VEGF及其受体KDR在宫颈癌的联合表达与局部肿瘤血管生成的关系

目的:探讨血管内皮生长因子及其受体KDR在早期宫颈癌的表达及其对宫颈癌肿瘤血管生成的作用。方法:采用免疫组织化学SP法检测18例宫颈上皮内瘤样病变(cervicalintraepithelialneoplasm,CIN)、75例早期宫颈癌(invasivecarcinomaofcervix,ICC)和15例正常宫颈上皮(normalcervicalepithelium,NCE)中VEGF和KDR的表达情况,并检测其中微血管密度(CD34标记)。结果:在ICC中,VEGF和KDR主要表达于癌细胞膜和(或)细胞浆;而CD34主要表达于癌巢间质血管内皮细胞。从NCE到CIN再到ICC,VEGF与KDR的阳性表达率以及MVD均显著升高(P<0.01)。在ICC中,VEGF、KDR阳性表达者其MVD分别显著高于VEGF、KDR阴性表达者(P<0.05)。VEGF在ICC的表达与KDR显著正相关(r=0.56,P<0.01),并且两者均与MVD显著正相关(前者r=0.60,P<0.01;后者r=0.33,P<0.01)。VEGF与KDR均阳性表达者,其微血管密度显著高于两者均阴性表达者(P<0.01)。结论:VEGF及其受体KDR表达在宫颈癌肿瘤血管生成中起上调作用,两者均过度表达,肿瘤血管生成显著增加。检测VEGF及其受体KDR的联合表达对进一步了解宫颈癌血管生成情况以及寻找抗肿瘤血管生成治疗新靶点有一定价值。     

VEGF及其受体KDF在宫颈癌的联合表达与局部肿瘤血管生成的关系

目的:探讨血管内皮生长因子及其受体KDR在早期宫颈癌的表达及其对宫颈癌肿瘤血管生成的作用.方法:采用免疫组织化学SP法检测18例宫颈上皮内瘤样病变(cervical intraepithelial neoplasm,CIN)、75例早期宫颈癌(invasive carcinoma of cervix,ICC)和15例正常宫颈上皮(normal cervical epithelium,NCE)中VEGF和KDR的表达情况,并检测其中微血管密度(CD34标记).结果:在ICC中,VEGF和KDR主要表达于癌细胞膜和(或)细胞浆;而CD34主要表达于癌巢间质血管内皮细胞.从NCE到CIN再到ICC,VEGF与KDR的阳性表达率以及MVD均显著升高(P<0.01).在ICC中,VEGF、KDR阳性表达者其MVD分别显著高于VEGF、KDR阴性表达者(P＜0.05).VEGF在ICC的表达与KDR显著正相关(r=0.56,P<0.01),并且两者均与MVD显著正相关(前者r=0.60,P<0.01;后者r=0.33,P<0.01).VEGF与KDR均阳性表达者,其微血管密度显著高于两者均阴性表达者(P<0.01).结论:VEGF及其受体KDR表达在宫颈癌肿瘤血管生成中起上调作用,两者均过度表达,肿瘤血管生成显著增加.检测VEGF及其受体KDR的联合表达对进一步了解宫颈癌血管生成情况以及寻找抗肿瘤血管生成治疗新靶点有一定价值.     

SABC-DS法检测肺癌中VEGF-KDR及其对肿瘤血管形成的影响

目的 肿瘤血管生成是肿瘤细胞生长、进展和转移的基本步骤。探讨肺癌肿瘤血管形成中的VEGF-KDR途径，为肺癌的治疗提供理论依据。方法 实验组为106例肺癌石蜡包埋标本，对照组为5例正常肺组织。应用SABC-DS双标免疫组织化学染色方法，检测两种与肿瘤血管形成和间质状态有关的调控因子即VEGF(血管内皮生长因子)和Flk-1／KDR(VEGF受体-2)的蛋白表达，采用图像分析技术检测MVD(微血管密度)，分析它们与临床病理学参数的差异和关系。采用SPSS(Ver8．0)统计软件包进行数据处理。结果 肺癌中MVD值与VEGF蛋白表达有显著性差异。MVD分级与VEGF蛋白表达间存在正相关。MVD值与Flk-I／KDR蛋白表达有显著性差异。MVD分级与Flk-I／KDR蛋白表达间存在正相关。结论 VEGF和受体Flk-I／KDR的结合促进微血管形成，应用SABC-DS法检测VEGF和Flk-I／KDR可预测肿瘤血管新生和肺癌血道转移。     

VEGF及其受体KDR在胃癌组织新生血管中的作用

目的探讨血管内皮生长因子VEGF及其受体KDR在胃癌新生血管形成中的作用.方法应用免疫组织化学技术,检测60例胃癌组织VEGF及KDR蛋白表达和以CD105标记的微血管密度,分析VEGF、KDR和MVD及其与肿瘤大小、肿瘤分化、浸润深度、淋巴结转移、组织学分级和预后关系.结果VEGF及KDR阳性者MVD值显著高于阴性者(t=3.85,t=3.76,P＜0.01).VEGF、KDR表达和MVD与胃癌浸润深度(x2=8.31,P＜0.01;x2=5.05,P＜0.05;t=2.24,P＜0.05)、淋巴结转移(x2=10.62,P＜0.01;x2=5.66,P＜0.05;t=5.19,P＜0.05)、组织学分级(x2=11.05,P＜0.01;x2=8.21,P＜0.01;t=2.11,P＜0.05)密切相关.VEGF及KDR表达阳性或高MVD的胃癌患者5年生存率较低(x2=5.18,P＜0.05;x2=8.94,P＜0.01;t=3.51,P＜0.01).结论VEGF及KDR与胃癌新生血管生成密切相关,对胃癌的生长和浸润转移有促进作用,VEGF及KDR和以CD105标记的MVD可作为反映胃癌生物学行为的客观指标.     

再谈肺癌中血管生成与其预后的关系

目的:研究肺癌中血管内皮生长因子(vascularendothelialgrowthfactorVEGF)、激酶插入结构域受体(ki鄄naseinsertdomainingreceptorKDR)表达水平和微血管密度(Micro-vasculardensityMVD)及其与肺癌预后的关系。方法:采用免疫组化方法(LSAB法),检测114例肺癌组织中的VEGF、KDR表达水平及MVD。结果:肺癌组织中VEGF、KDR高表达组患者5年生存率分别为18.18%和21.19%,显著低于低表达组61.19%和52.74%(P<0.01);MVD高密度组患者5年生存率为17.27%,显著低于低密度组52.74%(P<0.01)。VEGF、KDR表达水平及MVD与生存期均呈显著负相关(P<0.01),其相关强度依MVD、VEGF、KDR顺序逐渐降低。多因素分析表明,pTNM分期仍是预测肺癌预后最有价值的指标,但VEGF、KDR表达水平,MVD也是判断肺癌预后较有价值的指标。VEGF、KDR表达水平越高,MVD越大,其无瘤生存期和5年生存率越短。结论:肺癌中VEGF、KDR基因的表达异常及MVD的增大在肺癌的发生、发展、转移和预后中可能起重要作用,检测肺癌中VEGF、KDR基因表达水平及MVD有助于预测肺癌预后。     

淋巴管生长因子对食管鳞癌血管生成、肿瘤转移及预后的影响

目的：探讨淋巴管生长因子与食管鳞癌临床病理参数、血管生成及预后的关系。方法：采用Elivision二步免疫组化法检测51例食管鳞癌手术切除标本中VEGF—C的表达，应用CD34标记肿瘤内微血管，计数微血管密度，对VEGF—C与临床病理参数、肿瘤内MVD以及预后间的关系进行回顾性分析。结果：食管鳞癌淋巴结转移组VEGF—C阳性率和MVD明显增高；VEGF—C阳性组和高MVD组患者的中位生存时间以及3年、5年生存率均明显低于阴性组。VEGF—C与MVD呈正相关。结论：淋巴管生长因子VEGF—C可促进肿瘤微血管生成。VEGF—C阳性、MVD增高可作为食管鳞癌淋巴转移增加以及预后差的指标。     

VEGF及其受体Flt-1、KDR在鼻咽癌组织中的表达及意义

背景与目的:VEGF及其受体Flt-1、KDR在肿瘤血管生成及肿瘤进展中发挥关键作用。本研究探讨鼻咽癌组织中VEGF、Flt-1和KDR表达与鼻咽癌患者临床特征及预后的关系。方法:用免疫组织化学的方法检测127例鼻咽癌标本中VEGF、Flt-1和KDR的表达情况,用SPSS10.0软件分析这三种蛋白表达的相关性、与临床特征的关系以及对患者总生存的影响。结果:VEGF、Flt-1和KDR分别表达于66.9%(85/127)、90.6%(115/127)和88.2%(112/127)的鼻咽癌组织。鼻咽癌组织中VEGF表达与Flt-1和KDR表达呈正相关(r=0.292,P<0.01;r=0.287,P<0.01),Flt-1表达与KDR表达呈正相关(r=0.723,P<0.01)。VEGF蛋白的表达与原发肿瘤的侵犯范围、淋巴结转移、临床分期有关(P=0.04,P<0.01,P=0.02),KDR蛋白表达也与原发肿瘤的侵犯范围有关(P=0.03);三种蛋白表达均与肿瘤局部复发和/或远处转移(P<0.01,P<0.01,P<0.01)、总生存不佳有关(P<0.01,P<0.01,P<0.03)。Cox多因素相关分析显示,年龄>50岁和VEGF阳性为不良预后因素(P<0.01,P<0.01)。结论:VEGF及其受体Flt-1、KDR在鼻咽癌中广泛表达,与鼻咽癌患者的临床特征和预后关系密切。     

血管内皮生长因子及其受体与上皮性卵巢癌发展

目的　探讨血管内皮生长因子 (VEGF)及其受体 (KDR)与上皮性卵巢癌发展的关系。方法　用免疫组化方法检测 4 5例人上皮性卵巢癌中VEGF及KDR的表达 ,分析VEGF及KDR表达与卵巢癌FIGO分期、淋巴结转移及预后的关系。结果　 4 5例上皮性卵巢癌中 ,VEGF、KDR表达阳性率分别为 73.3% (33/ 4 5 )、5 7.8% (2 6 / 4 5 )。Ⅲ～Ⅳ期卵巢癌VEGF表达阳性率 (94 .4 % ,17/ 18)高于Ⅰ～Ⅱ期卵巢癌 (5 9.3% ,16 / 2 7)。淋巴结转移阳性组中VEGF表达阳性率 (10 0 % ,12 / 12 )高于淋巴结转移阴性组 (16 / 2 6 ,6 1.5 % ) ,(P <0 .0 5 )。VEGF表达阴性者生存率高于VEGF表达阳性者 (P =0 .0 4 7)。不同组织学类型上皮性卵巢癌中VEGF表达无明显差异。KDR表达与卵巢癌分期、淋巴转移及预后无关。结论　VEGF与上皮性卵巢癌发展有关。     

肺癌血管内皮生长因子(VEGF)及其受体KDR、Flt1的表达与患者预后的关系

背景与目的 血管内皮生长因子（VEGF）及其受体与肺癌血管生成关系密切，但与肺癌患者预后的关系尚不明确。本研究的目的是对VEGF及其受体KDR、Flt1的表达与肺癌患者预后的关系进行探讨。方法 应用免疫组织化学PV-9000法检测75例具有完整随访资料的肺癌标本中VEGF及其受体KDR、Flt1的表达。结果 VEGF、KDR、Flt1在肺癌组织中的表达较为广泛，主要位于肿瘤细胞、血管内皮细胞、纤维母细胞胞质中，且其表达呈异质性，肺癌组织周边部及坏死区周围的肺癌细胞中表达较强，三者在肿瘤细胞中的阳性率均高于间质纤维母细胞（P〈0．01，P〈0．02，P〈0．02）。肺癌细胞及纤维母细胞VEGF、KDR和Flt1的阳性表达率在术后不同生存时间的三组间差异均具统计学意义（P〈0．01，P〈0．01，P〈0．01；P〈0．01，P〈0．01，P〈0．05）；肺癌细胞VEGF、KDR、Flt1阳性组患者的生存时间均显著低于各相应指标阴性者（P〈0．0001，P〈0．0005，P〈0．0005）。肺癌细胞中VEGF与Flt1受体的表达呈正相关（P〈0．01），纤维母细胞中VEGF与肺癌细胞中Flt1受体的表达呈正相关（P〈0．01），纤维母细胞中VEGF与KDR、Flt1表达分别呈正相关（P〈0．01，P〈0．01）。结论 VEGF可能是促进肺癌细胞生长的重要因子，主要以自分泌途径并附以旁分泌途径通过Flt1发挥作用；VEGF及受体KDR、Flt1对肺癌患者的预后有重要的影响作用。     

壶腹部癌组织血管内皮生长因子的表达及意义

目的探讨壶腹部癌组织中VEGF表达和微血管密度(MVD)水平以及与患者预后的关系.方法22例行手术切除的壶腹部癌石蜡标本,采用免疫组化(SP)方法进行VEGF表达检测和MVD测定.结果22例壶腹癌中,VEGF表达阳性率为50%,淋巴结转移者的VEGF表达阳性率(85.7%,6/7)明显高于无淋巴结转移者(33.3%,5/15)(P＜0.05).MVD均值为26.4±12.8,淋巴结转移者MVD均值(33.1±10.8)明显高于无淋巴结转移者(22.8±11.8)(P＜0.05),TNM分期Ⅲ、Ⅳ期患者的MVD均值(36.3±8.4)明显高于Ⅰ期(18.4±10.1)和Ⅱ期(23.8±13.4)的患者(P＜0.05).VEGF表达阳性者的MVD均值(35.0±9.6)明显高于VEGF表达阴性者的MVD均值(17.7±9.3)(P＜0.01).VEGF表达阳性患者的三年生存率为(9.1%,1/11),明显低于VEGF表达阴性患者(45.5%,5/11)(P<0.05).高血管组患者的三年生存率为(10%,1/10),明显低于低血管组患者(50%,6/12)(P＜0.05).结论VEGF在壶腹部癌组织血管生成中发挥重要作用,VEGF、MVD可能有效地用于评价肿瘤生物学行为、估计患者预后.     

Relationship between Co-expression of Vascular Endothelial Growth Factor and its Receptor, Kinase Insert Domain Containing Receptor and Tumor Angiogenesis in Invasive Carcinoma of the Cervix

Objective The aim of the present study was to determine the expression of vascular endothelial growth factor (VEGF) and its receptor, kinase insert domain containing receptor (KDR), and their significance in regulating tumor angiogenesis in the early stages of cervical cancer. Methods Using the immunohistochemical SP method, the expression of VEGF and KDR was determined in the cancer cells. In addition, the microvessel density (MVD), labeled by CD34 in the tumor stroma, was examined in 18 cases of cervical intraepithelial neoplasms (CIN), 75 cases of early invasive cervix carcinomas (ICC) and 15 specimens of normal cervical epithelium (NCE). Results In ICC cases, VEGF and KDR were mainly expressed in the cellular membrane and/or cytoplasm of tumor cells, while expression of CD34 was found mainly in the vascular epithelial cells of the tumor stroma. The positive expression rate of VEGF and KDR, and the MVD increased remarkably from NCE through CIN to ICC (P< 0.01). For the ICC group, in the patients with positive expression of VEGF and KDR, the MVD was significantly higher than those with negative expression of VEGF and KDR (P<0.05). Expression of VEGF in ICC was positively related to KDR expression (r=0.56,P<0.01 ). The MVD was also positively related to both the expression of VEGF (r=0.60,P<0.01), and KDR (r=0.33,P<0.01). In the cases with both positive expression of VEGF and KDR, the MVD was significantly higher than those in which there was negative expression of both(P<0.01). Conclusion Expression of VEGF and its receptor KDR plays a key role in up-regulating tumor angiogenesis in cervical carcinoma. Co-overexpression of VEGF and KDR results in rapid tumor vasculogenesis. Detection of co-expression of VEGF and KDR may be of value in further understanding tumor angiogenesis and in searching for new targets for anti-angiogenesis therapy in invasive carcinoma of the cervix. This work was supported by the Department of Education of Fujian Province (No. 01B017).     

Ki-67、VEGF在胃肠间质瘤中表达及与MVD的相关性

目的:探讨Ki-67和VEGF在胃肠间质瘤中的表达及与临床病理因素的关系,VEGF、微血管密度(MVD)和细胞增殖之间的相关性。方法:采用免疫组化S-P法检测44例胃肠间质瘤组织中Ki-67、VEGF表达及计数MVD值和Ki-67PI。结果:VEGF、Ki-67在GIST组织中阳性表达分别为77.3%、63.6%,VEGF、Ki-67表达在不同大小的肿块之间有统计学差异(P<0.01);MVD值和Ki-67PI在VEGF阳性和阴性组的比较有统计学差异(P<0.01);VEGF、MVD和Ki-67PI之间呈显著正相关(相关系数分别为0.26、0.44和0.84,P<0.01)。结论:VEGF促进GIST组织中的新生血管形成,为肿瘤组织提供了丰富的血液和营养,并使细胞增殖活性增强,促进肿瘤细胞的增殖、肿瘤的生长、发展和转移;Ki-67PI为GIST的预后判断提供了比较客观的依据。     

VEGF及其受体KDR在垂体腺瘤中的表达与肿瘤血管形成的关系

 目的　探讨血管内皮生长因子(VEGF) 及其受体( KDR) 的表达与垂体腺瘤血管生成的关系。方法　应用免疫组织化学S2P 法检测58 例人垂体腺瘤中的V E GF 及KD R 的表达,并对血管进行染色、计数。结果　其中54 例有VEGF 的表达(占93. 1 %) ,阳性表达主要位于肿瘤细胞胞膜及胞浆; KD R 在47 例中有阳性表达(占81. 0 %) ,阳性表达位于肿瘤血管内皮细胞、肿瘤细胞胞膜及胞浆。VEGF 和KD R 表达与垂体腺瘤的侵袭性密切相关;V E GF 和KD R 高表达组微血管密度明显高于低表达组( P <0. 01) 。结论　VEGF 以旁分泌、自分泌形式协同KDR 促进垂体腺瘤血管的生成,并与垂体腺瘤的侵袭密切相关。     

Microvessel density, VEGF expression, and tumor-associated macrophages in breast tumors: correlations with prognostic parameters

Angiogenesis plays an important role in tumor growth, metastasis, and prognosis. Vascular endothelial growth factor (VEGF) is a potent endothelial mitogen and acts on the angiogenic stimulation of human neoplasias. In infiltrative ductal carcinoma (IDC), VEGF expression is correlated with high vascularity. Tumor-associated macrophages (TAMs) contribute to tumor proliferation, progression and angiogenesis and have a complex role in tumor biology. In this study, the correlations between microvessel density (MVD), VEGF expression, and TAMs and their relations to clinicopathological parameters such as tumor size, metastatic lymph node, mitotic activity index (MAI) and tumor grade were investigated in 48 cases of IDC and 30 infiltrative lobular carcinoma (ILC) cases. MVD showed a significant positive correlation with TAMs, VEGF, metastatic lymph nodes, tumor size and grade in IDC (P < 0.001). In ILC, MVD and tumor size were positively correlated (P = 0.003), while MVD was not correlated with VEGF, TAMs, MAI, metastatic lymph nodes, and grade. These findings are suggestive of angiogenesis stimulation in IDCs by VEGF, driving the macrophages into the tumor area. MVD and TAMs were found to be important prognostic factors in IDCs. On the other hand, however, VEGF did not contribute to angiogenesis in ILCs, and MVD and TAMs did not have any prognostic significance. These results suggest the involvement of factors not related to VEGF in the angiogenesis of lobular carcinoma.     

VEGF及其受体KDR在非小细胞肺癌中的表达及与肿瘤血管形成关系

目的:探讨血管内皮生长因子(VEGF)及其受体(KDR)的表达与非小细胞肺癌(NSCLC)血管形成的关系。方法:分别应用免疫组织化学S-P法和原位分子杂交法检测62例NSCLC组织和16例肺的良性瘤样病变组织中的VEGF和KDRmRNA的表达,并对血管进行染色、计数。结果:62例肺癌组织中46例有VEGF的表达(占74·29%),阳性表达位于肿瘤细胞胞膜及胞质;KDR mRNA在49例中有阳性表达(占79·03%),阳性表达位于肿瘤血管内皮细胞、肿瘤细胞胞膜及胞质。VEGF和KDR mRNA的表达与有无淋巴结转移和临床病理分期密切相关;VEGF和KDR mRNA阳性表达组微血管密度明显高于阴性表达组,P<0·01。结论:VEGF和KDR的表达与NSCLC的发生、发展和转移可能密切相关,可作为评估NSCLC患者预后的一项指标。     

基质金属蛋白酶7和基质金属蛋白酶抑制剂3在骨巨细胞瘤中的表达

目的探讨基质金属蛋白酶7(MMP-7)及基质金属蛋白酶抑制剂3(TIMP-3)在骨巨细胞瘤中的表达及临床意义。方法 38例骨巨细胞瘤标本,通过免疫组化染色方法检测骨巨细胞瘤标本中的MMP-7、TIMP-3和CD34的表达,观察其在非复发肿瘤组和复发肿瘤组中的变化。分组统计学采用t检验、χ2检验和spearman等级相关分析。结果在骨巨细胞瘤中,MMP-7和TIMP-3阳性率分别为97.0%和95.0%。MMP-7的表达和MVD在复发组和非复发组之间的差异有统计学意义(P<0.05),MMP-7与微血管密度(MVD)存在正相关性(P<0.05),但TIMP-3的表达在复发组和非复发组之间的差异无统计学意义(P>0.05),MMP-7和组织学分级(spearman等级相关分析,P>0.05)不存在相关意义,TIMP-3、MVD和组织学分级(spearman等级相关分析,P<0.05)存在相关意义。结论 MMP-7和TIMP-3在骨巨细胞瘤中高度表达,MMP-7可能会加速肿瘤血管形成、细胞增殖并影响骨巨细胞瘤的复发。     

Vascular endothelial growth factor: a therapeutic target for tumors of the Ewing's sarcoma family.

PURPOSE: We have reported previously that intratumoral microvessel density (MVD) is a significant prognostic indicator of event-free survival in the Ewing's sarcoma family of tumors (ESFT). Here, the angiogenic growth factor expression profile and its relationship with MVD has been investigated in ESFT. EXPERIMENTAL DESIGN AND RESULTS: Using ESFT model systems, the potential of these factors as therapeutic targets has been evaluated. A significant correlation (P = 0.02) was observed between vascular endothelial growth factor (VEGF) expression and MVD, consistent with the hypothesis that VEGF regulates the development of microvessels in ESFT. There was no correlation between MVD and any of the other growth factors studied. All six ESFT cell lines studied produced and secreted VEGF; five of six cell lines also secreted placental growth factor, one cell line (A673) at high levels. Tumor conditioned medium induced proliferation of human umbilical vein endothelial cells. Expression of VEGF receptors Flt-1 and Flk-1/KDR was heterogeneous across the cell lines. Both receptor tyrosine kinase inhibitors SU6668 (targets Flk-1/KDR, platelet-derived growth factor receptor-beta, and fibroblast growth factor receptor 1) and SU5416 (targets Flk-1/KDR) as well as anti-VEGF agents rhuMAb-VEGF (bevacizumab) and VEGF Trap delayed s.c. growth of ESFT in mice compared with untreated groups: SU6668 (100 mg/kg/d), SU5416 (25 mg/kg/d), rhuMAb-VEGF (10 mg/kg twice weekly), and VEGF Trap (2.5 or 25 mg/kg twice weekly). CONCLUSIONS: These data suggest that VEGF is the single most important regulator of angiogenesis in ESFT and may be exploited for therapeutic advantage.     

骨髓来源内皮前体细胞促进肿瘤新生血管生成

Objective:Neovascularization of tumor is a complex process.In this study,we aimed to reveal whether the bone marrow-originated endothelial progenitor calls (EPCs) contributed to neovasculature in tumor and the angiogenesis-associated factors,VEGF and B-FGF,enhanced this process.Methods:We had established a mouse model,which were deprived of bone marrow by radiation and transplanted with bone marrow of syngenetic GFP (Green Fluorescence Protein)-transgened mice,then implanted Lewis calls.Immunohistochemical and immunoflourensenca proved the EPCs location in tumors by indentifying colocalization of GFP expression in cells staining with endothelial progenitor cell markers,CD 133,ICAM-1,CD31.The growth statue and MVD of tumor was observed after injection of VEGF or B-FGF.ICAM-1 and VE-cadherin in tumor were detected by Western blot.Results:By immunohistochemical and immunoflourensence,we proved part of bone marrow precursors located in area of tumor angiogenesis and VEGF or B-FGF increased the MVD of tumor.In Western blot,it was found and VEGF or B-FGF up-regulate the expression of ICAM-1,VE-Cadherin.Conclusion:Bone marrow-derived endothelial progenitor cell seem to be recruited in neovasculature induced by tumor.VEGF and B-FGF are key regulators of this process.     

卵巢交界性肿瘤血管内皮生长因子表达和微血管密度的研究

背景与目的:血管生成是实体肿瘤生长、侵袭、扩散转移的关键,微血管密度(microvesselofdensity,MVD)可反映肿瘤的血管形成情况。血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)是具有重要意义的促血管生长因子,它与肿瘤的生长密切相关。本研究检测卵巢交界性肿瘤组织中VEGF的表达和MVD,探讨它们与临床病理特征及预后的关系。方法:采用免疫组化SP法和原位杂交技术检测69例卵巢交界性肿瘤、18例卵巢良性肿瘤和27例卵巢恶性肿瘤组织中VEGF蛋白及VEGFmRNA的表达,用FⅧ因子单克隆抗体标记新生血管内皮,计数并计算MVD。结果:VEGF蛋白、VEGFmRNA在卵巢交界性肿瘤组织中的表达均介于卵巢良性肿瘤与卵巢恶性肿瘤之间,其结果差异均有显著性(P<0.05);也与卵巢交界性肿瘤的临床分期、MVD值密切相关(P<0.05);但VEGF与卵巢交界性肿瘤的组织分型、有无腹腔种植无关(P>0.05)。结论:VEGF与卵巢肿瘤的生长、侵袭、转移密切相关;检测VEGF有助于判断卵巢肿瘤的恶性程度,为判断卵巢交界性肿瘤患者的预后提供依据。