The therapy with the large dosage of methylprednisolone for the Bell palsy

Background：ThemainpathologicalimpairmentsincludeedemaoffacialnerveanddifferentleveldegenerationofmyelinsheathoraxiscylinderinBell＇spalsy．Theprognosisofthedis－easeresultsfromseveredegreeofthediseaseandwhethertreat－mentsaretimelyornot．ThetherapywithlargedosagesofMethylprednisolone（MPS）inveinforBell＇spalsy，canrelievelocaledema，improvenerveconduction，avoidprogressiveimpair－mentsofmyelinesheathandaxiscylinderandpromoterepairsofmyelinesheath．Objective：Todiscusstheeffectsoflargedosa…     

重组人红细胞生成素在肿瘤相关贫血中的应用

Itiswellknowthatrecombinanthumanerythropoietin（rhEPO）caneffectivelymanagechronicrenalfailure－relatedanemia．rhEPOcorrectedsymptoms，improvedqualityoflifeofanemiapatientsandreducedrisksassociatedwithheterotransfusion．Intheendof１９８０＇sandbeginningof１９９０＇s，someresearchersstudiedtherapeuticeffectofrhEPOinthetreatmentofanemiaduetootherdiseasesespeciallycancer－relatedanemiasuchaschemotherapyorradiotherapyinducedanemiaandneoplasticanemia．Manyfactorssuchasbleeding，infec－tion，hemo…     

帕罗西汀对卒中后抑郁病人的功能恢复作用

Depressionisthecommoncomplicationofstroke，whichoccursinatleast３０％ofthestrokepatients．Depressionaffectsthepatients＇rehabilitationsignificantly．Itiscommonlyadmittedthat５－HTreup－takeinhibitorshavegoodeffectontreatingpoststrokedepression（PSD）．Thispaperstudiestheclinicaltherapeuticeffectofparoxetineoneofthe５－HTreuptakeinhibitorsintreatingPSDanditseffectonthepatients＇nervefunctions．Comparedwiththetricircularantide－pressionreagents，suchasdoxepin，themechanismofthetherapeuticeff…     

The in fluence of acute cerebral infarction early treatment on the patients'''' recovery of nerve function

Background：８０％strokepatientsstillleavebehindfunctionaldisturbanceindifferentdegreeaftertreatment，whichmakegreatinfluenceonpatients＇livinglevel．Prognosisofacutecerebralin－farction（ACI）isdifferentwhenwetreatitindifferentwayintheearliertime．Objective：ToobservetheBarthelindexanddisabilitydegreeofdischargepatientswhoreceivedoneofthreeearlytreatmentmethods，todiscusstheinfluencesmadebyACIearlytreatmentmethodsonpatients＇prognosis，livingqualityanddisabilityde－gree．Design：Weselecte…     

多梗死性痴呆患者脑脊液肿瘤坏死因子和神经元特异性烯醇化酶的测定及临床意义

InAsia，theincidencerateofvasculardementiaishigherthanthatofAlzheimer＇sdisease．Vasculardementiaisoneofthemostcommondiseasesofseniledementia．Multi－infarctdementia（MID）isthemostcommontypeinvasculardementia．Inthestudywereporthere，wemeasuredtheconcentrationsofTNF－αandNSEincere－brospinalfluid（CSF）of７２patientswithcerebralinfarction（CI）and５５patientswithMID，soastoevaluatetheroleofthechangesofTNF－αandNSEinthepathogenesisofMID．１Subjectandmethod１．１SubjectThesubjects…     

Comprehension rehabilitation trea tment on 60 cases of posthemiplegic shoul-der-hand syndrome

BACKGROUND：Shoulder－handsyndromeleadstolimitofmotorfunctionwhichwillcausepermanentdeformityoffingers，impairfunctionofhandifitisn＇ttreatedtimely．Atpresent，therearen＇teffectivemethodtoshoulder－handsyndrome．OBJECTIVE：Toobservetheeffectofcomprehensiverehabil－itationtreatmentonshoulder－handsyndrome．UNIT：DepartmentofNeurology，SecondHospitalofDandongCitySUBJECTS：６０patientsservedassubjectsinthisstudyinclud－ing３１malesand２９femalesaged４０－８１（mean：５７）yearsoldw…     

Early systemic rehabilitation nursing and effects evaluation after hand burn

INCIDENCEANDCAUSESOFHANDDEFORMITYAFTERBURNUglyappearanceanddysfunctionisverycommonafterburn．In１２７７casesofburntreatedinXijingHospital，theincidenceofdeformityis６４．１％．Lukaihuaanalyzed３１００casesofhandburnandthoughtthatdeformityandsecondaryplasticswasbecause：（１）Skingraftwasn＇tperformedindegreeIIburn；（２）PeripheraldeepdegreeIIwoundsurfacewasleftafterearlyremovalofscar；（３）Lessautogenousskinsourceandstampskingraftwasperformed；（４）Patientsweren＇tin－st…     

Effect of early radiotherapy combined with operation on keliod

INTRODUCTIONInpresentdays，treatmentofkeliodincludessimpleoperation，radi－ation，drugtherapy，immunetherapy，lasertherapyandfreezingtherapy，buttheireffectsaren＇tsatisfying．Operationcombinedwithradiationonkeliodhasachievedsatisfyingeffect．MATERIALSANDMETHODSMaterials３５burnpatientswithkeliodtreatedindepartmentofoncology，sec－ondhospitalofXi＇anJiaotonguniversitywereselectedincluding２１malesand１４femalesaged１７－４３（mean：２９．３）yearsold．Thelargestareaofkeliodwas９cm…     

pSVPoMCAT微基因修饰雪旺氏细胞移植治疗实验性脊髓损伤的酶及免疫组化评价

Genetherapyofspinalcordinjury（SCI）remainsintheexper－imentalstage．Now，neurotrophicfactortomodifySchwanncell（SC）orfibroblastsareimplantedintoSCIregiontoobservetheregenera－tionsofaxons犤１犦．WefirstbuildPo－５＇－flankinginducedmicrogeneforbasicproteinofmyelinsheathwhichistransientlynamedaspSVPoMcat．ThepSVPoMcatgenewasintroducedintoSCthroughcationliposome．ItwasprovedthatpSVPoMcatcouldenhancefunc－tionofSCandelongateitssurvivalperiodinvitro犤２犦．pSVPoMcatwasimplantedintoS…     

Impact of early rehabilitation therapy on post stroke depression

INTRODUCTIONPoststrokedepression（PSD），thecommonlyobservedcomplicationofcerebrovasculardiseases，seriouslyaffectspatients＇function，re－coveryandlife，leadingtoselfinjuryorsuicide．Earlyrehabilitationtherapyisbeneficialforfunctionalrecovery，reductionofdisabilityrate，improvementofqualityoflifeandreliefofpain，thusreducingincidenceofdepression．MATERIALANDMETHODSMaterialAllsubjectswereinpatientsofourhospitalduringthreeyears．Inclu－sioncriteriawasthefollowing：（１）diagnosisc…     

Quick search of differentially expressed genes of EB virus resulting immortal human B cells by suppression subtractive hybridization

AIM:To clone and identify genes differentially expressed in the EB virus-transformed human B cell.METHODS:Suppression subtractive hybridization was used to construct the library which contains the differentiately expressed cDNAs in EB virus-transformed human B cell .Then the isolated genes were cloned and sequenced and identifed by RT-PCR ,Nucleic acid homology searches were performed using the BLAST program.RESULTS:By this technique,4 differentiately expressed gene cDNA fragments of EB virus-transformed human B cell were obtained .CONCLUSION:SSH is an effective method to isolate differentiately expressed genes.     

Lymphoma in cotton-top marmosets after inoculation with Epstein-Barr virus: tumor incidence, histologic spectrum antibody responses, demonstration of viral DNA, and characterization of viruses

6 of 20 cotton-top tamarins (Saguinus oedipus) inoculated with Epstein-Barr virus (EBV) developed diffuse malignant lymphoma resembling reticulum cell or immunoblastic sarcoma of man. Hyperplastic lymphoreticular lesions were induced in three additional animals; in two instances the hyperplastic lesions regressed. Inapparent infection with development of antibody occured in eight animals. In two animals there was no evidence of EBV infection. One animal died in the first week after inoculation of parasitic infection. 10 animals uninoculated or mock-inoculated developed neither lymphoproliferative disease nor antibody. The malignant lymphoma appeared to arise from a cell with an uncleaved vesicular nucleus found in the center of the germinal follicle. The prominent cytologic features of this cell were extensive formation or rough endoplasmic reticulum and elaboration of the cytoplasmic membrane with microvilli. Cell lines derived from these tumors did not have receptors for complement. IgFc, or sheep erythrocytes, and the cell lines adhered to glass and plastic. EB nuclear antigen was found in imprints of two lymph nodes, one with lymphoma and one with hyperplasia. EB virus DNA was detected directly in the tumors of three animals and in cell lines from two lymphomas. Typical herpes virus particles were found in supernatant fluids from cell lines obtained from lymph nodes with tumors and hyperplasia, as well as in lines derived from blood leukocytes of marmosets with inapparent infection. These virus preparations had the biologic property characteristic of EBV, namely, stimulation of cellular DNA synthesis and immortalization of human lymphocytes. The virus derived from two cell lines was neutralized by reference human sera with EBV antibody and not by antibody-negative human sera. The virus derived from the experimental lesions is thus indistinghishable from human EBV. The marmoset has enhanced susceptibility to oncogenesis by EB virus. Among identified factors which may play a role in the heightened tumorigenicity of EB virus in this species are the increased production of virus by transformed cells and the absence of membrane receptors for complement or IgFc on transformed cells.     

Lymphocytes transformed by Epstein-Barr virus. Induction of nuclear antigen reactive with antibody in rheumatoid arthritis

Sera from approximately two-thirds of patients with rheumatoid arthritis contain an antibody which is reactive with a nuclear antigen present in human B-lymphocyte tissue culture cells. The immunological reaction can be demonstrated by precipitation and immunofluorescence. Evidence is present that the reactive nuclear antigen is associated with Epstein-Barr (EB) virus-transformed lymphocytes. Normal human peripheral blood lymphocytes did not contain the nuclear antigen reactive with rheumatoid arthritis sera, but after infection with EB virus, they showed increasing amounts of reactive nuclear antigen as the cells were transformed into continuous lines. Several established human and simian lymphocyte cell lines known to carry EB viral genomes were shown to contain rheumatoid arthritis-associated nuclear antigen. Evidence is presented which suggests that the rheumatoid arthritis-associated nuclear antigen is different from the previously described EB nuclear antigen.     

The molecular biology of Epstein-Barr virus

Epstein-Barr virus (EBV) was discovered in continuously growing tumor cells derived from African patients with Burkitt's lymphoma. In the intervening twenty years, much biological and biochemical information has been accumulated. The virus infects B lymphocytes and occupies a unique position among human herpesviruses in that it is the only one which is capable of forming a latent infection whereby complete copies of the virus genome persist in growth transformed cells. Since there are no fully permissive cell systems of virus replications, only the established B cell lines are available for study of the molecular events of EB virus in infected cells. A viral cycle consists of four stages, latent, early replicative, middle replicative and late replicative stages. In the latent state, only small parts of the viral genome are transcribed and express transformation proteins: nuclear antigens (EBNAs) and lymphocyte determined membrane antigen (LYDMA). After reactivation of viral genome and during a productive cycle, more than 50 RNAs are expressed and over 30 viral-specified polypeptides are detectable by immunoprecipitation with a high titer human anti-EBV serum. During the early replicative stage, early antigens (EA) and DNA enzymes, both necessary for DNA synthesis, are synthesized. In the late replicative stage, about 30-40 mRNA are transcribed and two major late antigen complexes, viral capsid antigens (VCA) and membrane antigens (MA), are identified. These antigens are indispensable for the formation of virions.     

Epstein-Barr virus transformation of human pre-B cells

In vitro infection of human B lymphocytes with Epstein-Barr virus (EBV) results in establishment of B lymphoblastoid cell lines that reflect normal B cell phenotypes. In this study we have investigated whether immature B cells from fetal bone marrow and liver can serve as targets for EBV. The fetal bone marrow cells were readily transformed by EBV. Among the resulting cell lines, five were surface Ig (sIg)-negative. Three B cell-associated antigens defined by monoclonal antibodies were expressed to the same extent on the fetal cell lines, whether they belonged to the sIg- or sIg+ group. The various differentiation stages that these cell lines may represent are discussed.     

Transforming activity and antigenicity of an Epstein-Barr-like virus from lymphoblastoid cell lines of baboons with lymphoid disease.

Two lymphoblastoid cell lines were established from baboons with lymphoid disease. Cells of these lines were positive for complement and Fc receptors but lacked sheep cell receptors, theraby indicating B-cell origin. The cells contained antigens which cross-reacted with Epstein-Barr virus (EBV), viral capsid antigen (VCA), early antigen (EA) and membrane antigen (MA). Both lines released virus with in vitro transforming activity for lymphocytes of several primate species including humans. Cells of the original lines and transformed cells showed no staining for EB nuclear antigen (EBNA). The virus was neutralized by anti-MA positive baboon and human sera. Baboon virus and EBV had different but overlapping in vitro host-cell ranges.     

Frequencies of specific antibody-producing cells for sheep red blood cells and for phosphorylcholine in human tonsillar B lymphocytes transformed by Epstein-Barr virus

The frequencies of transformed B cells and of transformed specific antibody-producing cells in human tonsillar lymphocytes infected with Epstein-Barr virus (EBV) were determined by limiting dilution analyses. The latter was corrected by the former and the frequencies of specific antibody-producing cells in EBV-transformed B cell populations were obtained. The frequencies of specific B cells for sheep red blood cells were calculated to be one in 500 to 2,000 EBV-transformed tonsillar B cells obtained from four donors, while those of specific B cells for a hapten, phosphorylcholine, were one in 700 to 5,000. The method may be useful to define the repertoire of specificities in the human B cell populations.     

具有阵发性睡眠性血红蛋白尿症表型的B淋巴细胞株的建立

建立阵发性睡眠性血红蛋白尿症和正常人的Ｂ细胞株,用以研究ＰＮＨ。方法用ＥＢ病毒感染ＰＮＨ患者和正常人的Ｂ淋巴细胞,感染后的Ｂ淋巴细胞用流式细胞仪式分析其细胞表型,然后用免疫磁珠分选ＣＤ＾－５５、ＣＤ＾－５９Ｂ淋巴细胞,并用极限稀释法对分选后的细胞进行克隆,克隆后的细胞株用聚合酶链反应技术扩增免疫球蛋白重链可变区基因重排带,验证其克隆性。结果建立了２株分别来自于２例ＰＮＨ患者的ＣＤ＾－５５、ＣＤ＾－     

Helper and suppressor T-cells regulating killer cells of EB virus infected cells

Using an in-vitro human lymphocyte system, a study was performed to determine whether helper T-cells and suppressor T-cells control the generation of killer cells against autologous B-cells transformed by Epstein-Barr (EB) virus. When T-cells were treated with mitomycin C and reacted in the presence of macrophages with target cells bearing HLA-DR, helper T-cells were induced but suppressor T-cells were not. In contrast, when T-cells were reacted in the absence of macrophages to target cells lacking HLA-DR, suppressor T-cells but not helper T-cells were induced. The culture supernatant of the T-cells which showed suppressor activity also suppressed the generation of killer cells. The thus-induced suppressor T-cells also suppressed the HLA incompatible allogeneic killer cells which were directed against autologous B-cells transformed by EB virus and also autologous killer cells directed against allogeneic target cells. These observations indicated that there is no HLA restriction between the suppressor T-cells and the killer cells and the suppression is antigen nonspecific.     

类胚体诱导脐带间充质干细胞向生殖细胞的分化

背景:脐带间充质干细胞能否在体外和体内微环境条件下衍生为卵母细胞,对女性生殖的维持及卵母细胞的再生均具有十分重要的意义。目的:进一步验证体外微环境类胚体对脐带间充质干细胞向生殖细胞分化的影响。方法:将脐带间充质干细胞进行悬滴培养,使其形成类胚体,进而采用将类胚体与人或小鼠卵巢颗粒细胞共培养、卵泡液条件培养基培养等方法,体外诱导脐带间充质干细胞向早期生殖细胞分化。结果与结论:①将脐带间充质干细胞进行悬滴培养,其能够形成类胚体。②流式细胞仪检测结果:类胚体形成5d后,其中SSEA-1阳性细胞占15.61%。③免疫组织化学检测结果:形成5d的类胚体与人或小鼠的卵巢颗粒细胞共培养,10d后生殖系标记物SCP3、生长分化因子9阳性表达,而颗粒细胞及采用卵泡液培养的类胚体均无表达。结果表明,脐带间充质干细胞体外悬滴培养可形成类胚体,与人或小鼠卵巢颗粒细胞共培养后均表达生殖系特异性标记物。