HPV16 E6/E7 Negatively Affect Radiosensitivity of Lung Cancer Cells

Objective Lung cancer cells associated with radioresistance are likely to give rise to local recurrence and distant metastatic relapse,but little is known about its underlying mechanisms.In the present paper,the effects of the HPV16 E6 and HPV16 E7 oncoprotein on the radiosensitivity of lung cancer cell lines were investigated.Methods The HPV16 E6 or HPV16 E7 oncoprotein was expressed by a transient transfection with pcDNA3-HPV16 E6 or pcDNA3-HPV16 E7 expression vector.Human lung cancer H2179 cells and mouse lung cancer Lewis cells were exposed to a γ-ray radiation source,cellular survival was evaluated by using a colony formation assay.The expression of HPV16 oncoproteins E6/E7,extracellular signal-regulated kinases 1/2(ERK1/2) and AKT signaling was determined by Western blot assay.VEGF secretion was determined by ELISA.Results Both HPV16 oncoproteins E6 and E7 significantly decreased radiosensitivity of H2179 cells,associated with a promotion of the ERK1/2 and AKT phosphorylation.A decrease of reactive oxygen species(ROS) and an increase of VEGF levels were observed in the cells expressing the HPV16 oncoproteins E6 and E7.Furthermore,a similar reduction of radiosensitivity mediated by the HPV16 oncoproteins E6 and E7 was also observed in a mouse lung cancer Lewis cells.Conclusion The findings indicate that the HPV16 oncoproteins E6 and E7 negatively affects susceptibility of lung cancer cells to radiotherapy via regulation of the ERK1/2 and Akt signaling pathway and VEGF expression.     

Profiling EGFR in Triple Negative Breast Tumor Using Affibody PET Imaging

Objective Triple negative breast cancer(TNBC) represents a group of refractory breast cancers with aggressive clinical manifestations as well as poor prognoses.Human epidermal growth factor receptor(EGFR) expression is strongly associated with TNBC progression and it may serve as a therapeutic target for TNBC.We aimed to evaluate EGFR affibody-based PET imaging to profile EGFR expression in small animal models.Methods 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid(DOTA) conjugated Ac-Cys-ZEGFR:1907 was chemically synthesized using solid phase peptide synthesizer and then radiolabeled with 64Cu.The in vitro cell uptake study was performed using SUM159 and MCF7 cells.The biodistribution and small animal PET imaging using 64Cu-DOTA-ZEGFR:1907 were further carried out with nude mice bearing subcutaneous MDA-MB-231 and SUM159 tumors.Results DOTA-Ac-Cys-ZEGFR:1907 was successfully synthesized and radiolabeled with 64Cu.Biodistribution study showed that tumor uptake value of 64Cu-DOTA-Ac-Cys-ZEGFR:1907 remained at(4.07±0.93)％ID/g at 24 h in nude mice(n=4) bearing SUM159 xenografts.Furthermore,small animal PET imaging study clearly showed that 64Cu-DOTA-Ac-Cys-ZEGFR:1907 specifically delineated the EGFR positive TNBC tumors at 4 h or later.Conclusion The study demonstrates that 64Cu-DOTA-Ac-Cys-ZEGFR:1907 is a promising molecular probe for PET imaging of EGFR positive TNBC.EGFR based small protein scaffold holds great promise as a novel platform that can be used for EGFR profiling of TNBC.     

Effect of Amifostine on Patients with Lung Cancer Treated with Radiotherapy or Concomitant Chemoradiotherapy: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

Objective Amifostine is clinically used as a chemical radioprotector. Nevertheless, its efficacy as a radioprotector remains controversial. Methods PubMed, Cochrane Central Register of Controlled Trials, EMBASE, China National Knowledge Infrastructure, and the references of the published results of trials on the efficacy of amifostine in patients with lung cancer and who received radiotherapy or concomitant chemoradiotherapy were searched. The pooled radiation protection efficacy, treatment response, and side effects of amifostine were calculated using RevMan software. Results Twelve randomized controlled trials involving 1000 patients with lung cancer were ultimately analyzed. Results of meta-analysis revealed that the use of amifostine reduced the risk of acute esophageal toxicity(RR, 0.56; 95% CI, 0.39-0.81; P=0.002) and pulmonary toxicity(RR, 0.42;95% CI, 0.25-0.70;P=0.001). Subgroup analysis also demonstrated that the risk of acute esophageal toxicity and pulmonary toxicity significantly reduced in patients who received chemoradiation concurrent with amifostine or radiation only. Pooled data showed that the use of amifostine did not significantly decrease the risk of late pulmonary toxicity(RR, 0.74; 95% CI, 0.45-1.19; P=0.210). Moreover, subgroup analysis demonstrated that the risk oflate pulmonary toxicity did not significantly decrease in patients who received chemoradiotherapy concomitant with amifostine(RR, 0.84; 95% CI, 0.48-1.46; P=0.540). Amifostine did not exert tumor-protective effects in partial response(RR,0.98;95% CI, 0.83-1.15; P=0.800) but improved complete response(RR, 1.50; 95% CI, 1.03-2.18; P=0.030), although publication bias was observed through Egger' s test(P=0.000). Moreover, amifostine had no effect on one-year overall survival (RR, 0.94; 95% CI, 0.81-1.09; P=0.400) and two-year overall survival(RR, 1.06; 95% CI, 0.81-1.39;P=0.680) rates. The incidence of neutropenia, a hematologic side effect of amifostine, was not significantly different(RR, 1.02; 95% CI, 0.61-1.71; P=0.940) between the amifostine and control group. The use of amifostine, however, significantly decreased the incidence of thrombocytopenia(RR, 0.45;95% CI, 0.21-0.94;P=0.030). The most common amifostine-related side effects were nausea, vomiting, and hypotension with average incidence rates of 11%, 14%, and 24%, respectively. Conclusions This systematic review showed that the concurrent administration of amifostine with radiotherapy to patients with lung cancer significantly reduced the risks of acute esophageal toxicity and acute pulmonary toxicity and decreased the incidence of thrombocytopenia without tumor-protecting effects. In addition, the toxicities of amifostine were generally controllable through clinical treatment or resting.     

Learning From Biomarkers in Victims Accidentally Exposed to Ionizing Radiation

Biomarkers,such as chromosome aberration and micronuclei assays,prove to be reliable for facilitating clinical diagnosis in radiation accidents.In a radiation accident in India,chromosomal aberration,γ-H2AX,as well as other blood markers,were detected in accidentally exposed victims.This multi-parametric approach aided in confirming that individuals had been exposed by ionizing radiation.However,doses were impossible to estimate because of a 30-day delay in accident awareness.Exposure dose for victims was estimated using a dose-response curve previously established.Dose estimation,blood cell depletion kinetics,and no appearance of prodromal symptoms suggested that doses of exposure were low.Hematologic investigation,sampling time,and chromosome aberration scoring were all proposed according to data from the victims exposed to 60Co.Finally,knowledge regarding chromosome aberration analysis and the importance of international co-operation and assistance should be shared from this accident.     

军用食品研究进展

目的 分析和综述国外军用食品研发动态,为研制我军航空航天食品提供参考和思路.资料来源与选择国外该领域的相关研究论文、文章、综述以及美国政府AD报告. 资料引用引用论文、文章和综述以及美国政府AD报告共23篇. 资料综合近年来外军军用食品研究进展很快.通过采用多项高新技术,例如超高压杀菌技术、微波技术、微胶囊包埋技术、食品自加热技术和食品包装新材料.包括高阻隔包装材料与吸收氧气材料有机结合以及食品储存技术的综合应用不断推进军用食品革新.其主要目标是提高食品长时间食用的感官接受性,并改进包装以有效延长食品保质期,使食品更加适合在各种战场环境中食用.此外,研发通过皮肤吸收的营养输送系统等新概念食品为研发军用食品提供了崭新的思路和理念,尽管距离实用阶段还有一段距离.外军军用食品研究新思路体现在给食品赋予抗疲劳和增强免疫力等多种功能性;完善餐谱多样化以满足部队战场多样化需求. 结论 外军将高新技术应用于军用食品研发领域,使军用食品不断改进,主要集中在提高食品接受性、延长贮存期、研发新概念食品、开发自热食品和研制营养模块,并且注重系列化、餐谱化、功能化.这些最新研发动向对我军研制航空航天食品具有借鉴和参考价值. Abstract： Objective To analyze and overview the latest research and development of foreign military rations so as to provide ideas and references to our aviation and space military ration.Literature iesources and selection Foreign theses,research articles,reviews and U.S.government AD reports in the food related fields.Literature quotation Twenty-three pieces of thesis.paper article and review were cited.Literature synthesis The researches of military ration have been rapidly developed in recent years.Many high-technologies,such as ultra-high pressure sterilization technology,microwave technology,microcapsule embedding technology,self-heating ration technology and new food packing materials,including high-separating packing materials,oxygenabsorbing materials and food storage technology,which brought a great deal of innovations for military food have been adopted.The main aims of these researches are to increase the food acceptance for long time consumption.upgrade packing to prolong the shelf life in order to make rations more suitable to eat in different battle-field environments.Moreover,although it still needs time to step in practical application,but researching on new concept ration,such as delivery system of the nutrients under subcutaneous,is providing brand-new idea for military ration research and development.The new research of military ration also focused on food functions,such as anti-fatigue and immune enhancing function,and the development of more food menus to guarantee the military requirement in different battle fields. Conclusions High technologies have been used in military ration development to constantly improve its applicability.The main research issues have been focused on high acceptability,longer shelf life,new concept ration,self-heating ration and nutriment pack.Special attention has been paid to develop more menus to the ration and more special purpose-ration as well as add the components with physiological function to ration.All these information can be used as reference for our development of aviation and space military ration.     

竹笋味美又疗疾

竹笋,即竹的嫩芽茎,古人称之为"竹肉"、"竹胎"、"竹芽"。竹笋可分为冬笋、春笋、鞭笋3类:冬季藏在土中的为冬笋,色泽洁白,肉质细嫩,鲜美无比,有"笋中皇后"之誉;春天     

武警浙江总队医院30年帮困奏响和谐强音

本刊讯 武警浙江总队医院在认真做好部队官兵医疗保障的同时,连续30年开展为驻地困难家庭送温暖、送健康、献爱心活动,赢得了群众高度赞誉.今年春节前夕,该院在去年帮扶310户困难家庭的基础上,进一步加大了帮扶力度,出资22万余元,对嘉兴市区410户困难家庭开展献爱心行动.     

《创伤外科杂志》出版杂志邮寄快速到付的通知

因邮局平邮方式常常发生邮局误丢、误邮事件，故从2015年7月开始，在《创伤外科杂志》发表文章的作者均赠送2本杂志（内附版面费发票），邮寄方式一律采用快递到付的形式（邮资由作者支付）。来稿请附作者姓名、单位省市名称和邮编、详细通讯地址、电话、email。     

阳性MR淋巴造影检测隐匿转移性淋巴结的实验研究

目的 初步探索皮下应用Gd-DTPA-白蛋白的阳性磁共振淋巴造影检测隐匿转移性淋巴结的价值.方法 建立腘窝隐匿转移性淋巴结新西兰兔模型,分析隐匿转移性淋巴结在平扫及皮下注射0.10 mmol/肢Gd-DTPA-白蛋白后24 h时的MRI特征,并与病理检查对照.结果 12只兔的腘窝隐匿转移性淋巴结模型成功建立,其淋巴结大小为(6.7±0.2)mm.平扫时隐匿转移性腘窝淋巴结表现为T1WI上等信号,T2WI上高信号,与正常淋巴结相似.Gd-DTPA-白蛋白增强24 h,正常淋巴结呈明显、均匀的强化,而隐匿转移性淋巴结呈不均匀强化,强化形式有环状(4枚淋巴结)、云絮状(3枚)和不规则偏心"充盈缺损"(3枚),另有2枚淋巴结不强化.在脂肪抑制T1WI像上淋巴管呈明亮高信号,转移性淋巴结的引流淋巴管明显迂曲扩张.结论 皮下应用Gd-DTPA-白蛋白的阳性MR淋巴造影可清晰显示淋巴结和淋巴管,可用于检测隐匿转移性淋巴结.     

补充维生素B2对急性低氧暴露小鼠血浆代谢组的影响

目的 观察补充维生素B2对急性低氧暴露小鼠外周血代谢组的影响.方法 采用随机数字表法将35只雄性昆明小鼠随机分为:正常对照组,低氧对照组,2倍、4倍及8倍维生素B2补充组,每组7只.以相应饲料(维生素B2含量分别为6 mg/kg、12 mg/kg、24 mg/kg和48 mg/kg)喂养2周后,除正常对照组外,其它各组均在模拟6000 m高度停留8 h,采集血浆,以核磁共振的方法分析其代谢组变化.结果 急性低氧暴露后,各组动物血浆代谢组在得分图中呈聚类型分布,且有先分离后同归的代谢模式变化轨迹,显示出补充不同剂量维生素B2后小鼠血浆代谢组逐渐恢复的趋势.代谢模式产生差别的原因是脂类、乳酸、丙氨酸、N-乙酰糖蛋白、谷氨酸、胆碱、牛磺酸、糖、肉碱、甘氨酸和肌酐等物质的水平发生了变化,表明相关的代谢途径发生了变化.结论 补充维生素B2使急性低氧暴露机体碳水化合物、脂肪、蛋白质代谢发生改变,而维生素B2可以改善碳水化合物代谢,并可能通过肉碱间接调节了脂肪代谢;同时还发现一些氨基酸代谢发生显著变化. Abstract： Objective To observe the effects of vitamin B2 supplementation on plasma metabonome of the mice which exposed to acute hypoxia. Methods Thirty-five male Kunming mice were randomly and averagely divided into 5 groups, among which the control and hypoxia control groups were fed the fodder containing 6, 12, 24, 48 mg/kg vitamin B2 respectively. All groups were exposed to simulated hypoxia environment (equivalent to 6000 meters above sea level) for 8 hours except control group. Nuclear magnetic resonance spectrometer was used to test the change of metabonome from collected plasma. Results After acute hypoxia exposure, the metabonome pattern in all groups showed clustering distribution in scores plot and the metabonome changes were along the trail from segregation to regression. These indicated a gradual recover tendency on plasma metabonome when different doses of vitamin B2 supplemented. The changes of lipids, lactic acid,alanine, N-acetyl-glycoprotein (NAC), glutamate, choline, taurine, glucose, carnitine, glycine and creatinine were among the factors contributing significantly to the difference of metabonome pattern and indicated the changes of related metabolic pathway. Conclusions The metabonomes of carbohydrate, lipid and protein of acute hypoxia mice are improved by vitamin B2 supplementation.Vitamin B2 is helpful to improve carbohydrate metabolism and indirectly accommodate lipid metabolism through carnitine. Vitamin B2 supplementation also results in the significant change of amino acid metabonome.     

外伤后膀胱、前列腺、尿道内多发巨大结石1例

1病历简介 患者，男，43岁。因间断性排尿困难半月余，伴尿频、尿急、尿痛入院。患者于12年前因骨盆骨折合并尿道断裂在我院行尿道修补术，术后排尿功能良好。术后第3年出现排尿困难，经2次尿道扩张术后，排尿正常。查体：体温37．5℃，心、肺、腹无异常。外科情况：双脊肋角不饱满，双肾区轻微扣击痛，沿输尿管走行无压痛及扣击痛，下腹部正中可见8．0cm长的纵向愈合伤口。     

艾滋病病毒感染合并肺结核1例

患者 男,28岁,菲律宾人,船员.以发热、干咳、胸闷1周入院.摄胸部正、侧位片提示间质性肺炎,检测血清抗HIV( ),痰结核杆菌( ). 胸片显示:胸廓饱满、对称,双肺透光度减低,可见对称性网格状模糊阴影,双肺野弥漫性均匀分布类椭圆形结节状高密度影,心膈尚可(图1,2).     

西米替丁致固定性红斑1例

1 病例介绍 患者,男性65岁.因突发上中腹持续性剧烈疼痛6h,持续不缓解于2006年2月10日收住.无发热、皮肤黄染、出皮疹.住院前未服药,入院查血淀粉酶诊断急性胰腺炎.给予静点西米替丁和氨苄青霉素治疗.用药2d后左大腿前面皮肤出现一直径3cm环形丘疹性充血斑,中心皮肤苍白.     

高原驻训"十防"

高原地区具有海拔高、严寒、缺氧、低气压、低温、低湿、强气流、紫外线强、地形复杂等特点,部队在进驻高原地区实施训练时,需要周密计划、精心安排、严密组织、合理施训。高原驻训要"十防":防紧张在部队离开营区进驻高原实施训练的前2周,需要对所有参训人员进     

飞行人员血尿酸与心血管危险因素的关系

目的 探讨飞行人员血尿酸与心血管危险因素的关系.方法 对象为606例男性飞行人员,测量身高、体重、血压、胸围、腰围及臀围;实验室检测血尿酸、血脂、血糖、尿素、肌酐及生化参数,并进行包括生活习惯、个人及家族疾病史、飞行负荷的问卷调查.将血尿酸水平分为4层:≤298、299～357、358～417、＞417 μmol/L,最后1层为临床诊断高尿酸血症标准.以血尿酸为自变量,其他多种心血管危险因素为因变量,分析血尿酸与这些危险因素的关系.结果 飞行人员高尿酸血症患病率16.67%.血尿酸与临床化学标志物TG、TC、载脂蛋白A1、载脂蛋白B、空腹血糖、肌酐、总蛋白、γ-转肽酶、碱性磷酸酶、总胆汁酸、乳酸脱氢酶和丙氨酸转氨酶呈正相关(r=0.65、0.24、0.22、0.42、0.29、0.11、0.33、0.25、0.17、0.17、0.11、0.10),临床化学标志物均值随血尿酸水平升高而增高;与年龄、BMI、胸围、腰围、臀围及收缩压呈正相关(r=0.11、0.24、0.19、0.19、0.17、0.14),生理参数均值亦随血尿酸水平升高而增高.吸烟量≥20支/d者高PUA血症患病率高于吸烟量＜20支/d者(x2=13.14,P＜0.01).有心脑血管病家族史者高PUA血症患病率高于其他人员(x2=9.506,P＜0.01).多元回归和Logistic回归分析显示血尿酸水平与TG和肌酐水平独立相关.结论 高尿酸和高TG血症是动脉粥样硬化形成和发展的独立危险因素;与血尿酸相关的载脂蛋白B,空腹血糖升高,收缩压升高,体质指数、胸围、腰围和臀围超标,吸烟量大和相关家族史构成心血管疾病的综合危险因素. Abstract： Objective To investigate the relationship between plasma uric acid (PUA) level and cardiovascular risk factors for Chinese military flying personnel. Methods Six hundred and six flying personnel served as subjects and their blood pressure, height, weight, chest circumference (CC), waist circumference (WC), hip circumference (HC) were measured. PUA, blood fat, blood glucose, carbonylamines, creatinine and other biochemical indicators were tested. The information,such as living habit, personal and family disease history, as well as workload in flight was got by questionnaire. PUA level was divided into four classes: ≤298, 299-357, 358-417 and ＞417μmol/L. The last one was taken as the clinical diagnostic standard for hyperuricemia. The relationship between PUA level and chosen risk factors was analyzed by one-way ANOVA, Chi-square or t-test and multiple regression or binary logistic regression. Results The incidence of hyperuricemia was 16. 67% in Chinese military flying personnel. PUA levels were positively correlated with clinical biochemical parameters: TG, TC, apolipoprotein A1 (ApoA1), apolipoprotein 13 (ApoB), fasting blood glucose (FBG), creatinine, total protein, gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP), total bile acid (TBA), lactate dehydrogenase (LDH) and ALT(r=0.65, 0.24,0.22, 0.42, 0.29, 0.11, 0,33, 0.25, 0.17, 0.17, 0.11, 0.10 respectively). The mean values of these clinical biochemical flag parameters, corresponding to different PUA classes, were gradually increased with PUA level raise. PUA level was positively correlated with age, body mass index (BMI), CC, WC, HC, and systolic blood pressure (SBP) (r=0. 11, 0.24, 0. 19, 0. 19, 0. 17, 0. 14 respectively). The incidence of hyperuricemia is higher in the group of smoking cigarette ≥ 20 bars/day (x2 = 13.14, P＜0.01 ) and in the group with family cardiovascular disease history (x2 =9. 506, P＜0. 01). Multiple regression and binary Logistic regression analysis revealed that the relationship of PUA concentration with triglyceride and creatinine was independently correlated. Conclusions Hyperuricemia and hypertriglyceridemia were the independent risk factors of causing and driving arteriosclerosis. Other PUA related ingredients, such as high ApoB, high FBG, high SBG, super CC,WC and HC, over weight, smoking habit and family disease history are composed of multiple risk factors of resulting in cardiovascular diseases.     

超选择性肾动脉栓塞治疗肾活检后大出血1例

报告1例应用超选择性肾动脉栓塞抢救肾活检后大出血成功,提示在肾活检率逐年提高、肾活检人数逐渐增多的今天,即使发生严重的大出血,不再必须切除肾脏,介入治疗可以取得良好效果,而且能够尽可能地保护残存的肾功能.     

多中心网状组织细胞增生症一例

病例资料患者,女,71岁,1年前颜面部无明显诱因出现红斑,瘙痒,无渗液,搔抓后头、颈、躯干逐渐出现红斑、丘疹、结节。查体:四肢、腹部水肿。双手指部分变形。头皮、颜面、躯干、背部紫红色斑、丘疹密集,呈米粒大小。实验室检查:血沉65mm/h。ASO(-)、RF(-)、CRP59mg/l。ANA、抗ds     

巨大膀胱憩室内结石合并憩室颈部膀胱癌1例

患者男性,54岁,下腹部不适两年,尿频、尿急等症状,时有下腹疼痛,近日疼痛逐渐加重,尿液检查:少量红细胞.     

食管炎性假瘤1例

患者男性,23岁.间断恶心、呕吐伴进食后哽噎感、嗳气半年,给予中药治疗,症状无明显缓解.查体:腹软,中上腹轻微压痛,无反跳痛、腹肌紧张,余无异常.胃镜:距门齿40～45cm处食管环周2～7点可见半球样隆起,累及齿线,隆起表面部分粘膜尚光滑,可见血管纹理,隆起下部表面粘膜粗糙不平,呈增殖样改变,组织较韧,易出血.