高糖高脂饲料对Wistar大鼠生长和糖脂代谢的影响

目的观察高糖高脂饲料对Wistar大鼠生长及糖脂代谢的影响。方法雄性Wistar大鼠20只,按体重和空腹血糖随机分为2组(n=10):对照组和实验组,分别以普通饲料和高糖高脂饲料喂养6周,观察大鼠的生长曲线、食物利用率,测定大鼠的糖脂代谢指标。结果喂养6周后,实验组大鼠的体重、食物利用率、内脏脂肪占体重百分比、血清总胆固醇和低密度脂蛋白胆固醇均显著高于对照组(P<0.05),血清高密度脂蛋白胆固醇显著低于对照组(P<0.01)。两组的空腹血糖(FPG)无显著差异。实验组大鼠的糖耐量降低,葡萄糖曲线下面积显著高于对照组。结论正常血糖状态下,高糖高脂饲料可引起Wistar大鼠中心型积聚的体脂增加,血清总胆固醇增加,导致葡萄糖耐量能力减退,为2型糖尿病进程中的重要危险因素。     

麦麸超细粉的减肥降脂作用研究

目的观察麦麸超细粉的减肥和辅助降血脂作用。方法雄性SD大鼠适应性喂养后按体重随机分为空白对照组、模型对照组、200目麦麸超细粉组和100目麦麸超细粉组。空白对照组动物喂饲普通饲料、以蒸馏水灌胃,模型对照组动物喂饲高脂饲料(70%普通饲料+15%猪油+15%蔗糖)、以蒸馏水灌胃,实验组动物喂饲高脂饲料并以5g/kg.bw麦麸超细粉灌胃,连续42d。第43天动物股动脉取血,测血清总胆固醇、甘油三酯、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇。取肾周围和睾丸周围体脂,称重,计算脂/体比。结果实验结束时,模型对照组大鼠体脂肪重量(15.1±6.3)g和脂/体比(3.5±1.1)%高于空白对照组(P0.05),200目麦麸超细粉组大鼠体重(369.9±54.5)g、体脂肪重量(9.4±4.2)g和脂/体比(2.5±0.9)%低于模型对照组(P0.05)。实验结束时,模型对照组大鼠血清胆固醇(2.8±0.43)mmol/L、甘油三酯(4.6±1.52)mmol/L和低密度脂蛋白胆固醇(2.12±0.70)mmol/L高于空白对照组(P0.05),200目麦麸超细粉组大鼠血清甘油三酯(2.5±0.68)mmol/L和低密度脂蛋白胆固醇(1.13±0.31)mmol/L低于模型对照组(P0.05)。结论200目麦麸超细粉样品具有减肥和辅助降血脂的作用,超微粉碎技术可望成为麦麸深加工的有效手段。     

白藜芦醇对C57BL/6J小鼠脂代谢的影响

目的观察22.5mg/kg BW白藜芦醇对C57BL/6J小鼠血清和肝脏脂质的影响。方法 30只C57BL/6J雄性小鼠随机分为正常对照组、高脂组和高脂+白藜芦醇组,每组10只,正常对照组喂饲基础饲料,高脂组和高脂+白藜芦醇组喂饲高脂高胆固醇饲料。高脂+白藜芦醇组每日经灌胃给予22.5mg/kg BW白藜芦醇羧甲基纤维素钠溶液进行干预,正常对照组和高脂组均给予0.5%的羧甲基纤维素钠溶液灌胃。干预8周后测定小鼠血清TC、TG、HDL-C和LDL-C水平,肝脏TC和TG水平,并观察小鼠肝组织病理改变。结果高脂组和高脂+白藜芦醇组小鼠血清TC、LDL-C和HDL-C水平均高于正常对照组(P<0.05),高脂组血清TC和LDL-C水平还高于高脂+白藜芦醇组(P<0.05),但高脂组和高脂+白藜芦醇组小鼠血清TG水平均低于正常对照组(P<0.05)。高脂组小鼠肝脏TC水平高于高脂+白藜芦醇组和正常对照组(P<0.05)。结论 22.5mg/kg BW白藜芦醇可以降低喂饲高脂饲料的C57BL/6J小鼠体内胆固醇水平。     

C-反应蛋白与肥胖大鼠胰岛素抵抗相关性

目的探讨饮食诱导肥胖大鼠血清C-反应蛋白(CRP)与胰岛素抵抗(IR)的关系。方法30只雄性Wistar大鼠随机分成对照组和高脂组,分别喂基础饲料和高脂饲料12周,测定12周末空腹血糖(FPG)、空腹胰岛素(FINS)、血清CRP、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平,以胰岛素敏感指数(ISI)判定机体胰岛素抵抗(IR)状态。结果12周末,高脂组大鼠血清CRP、TNF-αI、L-6水平分别为(2.933±1.039)mg/L,(2.029±0.152)μg/L,(130.32±23.28)ng/L,均高于对照组(P<0.05),而且TNF-αI、L-6分别与CRP呈正相关(P<0.05),CRP与ISI呈负相关(P<0.05)。结论CRP可能涉及肥胖及肥胖相关的IR。     

高脂饮食大鼠脂肪组织SOCS-3及FAS表达

目的 研究高脂饮食诱导产生的肥胖及肥胖抵抗大鼠脂肪组织细胞因子信号转导抑制因子-3(SOCS-3)及脂肪酸合成酶(FAS)的mRNA表达情况.方法 Wistar雄性大鼠31只,其中7只喂饲普通基础饲料作为对照组;24只喂饲高脂饲料,第8周末,按体重增量从高脂饲料组筛选出5只大鼠作为肥胖组,5只大鼠作为肥胖抵抗组.测定血清甘油三酯和总胆固醇,检测附睾脂肪组织SOCS-3及FAS的mRNA表达.结果 肥胖组大鼠血清甘油三酯及总胆固醇水平分别为(0.982±0.228),(2.213±0.364)mmol/L,均显著高于对照组大鼠的(0.717±0.153),(1.784±0.175)mmol/L(P<0.05),总胆固醇水平也显著高于肥胖抵抗组的(1.711±0.190)mmol/L(P<0.05);肥胖组大鼠的SOCS-3及FAS mRNA表达水平均显著高于对照组和肥胖抵抗组(P<0.05).结论 高脂饮食诱导产生的肥胖和肥胖抵抗大鼠在基因表达调控上可能存在差异,肥胖大鼠的生脂能力增强并可能存在瘦素信号转导通路的抑制.     

肥胖和肥胖抵抗大鼠脂肪组织PPARγ和aP2基因表达的研究

目的观察高脂饮食诱导的肥胖及肥胖抵抗大鼠脂肪组织中PPARγ和aP2 mRNA表达水平的变化。方法31只健康wistar雄性大鼠,随机选取7只作为对照组喂饲基础饲料,24只作为高脂组喂饲高脂饲料。第8周末按体重增量,从高脂组选出5只大鼠作为肥胖组,5只作为肥胖抵抗组,检测各组大鼠血清甘油三酯和总胆固醇,附睾周围脂肪组织中PPARγ和aP2 mRNA表达。结果肥胖组大鼠血清甘油三酯水平显著高于对照组(P<0.05);总胆固醇水平均显著高于对照组和肥胖抵抗组(P<0.05,P<0.01);肥胖组PPARγ和aP2 mRNA表达量高于对照组和肥胖抵抗组(P<0.05)。结论高脂饮食诱导产生的肥胖及肥胖抵抗大鼠在脂肪组织PPARγ和aP2 mRNA表达上存在差异,肥胖大鼠脂肪合成能力增强。     

生长期缺锌对大鼠血清元素的影响

目的:研究膳食锌缺乏对生长期大鼠血清各种元素的影响。方法:刚断奶的SD大鼠,按体重相近配伍,随机分为3组,每组8只:缺锌组(ZD),配饲组(PF),对照组(ZA)。缺锌饲料、正常饲料含锌量分别为0.4mg/kg3、0 mg/kg,实验期15 d。用等离子电感耦合质谱仪(ICP—MS)测定大鼠血清元素锌、铁、铜、硒、锰、钴、铬、钙、镁、镉的含量。结果:缺锌组大鼠:血清铁(573.86±201.71μg/L)明显低于配饲组(1372.37±118.69μg/L)和正常组(1463.55±331.66μg/L)(P<0.05);血清锰(12.28±5.33μg/L)明显高于配饲组(4.24±0.73μg/L)和正常组(3.10±1.10μg/L)(P<0.05);血清铜(534.71±113.77μg/L)、硒(262.55±38.57μg/L)、镁(25.26±9.29μg/dl)低于正常组(铜725.91±158.27μg/L,硒465.90±132.18μg/L,镁39.84±14.10μg/dl)(P<0.05),与配饲组无明显差异(铜566.86±134.96μg/L,硒336.71±112.30μg/L,镁29.59±6.99μg/dl)(P>0.05);相比于配饲组与正常组,血清钙有下降趋势,但差异无显著性(P>0.05),血清钴、铬、镉有升高趋势,但差异无显著性(P>0.05)。结论:膳食锌缺乏影响生长期大鼠体内的微量元素水平。     

慢性三氯化镧暴露对大鼠血糖和血脂水平的影响

目的探讨稀土元素镧的慢性暴露对大鼠血糖、血脂的影响。方法分别以0.1、2和40 mg/kg剂量的三氯化镧(LaC l3)给大鼠经口灌胃,每天1次,90 d后处死;取全血测定糖化血红蛋白(HbA1 c),分离血清测定血糖(G lu)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平。结果与对照组相比,3个LaC l3暴露剂量组大鼠HbA1C、血清G lu、TG和LDL-C水平无统计学意义;0.1和2 mg/kg剂量LaC l3暴露组大鼠血清TC水平分别为(1.38±0.14)mmol/L和(1.37±0.26)mmol/L,与对照组大鼠(1.57±0.14)mmol/L相比有明显降低;0.1 mg/kg剂量LaC l3暴露组大鼠血清HDL-C为(0.79±0.12)mmol/L,比对照组大鼠(0.93±0.10)mmol/L有明显降低。结论0.1~40 mg/kg LaC l3慢性暴露对大鼠血清G lu、TG和LDL-C水平无显著影响;低、中剂量LaC l3慢性暴露可使大鼠血清TC和HDL-C降低。     

不同饲料组成对大鼠血脂的影响

目的比较分析不同种类饲料对大鼠血脂的影响。方法 40只雄性SD大鼠,按体重随机分为4组,每组10只:基础饲料组,5%猪油+1.2%胆固醇组,10%氢化椰子油高糖组和10%牛油高糖组。实验第1周和第6周末检测大鼠血清总胆固醇(total cholesterol, TC)、甘油三酯(triglyceride, TG)、高密度脂蛋白胆固醇(high density lipoprotein-chlesterol, HDL-C)和低密度脂蛋白胆固醇(low density lipoprotein-cholesterol, LDL-C)含量。结果实验第1周,5%猪油+1.2%胆固醇组TC[(2.77±0.47)mmol/L]、LDL-C[(1.53±0.42) mmol/L]显著高于基础饲料组(P<0.05),HDL-C[(1.00±0.14) mmol/L]显著低于基础饲料组(P<0.05),10%氢化椰子油高糖组和10%牛油高糖组TG[(3.58±1.28)和(4.17±1.68)mmol/L]均显著高于基础饲料组(P<0.05)。实验第6周,5%猪油+1.2%胆固醇组和10%氢化椰子油高糖组TC[(2.54±0.60)和(2.73±0.40) mmol/L]均显著高于基础饲料组(P<0.05);10%氢化椰子油高糖组和10%牛油高糖组TG[(2.84±0.79)和(4.16±1.65) mmol/L]均显著高于基础饲料组(P<0.05)。10%氢化椰子油高糖组第6周TC、LDL-C和HDL-C均显著高于第1周(P<0.05),TG显著低于第1周(P<0.05)。结论含有外源性胆固醇的饲料可以使大鼠血清TC和LDL-C显著升高,并降低HDL-C。牛油高糖饲料与氢化椰子油高糖饲料相比,大鼠血清TG水平更高,但能维持TC水平不发生显著改变。提示饲料中添加不同种类的脂肪对大鼠血脂水平的影响也不相同。     

来曲唑法建立PCOS大鼠模型的评价

目的:采用来曲唑灌胃法建立PCOS大鼠模型,并对模型进行全面的评价。方法:选取60只6周龄清洁级SD雌性大鼠,随机分为实验组和对照组各30只,采用来曲唑灌胃诱导PCOS大鼠模型;观察大鼠体重增长、动情周期变化、卵巢形态学和组织学改变;放射免疫法测定大鼠血清性激素水平;电阻抗法测定大鼠血常规;终点法测定大鼠血清生化指标。结果:①动情周期:实验组大鼠灌胃12日后失去了规律的动情周期,实验组大鼠体重增长量明显高于对照组(P<0.05)。②卵巢体积和相对质量:实验组大鼠卵巢的体积和相对质量均明显大于对照组(P<0.05)。③卵巢形态学和组织学:实验组大鼠卵巢表面苍白,体积明显增大,可见大量典型囊状卵泡;组织学检查颗粒细胞层明显减少,白膜增厚,间质增生。④血清激素水平:实验组大鼠黄体生成素(LH)、睾酮(T)浓度明显高于对照组,实验组大鼠卵泡刺激素(FSH)、雌二醇(E2)、孕酮(P)水平明显低于对照组,差异均有统计学意义(P<0.05)。⑤血常规指标:实验组大鼠血红蛋白浓度和红细胞数量均明显大于对照组(P<0.05),白细胞和血小板数量差异无统计学意义(P>0.05)。⑥血清生化指标:实验组大鼠空腹血糖(FPG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)浓度明显大于对照组(P<0.05),两组大鼠血清丙氨酸氨基转氨酶活性(ALT)、天门冬氨酸氨基转氨酶活性(AST)、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)浓度的差异无统计学意义(P>0.05)。结论:来曲唑灌胃法可诱导出较为成功的大鼠模型,PCOS导致大鼠内分泌和代谢指标发生紊乱。     

Lowering serum cholesterol level by feeding a 40% ethanol-eluted fraction from HP-20 resin treated with hot water extract of adzuki beans (Vigna angularis) to rats fed a high-fat cholesterol diet

ObjectiveHot water extract of adzuki beans (Vigna angularis) was subjected to HP-20 resin chromatography. The fraction eluted from the column using 40% ethanol (EtEx.40) was investigated by its effect on serum lipids in rats fed a high-fat cholesterol and/or cholesterol-free high-fat diet.MethodsThe rats were divided into 4 groups. Groups 1 and 2 were fed a high-fat cholesterol diet with or without 3.5% EtEx.40 for 2 wk. Group 3s and 4 were fed a high-fat cholesterol-free diet with or without 3.5% EtEx.40 for 2 wk.ResultsIn the high-fat-cholesterol diet groups, there was no significant difference in food intake in the experimental diet group when compared with the control group. Serum total cholesterol level was significantly decreased in the rats fed the EtEx.40 diet, but there was no difference in fecal excretion of cholesterol and bile acid between the two dietary groups. Conversely, in the high-fat cholesterol-free diet groups, ingestion of EtEx.40 reduced serum triacylglycerol concentration.ConclusionIngestion of EtEx.40 suppressed serum cholesterol level in rats fed the high-fat cholesterol and serum triacylglycerol level in rats fed the high-fat cholesterol-free diet. These mechanisms did not become clear in this experiment.     

几种食物对大鼠血清胆固醇影响的观察

本文通过动物实验,观察了七种食物对大鼠血清胆固醇的影响。其中火麻仁具有阻止实验性血清胆固醇升高的作用,而斑鱼、四菱豆则有促进血清胆固醇升高的作用,实验期中并可看到,雌性大鼠血清胆固醇水平一般有高于雄性的趋势。     

Hypocholesterolemic effects of curcumin via up-regulation of cholesterol 7a-hydroxylase in rats fed a high fat diet

There is an increasing interest in curcumin (Curcuma longa L.) as a cardiovascular disease (CVD) protective agent via decreased blood total cholesterol and low-density lipoprotein-cholesterol (LDL-cholesterol) level. The aim of this study was to investigate further the potential mechanism in the hypocholesterolemic effect of curcumin by measuring cholesterol 7a-hydroxylase (CYP7A1), a rate limiting enzyme in the biosynthesis of bile acid from cholesterol, at the mRNA level. Male Sprague-Dawley rats were fed a 45% high fat diet or same diet supplemented with curcumin (0.1% wt/wt) for 8 weeks. The curcumin diet significantly decreased serum triglyceride (TG) by 27%, total cholesterol (TC) by 33.8%, and LDL-cholesterol by 56%, respectively as compared to control group. The curcumin-supplemented diet also significantly lowered the atherogenic index (AI) by 48% as compared to control group. Hepatic TG level was significantly reduced by 41% in rats fed with curcumin-supplemented diet in comparison with control group (P < 0.05). Conversely, the curcumin diet significantly increased fecal TG and TC. The curcumin diet up-regulated hepatic CYP7A1 mRNA level by 2.16-fold, compared to control group p (P < 0.05). These findings suggested that the increases in the CYP7A1 gene expression may partially account for the hypocholesterolemic effect of curcumin.     

Dietary acetic acid reduces serum cholesterol and triacylglycerols in rats fed a cholesterol-rich diet

To investigate the efficacy of the intake of vinegar for prevention of hyperlipidaemia, we examined the effect of dietary acetic acid, the main component of vinegar, on serum lipid values in rats fed a diet containing 1 % (w/w) cholesterol. Animals were allowed free access to a diet containing no cholesterol, a diet containing 1 % cholesterol without acetic acid, or a diet containing 1 % cholesterol with 0.3 % (w/w) acetic acid for 19 d. Then, they were killed after food deprivation for 7 h. Cholesterol feeding increased serum total cholesterol and triacylglycerol levels. Compared with the cholesterol-fed group, the cholesterol and acetic acid-fed group had significantly lower values for serum total cholesterol and triacylglycerols, liver ATP citrate lyase (ATP-CL) activity, and liver 3-hydroxy-3-methylglutaryl-CoA content as well as liver mRNA levels of sterol regulatory element binding protein-1, ATP-CL and fatty acid synthase (P<0.05). Further, the serum secretin level, liver acyl-CoA oxidase expression, and faecal bile acid content were significantly higher in the cholesterol and acetic acid-fed group than in the cholesterol-fed group (P<0.05). However, acetic acid feeding affected neither the mRNA level nor activity of cholesterol 7alpha-hydroxylase. In conclusion, dietary acetic acid reduced serum total cholesterol and triacylglycerol: first due to the inhibition of lipogenesis in liver; second due to the increment in faecal bile acid excretion in rats fed a diet containing cholesterol.     

抗性淀粉对大鼠肝脏胆固醇代谢相关基因表达的影响

目的:观察抗性淀粉(RS)对大鼠肝脏胆固醇代谢相关基因表达的影响,探讨RS干预胆固醇代谢的作用机制。方法:将18只大鼠随机分为三组,分别饲食正常化学纯合饲料(对照组)、含15%RS纯合饲料(低RS组)和含30%RS纯合饲料(高RS组)6w。采用实时荧光定量PCR技术检测各组大鼠肝组织中胆固醇7a-羟化酶(CYP7A1)、肝清除细胞B1受体(SR-B1)、肝低密度脂蛋白受体(LDLR)mRNA表达水平。检测各组大鼠血胆固醇、盲肠内容物短链脂肪酸(SCFA)含量。结果:大鼠肝组织CYP7A1mRNA、SR-B1mRNA、LDLRmRNA表达水平及盲肠内SCFA含量,高RS组与对照组相比显著增加(P<0.05)。血清胆固醇水平,RS干预组显著低于对照组(P<0.05)。结论:RS通过增强肝组织胆固醇代谢相关基因表达水平,增加盲肠内SCFA含量,发挥降低血胆固醇的作用。     

High-cholesterol diet increases xanthine oxidase and decreases nitric oxide synthase activities in erythrocytes from rats

The aim of this study was to investigate the effects of a high-cholesterol diet on oxidant/antioxidant status and nitric oxide synthase (NOS) activity in erythrocytes from rats. Sixteen Sprague-Dawley–type albino male rats were used in the study. The rats were randomly divided into 2 groups: the control group (group 1) was fed a standard rat diet, and the treated group (group 2) was fed a high-cholesterol diet (4% cholesterol, 1% cholic acid, and 0.5% thiouracil) in addition to standard pellet rat diet for 3 months. At the end of the study period, blood samples were obtained from the rats under ether anesthesia. Oxidant (malondialdehyde level, sensitivity to oxidation value, and xanthine oxidase [XO] activity) and antioxidant parameters (antioxidant potential value, superoxide dismutase, catalase, and glutathione peroxidase activities) were studied in erythrocyte preparations. Activities of erythrocyte NOS and arginase enzymes and serum total cholesterol levels were also measured. We observed that serum total cholesterol levels, erythrocyte XO activities, and sensitivity to oxidation values significantly increased in group 2 (cholesterol fed) compared with the control group (group 1). Erythrocyte NOS activities were also found to decrease in group 2. In conclusion, our results suggest that cholesterol feeding causes an increase in XO activity and a decrease in NOS activity in the erythrocytes from rats. The increase in XO activity may render the erythrocyte membranes sensitive to oxidant stress, and the decrease in NOS activity in the erythrocytes may increase cardiovascular disease risk via reduced endothelial relaxation.     

Iron overload alters glucose homeostasis,causes liver steatosis,and increases serum triacylglycerols in rats

The objective of this study was to investigate the effect of iron overload with a hyperlipidemic diet on the histologic feature of hepatic tissue, the lipid and glycemic serum profiles, and the markers of oxidative damage and stress in a rat model. Twenty-four male Fischer rats, purchased from Experimental Nutrition Laboratory, Federal University of Ouro Preto, were assigned to 4 equal groups, 2 were fed a standard cholesterol-free diet (group C or control and CI or control with iron) containing 8.0% soybean oil and 2 were fed a hyperlipidemic diet (group H or hyperlipidemic and HI or hyperlipidemic with iron) containing 1.0% cholesterol and 25.0% soybean oil. A total of 50 mg of iron was administered to rats in groups CI and HI in 5 equal doses (1 every 3 weeks for a 16-week period) by intraperitoneal injections of 0.1 mL of iron dextran solution (100 g Fe²⁺/L; Sigma, St Louis, Mo). The other rats in groups C and H were treated in a similar manner but with sterile saline (0.1 mL). Irrespective of the diet, iron excess enhanced serum triacylglycerols (P < .05) and reduced serum glucose and glycated hemoglobin levels (P < .05) but did not affect serum cholesterol concentration. Histologic analysis showed steatosis in groups H and to a lesser extent in HI. No significant differences (P > .05) were observed in paraoxonase activities or in serum levels of free or total sulfhydryl radicals, malondialdehyde, or total antioxidants. The findings suggest that iron excess in the rat probably modifies lipid metabolism and, as a consequence, alters glucose homeostasis and increases the level of serum triacylglycerols but not of cholesterol.     

全谷豆复合包经SREBP-2、LDLR和Visfatin调控胆固醇代谢机制的探讨

目的探讨全谷豆复合包改善脂代谢紊乱大鼠胆固醇代谢的可能机制。方法 44只雄性SD大鼠随机分为高脂模型组、米面组、全谷豆复合包组和阴性对照组,分别给予对应饲料连续喂养8周。实验前后测定大鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)含量;实验结束后股动脉取血,分离脏器称重,计算脏体比和脂体比,检测血清中Visfatin浓度、肝脏组织中SREBP-2 mRNA和LDLR mRNA的表达以及脂肪组织中VisfatinmRNA的表达。结果与高脂模型组和米面组相比,全谷豆复合包组大鼠体重、血清TC、TG、LDL-C水平显著下降(P<0.05),血清HDL-C水平显著升高(P<0.05)。全谷豆复合包组大鼠血清Visfatin水平显著低于高脂模型组和米面组(P<0.05);与高脂模型组和米面组比较,全谷豆复合包组大鼠肝脏组织中SREBP-2 mRNA和LDLRmRNA的表达显著升高(P<0.05),脂肪组织中Visfatin mRNA的表达明显降低(P<0.05)。结论全谷豆复合包可以改善高脂膳食诱导的脂代谢紊乱大鼠的血脂和Visfatin水平,可能的机制是全谷豆复合包能增加SREBP-2和LDLR的mRNA表达,降低Visfatin mRNA的表达,经SREBP-2、LDLR和Visfatin途径调控胆固醇的代谢,起到维持TC和LDL-C的正常水平,改善脂代谢紊乱的作用。     

Adzuki resistant starch lowered serum cholesterol and hepatic 3-hydroxy-3-methylglutaryl-CoA mRNA levels and increased hepatic LDL-receptor and cholesterol 7alpha-hydroxylase mRNA levels in rats fed a cholesterol diet

We examined the effects of adzuki bean resistant starch on serum cholesterol and hepatic mRNA in rats fed a cholesterol diet. The mRNA coded for key regulatory proteins of cholesterol metabolism. The control rats were fed 15 % cornstarch (basal diet, BD). The experimental rats were fed BD plus a 0.5 % cholesterol diet (CD), or a 15 % adzuki resistant starch plus 0.5 % cholesterol diet (ACD) for 4 weeks. The serum total cholesterol and VLDL + intermediate density lipoprotein + LDL-cholesterol levels in the ACD group were significantly lower than those in the CD group throughout the feeding period. The total hepatic cholesterol concentrations in the CD and ACD groups were not significantly different. The faecal total bile acid concentration in the ACD group was significantly higher than that in the BD and CD groups. Total SCFA and acetic acid concentrations in the ACD group were significantly higher than those in the CD group but there were no significant differences in the concentrations between the ACD and BD groups. The hepatic LDL-receptor mRNA and cholesterol 7alpha-hydroxylase mRNA levels in the ACD group were significantly higher than those in the CD group and the hepatic 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase mRNA level in the ACD group was significantly lower than in the CD group. The results suggest that adzuki resistant starch has a serum cholesterol-lowering function via enhancement of the hepatic LDL-receptor mRNA and cholesterol 7alpha-hydroxylase mRNA levels and faecal bile acid excretion, and a decrease in the hepatic HMG-CoA reductase mRNA level, when it is added to a cholesterol diet.