静脉瘀血对静脉动脉化皮瓣微循环的影响

目的 研究静脉瘀血对静脉动脉化皮瓣微循环的影响。方法 ３０只新西兰大白兔随机分为Ａ、Ｂ、Ｃ３组，Ａ组为动脉皮瓣，Ｂ组为静脉动脉化皮瓣，Ｃ组为静脉阏血的静脉化皮瓣，每组各１０只。术后３０分钟，１，２，３，４，５小时分别应用显微电视系统通过耳窗对各组皮瓣微循环进行动态观察，７天后记录皮瓣成活情况。结果 静脉瘀血的静脉动脉化皮瓣微循环血流缓慢，微血栓逐渐增多，皮瓣成活率低。结论 静脉瘀血可破坏静脉动脉化     

静脉瘀血对静脉动脉化皮瓣微循环的影响

目的研究静脉瘀血对静脉动脉化皮瓣微循环的影响。方法 30只新西兰大白兔随机分为 A、B、C 3组,A 组为动脉皮瓣,B 组为静脉动脉化皮瓣,C 组为静脉瘀血的静脉动脉化皮瓣,每组各10只。术后30分钟,1,2,3,4,5小时分别应用显微电视系统通过耳窗对各组皮瓣微循环进行动态观察,7天后记录皮瓣成活情况。结果静脉瘀血的静脉动脉化皮瓣微循环血流缓慢,微血栓逐渐增多,皮瓣成活率低。结论静脉瘀血可破坏静脉动脉化皮瓣静脉系统的代偿机制,影响皮瓣血液灌流,从而导致皮瓣成活率下降。     

动脉化静脉皮瓣微循环特点的探讨

动脉化静脉皮瓣是晚近皮瓣移植领域出现的一种新型皮瓣，其有着独特的诱人之处，但由于其成活机制未明，因此有必要深化此项研究。本实验采用显微电视系统直接通过兔耳透明窗对皮瓣微血管进行动态观察，探讨动脉化静脉皮瓣移植早期微循环变化的规律。材料与方法选用新西兰...     

温度对动脉化静脉皮瓣微循环的影响

用红外线烤灯于兔耳透明窗内动脉化静脉皮瓣局部加温，观察加温前及皮温升到２８℃、３２℃、３５℃、４０℃、４５℃时微循环变化情况，可见对皮瓣有明显作用，随着皮温升高起到加速血流、扩张血管及增加血流量的作用，但超过４０℃到达４５℃时则出现凝血倾向、血管开放数减少。经图象分析及统计学处理发现皮温在３２－４０℃范围内均可使皮瓣处于较好的微循环状态，而其中３５℃左右最佳。     

微元生物活性纤维对动脉化静脉皮瓣微循环的影响

目的 研究微元生物活性纤维对动脉化静脉瓣微循环的影响。方法 选用20只新西兰大白兔，随机分为A、B两组，A组为微元生物活性纤维组（n=10)，B组为对照组（n=10)，应用显微电视系统直接观察兔耳透明窗的方法，在放大1000倍条件下研究动脉化静脉皮瓣微循环血流。结果 微元生物活性纤维组微循环血流改善较对照组明显。结论 微元生物活性纤维可改善动脉化静脉皮瓣微循环。     

动脉化静脉皮瓣微循环方式的实验研究

目的　探讨动脉化静脉皮瓣移植早期微循环主要方式。方法　选用 2 0只新西兰大白兔 ,随机分为实验组 (动脉化静脉皮瓣 )和对照组 (动脉皮瓣 ) ,每组各 10只。应用显微电视系统直接观察兔耳透明窗的方法 ,在放大10 0 0倍下研究动脉化静脉皮瓣微循环血流。结果　动脉化静脉皮瓣动脉血逆行灌注后主要经细静脉之间的交通支汇入回流静脉。结论　血流由细静脉→交通支→细静脉。是动脉化静脉皮瓣移植早期微循环的主要方式以维持皮瓣早期成活     

微元生物活性纤维对动脉化静脉皮瓣微循环的影响

目的研究微元生物活性纤维对动脉化静脉皮瓣微循环的影响.方法选用20只新西兰大白兔,随机分为A、B两组,A组为微元生物活性纤维组(n=10),B组为对照组(n=10),应用显微电视系统直接观察兔耳透明窗的方法,在放大1000倍条件下研究动脉化静脉皮瓣微循环血流.结果微元生物活性纤维组微循环血流改善较对照组明显.结论微元生物活性纤维可改善动脉化静脉皮瓣微循环.     

微元生物活性纤维对动脉化静脉皮瓣微循环的影响

目的　研究微元生物活性纤维对动脉化静脉皮瓣微循环的影响。方法　选用 2 0只新西兰大白兔 ,随机分为A、B两组 ,A组为微元生物活性纤维组 (n =10 ) ,B组为对照组 (n =10 ) ,应用显微电视系统直接观察兔耳透明窗的方法 ,在放大 10 0 0倍条件下研究动脉化静脉皮瓣微循环血流。结果　微元生物活性纤维组微循环血流改善较对照组明显。结论　微元生物活性纤维可改善动脉化静脉皮瓣微循环。     

动脉化静脉皮瓣坏死原因的探讨及皮瓣改造

应用微循环显微镜活体观察动脉化静脉皮瓣，发现术后早期皮瓣微血管绝大部分处于关闭状态，原因与静脉短路循环，高压高氧动脉血损伤微血管内上以细胞以及微血栓形成等有关。７２小时后微血管灌流明显改善，根据皮肤扩张器能增组织血管密度，以及静脉内皮细胞对动脉血流需逐渐适应的特点，对皮瓣进行改造后，其成活率明显提高。     

Retrograde arterialized venous flap: an experimental study

An experimental model was established to study circulation in retrograde arterialized venous flaps (RAVF). Venous flaps measuring 7 x 4 cm with a matching venous system were harvested from both forearms of 10 fresh human cadavers. In each trial, both flaps were simultaneously perfused with heparinized human blood driven by a pulsatile circulation model. In each trial there was one flap with retrograde perfusion, and one flap with antegrade perfusion. Clinical assessment, measurement of outflow, and angiographic examination with digitally assisted assessment after 3 h of perfusion showed better results for retrograde perfusion in 8 of the 10 trials. This study indicates that blood circulation in the periphery of arterialized venous flaps can be enhanced by retrograde arterialization.     

动脉化静脉皮瓣两种灌流方式比较的实验研究及临床应用

目的 探讨两种不同灌流方式对游离动脉化静脉皮瓣存活效果的影响。方法 将20只日本大耳白兔40侧后肢建立成吻合股动、静脉浅支的动脉化静脉皮瓣模型。采用自身对照，实验组为逆静脉瓣供血顺静脉瓣回流，对照组为顺静脉瓣供血逆静脉瓣回流。临床上采用逆静脉瓣供血顺静脉瓣回流方式的皮瓣5例。结果 实验组皮瓣存活16侧，部分存活2侧，坏死2侧。对照组皮瓣存活6侧，部分存活11侧，坏死3侧。两组差异有统计学意义（P〈0．05）。临床应用5例，4例完全存活，1例表皮坏死，脱痂后愈合。结论 建立适当的供血与回流比例是动脉化静脉皮瓣存活的关键，保证供血前提下增加静脉回流能提高动脉化静脉皮瓣的存活率。如果不能增加回流，减少超量的供血可提高皮瓣的存活。     

Microcirculation of a venous flap: an experimental study with microspheres in rabbits.

In recent years, it has been found that maintenance of venous circulation alone may support a small flap with no direct arterial inflow. The clinical application of a venous flap has potential in the field of microsurgery. The purpose of this study was to evaluate the haemodynamics within a pedicled venous flap in rabbits, compared with those of a composite graft. Pedicled venous flaps and composite grafts were raised from the abdominal walls of 30 adult New Zealand rabbits. Flap survival was measured and recorded and blood flow studies with microspheres were done for seven days. The viability of the pedicled venous flaps was much better than that of the composite grafts. At two weeks 24 of the venous flaps (80%) showed more than 75% surviving, but 29 (97%) of the composite grafts had less than 25% surviving. The results suggest that the blood flow through a patent vein maintained in a venous skin flap can provide enough nutrients for the flap to survive during the initial three days until neovascularisation. The venous flap receives more blood flow than a composite graft. We conclude that a venous flap depends on blood supply from the axial vein in addition to neovascularisation to maintain its survival.     

Free transplantation of venous network pattern skin flap: an experimental study in rabbits

Free skin flaps using rabbit ear to replace rabbit scalp have been accomplished using only arteriovenous and venous-venous anastomosis, without arterio-arterial anastomosis. The technique produced excellent graft survival in 30 of 33 rabbits. Without the vascular anastomosis control grafts did poorly. The mechanism of flow reversed revascularization is discussed.     

Investigation of metabolism and perfusion in arterialized venous replantation: experimental study in rabbits.

Replantation of amputated digits at distal levels is difficult because the vessels are either too small or absent in the amputated part. Clinically unconventional anastomoses have been tried in such instances. Arterialization of the venous system in the amputated part was also utilized for this purpose. In this study, a digital replantation model utilizing the amputated rabbit ear was used to evaluate the survival, metabolism, and perfusion of unconventionally replanted parts. Mean survival areas were measured and perfusion studies with technetium 99m, blood gases, pH, blood glucose and lactate levels, tissue glucose and lactate levels, and histological evaluations were performed. Surviving areas did not reveal a significant difference. Perfusion of the arterialized venous replants were not as good as the conventionally replanted ears. The blood glucose and lactate levels in the afferent vein were initially near normal arterial levels but almost reached normal venous levels in the samples taken 24 hours after the arterialized venous replantation. Tissue glucose levels were lower than the conventionally perfused tissue, and the lactate levels were higher, but these two metabolite levels were normalized in later samples. The results of the metabolic and perfusion studies are interpreted to elucidate the survival mechanisms in the arterialized venous replants. As a result, arterialized venous replantation may be supported by the favorable results of metabolic and perfusion studies in our experimental model.