全蝎抗凝活性成分的定性分析

目的鉴定与探讨全蝎抗凝活性成分。方法用不同的展开剂，采用薄层色谱法和纸层析法对全蝎抗凝活性成分进行鉴定和分析。结果全蝎抗凝活性成分中不含生物碱、糖类、甾体和萜类，含蛋白质和多肽，但在氨基酸的定性检测中，分离得到6个成分的斑点，与14种氨基酸对照品在相同位置上有相同颜色的斑点。结论全蝎抗凝活性成分中含有多种氨基酸、蛋白质和多肽。     

地埝药材薄层色谱鉴别研究

目的：研究中药地埝的薄层色谱鉴别方法.方法：依据现行《中国药典》2010年版一部附录ⅥB试验方法考察了不同提取方法、薄层板、展开剂、显色剂下地埝的薄层鉴别研究.结果：用硅胶GF254薄层板,二氯甲烷-乙酸乙酯-甲酸（5：4：1）为展开剂,以氨熏为显色剂,再置可见光下检视斑点,分离效果最佳.结论：本实验研究可作为地埝药材质量标准的提高与完善提供依据.     

紫杉中紫杉烷和双黄酮类成分的薄层色谱鉴别

目的：建立紫杉药材中紫杉烷类和双黄酮类成分的薄层色谱鉴别法。方法：以紫杉宁为对照品,石油醚-乙酸乙酯-丙酮-甲醇（8∶2∶2∶1）为展开剂系统,用硅胶G板鉴别紫杉烷类成分;以金松双黄酮和银杏双黄酮为对照品,石油醚-乙酸乙酯-甲醇（4∶1∶1）为展开剂系统,用聚酰胺薄膜鉴别双黄酮类成分。结果：本方法斑点清晰,重复性好,并可将紫杉区别于一些松、杉、柏类植物的枝叶。结论：本方法简便,快速,可用于紫杉药材的质量控制。     

露兜簕的质量标准研究

目的：建立露兜簕的质量标准。方法：通过对露兜簕的性状、显微鉴别、薄层色谱鉴别的研究建立露兜簕的质量标准。结果：按照薄层色谱法用硅胶G薄层板为固定相,以环己烷-乙酸乙酯（6∶1）为展开剂,喷以1%三氯化铝乙醇溶液,在105℃加热5min,置紫外光灯（365nm）下检视。供试品色谱中,在与对照药材色谱图相应的位置上显相同颜色的荧光斑点。结论：本文建立的方法能够有效控制露兜簕的质量。     

龙葵药材薄层鉴别研究

目的研究中药龙葵的薄层鉴别方法。方法依据现行《中国药典》2010年版一部附录ⅥB试验方法考察了不同提取方法、薄层板、展开剂、显色剂下龙葵的薄层鉴别方法。结果用硅胶G薄层板．二氯甲烷一甲醇一氨水（8：1．5：0．4）为展开剂,以10％硫酸乙醇试液显色,再置紫外灯（365nm）下检视斑点,分离效果最佳。结论本实验研究可作为龙葵药材质量标准的修订内容。     

薄层层析测定胆汁中胆固醇

本文介绍一种薄层分离-斑点洗脱-比色测定胆汁中胆固醇的方法。玻板铺硅胶G;展开剂异辛烷:醋酸乙酯为2:1(V/V)。胆固醇Rf值0.52,展开后取下胆固醇斑点,比色测定。本法样品用量少,准确度高。     

猪牙皂的薄层色谱鉴别研究

目的:确定猪牙皂薄层色谱鉴别的实验条件.方法:采用3种不同的展开剂分别进行猪牙皂薄层色谱鉴别研究.结果:猪牙皂薄层色谱条件为:采用硅胶G板,以甲苯-醋酸乙酯-甲酸(14∶4∶0.5)为展开剂展开,10%硫酸乙醇溶液为显色剂,在105℃加热至斑点显色清晰.结论:本实验精密度好且操作简便易行.     

薄层色谱法鉴别去痛片四种主要成分

目的 用薄层色谱法鉴别去痛片中的四种主要成分。方法 将相同浓度的供试品溶液与对照品溶液点于同一硅胶GF254薄层板上，采用薄层色谱法，观察各主斑点的位置、大小及颜色。结果 去痛片中的四种主要成分彼此分离良好，且分别与各自的对照品溶液的主斑点耐值相同，大小、颜色相同。结论 用薄层色谱法可同时鉴别去痛片中的四种主要成分且方法简单、省时、实用。     

桃红四物汤醇提液的薄层分离与定性鉴别

目的：桃红四物汤醇提液的薄层分离与定性鉴别。方法：采用薄层色谱法,选用硅胶G 薄层板,以正丁醇- 冰乙酸- 水（4∶1.5∶5,上层）为展开剂,置紫外灯（365 nm）下检视,观察桃红四物汤醇提液中化学成分的展开情况,再选用碘蒸气对各斑点进行显色,并将薄层板置于薄层扫描仪进行扫描,记录各斑点的吸收光谱图与比移值,与对照品进行观察对比。结果：桃红四物汤醇提液共分离得到5 个斑点,其中定性鉴别出3 个斑点,分别为来自红花的羟基红花黄色素A、来自白芍的芍药苷、来自当归和川芎的阿魏酸;其它2 个斑点在组成桃红四物汤的六个单味药中均未检出。结论：薄层色谱法能实现对桃红四物汤醇提液中化学成分的分离,可在此基础上进一步进行新成分的确定,分离方法简单快速、稳定可行。     

复方丹参片和复方丹参颗粒的三七薄层的研究

目的:对复方丹参片和复方丹参颗粒的三七薄层的研究情况进行分析探讨。方法:研究中分别采取不同的提取方法、展开剂以及展开条件对相同的复方丹参片和复方丹参颗粒展开薄层色谱实验,并挑选出最佳实验条件。结果:最终确定采用硅胶G高效预制薄层板进行分离,展开剂为:以二氯甲烷-无水乙醇-水(70:45:6.5),相对湿度18%的条件下为最佳实验条件,人参皂苷Rg1,人参皂苷Rb1,人参皂苷Re以及三七皂苷R1均存在清晰斑点,分离效果良好。结论:采取以上薄层色谱法展开实验,具有良好的专属性,可以对该类制剂的质量进行严格的控制,值得关注。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.