不同剂量丹参治疗银屑病患者后血液流变学改变

我们对本院近年来收治的 48例寻常型银屑病患者进行了血液流变学指标检测,现将结果报道如下。 一、病例和方法 (一 )检测对象： 48例住院的寻常型银屑病患者,居住地海拔 2 260～ 4 000 m。世居或移居半年以上。观察组男 27例,女 21例;年龄 9～ 66岁,平均 30.5岁;病史 7 d至 28年。进行期 20例,静止期 28例;点滴状 18例,斑块状 20例,混合性 10例。皮损面积 < 10％ 9例, 11％～ 40％ 31例, >40％ 8例。对照组 48例,居住地同观察组,男 29例,女 19例;年龄 10～ 68岁,平均 31.3岁。经全面体检、 X线胸透、心电图等检查,排除系…     

狼毒治疗银屑病的疗效观察及血液流变学检测

1994年3月以来我们用狼毒粉治疗寻常性银屑病近1000例,取得较满意疗效。为进一步探讨狼毒的作用机理,对其中35例治疗前后进行了血液流变学检测,并与35例口服制银灵片作对照,现将观察结果报告如下。一般资料　70例均为银屑病专科门诊患者。治疗组35例,男20例,女15例,年龄16～67岁,平均36.5岁,病程3月～31年,平均8.5年,进行期27例,静止期8例。对照组35例,男19例,女16例,年龄12～68岁,平均35岁,病程2月～30年,平均9.2年,进行期25例,静止期10例。治疗方法　治疗组:将狼毒(大戟科植物月腺大戟干品,EuphorbiaebiacteolataHayata)加工成粉剂,装入…     

银屑病患者血栓调节蛋白及血液流变学检测

血栓调节蛋白是存在于血管内皮细胞膜上的糖蛋白,在血管内凝血的调节中起重要的作用。已证实血浆可溶性血栓调节蛋白 (sTM)增高是血管内皮细胞受损的标志 [1]。 Lowe等 [2]的研究表明银屑病患者存在微血管异常,并易合并闭塞性血管疾患 [3]。我们检测了银屑病患者血浆 sTM及血液流变学指标,旨在探讨血管内皮受损和血液流变学异常与银屑病的发病及其合并症的关系。 一、资料和方法 (一 )研究对象：患者组：寻常型银屑病患者 38例,男 29例,女 9例;年龄 10～ 69岁,平均 (49.2± 16.6)岁;病程 1个月至 40年,其中进行期 26例,静止期…     

红皮病型银屑病血液流变学改变及临床意义

探讨红皮病型银屑病患者血液流变学改变和临床意义.检测49例红皮病型银屑病患者全血粘度、血浆粘度、红细胞聚集指数、红细胞刚性指数等血流变指标,并与50例寻常型银屑病患者(进行期)和健康对照组做比较.结果红皮病型银屑病患者的全血粘度、血浆粘度、红细胞聚集指数、红细胞刚性指数等血流变指标与寻常型银屑病和健康对照组相比显著增高.红皮病型银屑病患者存在明显的血液流变学改变,需在治疗中给予重视.     

活血化瘀法治疗银屑病疗效与血液流变学变化的关系

银屑病治疗较为棘手,尤其是寻常性静止期斑块状银屑病顽固难愈.笔者采用活血化瘀方法治疗90例寻常性银屑病患者,同时在治疗前、后进行血液流变学指标的检测,探讨其治疗效果与血液流变学变化之间的关系,以期为临床应用传统治疗方法提供现代医学的理论依据.     

银屑病及荨麻疹血液流变学的临床初步观察

本文对29例银屑病和荨麻疹患者进行了血液流变学观察,并与30例健康人进行了对比,结果银屑病者红细胞电泳时间与正常人比较有极显著差异(P<0.01);荨麻疹患者红细胞电泳时间及纤维蛋白原量较正常人均有极显著差异(P<0.01),血浆还原比粘度亦有显著性差异(P<0.05)。而血球压积、血浆比粘度、血沉及血沉K值均无显著性差异。     

银屑病患者甲皱微循环及其与血液流变学变化关系的研究

银屑病患者有明显的微循环障碍,全血还原粘度、血浆粘度和血小板聚集性增高。微循环障碍和血液流变学及血小板聚集性变化有一定关系。提示本病可采用调整微循环功能,降低血液粘度和减少血小板聚集性的治疗方法来替代副作用大且有致癌危险性的细胞性药物。     

银屑病中医分型的血液流变学及超微结构相关性研究

目的 探讨银屑病中医分型与血液流变学及超微结构改变的相关性。方法 将35例寻常性悄病按中医辩证分型,同步进行动态血液流变学6项指标及超微结构检测。结果 血热型6项指标无明显变化,真皮毛细血管腔变大;血瘀型血液粘度、红细胞压积增主,红细胞电泳时间延长,毛细血管管腔狭窄;血燥型血沉增快,毛细血管减少。结论 银屑病真皮毛细血管及血液流变学改变具有一定的相关性,提示微循环障碍与银屑病发病有关。并为中医辩证分型提供了客观依据。     

Low density lipoprotein receptor expression on keratinocytes in normal and psoriatic epidermis

Biochemical and morphologic studies on the interaction of low density lipoprotein (LDL) with cultured normal keratinocytes and squamous carcinoma cells have shown a negative correlation between LDL receptor activity and terminal differentiation of the epidermal cells [Ponec M et al, J Invest Dermatol 83:436-440, 1984 and Vermeer, BJ et al, J Invest Dermatol 86:195-200, 1986]. Whether such in vitro studies pertain to the epidermis in vivo is not known. To obtain information on the distribution of LDL receptors in the epidermis in situ, morphologic studies were performed using LDL-gold as an ultrastructural marker. When freshly isolated mouse and human epidermal cells were incubated with LDL-gold complexes, only keratinocytes with the morphologic characteristics of basal cells showed binding and uptake of LDL-gold. No LDL receptor activity was found on Langerhans cells, melanocytes or highly differentiated keratinocytes. Since cell separation techniques can destroy receptors, the staphylococcal epidermolytic toxin was utilized to produce intercellular and intra-epithelial splitting of the epidermis. In preparations of both normal mouse and human epidermis, LDL-gold binding was restricted to basal cells and a few suprabasal keratinocytes. In contrast, in psoriatic epidermis, and to a lesser extent, essential fatty acid-deficient mouse epidermis, cells in the stratum spinosum showed abundant LDL-gold binding. Thus LDL-gold may be a useful marker for epidermal differentiation.     

Differences in low density lipoprotein receptor expression in the suprabasal layer of normal and psoriatic epidermis

Previous morphological experiments on the distribution of binding sites for low density lipoprotein (LDL) on normal and psoriatic epidermis in situ, done with the LDL-gold technique [Mommaas-Kienhuis AM, et al. J Invest Dermatol 89: 513-517, 1987.] showed an unequivocal correlation between the ability to bind LDL-gold complexes and the state of keratinocyte differentiation. To determine the involvement of the LDL receptor in this phenomenon, we applied immunoelectronmicroscopical methods in conjunction with a monoclonal anti-LDL receptor antibody. Biopsy specimens of normal and psoriasis skin were fixed before being embedded in Lowicryl K4M. Ultrathin sections were incubated first with the anti-LDL receptor antibody, and then with a second antibody conjugated to colloidal gold. On basal cells of both normal and psoriatic epidermis the LDL receptor was distributed evenly between the cell surface and the cytoplasm. No obvious differences in the density of LDL receptors were observed. However, cells from the suprabasal layer showed two striking differences in the localization of the LDL receptor: 1) normal epidermis showed fewer LDL receptor molecules, whereas in psoriasis epidermis the number increased relative to those on basal cells; and 2) in normal suprabasal cells most of the LDL receptors were located inside the cell, but in psoriasis the majority was found on the cell surface. Both phenomena are discussed and we postulate that the higher expression of LDL receptors in psoriasis suprabasal cells and the high expression of the receptor on the cell surface is connected with the hyperproliferative state of the disorder.     

Detection of leukotrienes in the serum of asthmatic and psoriatic patients

Purified serum samples from asthmatic and psoriatic patients and healthy controls were analysed by high-pressure liquid chromatography (HPLC) and the amounts of leukotrienes were measured from the corresponding HPLC fractions by specific radioimmunoassays. In the serum of healthy controls the amounts of leukotrienes B4, C4 and D4 were very small or negligible. Rather great amount of leukotriene B4 was, however, detected in the serum of many asthmatic and psoriatic patients. The amount of leukotriene B4 was in the serum of asthmatic patients 120 +/- 20 pmol/ml (n = 11, mean +/- SEM) and in that of psoriatic patients 100 +/- 10 pmol/ml (n = 10). The amounts of leukotrienes C4 and D4 were rather small in the serum of most patients. The amount of leukotriene C4 was, however, very high (250 pmol/ml) in the serum of a psoriatic patient. Significant amount of leukotriene D4 was also detected in the serum of this patient. The present study indicated that leukotrienes are formed during blood clotting in the leukocytes of asthmatic and psoriatic patients and that the rate of formation is so high that leukotrienes may have a role in these diseases.     

Soluble E-selectin serum levels correlate with disease activity in psoriatic patients

E-selectin is an adhesion molecule expressed on vascular endothelial cells in several inflammatory skin diseases, including psoriasis. It is responsible for the adherence between microvascular endothelium and neutrophils, monocytes, eosinophils and subsets of T cells. Soluble E-selectin (sE-selectin) serum levels were measured by ELISA in 32 psoriatic patients before treatment and compared with both post-treatment sE-selectin levels in 16 patients and sE-selectin values in 10 healthy individuals. Soluble E-selectin serum levels were significantly increased in psoriatic patients compared with healthy persons. Moreover, a significant correlation was demonstrated between sE-selectin values and PASI scores. No relationship was found between sE-selectin levels and duration of psoriasis. Soluble E-selectin serum levels decreased significantly after treatment of psoriasis. This phenomenon was more evident in patients with more severe psoriasis. In conclusion, sE-selectin serum levels correlate with the extent of psoriatic lesions and could be used as marker of the disease activity in psoriatic patients.     

Estimation of high density lipoprotein cholesterol in the diagnosis of lepromatous leprosy

A high incidence of increased plasma level of high density lipoprotein cholesterol (HDL-C) has been reported in cases of lepromatous leprosy. HDL-C levels were estimated in 96 (50 under treatment and 46 untreated) lepromatous leprosy patients and 84 randomly selected matched control patients suffering from other skin diseases attending skin out-patients department. HDL-C estimations were performed for the diagnosis of lepromatous leprosy in patients aged below 60 years, taking plasma HDL-C levels as 28-71 mg./dl. in men and 34-91 mg./dl. in women, as range of normal values. The study revealed that HDL-C levels in lepromatous leprosy group were raised and significantly different when compared with control group (t = 35.1668 and P less than 0.001). The sensitivity of the test was very high, 97.9 per cent (94/96), but specificity was low 80.95 per cent (68/84). False positive and false negative results were 19.04 per cent (16/84) and 2.08 per cent (2/96) respectively. It is opined that a negative test will be mainly useful in excluding diagnosis of lepromatous leprosy.     

MTX对银屑病患者血清白介素-8和组胺水平的影响

目的:探讨甲氨蝶呤(MTX)对银屑病患者血清白介素-8和组胺水平的影响。方法:用酶联免疫吸附实验(ELISA)检测银屑病患者和正常人外周血IL-8水平;用荧光分光光度计测定银屑病患者和正常人外周血组胺水平。结果:(1)治疗前银屑病患者血清IL-8和组胺水平明显高于正常对照组(P<0.01),经MTX治疗后,患者血清IL-8和组胺水平与正常对照组差异无显著性(P>0.05)。结论:抑制组胺的释放和IL-8的分泌,降低中性粒细胞和T淋巴细胞的趋化活性,减轻炎症反应及组织损伤可能是MTX治疗银屑病的作用机制之一。     

阿维A对银屑病患者血清血小板衍生生长因子-BB和干扰素诱导蛋白-10的影响

目的:了解阿维A对银屑病患者血清中某些细胞因子水平的影响.方法:采用酶联免疫分析法检测血清血小板衍生生长因子-BB(PDGF-BB)和干扰素诱导蛋白-10(IP-10)水平.结果:银屑病患者在治疗前血清PDGF-BB和IP-10水平均比对照组高(P＜0.01).阿维A治疗后血清PDGF-BB和IP-10水平均显著下降(P<0.01).结论:阿维A对银屑病患者血清中PDGF-BB和IP-10的产生有显著抑制作用,为其治疗银屑病提供理论基础.     

Neurotransmitter imbalance in serum of psoriatic patients in exacerbation stage with comorbid psychoemotional disorders

The role of psychological factors in the manifestation and exacerbation of psoriasis is well known. Acute and chronic stress, anxiety, and depression affect the reactions of innate and acquired immunity. The exacerbations of psoriatic lesions are accompanied by increased production of inflammatory mediators, which could contribute to the imbalance of neurotransmitters and the development of symptoms of depression and anxiety. The role of inhibitory neurotransmitters in the serum of patients with psoriasis exacerbation with comorbid emotional disorders is assessed. Forty patients with psoriasis in the exacerbation stage were examined. Spielberger State‐Trait Anxiety Inventory (STAI) and the Beck Depression Inventory (BDI) scale were used to assess anxiety and depression parameters. We also evaluated the concentration of serotonin and gamma‐aminobutyric acid (GABA) in the serum. Patients with psoriasis had significant anxiety and depressive disorders. An imbalance in serotonin levels and a decrease in serum GABA levels in patients with psoriasis in the acute stage were noted. Only patients with anxiety disorder had increased serotonin levels. Our findings suggest that the imbalance of neurotransmitters in patients with psoriasis in the acute stage is important in predicting the development of the emotional well‐being.