人参及人参皂甙对荷瘤小鼠血清中表皮生长因子含量的影响

给Ｃ５７ＢＬ／６小鼠移植Ｌｅｗｉｓ肺癌细胞（２×１０＾７）２周后，以放射免疫法测定其血清中表皮生长因子（ＥＧＦ）含量，肿瘤组为０．２８±０．０９μｇ／Ｌ，正常鼠组为０．１８±０．１１μｇ／Ｌ，移植肿瘤后服用人参煎剂组（人参组）为０．１６±０．１０μｇ／Ｌ，移植肿瘤后服用人参皂甙组（人参皂甙组）为０．２９±０．１１μｇ／Ｌ。肿瘤组鼠的ＥＧＦ含量明显高于正常鼠，人参组明显低于肿瘤组鼠，人参皂甙组与肿瘤     

某市儿童铜锌营养状况的分析与评价

采用溶出分析法对济宁市市区内２８８名２－８岁儿童血清Ｃｕ、Ｚｎ及头发Ｃｕ、Ｚｎ含量进行了测定，结果血清Ｃｕ、Ｚｎ含量均分别为１８．０±４．０μｍｏｌ／Ｌ、１２．７±２５μｍｏｌ／Ｌ，头发Ｃｕ、Ｚｎ含量均值分别为１１．５±３．２μｇ／ｇ、１０３．６±２５．６μｇ／ｇ血清Ｃｕ与发Ｃｕ、血清Ｚｎ与发Ｚｎ之间无明显的直线相关关系，但以献提供的血清及头发Ｃｕ、Ｚｎ正常界值评价机体Ｃｕ、Ｚｎ营养状况，结果一     

鼻咽癌患者血清唾液酸含量的观察

测定２１例初诊鼻咽癌及３５例对照组血清唾液酸含量。结果初诊鼻咽癌息者血清唾液酸平均值为０．８１±０．５７ｇ／Ｌ，显著高于正常对照组０．３４±０．１４ｇ／Ｌ（Ｐ＜０．０１）；放疗后未复发鼻咽癌１０例血清唾液酸平均值为０．３７±０．３３ｇ／Ｌ。另６例放疗后复发鼻咽癌患者血清唾液酸含量为２．２７±１．２２ｇ／Ｌ。结果提示鼻咽癌患者血清唾液酸水平与患者的病情有关。     

微血管病变患者血清学检测的临床意义

目的：了解微血管病变患血清洋地黄样物质（ＥＤＬＳ）与超氧化物歧化酶（ＳＯＤ）的关系及其意义。方法：应用放射免疫法检测微血管病变患血清ＥＤＬＳ、ＳＯＤ含量，酶法检测血清甘油三酯、胆固醇水平。结果：１５例正常对照血清ＥＤＬＳ、ＳＯＤ浓度为０．３９±０．０７μｇ／Ｌ、４２２±１２７μｇ／Ｌ，２８例糖尿病患、１８例脑出血患、２３例脑梗塞患血清ＥＤＬＳ浓度分别为０．５５±０．１４μｇ／Ｌ、０．６     

小儿感染性疾病血清纤维结合蛋白测定的临床价值

本文测定了４５例感染儿和２０例健康儿血清纤维结合蛋白（Ｆｎ）、转铁蛋白（Ｔｆ）、免疫球蛋白（Ｉｇ）含量。结果：①感染儿Ｆｎ（０．０３８±０．０３５ｇ／Ｌ）明显低于健康儿（０．１８８±０．０３７ｇ／Ｌ）（Ｐ＜０．００１），②４５例感染儿中２５例肺炎与２０例非呼吸道疾病儿Ｆｎ均低于健康组（分别为０．０３３±０．０２９ｇ／Ｌ和０．０４４±０．０３ｇ／Ｌ），Ｐ均＜０．００１）。２５例肺炎儿中１２例重型Ｆｎ（０．０１９０±０．０１５６ｇ／Ｌ）较普通型（０．０３８±０．０３１ｇ／Ｌ）降低（Ｐ＜０．０５）③感染儿Ｔｆ（１．２４７±０．８５４ｇ／Ｌ）明显低于健康儿（１．９９９±０．２５１ｇ／Ｌ）（Ｐ＜０．００１），感染儿ＩｇＧ（９．５９３±３．０４１ｇ／Ｌ）也明显低于健康儿（１３．０１０±０．４７４ｇ／Ｌ）（Ｐ＜０．００１）；ＩｇＡ、ＩｇＭ无显著差异。表明：①Ｆｎ含量可作为揭示感染及判定感染严重程度的客观指标。②Ｔｆ值具有提示感染及评估铁营养状况的双重价值。③本实验在临床上对感染性疾病患儿评价病情及指导治疗均有实用意义     

肝硬化患者血清Ⅲ型前胶原水平及其与HBVe抗原抗体的关系

目的：了解肝硬化患者血清Ⅲ型前胶原（ＰＣⅢ）水平及其与ｅ抗原抗体的关系。方法：采用放免法检测７１例慢性乙型肝病患者（慢迁肝３４例，肝硬化３７例）血清ＰＣⅢ水平。结果：慢迁肝组血清ＰＣⅢ水平为１１６．７±２０．５μｇ／Ｌ，与对照组比较无显著性差异（Ｐ＞０．０５）；肝硬化组血清ＰＣⅢ水平为１５８．６±４２．６μｇ／Ｌ，与对照组比较差异显著（Ｐ＜０．０５）。肝硬化组中，ＨＢｅ－Ａｇ阳性亚组和抗ＨＢｅ阳性亚组血清ＰＣⅢ分别是１３３．９±２０．２μｇ／Ｌ和１７２．９±５２．４μｇ／Ｌ，二者比较有显著差异（Ｐ＜０．０５）。结论：血清ＰＣⅢ水平检测对肝硬化有一定诊断价值；临床应重视抗ＨＢｅ阳性的慢性肝病患者肝纤维化的倾向     

老年肝病患者血清透明质酸检测的临床意义

对８６例老年肝病患者血清透明质酸（ＨＡ）的变化进行观察表明，肝硬变患者ＨＡ水平最高，平均８５６．３４±３８６．９７μｇ／Ｌ，其次为急性黄疸型肝炎（６２０．３６±４５６．８７μｇ／Ｌ）、慢性活动肝炎（３１４．４３±２８６．２４μｇ／Ｌ），以上三组均较正常人（５７．８３±２１．７０μｇ／Ｌ）明显升高（Ｐ＜０．０５及０．０１）。早期肝硬变较慢性活动性肝炎明显升高，对诊断有帮助，ＨＡ升高与Ⅲ型前胶原肽和总     

眼部恶性肿瘤患者泪液和血清β2—微球蛋白,癌胚抗原的含量测定

目的探索一种对眼部恶性肿瘤较可靠实用的诊断和随诊方法。方法对眼部恶性肿瘤患者４７例用ＲＩＡ法测定了泪液和血清β２－微球蛋白（β２－Ｍ）、癌胚抗原（ＣＥＡ）的含量，以良性肿瘤４４例和正常人５０例对照。结果恶性肿瘤β２－Ｍ含量，泪液１４．６１±５．５７ｍｇ／Ｌ、血清１．５４±１．０８ｍｇ／Ｌ，另两组泪液β２－Ｍ含量均值分别为８．６０±２．６２ｍｇ／Ｌ，８．５４±１．４０ｍｇ／Ｌ．升高阳性率泪液７１．１１％、血清９．５２％．恶性肿瘤组ＣＥＡ含量，泪液８３．２４±４７．２６μｇ／Ｌ，血清１１．１１±５．２５μｇ／Ｌ，另两组泪液ＣＥＡ含量分别为２４．２６±９．９２μｇ／Ｌ，２０．１７±３．４６μｇ／Ｌ．升高阳性率泪液９５．３５％、血清４．７６％．泪液β２－Ｍ和ＣＥＡ值均升高的阳性率６９．０５％．恶性肿瘤组与另两组比较，差异非常显著（Ｐ＜０．０１），升高阳性率也明显高于血清。术后观察无复性者泪液两项值下降至正常，有复发者升高。结论泪液和血清β２－Μ，ＣＥＡ检测并结合临床可作为眼部恶性肿瘤的一项较可靠的辅助诊断指标和随诊方法，有较大的临床应用价值。     

不孕症妇女血清催乳素含量的测定

采用放射免疫法（ＲＩＡ）测定１８６例不孕症妇女血清催乳素（ＰＲＬ）浓度。结果表明，高催乳素血症１１５例，占６１．８２％。其中月经周期正常６１例，ＰＲＬ均值２８．３７±１６．７８μｇ／Ｌ；月经周期不规则６３例，ＰＲＬ均值５４．００±４７．５５μｇ／Ｌ；闭经６２例，ＰＲＬ均值为１７１．０３±１３３．３４μｇ／Ｌ．经统计学处理，３组血清ＰＲＬ值差异有极显著性（Ｐ＜０．００１），且ＰＲＬ＞２００μｇ／Ｌ者２７例，全部出现闭经，１７例确诊为垂体瘤。提示血清ＰＲＬ含量与女性生殖内分泌有密切关系。ＰＲＬ增高影响月经周期变化，导致排卵障碍、黄体功能不全并不孕。     

脐血必需元素含量与胎儿畸形的相关性研究

应用偏振塞曼原子吸收法测试１５５例脐血必需元素的含量。结果畸表儿组脐血锰（２４．５５±７．３４）μｇ／Ｌ；铁（３２８．５８±１１８．５０）ｍｇ／Ｌ；铜（０．６９±０．２９）ｍｇ／Ｌ；镁（３３．８９±８．８６）ｍｇ／Ｌ，正常体重儿组脐血锰（３１．１２±１１．６７）μｇ／Ｌ；铁（３７３．６８±９７．８０）ｍｇ／Ｌ；铜（０．５７±０．２０）ｍｇ／Ｌ；镁（３０．９２±６．５９）ｍｇ／Ｌ．畸形儿组脐血锰、铁含量明显低于正常体重儿组（Ｐ＜０．０１及Ｐ＜０．０５），而铜、镁含量高于正常体重儿组（Ｐ＜０．０１及Ｐ＜０．０５）。提示低锰、低铁与胎儿畸形关系非常密切，多种人体必需元素平衡失调与胎儿畸形有关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.     

Notes for contributors to Journal of Nanjing Medical University

Journal of Nanjing Medical University (English Edition) JNMU, sponsored by Nanjing Medical University, was established in 1987. It is a bimonthly comprehensive English medical journal published locally and abroad.Since 2007, Journal of Nanjing Medical University (English Edition )was granted Elsevier the full publishing and distribution rights worldwide for the Electronic Edition, excluding the People's Republic of China.