腺苷对冠心病患者白细胞CD11b/CD18表达的影响

目的探讨不同剂量腺苷对不同类型冠心病患者单核细胞及中性粒细胞表面CD11b/CD18表达的影响。方法选择经临床症状、心电图改变及心肌酶学确诊的冠心病患者及正常健康者采血,患者血液分别给予不同剂量的腺苷进行干预,免疫荧光标记后用流式细胞仪分别检测正常人及患者用药前后单核细胞与中性粒细胞CD11b/CD18的表达。结果冠心病患者中性粒细胞与单核细胞CD11b/CD18的表达较正常人明显升高(P<0.01),而不稳定型心绞痛与急性心肌梗死患者其CD11b/CD18表达差异无统计学意义(P>0.05);腺苷浓度在10、30、100μmol/L能有效降低冠心病患者单核细胞及中性粒细胞CD11b/CD18的表达(P<0.05),但30、100μmol/L组之间差异无统计学意义(P>0.05)。结论冠心病的发生可能与CD11b/CD18表达升高有关,腺苷可通过降低CD11b/CD18的表达而降低白细胞与内皮细胞之间的黏附性。     

白细胞CD11b/CD18在急性冠状动脉综合征患者中的表达

目的　观察外周静脉血中性粒细胞和单核细胞膜CD11b/CD18表达在急性冠状动脉综合征 (ACS)患者中的变化 ,探讨其与斑块稳定性的关系。方法　选 30例ACS患者 (ACS组 )、2 4例稳定型心绞痛患者 (SA组 ) ,30例冠状动脉造影阴性作对照组。流式细胞仪直接免疫荧光法检测CD11b/CD18的表达。结果　中性粒细胞和单核细胞膜CD11b/CD18的表达ACS组显著高于SA组(P <0 0 1)和对照组 (P <0 0 1)。结论　ACS患者中性粒细胞和单核细胞膜CD11b/CD18的表达增加与斑块不稳定性有关     

动脉粥样硬化时低密度脂蛋白对单个核细胞表面粘附分子表达的影响

目的：为探讨动脉粥样硬化中低密度脂蛋白（LDL）对单个核细胞上粘结附分子表达的影响。方法：用碱磷酶－抗碱磷酶桥联酶染色法检测30例冠心病病人外周血单个核细胞表面CD11a、CD11b和CD18,与血LDL水平作相关分析,并观察高浓度LDL血清对单个核细胞表面该3种分子表达的影响。结果：发现动脉粥样硬化时LDL及CD11a、CD11b、CD18均显著高于正常人（P分别＜0．05,＜0．01,＜0．001和＜0．001）;LDL水平与CD11a、CD18显著相关,（P分别＜0．01和＜0．05）;高浓度LDL血清处理后单个核细胞表达CD11a、CD11b及CD18均显著增高,结论提示高水平LDL似可上调单个核细胞表面与内皮细胞粘附有关的某些粘附分子表达而参与了动脉粥样硬化的发生发展。     

男性UAP患者血清雄激素与中性粒细胞表达 CD11b/CD18及其与细胞因子的关系

目的：探讨男性不稳定型心绞痛患者血清雄激素与中性粒细胞表达CD11b/CD18及其与细胞因子之间的关系。方法：选择80例男性不稳定型心绞痛患者（UAP组），60例冠状动脉造影阴性对象作对照（对照组），放射免疫法测定血清性激素，酶联免疫吸附法检测外周血白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、γ干扰素（IFN-γ），流式细胞仪检测中性粒细胞表达CD11b/CD18。结果：校正2组血压、体质量指数及血脂差异，UAP组血清游离睾酮显著低于对照组（P〈0．01）；IL-1β、IL-6、TNF-α、IFN-γ和中性粒细胞CD11b/CD18表达高于对照组（均P〈0．01）；2组血清总睾酮、去氢表雄酮、去氢表雄酮硫酸酯、雌二醇及总睾酮与雌二醇比值差别无统计学意义。UAP组游离睾酮与IL-1β、IL-6、TNF-α、IFN-γ和中性粒细胞表达CD11b/CD18呈负相关（均P〈0．01）；多元逐步回归分析表明，UAP组游离睾酮与中性粒细胞表达CD11b/CD18。独立负相关。结论：不稳定型心绞痛患者血清游离睾酮水平降低，可能与中性粒细胞表达CD11b／CD18上调和血清致炎细胞因子水平增高有关。     

腺苷与AMP579对冠心病患者中性粒细胞表面CD11b/CD18表达的影响

目的:探讨不同剂量腺苷与AMP579对不同类型冠心病(CHD)患者中性粒细胞表面CD11b/CD18表达的影响。方法:选择经临床症状、心电图改变及心肌酶学确诊的CHD患者及正常健康者采血,患者血液分别给予不同剂量的腺苷与AMP579进行干预,免疫荧光标记后用流式细胞仪分别检测正常人及患者用药前后中性粒细胞CD11b/CD18的表达值。结果:CHD患者的中性粒细胞CD11b/CD18的表达较正常人明显升高(P<0.01);腺苷在10,30,100μmol能有效降低CHD患者的中性粒细胞CD11b/CD18的表达(P<0.05),但30,100μmol组之间差异无显著性(P>0.05);AMP579组中,患者中性粒细胞CD11b/CD18的表达均未见明显降低,各组较对照组差异均无统计学意义(P>0.05)。结论:CHD的发生与CD11b/CD18表达升高有关;腺苷可通过降低CD11b/CD18的表达而降低白细胞与内皮细胞之间的黏附性;而AMP579在1,10,30μmol尚不能降低CHD患者CD11b/CD18的表达。     

一氧化碳中毒诱导迟发记忆障碍小鼠白细胞表面黏附分子CD_(11)b/CD_(18)表达的研究

目的通过对一氧化碳(CO)中毒诱导迟发记忆障碍小鼠白细胞表面黏附分子CD11b/CD18表达,探索CO中毒诱发的迟发脑病发病机制。方法筛选出急性CO中毒后诱发的迟发记忆障碍小鼠,测其血白细胞表面黏附分子CD11b/CD18表达情况。结果迟发记忆障碍小鼠白细胞表面黏附分子CD11b/CD18的表达量明显高于对照组。结论 CD11b/CD18高表达可能是迟发脑病的发病机制之一。     

茶多酚对肺癌PG细胞生长的调控研究

目的:探讨茶多酚对人PG细胞生长的影响。方法:采用MTT法体外观察不同浓度茶多酚对PG细胞的杀伤作用;应用激光共聚焦显微镜和流式细胞仪分析,检测用药后PG细胞内Ca2 +浓度、CD44 V6表达和细胞增殖周期分布的变化。结果:3个浓度的茶多酚对PG细胞均有杀伤作用,呈剂量依赖关系。细胞增殖受到明显抑制,使细胞阻滞于G0 / G1期,不能进入S期及G2 / M期,细胞增殖指数明显下降。细胞内Ca²⁺浓度与对照组相比,逐渐上升;CD44 V6表达水平则呈逐渐下降。结论:茶多酚对PG细胞的生长具有抑制作用,其作用机制与改变细胞内Ca2 +浓度和CD44 V6表达有关     

脑出血患者中性粒细胞表面粘附分子CD11b表达量的检测及临床意义

目的探讨脑出血患者中性粒细胞(polymorphonuclear neutrophil,PMN)表面粘附分子(CD11b)的表达量及其临床意义。方法用流式细胞术,以单克隆抗体为分子探针,对42例脑出血患者和20例健康对照者外周血中性粒细胞黏附分子CD11b的表达量进行检测(以平均荧光强度MFI来表示),观察脑出血患者72h内和第7天的CD11bMFI,并与健康人作对照。结果患者组72h内CD11b的表达量高于正常对照组(P<0.05);脑出血患者第7天CD11b的表达量较第3天为低(P<0.05),与正常对照组比较无差别(P>0.05)。神经功能缺损程度严重者,CD11b表达量多(r=0.35,P<0.05)。结论脑出血患者发病时,应激引起机体炎症反应系统活化;CD11b的表达量与脑出血的严重程度相关。     

水蛭素对凝血酶诱导血管内皮细胞与中性粒细胞表达黏附分子的影响

目的:研究水蛭素对凝血酶诱导人脐静脉内皮细胞(HUVEC)表达细胞黏附分子(ICAM-1)和中性粒细胞表达膜整合素CD11b的影响.方法:用酶消化法获得HUVEC,密度梯度离心和重力沉降法分离人外周血细胞中性粒细胞.用酶联免疫吸附测定HUVEC在凝血酶、水蛭素作用下ICAM-1的变化,同时以免疫荧光流式细胞术检测中性粒细胞表达CD11b水平的变化.结果:与对照组相比,凝血酶诱导的HUVEC 表达ICAM-1显著增多,随时间延长,ICAM-1的表达递增,高峰在24 h.凝血酶使中性粒细胞CD11b的表达也显著上调,6 h平均荧光强度明显增加,随时间延长逐渐升高,高峰在24 h.水蛭素和肝素对凝血酶诱导的HUVEC 表达ICAM-1和中性粒细胞表达CD11b均有明显的抑制作用,且水蛭素抑制ICAM-1的表达强于肝素,尤其在ICAM-1表达的高峰期,对CD11b表达的抑制作用水蛭素与肝素无明显差别.结论:水蛭素可以明显抑制凝血酶诱导的HUVEC 表达ICAM-1和中性粒细胞表达CD11b.     

硒化壳聚糖对HL60细胞增殖分化的影响

目的 探讨硒化壳聚糖与细胞c-myc和c-fos基因表达的关系及对人早幼粒白血病HL60细胞增殖和分化的影响.方法 采用SRB法和克隆形成法检测细胞增殖;Wright-Giemsa染色法检测细胞分化;流式细胞术检测CD11b、c-myc和c-fos分子表达.结果 25、50、100 mg/L硒化壳聚糖作用HL60细胞48 h可明显促进细胞分化和抑制细胞增殖(P＜0.05,P＜0.01);各浓度硒化壳聚糖均可使细胞CD11b蛋白表达显著升高(P＜0.01),c-myc蛋白表达下降(P＜0.05或P＜0.01),c-fos蛋白表达升高(P＜0.05或P＜0.01).结论 硒化壳聚糖可通过调控c-myc和c-fos基因表达来抑制HL60细胞增殖,促进细胞分化.     

Expression of inflammatory and apoptosis factors following coronary stent implantation in coronary heart disease patients

We investigated the changes in characteristics of neutrophil CD11b, monocyte CD11b, platelet CD62P, endothelin (ET), and neutrophil CD178 in patients with coronary heart disease (CHD) before and after primary coronary stenting. A total of 41 patients with CHD who underwent coronary stenting and 40 control subjects were enrolled in the study. In CHD patients, peripheral blood samples were taken 24 h before and 30 min, 24 h, and 72 h after successful coronary stenting. All markers were significantly elevated in patients with CHD compared with controls (P < 0.05). Time-course studies revealed that the expressions of neutrophil CD11b, monocyte CD11b, platelet CD62P, and ET were lower at 30 min post-operation (PO) compared with that at 24 h before operation (BO)(P < 0.05). All levels significantly increased from 30 min PO to 24 h PO (P < 0.05) and decreased thereafter until 72 h PO (P > 0.05). Time course changes in neutrophil CD11b levels after coronary stenting were significantly higher in patients with unstable angina pectoris than in patients with stable angina pectoris (P < 0.05). CD11b levels were related to CD62P in patients with CHD (P < 0.05). Neutrophil CD11b and monocyte CD11b levels were significantly increased in patients with CHD who underwent coronary stenting compared with controls (P < 0.05). Results show that CD11b levels increased, meanwhile, the levels of CD62P and ET increased in CHD patients after coronary stenting. In addition, neutrophil CD178 levels of apoptosis factor in patients, which is important for regression of inflammation, remained high for a period of time after coronary stenting.     

Differential effects of single dose FTY720 on CD62L+ B-cells in stable renal allograft recipients

FTY720, a sphingosine-1-phosphate receptor agonist, is the archeotype of a new class of immune modulators, which redirects lymphocytes from the peripheral blood into secondary lymphatic tissue. Previously, it was shown that FTY720 differentially decreases peripheral T-cells, expressing specific chemokine and adhesion receptors. Here, we investigated the effect of single doses FTY720 on peripheral B-cells expressing CD62L, CD11a, CD49d and CXCR4 in stable human renal allograft recipients. Peripheral blood lymphocytes were isolated by Ficoll density centrifugation and stained with monoclonal antibodies against CD3 or CD19 and CD62L, CD11a, CD49d, CXCR4 to determine the percentage of these T- and B-cell subpopulations. Total lymphocyte counts were measured by routine laboratory diagnostics to calculate absolute lymphocyte subset counts. In FTY720 treated patients, total lymphocyte counts decreased by 31.8% (0.25-2 mg) and 60.4% (3.5 mg), and total T-cell counts by 38.8% (0.25-2 mg) and 70.9% (3.5 mg). In comparison, total B-cell counts decreased by 32.2% (0.25-2 mg) and 61.1% (3.5 mg). The reduction of CD62L+ B-cells was less pronounced as compared to CD62L+ T-cells (0.25-2 mg: 15.7% vs. 57.3%; 3.5 mg: 57.2% vs. 86.9%). CD11a+ B-cells decreased by 15.4% (0.25-2 mg) and 57.1% (3.5 mg), and CD49d+ B-cells by 15.0% (0.25-2 mg) and 56.7% (3.5 mg). CXCR4+ B-cells decreased by 19.9% (0.25-2 mg) and 57.2% (3.5 mg). In vitro experiments showed that FTY720 did not change the mean expression of CD62L, CD11a, CD49d and CXCR4 on CD19+ B-cells. In conclusion FTY720 treatment reduces B-cells expressing CD62L to a significant lesser degree than T-cells expressing CD62L.     

Adhesion molecules of blood polymorphonuclear leukocytes and alveolar macrophages in rats: modulation by exposure to ozone.

1. Exposure to elevated levels of ozone results in an infiltration of polymorphonuclear leukocytes (PMNs) into the lungs. The purpose of this study was to investigate whether the ozone-induced inflammatory process is preceded by a change in the expression of adhesion molecules (integrins and selectins) in peripheral blood PMNs and alveolar macrophages in rats. 2. Female Sprague Dawley rats were exposed to air or ozone (1 p.p.m., 2 h). Bronchoalveolar lavage (BAL) was carried out and blood was collected via intracardiac puncture at 0 or 18 h after the exposure. There were no PMN in the BAL fluid at time 0 after the 2 h exposure to ozone. The expression of cell adhesion molecules from the integrin family (represented by CD18) on alveolar macrophages (AM) was lowered. The expression of cell adhesion molecules from the selectin family (represented by CD62L) on blood PMN was not affected by exposure to ozone, while the expression of integrins (CD11b) on blood PMN was lowered. 3. This effect was confirmed by experiments in which plasma of ozone-exposed animals was incubated with PMN from peripheral blood obtained from non-exposed animals. In these experiments, the expression of CD11b on PMNs of non-exposed animals was lower after incubation with plasma from ozone-exposed animals. 4. Our experiments suggest the presence of factor(s) in blood, which cause a decrease in the expression of CD11b on PMNs.     

老年恶性肿瘤患者红细胞免疫功能、T细胞亚群和可溶性白介素—2受体的研究

本文检测了52例老年恶性肿瘤患者红细胞免疫功能、T淋巴细胞亚群和血清可溶性白介素 2受体(SIL 2R)水平的变化。结果显示肿瘤患者红细胞C_3b受体花环率(EC_3bRR)较对照组明显降低(P<0.001),而免疫复合物花环率明显升高。CD_4、CD_4/CD_8比值降低,CD_8和SIL 2R增高,与对照组相比,差异有显著性意义(P<0.001)。E c_3bRR与CD_4、CD_4/CD_8呈正相关,与CD_8、SIL 2R呈负相关。结果提示老年恶性肿瘤患者与红细胞免疫功能紊乱和T细胞亚群、血清SIL 2R水平异常有密切关系。     

流式细胞术预测冠心病患者阿司匹林抵抗的研究

目的 利用流式细胞术制定阿司匹林抵抗(AR)的诊断标准,预测冠心病患者阿司匹林抵抗的发生率.方法 采用流式细胞术测定127例冠心病(阿司匹林组103例和空白对照组24例)患者人院时及经治疗7 d后P选择素(CD62P)和纤维蛋白原受体(PAC-1)的表达率.利用ROC曲线法设定AR诊断标准,统计AR在住院冠心病患者中的发生率.结果 阿司匹林组治疗前CD62P(10.16±6.80)%、PAC-1(14.66±10.56)%,治疗后CD62P(5.70±4.28)%、PAC-1(8.93±7.08)%;空白对照组治疗前CD62P(9.14±6.52)%、PAC-1(17.67±11.53)%,治疗后CD62P(7.81±5.72)%、PAG-1(14.97±8.05)%,差异有统计学意义(P＜0.05).根据ROC曲线设定CD62P抑制率＜21.5%或PAC-1抑制率＜17.7%为诊断AR标准,AR在住院冠心病患者中的发生率为17.5%.结论 CD62P抑制率＜21.5%或PAC-1抑制率＜17.7%为AR诊断标准.AR发生率为17.5%.     

灯盏细辛注射液对急性脑梗死患者血清粘附分子sICAM-1和CD11b/CD18的影响

目的 观察灯盏细辛注射液对急性脑梗死(acute cerebral infaction,ACI)患者的临床疗效及对血清粘附分子sICAM-1和CD11b/CD18表达的对影响.方法 69例ACI患者随机分为灯盏细辛治疗组(36例)和常规治疗组(33例),采用双抗体夹心ELISA法、流式细胞仪(FCM)检测两组ACI患者治疗前后血清粘附分子sICAM-1和CD11b/CD18表达的水平.结果 ACI患者治疗前血清粘附分子sICAM-1和CD11b/CD18的表达水平显著高于健康人组(p＜0.05),灯盏细辛治疗组临床有效率明显高于常规治疗组(P＜0.05),两组治疗后血清粘附分子sICAM-1和CD11b/CD18的表达均明显下降,灯盏细辛治疗组降低较常规治疗组更明显(p＜0.05).结论 灯盏细辛注射液治疗ACI可能与其下调血清粘附分子sICAM-1和CD11b/CD18的表达,减轻中枢神经系统的炎性损伤密切相关.     

Platelet activation, upregulation of CD11b/ CD18 expression on leukocytes and increase in circulating leukocyte-platelet aggregates in Indian women chronically exposed to biomass smoke

The majority of households in rural India still rely on unprocessed solid biomass for domestic energy. The aim of this study was to investigate whether chronic exposure to biomass smoke causes activation of leukocytes and the formation of leukocyte-platelet aggregates. We conducted flow cytometric analysis of beta2 Mac-1 integrin (CD11b/CD18) expression on polymorphonuclear leukocytes (PMN) and monocytes, and P-selectin (CD62P) expression on the platelets of 165 women from eastern India, who cook solely with wood, dung and agricultural wastes, and 155 age- and socio-economic condition-matched control subjects, who used relatively cleaner fuel, liquefied petroleum gas (LPG). Leukocyte-platelet aggregates were defined as CD11b-positive PMN and monocytes co-expressing platelet-specific markers CD41 or CD62P. A significant increase in leukocyte-platelet aggregates was found in women who used biomass as cooking fuel. In addition, they showed increased surface expression of CD11b/CD18 in circulating PMN and monocytes and CD62P expression on platelets. The mean fluorescence intensity (MFI) of CD11b on the surface of circulating monocytes and PMN of biomass users increased by 50 and 68%, respectively. Similarly, a 62 and 48% increase in MFI was observed in CD18 expression on the surface of these cells in biomass users. The results show that chronic biomass smoke exposure activates circulating platelets, PMN and monocytes, and increases the number of leukocyte-platelet aggregates, which are considered a risk factor for thrombosis.