗体浓度M为0.34 IU/ml,达到充分免疫或长期保护水平(>0.1 IU/ml)的率为68.60%。5个地区破伤风抗体浓度M在0.270 IU/ml^0.415 IU/ml之间(H=21.392,P<0.01),抗体浓度达到充分免疫或长期保护水平的率在63.96%~75.41%之间(χ~2=104.402,P<0.01);各年龄组破伤风抗体浓度M在0.030 IU/ml^0.955 IU/ml之间(H=599.017,P<0.01),抗体浓度达到充分免疫或长期保护水平的率在14.29%~95.83%之间(χ~2=690.965,P<0.001);男性破伤风抗体浓度(0.415 IU/ml)比女性(0.280 IU/ml)高(Z=-3.069,P=0.002)、男性(73.33%)抗体浓度达到充分免疫或长期保护水平的率也高于女性(64.96%)(χ~2=8.876,P=0.012)。结论需进一步加强中心城区流动儿童及郊区预防接种管理工作,建议在成年人群中引入第6剂次含破伤风类毒素疫苗的加强免疫。     

Sexual diergism in antibody response to whole virus trivalent inactivated influenza vaccine in outbred mice

An outbred mouse model was used to determine if antibody response to immunization with whole-virus trivalent inactivated influenza vaccine (TIV) differs between the sexes. The antibody response was examined one (serum titer of IgM antibodies), and three and six weeks post-immunization (serum titer of neutralizing and total IgG antibodies and IgG subclass profile). Compared with male in female mice was found (i) the more robust IgM response against all influenza strains included in TIV and (ii) more vigorous neutralizing antibody and total IgG responses against H1N1 influenza virus at both the examined time points post-immunization. The total IgG antibody response against H3N2 and B influenza viruses was comparable between female and male mice three weeks post-immunization, but significantly greater in female mice six weeks post-immunization. The neutralizing antibody response against H3N2 and B influenza viruses did not significantly differ between sexes at both the examined points post-immunization. Finally, three weeks post-immunization subclass profile of IgG specific to the influenza strains included in TIV differed between female and male mice, reflecting the lower titer of IgG1 antibodies in female ones, so that IgG2a (contributing mainly to the total IgG) to IgG1 ratio in mice of this sex was shifted toward the former. In agreement with this shift, compared with male mice, Th1/Th2 balance in female mice was shifted toward Th1, as shown by ELISPOT. Collectively, the results showed influenza virus strain-dependent sexual dimorphism in the magnitude, dynamics and characteristics of antibody response in outbred mice immunized with TIV.     

2014年贵州省健康人群麻疹IgG抗体监测结果分析

摘要:目的　了解贵州省部分县健康人群麻疹IgG抗体水平,为制定麻疹防控策略提供科学依据。方法　采集10县10个乡（镇）8个年龄组＜1岁～≥20岁人群血清标本2 552份,采用酶联免疫吸附法检测麻疹IgG抗体。结果　检测2 552份血标本,麻疹IgG抗体阳性率为94.40%。8个年龄组中,＜1岁组麻疹IgG抗体阳性率最低,为82.24%,其余阳性率均高于93.29%。10个县中以威宁县的麻疹IgG抗体阳性率最低,为79.92%,其余县阳性率均高于91.25%。未接种麻疹类疫苗和接种史不详的人群中麻疹IgG抗体阳性率最低在＜1岁组,接种1剂次以上疫苗后,麻疹IgG阳性率高于95%,接种2剂次与3剂次麻疹类疫苗后阳性率差异无统计学意义（χ2＝0.121,P＝0.728）。结论　加强健康人群麻疹IgG抗体的监测,发现免疫薄弱地区和乡镇是消除麻疹的关键。     

Dynamic of anti-spike receptor binding domain (RBD) levels and short-term adverse events following a heterologous booster dose of BNT162b2 after two doses of CoronaVac in Thai health care workers

BackgroundCoronaVac was administered as the primary COVID-19 vaccine for Thai health care workers (HCWs) in early 2021 in response to the epidemic of new variants. This study aimed to evaluate the dynamic of humoral immune response as well as the short-term side effects resulting from the booster dose of BNT162b2 following completion of a CoronaVac double-dose in Thai HCWs.MethodsThis study was conducted at a teaching hospital in Northern Thailand during August and September 2021. The participants were 50 HCWs who were vaccinated with 2 doses of CoronaVac and were scheduled to receive a booster dose of BNT162b2. Anti-SARS-CoV-2 IgG antibodies levels and short-term side effects were assessed. The anti-RBD level was determined using Architect SARS-CoV-2 IgG II Quant (Abbott).ResultOf the 50 participants, 37 were female. The median age was 33.0 years old. The average time between the second CoronaVac shot and the BNT162b2 booster shot was 81.7 days (SD = 25.0). The median anti-SARS-CoV-2 IgG antibody level on booster vaccination date, as well as day 14, and day 28 after the booster were 335.5 AU/ml, 31,613.5 AU/ml, and 20,311.9 AU/ml, respectively. Fourteen days after the booster, 94% of participants had anti-SARS-CoV-2 IgG antibody levels higher than 50.0 AU/ml. Being female, higher log anti-SARS-CoV-2 IgG antibodies prior to booster vaccination, and longer interval between the second shot and the booster shot were found to be significantly associated with higher levels of anti-SARS-CoV-2 IgG antibodies at both day 14 and day 28 after the booster. There were no reports of serious adverse events.ConclusionA booster dose of BNT162B2 promoted a high level of anti-SARS-CoV-2 IgG antibodies among HCWs who received 2 doses of CoronaVac. The time between the second CoronaVac shot and the booster shot should be at least three months. There were no severe adverse effects observed.     

孕妇血型抗体(IgG)效价检测的临床应用

目的:通过微柱凝胶免疫法,检测不同时期孕妇血清中血型抗体IgG效价水平,辅助临床医生诊断新生儿溶血病,为临床对预防新生儿溶血病的发生而采取的干预治疗措施提供实验室依据。方法:采用微柱凝胶抗人球蛋白试验分别测定妊娠16周、28~32周、36~38周的孕妇血清中IgG血型抗体效价。结果:检测432例“O”型血孕妇血清血型抗体IgG抗A(B)效价>1∶64的例数随着妊娠时间的延长,由142例增加到255例(增加26.2%)。结论:通过微柱凝胶免疫检测法检测孕妇血清血型抗体IgG效价有助于诊断新生儿溶血病,并可据此采取临床干预措施,有利于降低新生儿溶血病的发生率。     

2010年河南省健康人群A群流脑抗体水平监测分析

目的了解2010年河南省健康人群A群流脑抗体水平。方法采用酶联免疫吸附试验(ELISA)检测健康人群血清中的A群流脑抗体含量。结果 A群流脑抗体阳性率为89.1%,抗体平均浓度为6.82μg/mL,其中以5⁶岁组(5.52μg/mL)最低,136岁组(5.52μg/mL)最低,13¹5岁组(8.23μg/mL)最高。结论河南省健康人群A群流脑抗体阳性率维持在较高的水平。