匍枝千里光的化学成分

目的:;对千里光属中匍枝千里光的化学成分进行研究。方法:;使用常压硅胶柱层析法对匍枝千里光乙醇提取物的二氯甲烷萃取部分的化学成分进行分离和纯化, 通过理化方法和NMR 对分离得到的化合物进行结构鉴定。结果:; 从匍枝千里光Senecio filiferus中分离并鉴定了8个已知化合物:5个单萜化合物:(4S, 6R)6羟基胡椒酮(Ⅰ),(1S,2S)5异丙基2甲基环已3烯1,2醇(Ⅱ), (4R, 6S)6羟基胡椒酮(Ⅲ), (4R,5S)4羟基5异丙基2甲基环已2烯酮(Ⅳ),(4R,5R)4羟基5异丙基2甲基环已2烯酮(Ⅴ),其它类:(6S,7E)6hydroxy4,7megastigmadien 3,9dione (Ⅵ), 4, 4二甲基 1, 7庚二酸(Ⅶ),正十五烷酸(Ⅷ)。结论:;其中Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ单萜化合物为千里光属中首次分离得到,其它Ⅵ,Ⅶ,Ⅷ化合物为首次从匍枝千里光中分离得到。      

欧洲千里光化学成分的研究

目的 研究欧洲千里光Senecio vulgaris的化学成分。方法 采用硅胶柱色谱进行分离纯化,通过理化性质和波谱数据鉴定结构。结果 从欧洲千里光甲醇提取物中分离鉴定了11个化合物,分别为环阿尔廷-23Z-烯-3β,25-二醇(cycloart-23Z-ene-3β,25-diol,1)、1β,6α-二羟基桉烷-4(15)-烯(1β,6α-dihydoxyeudesm-4(15)-ene,2)、黑麦草内酯(loliolide,3)、1β,5α-二当归酰氧基-桉烷-(15)-烯(1β,5α-diangeloyloxy-eudesm-(15)-ene,4)、1β,7α-二羟基桉烷-4(15)-烯(1β,7α-dihydroxyeudesm-4(15)-ene,5)、刺参酮(oplopanone,6)、1-羟基-4-氧代-2,5-环己二烯-1-醋酸甲酯(蓝花楹酮,jacaranone,7)、1′-羟基-2′-甲氧基-4′-环己烯-醋酸甲酯(1′-hydroxy-2′-methoxy-4′-oxocyclohexanacetate methyl,8)、4-(醋酸甲酯)-4-羟基-环己酮(4-carbomethoxymethyl-4-hydroxycyclohexanone,9)、1-羟基-2,6-二甲氧基-4-环己烯-醋酸甲酯(1-hydroxy-2,6-dimethoxy-4-oxocyclohexanacetate methyl,10)、2-［2,2-二甲基-6-氧-7-二氢-1,3-苯并二氧戊环-3(6-氢)-基］-醋酸甲酯(2-［2,2-dimethyl-6-oxo-7-dihydro-1,3-benzodioxol-3(6H)-yl］ aceate methyl,11)。结论 11个化合物均是首次从欧洲千里光中分离得到。     

披针新月蕨细胞毒活性成分研究

目的 研究民族药披针新月蕨Abacopter is penangiana化学成分并评价其细胞毒活性。方法 以硅胶柱、凝胶柱色谱分离,制备HPLC纯化,采用MS,1、2 D-NMR等波谱方法进行结构鉴定,MTT法测试各化合物的细胞毒活性。结果 从披针新月蕨中分离鉴定了6个化合物,分别为4(S),5,7-三羟基-4′-甲氧基-6,8-二甲基-2(R)-黄烷-5,7-O-β-D-二葡萄糖苷［4(S),5,7-trihydroxy-4′-methoxy-6,8-dimethyl-2(R)-flavan-5,7-O-β-D-diglucopyranoside, eruberin B, 1］; 5,7-二羟基-4(S),4′-二甲氧基-6,8-二甲基-2(R)-黄烷-5,7-O-β-D-二葡萄糖苷［5,7-dihydroxy-4(S),4′-dimethoxy-6,8-dimethyl-2 (R)-flavan-5,7-O-β-D-diglucopyranoside,eruberin C, 2］;5,7-二羟基-4′-甲氧基-6-羟甲基-8-甲基-2″,4(S)-氧-2(R)-黄烷-5-O-β-D-葡萄糖苷［5,7-dihydroxy-4′-methoxy-6-hydroxymethyl-8-methyl-2″,4(S)-oxido-2(R)-flavan-5-O-β-D-glucopyranoside,3］;5,7-二羟基-4′-甲氧基-6,8-二甲基-2″,4(S)-氧-2(R)-黄烷-5-β-D-葡萄糖苷［5,7-dihydroxy-4′-methoxy-6,8-dimethyl-2″,4(S)-oxido-2(R)-flavan-5-O-β-D-glucopy ranoside,eruberin A,4］;高圣草素［5,7,4′-trihydroxy-3′-methoxyflavanone, homoeriodictyol,5］;对-甲氧基桂皮醛(p-methoxycinnamaldehyde,6)。化合物3、4对L929及HeLa细胞的IC50值分别约为13.05、24.75 μg/mL,25.11、18.32 μg/mL。结论 化合物3为新的天然产物,化合物2、5、6首次从新月蕨属植物中分得,化合物3、4对L929和HeLa具有显著抑制活性。     

腺梗議莶的化学成分研究(Ⅲ)△

本文报道从腺梗議莶地上部分分到5个化合物,根据IR、1H,13CNMR和MS等光谱测定鉴定为二香草基四氢呋喃(epoxylignan,Ⅰ),槲皮素(quercetin,Ⅱ),丁香醛(syringic aldenyde,Ⅲ)2-氨基-3(3′-羟基-2′-甲氧苯基)-1-丙醇[2-amino-3(3′-hydroxy-2′-methoxyl phenyl)-1-propanol,Ⅳ],D-甘露醇(D-mannitol,Ⅴ),其中Ⅰ,Ⅲ,Ⅳ和Ⅴ系首次从该植物地上部分得到.     

麻叶千里光抗菌化学成分的研究(Ⅱ)

目的研究麻叶千里光S enecio cannabif olius抗菌活性成分。方法以革兰氏阳性菌金黄色葡萄球菌S taphy lococcus aureus、枯草芽孢杆菌B acillus subtilis和阴性菌大肠杆菌E scherich ia coli为受试菌,采用体外抑菌圈法追踪抗菌活性部分,利用各种色谱技术进行分离纯化,根据理化性质和谱学数据进行结构鉴定。结果从麻叶千里光中分离得到5个化合物,分别鉴定为5-羟基吡啶-2-甲酸甲酯(E-13)、6-羟基-7,7a-二氢-2-(6H)-苯骈呋喃酮(E-14)、2-(1,4-二羟基环己烷基)-乙酸(E-16)、3-羟基环己酸(E-17)、4-羟基苯甲醛(B-14)。结论它们均为从千里光属植物中首次分到。     

梓实中的新苯并呋喃

目的研究梓实的化学成分。方法采用各种色谱技术进行分离纯化,通过理化常数和光谱分析鉴定化合物的结构。结果通过UV、IR、ESI-MS、1H-NMR、13C-NMR、1H-1HCOSY、HMQC、HMBC、NOESY和CD等光谱分析鉴定新化合物为2(S)-[3′-羟基-5′-甲氧基]苯基-3(S)-甲酸乙酯基-6-反丙烯酸乙酯基-8-甲氧基苯并呋喃[2(S)-(3′-hydroxy-5′-methoxy)-benz-3(S)-ethoxycarbonyl-6-trans-ethylacrylate-8-methoxy-benzofuran]。结论该化合物为新化合物,命名为梓呋新。     

苏门白酒草的化学成分研究

目的 研究苏门白酒草的化学成分。方法 运用溶剂萃取、硅胶和聚酰胺柱色谱、重结晶等方法分离纯化,并通过核磁共振谱鉴定化合物的结构。结果 从苏门白酒草中分离鉴定了12个化合物：芹菜素-7-O-β-D-葡萄糖醛酸苷-6″-甲酯(Ⅰ)、芹菜素-7-O-β-D-葡萄糖醛酸苷(Ⅱ)、金圣草黄素-7-O-β-D-葡萄糖醛酸苷-6″-甲酯(Ⅲ)、金圣草黄素-7-O-β-D-葡萄糖苷(Ⅳ)、4′-羟基黄芩素(Ⅴ)、金合欢素-7- O-芸香糖苷(Ⅵ)、金圣草黄素(Ⅶ)、菠甾醇-3-O-β-D-葡萄糖苷(Ⅷ)、菠甾醇-3-O-β-D-葡萄糖苷-6′-O-棕榈酸酯(Ⅸ)、( 2S,3S,4R,8E)-8,9-二脱氢植物鞘氨醇(2′R )-2′-羟基二十二、二十三、二十四、二十五烷酰胺(Ⅹ)、天师酸(Ⅺ)、菠甾醇(ⅩⅡ)。结论 化合物Ⅰ～Ⅺ为首次从该植物中分离得到,化合物Ⅰ～Ⅶ和Ⅸ～Ⅺ为首次从白酒草属植物中分离得到。     

蝴蝶戏珠花茎的化学成分

从蝴蝶戏珠花(Viburnum plicatum Thunb.var.tomentosum Miq.)茎中分离得到10个化合物,分别鉴定为:3,4,5-三甲氧基苯酚-1-O-β-D-葡萄糖苷(1),isotachioside(2),tachioside(3),koaburaside(4),丁香酸葡萄糖苷(5),羽扇豆醇(6),熊果酸(7),绿原酸(8),5-O-咖啡酰基-奎宁酸丁酯(9),苜蓿素-7-O-β-D-葡萄糖苷(10)。化合物1-10均为首次从蝴蝶戏珠花中分离得到。     

大血藤的化学成分研究(Ⅱ)

目的 研究大血藤Sargentodoxa cuneata的化学成分。方法 应用多种色谱技术对大血藤进行分离纯化,并通过NMR等波谱方法鉴定化合物的结构。结果 从大血藤乙醇提取物的正丁醇和醋酸乙酯部位分离得到13个化合物,分别鉴定为毛柳苷(1)、(-)表儿茶素(2)、3,4-二羟基苯乙醇(3)、原儿茶酸(4)、N-(对羟基苯乙基)阿魏酸酰胺(5)、3,4-二羟基苯乙醇葡萄糖苷(6)、野菰苷(7)、4-羟苯基-乙基-6-O-(E)咖啡酰基-β-D-葡萄糖苷(8)、绿原酸乙酯(9)、lyoniresin-4′-yl-β-glucopyranoside(10)、2(3′,4′-二羟苯基)1,3-胡椒环-5-醛(11)、丁香酸葡萄糖苷(12)、8,8′-bis-(dihydroconiferyl)diferuloylate(13)。结论 化合物7～13均为首次从该植物中分离得到。     

金钗石斛中的酚性成分

目的对金钗石斛茎的化学成分进行研究。方法运用多种色谱学技术对金钗石斛的化学成分进行分离,并根据光谱数据鉴定化合物的结构。结果从该植物中分离得到12个酚性化合物,其结构分别为二氢松柏醇二氢对羟基桂皮酸酯(Ⅰ),香草醛(Ⅱ),罗布麻宁(Ⅲ),对羟基苯甲醛(Ⅳ),丁香醛(Ⅴ),丁香酸(Ⅵ),丁香乙酮(Ⅶ),α-羟基丁香丙酮(Ⅷ),松柏醛(Ⅸ),二氢松柏醇(Ⅹ),2-羟基苯丙醇(Ⅺ)和3-羟基-4-甲氧基苯乙醇(Ⅻ)。结论上述化合物均为首次报道从金钗石斛中分离得到,化合物Ⅰ,Ⅲ~Ⅻ为首次报道从石斛属植物中分离得到。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.