BMP-2,3,4,5在颌面部神经鞘瘤中的表达分析

目的：探讨ＢＭＰｓ家族成员与外周神经肿瘤的关系。方法：用原位杂交方法对ＢＭＰ－２，３，４，５在人良、恶性神经鞘瘤中的表达进行观察。结果：显示ＢＭＰ－２，３，４，５ｍＲＮＡ在外周神经的良恶性神经鞘瘤中均有分布。良性肿瘤中，呈栅栏状排列，肿瘤细胞聚集的区域ＢＭＰｓ表达升高；恶性肿瘤中ＢＭＰｓ的表达高于良性肿瘤。结论：确定了ＢＭＰｓ在外周神经肿瘤中的表达和分布，揭示部分ＢＭＰｓ可能参与了外周神经肿瘤的发     

多形性腺瘤中BMP的表达及作用机制分析

目的：检测多形性腺瘤中ＢＭＰ蛋白的表达及ＭＢＰ２ ｍＲＮＡ的扩增情况,以探讨多形性腺瘤的病理发生机制。方法：应用ＢＭＰ单克隆抗体检测ＢＭＰ在多形性腺瘤中的表达,并进一步应用ＭＢＰ２的特异性引物和ＲＴ－ＰＣＲ的方法,扩增６例多形性腺瘤组织中ＲＮＡ。结果：６例多形性腺瘤组织中可以检测到ＢＭＰ蛋白的扩增,其中５例有ＢＭＰ２ ｍＲＮＡ扩增。结论：ＢＭＰ可能参与了多形性腺瘤的病理机制,ＢＭＰ的存在可能是上皮     

骨形成蛋白及其mRNA在成釉细胞瘤中的表达

目的：检测成釉细胞瘤中的ＢＭＰ的表达,探讨成釉细胞瘤的发生及病理机理。方法：采用免疫组化和原位杂交的方法,检测２４例成釉细胞瘤标本中ＢＭＰ蛋白和ＢＭＰ－２、－４ｍＲＮＡ表达。结果：成釉细胞瘤中的柱状上皮层和星网状细胞层中均有ＢＭＰ－２、－４ｍＲＮＡ和ＢＭＰ蛋白的表达,ＢＭＰ－２、－４ｍＲＮＡ的杂交信号强度在柱状上皮层小于星网状细胞。结论：ＢＭＰ的表达为探讨成釉细胞瘤的来源提供了分子基础;ＢＭＰ可能参与了肿瘤病理机理及生物学行为的形成。     

地鼠颊粘膜癌变过程中增殖细胞核抗原及核仁组成区表达变化实验研究

目的：探讨癌变过程中增殖细胞核抗原（ＰＣＮＡ）、核仁组成区（ＮＯＲ）的表达变化。方法：利用地鼠颊粘膜癌模型采用免疫组化ＬＳＡＢ法检测ＰＣＮＡ,阳性对照为人类炎性增生淋巴结标本,阴性对照为ＰＢＳ代替单抗。采用硝酸银染色法检测ＮＯＲ。结果：①正常粘膜ＡｇＮＯＲ颗粒主要见于基底层细胞,以单一型为主。重度异常增生以聚集型为主。浸润癌以混合型为主。②随恶变进展,ＰＣＮＡ表达逐渐增强,重度异常增生阳性细胞见于上皮全层。结论：ＰＣＮＡ与ＡｇＮＯＲ表达有相关性（ｒ＝０．６３５,Ｐ〈０．００１）,但ＰＣＮＡ更为直观、清晰,实用价值更大。     

生物可吸收性聚合物及其在口腔医学中的应用

生物可吸收性聚合物及其在口腔医学中的应用ＡＰＰＬＩＣＡＴＩＯＮＯＦＢＩＯＬＯＧＩＣＡＬＲＥＳＯＲＢＡＢＬＥＰＯＬＹＭＥＲＩＮＯＲＡＬＭＥＤＩＣＩＮＥ李笑梅综述彭玉田周中华审校１概述生物可吸收性聚合物是指一类生物相容性好，在体内化学或酶作用下可降解的聚...     

小型金属板在下颌骨骨折延迟手术中应用——附23例临床分析

小型金属板在下颌骨骨折延迟手术中应用——附２３例临床分析ＣＬＩＮＩＣＡＰＰＬＩＣＡＴＩＯＮＯＦＭＩＮＯＲＭＥＴＡＬＰＬＡＴＥＩＮＤＥＬＡＹＥＤＯＰＥＲＡＴＩＯＮＯＦＭＡＮＤＩＢＵＬＡＲＦＲＡＣＴＵＲＥ作者单位：兰州医学院第二附属医院口腔科（７３００３...     

人牙髓血管内皮细胞骨形成蛋白—3基因表达的研究

目的：研究ＢＭＰ３ｍＲＮＡ在人牙髓血管内皮细胞中的表达。方法：利用地高辛甙元标记的ＢＭＰ３ｃＤＮＡ探针进行原位分子杂交。结果：正常牙髓血管内皮细胞有ＢＭＰ３ｍＲＮＡ阳性杂交信号出现。该信号在龋坏或炎症状态下增强，并在血管周围的未分化间充质细胞中同时出现，但血管瘤组织内血管内皮细胞未发现该阳性信号。结论：人牙髓的血管内皮细胞能合成分泌ＢＭＰ３，可能参与了牙髓的自身稳定和修复过程；ＢＭＰ３ｍＲＮＡ在机体内的分布与组织细胞的胚胎起源和分化状态相关。     

牛血浆FN对人牙髓成纤维细胞骨形成蛋白和碱性磷酸酶分泌和表达的影响

目的：观察纤维粘连蛋白（ＦＮ）与牙髓细胞分化的关系．方法：将传代的人牙髓细胞接种于９６孔板,分别用不同浓度牛血浆ＦＮ（１０,２０,４０,８０μｇ／ｍｌ）作用３天,进行骨形成蛋白（ＢＭＰ）和碱性磷酸酶（ＡＬＰ）的免疫组化染色,图象分析仪半定量测定,并测定培养上清液中ＡＬＰ活性．结果表明：四种浓度的ＦＮ组的牙髓细胞ＢＭＰ和ＡＬＰ表达无明显差异,ＦＮ（２０,４０μｇ／ｍｌ）组培养上清液中ＡＬＰ活性较强．结论：ＦＮ在牙髓细胞分化过程中的作用是有限的．     

补骨脂素与维甲酸联合应用对Mc3细胞膜表面四种凝集素受体表达的影响

目的 研究８－ＭＯＰ与ＡＴＲＡ联合应用对Ｍｃ３细胞膜表面凝集素受体表达的影响。方法 免疫组织化学染色方法。结果 对照组凝集素受体表达分别为：ＰＮＡ（＋￣＋＋）,ＳＢＡ（＋￣＋＋）,ＲＣＡ（＋￣＋＋）;８－ＭＯＰ组凝集素受体表达分另为：ＰＮＡ（－￣＋＋）,ＵＥＡ（＋）,ＲＣＡ（＋￣＋＋）;ＡＴＲＡ组表达分别为：ＰＮＡ（－￣＋＋）,ＵＥＡ（＋）,ＳＢＡ（－￣＋＋）,ＳＢＡ（＋￣＋＋）,ＲＣＡ（＋）,８     

BMP2 mRNA在颌骨骨肉瘤及软骨肉瘤中的扩增及其对肿瘤增殖？…

目的 研究ＢＭＰ２ ｍＲＮＡ在颌骨骨肉瘤及软骨肉瘤中的扩增及其对肿瘤预后的增殖活性的影响。方法 应用ＢＭＰ２的ｃＤＮＡ探针，原位杂交检测ｍＲＮＡ的扩增。结果 ３２例标本中有２１例表达ＢＭＰ２ ｍＲＮＡ，有ＢＭＰ２ ｍＲＮＡ表达的病例更容易发生淋巴结转移，且有较高增殖活性。结论ＢＭＰ２可能在骨肿瘤的发生和发展过程中起一定作用，可以作为估计骨肉瘤预后的参考指标。     

阿霉素注射治疗老年三叉神经痛的临床观察

目的:探讨阿霉素外周神经分支注射治疗老年原发性三叉神经痛的疗效。方法:对56例老年原发性三叉神经痛患者进行外周神经分支注射阿霉素治疗,1年后观察疗效。结果:随访到54例,其中48例疼痛缓解,疼痛缓解率为88.89%。结论:阿霉素外周神经分支注射治疗老年原发性三叉神经痛创伤小、并发症少,效果可靠。     

三叉神经痛末梢支脱髓鞘的超微结构研究

目的 为了探讨三叉神经痛与末梢支脱髓鞘、感染、缺血及神经管内压力持续增高的关系。方法 通过对１０例三不梢支的脱髓鞘染色,光镜观察,（５例无痛的舌神经,５例疼痛的下牙槽神经）,８例电镜观察,（眶下神经痛４例,下牙槽神经痛４例）,２２例病理性骨腔刮汉物Ｈ,Ｅ染色光镜观察,结合临床症状深入研究三叉神经痛的周围病因。结果 １８例末梢神经都发现有脱髓鞘改变。无痛的舌神经与疼痛的下牙槽神经末梢支脱髓鞘程度无显     

The peripheral distribution of trigeminal nerve fibres in the rat temporomandibular joint studied by an anterograde axonal transport method with wheat germ agglutinin-horseradish peroxidase.

Wheat germ agglutinin-horseradish peroxidase was injected into the trigeminal ganglion to trace the peripheral distribution of the nerve fibres in the temporomandibular joint. It was transported anterogradely along trigeminal nerve fibres. Horseradish-peroxidase-labelled nerve fibres were found in the anterior and posterior bands of the articular disc, and terminated as nerve endings near the intermediate zone of the disc. However, the intermediate zone itself did not contain any nerve endings. Other nerve fibres penetrated from the subsynovial layer into the synovial membrane and also terminated as nerve endings close to the articular cavity. Thus, this method is suitable for tracing peripheral nerve fibres and nerve endings originating in the trigeminal ganglion.     

Peripheral painful traumatic trigeminal neuropathies

The aim of this article is to review the clinical, pathophysiological, and therapeutic aspects of traumatically induced trigeminal nerve pain. We introduce a new and, in our view, more accurate terminology: peripheral painful traumatic trigeminal neuropathy (PPTTN) to define this patient group. The proposed pathophysiology of PPTTN is largely based on studies in spinal nerve injury models. However, trigeminal nerve injury studies have shown some subtle differences in response to physical and inflammatory insults, and these are discussed. The treatment of painful neuropathies is difficult and carries a poor prognosis. Based on the available literature on efficacy and side effects, we propose a treatment algorithm for traumatic trigeminal neuropathies.     

CD16参与大鼠外周神经损伤及痛觉过敏的研究

目的:观察实验性大鼠神经损伤过程中CD16在三叉神经节的动态表达和分布,探讨三叉神经系统通过神经免疫反应参与外周神经损伤及痛觉过敏的潜在机制。方法:建立大鼠眶下神经慢性压榨损伤动物模型,应用免疫荧光标记观察CD16在三叉神经节的表达和分布,通过与CGRP的相关性分析,检测痛觉过敏过程中CD16的参与作用。结果:大鼠眶下神经压榨性损伤72 h后,三叉神经节初级感觉神经元CD16的表达显著性增强,5 d后达峰值,且神经元内CD16的表达与CGRP的表达具有显著相关性。结论:三叉神经系统初级感觉神经元细胞表达CD16,并参与外周神经损伤和痛觉过敏过程。     

Microanatomic analysis of the trigeminal nerve and potential nerve graft donor sites

A histologic study was undertaken to define the microanatomic characteristics of two commonly injured peripheral trigeminal nerve branches (lingual and mandibular nerves) and the two nerves most frequently procured for use in their interpositional graft repair (sural and greater auricular nerves). Nerves, obtained from fresh human cadavers, were evaluated for total fascicular area, fascicle number, axon number, axon size, and axon density. The peripheral branches of the trigeminal nerve (third division) were morphometrically similar, with only a slight decrease in axon density in the lingual nerve. Comparisons between the donor nerves, however, showed numerous discrepancies at the axonal level. While the fascicular area of the sural nerve was only slightly smaller, axon numbers and densities were only one-half that of the trigeminal nerves. Although the greater auricular nerve was appreciably smaller in overall size, a much better correlation existed with the trigeminal nerve in axonal qualities. These microanatomic findings raise questions about the potential capability of these graft choices to optimally restore axonal connections between nerve ends in trigeminal nerve repair.     

三叉神经第Ⅲ支多分支撕脱术治疗三叉神经痛疗效观察

目的:探讨三叉神经第Ⅲ支多分支撕脱术治疗三叉神经痛的疗效。方法:对29例原发性三叉神经第Ⅲ支痛患者,采用口内翼下颌韧带旁升支前缘径路,并附加颏孔区前庭沟径路,实施三叉神经第Ⅲ支多分支撕脱。结果:术后观察0.5～5年未见复发,分支撕脱区域仍有麻木感。结论:对原发性三叉神经第Ⅲ支痛采用三叉神经第Ⅲ支多分支撕脱术,可以提高治疗效果,是一种值得选择的方法。     

Selective anesthesias of peripheral branches of the trigeminal nerve due to odontogenic infection

Infection of dental origin may induce anesthesias of peripheral branches of the trigeminal nerve and should be considered in the differential diagnosis of such neuropathies. This article presents two cases in which focal odontogenic infection caused sensory disruption at peripheral points along the maxillary and mandibular divisions of the trigeminal nerve. In both cases resolution of symptoms followed extraction of teeth responsible for the infection.     

三叉神经周围支改良撕脱术35例初步报告

目的:探讨三叉神经痛周围神经撕脱术的手术方法。方法 :对35例原发性三叉神经痛周围支病变的患者,采用周围神经撕脱术的改良术式:准确定位、广泛撕脱、大面积分离、返流支损毁、钛钉神经孔封堵及术后镇痛不全的甄别处理。结果:术后观察1～4年,复发3例。结论 :改进术式复发率低,疗效好,值得推广。