共查询到20条相似文献,搜索用时 31 毫秒
1.
A. V. Bocharov T. G. Vishnyakova I. N. Baranova V. S. Repin M. A. Kornyushin L. N. Kask G. T. Sukhikh Yu. A. Blidchenko M. V. Eremeeva 《Bulletin of experimental biology and medicine》1998,126(4):1052-1055
Two types of binding sites for high-density lipoproteins (Kd=1 μg/ml and 20 μg/ml) were identified on human fetal hepatocytes and designated as P1 and P2. Ligand blotting has shown that P1 site is protein with Mr 100 kD and P2 site involves two proteins with Mr 105 and 110 kD. P2 proteins were not detected when incubation with ligands was shortened from 24 h to 30 min. Molecular weight and activity
of P1 did not change considerably after reduction of disulfide bonds.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 10, pp. 459–463, October, 1998 相似文献
2.
E. S. Dremina T. V. Vakhrusheva V. S. Sharov O. A. Azizova Yu. A. Vladimirov 《Bulletin of experimental biology and medicine》1995,119(2):135-139
It is demonstrated that the content of the primary products of lipid peroxidation reaches the maximum after about 1-h incubation
with Cu2+ and then declines. At a Cu2+ concentration of about 10–15 μM, the content of lipid peroxidation products is maximal; it does not rise with a further increase
in the Cu2+ concentration. Comparison of the kinetics of lipid peroxidation in different lipoprotein fractions shows that low density
lipoproteins are much more strongly oxidized than high density lipoproteins. A strong positive correlation between the amplitude
of the chemiluminescence burst and the diene conjugate content is established in 79 independent measurements.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
o
2, pp. 144–148, February, 1995 相似文献
3.
Wei Huang T. G. Vishnyakova A. V. Bocharov E. V. Zaitseva E. G. Frolova D. D. Sviridov V. S. Repin J. -L. Nano P. Rompal 《Bulletin of experimental biology and medicine》1994,117(1):52-55
It is shown that glucocorticoids play a key regulatory role directed toward the maintenance of an optimal level of binding
and internalization of HDL3 in hepatocytes. Their stimulatory effect on the expression of HDL receptors proves to be independent of changes in the CH
content in parenchymal cells.
Translated fromByulleten' Eksperimentalnoi Biologii i Meditsiny, Vol. 117, N
o
1, pp. 50–53, January, 1994 相似文献
4.
V. V. Belousova A. M. Dudchenko L. D. Luk'yanova 《Bulletin of experimental biology and medicine》1995,119(1):23-27
It is demonstrated that the lactate-Po2 dependence is the same in hepatocytes of rats with high and low resistance to hypoxia and does not correlate with phasic
changes in the ATP concentration in the 890–50 μM O2 region. Strong activation of lactate formation against the background of ATP decrease indicates that glycolysis is not the
major mechanism determining the steady-state ATP level in the cell and affecting the ATP-Po2 relationship in a wide range of oxygen concentrations. The intensity of glycolysis in hepatocytes of rats with high resistance
to hypoxia is markedly increased after periodic adaptation to hypoxia but remains practically unchanged in the hepatocytes
of low-resistance rats. This indicates that fundamentally different compensatory mechanisms are involved in this process in
the liver of high- and low-resistance rats.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
o
1, pp. 28–32, January, 1995 相似文献
5.
Yu. V. Bukanova E. I. Solntseva V. G. Skrebitskii 《Bulletin of experimental biology and medicine》1998,126(4):1006-1009
A high-threshold (−20 mV) K+ current was recorded from isolated edible snail neurons by a two-microelectrode voltage clamp technique. This current consisted
of three components: fast-inactivating K+ currents (IA), noninactivating K+ current (IKD), and Ca2+-dependent K+ current (IK(Ca)). Different cells had one to three components of K+ current. Vinpocetine increased IA, moderately inhibited IKD (by 30–50%) and strongly suppressed IK(Ca) (by 60–90%). Inhibition of IK(Ca) was not related to the effect of vinpocetine on the inward Ca2+ current. When K+ current consisted of all three components, the effect of vinpocetine on the ionic current amplitude was opposite at different
potentials.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 10, pp. 408–411, October, 1998 相似文献
6.
I. S. Proskuryakova 《Bulletin of experimental biology and medicine》1995,119(6):633-636
It is demonstrated that cytoplasmic extract of rat pup liver stimulates cellular and intracellular regeneration of hepatocytes
exposed to the toxic agent CCl4. Injection of liver cytosol stabilizes lysosomal membranes, increases the coefficient of energy efficiency of mitochondria,
and activates proliferation of polyploid hepatocytes.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
o
6, pp. 656–659, June, 1995
Presented by D. S. Sarkisov, Member of the Russian Academy of Medical Sciences 相似文献
7.
A. A. Boldyrev E. R. Bulygina G. G. Kramarenko 《Bulletin of experimental biology and medicine》1996,121(3):253-256
The susceptibility of Na,K-ATPase from bovine brain to various compounds containing active oxygen radicals is assessed. Sodium
nitroprusside slightly inhibits Na,K-ATPase, while light-induced NOo radicals (controlled by the rate of ascorbate oxidation) have no effect on the enzyme. When added in concentrations equally
effective in the ascorbate oxidation assay, hydrogen peroxide and sodium hypochlorite inhibit Na,K-ATPase by 70 and 25–30%,
respectively. The Fe-dinitrosyl-cysteine complex is the most potent (K0.5=20 μM) inhibitor of Na,K-ATPase. It is demonstrated that different free oxygen radicals accumulated in the ischemic brain
cause different kinds of damage to Na,K-ATPase.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N
o
3, pp. 275–278, March, 1996
Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences 相似文献
8.
I. M. Rodionov A. N. Kosyakov O. S. Tarasova E. N. Timin 《Bulletin of experimental biology and medicine》1995,120(5):1083-1086
Arterial pressure lability and its variations were examined in unrestrained rats following selective elimination of adrenergic
or purinergic sympathetic influences on the circulatory system. Both the α1-andrenoceptor blocker prazosin and the nonselective α-adrenoceptor blocker phentolamine lowered the arterial pressure without
affecting its lability. When P2x purine receptors were desensitized with α,β-methyleneATP, the resulting pronounced hypotension was accompanied by a two-fold
increase in the lability of mean arterial pressure.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, Nno 11, pp. 461–464, November, 1995
Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences 相似文献
9.
V. V. Alabovskii E. J. Cragow A. A. Vinokurov 《Bulletin of experimental biology and medicine》1999,127(4):363-365
Subtotal 30-min ischemia leads to myoglobin release and increases water content in the heart. Reperfusion partially restores
the developed pressure. Addition of furosemide (a Na+, K+, 2Cl−-sumport blocker) or NMA (inhibitor of Na+/H+-exchange) to perfusate decreases myocardial water content, reduces myoglobin loss, and completely restores myocardial contractile
function. The low-rate perfusion of isolated heart and its reperfusion with solutions containing DIOA (inhibitor of K+, Cl−-co-transport) or IAA-94 (Cl− channel blocker) increases water accumulation and myoglobin release from the myocardium, and deteriorated its contractile
function during reperfusion.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 127, No. 4, pp. 400–403, April, 1999 相似文献
10.
M. V. Bilenko V. G. Ladygina I. A. Tarakanova 《Bulletin of experimental biology and medicine》1996,121(1):67-71
The toxic effects of H2O2, paraquat, and oxidized low density lipoproteins are more expressed on superoxide dismutase-deficientE. coli strains than on its wild-type strains, and the effect of tert-butyl-hydroperoxide is less dependent on the presence or absence
of this enzyme in the bacterium, whereas that of bleomycin does not depend on it at all. The toxicity of the agents increases
in the following series: H2O2<oxidized low density lipoproteins<tert-butyl-hydroperoxide <paraquat≪bleomycin. A culture ofE. coli strains AB 1157 and JI 132 may be used for assessing the toxic effect of prooxidants, and anE. coli JI 132 culture with oxidative stress induced by prooxidants as a test system for detecting the potential antioxidants and
assessing the mechanism of the action.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N
o
, pp. 74–79, January, 1996
Presented by A. I. Archakov, Member of the Russian Academy of Medical Sciences 相似文献
11.
N. V. Nosova V. L. Kozhura I. S. Novoderzhkina 《Bulletin of experimental biology and medicine》1998,125(3):251-254
An increase in activity of Ca2+, Mg2+-dependent endonucleases on the second hour of hypotension coincided with the presence of DNA fragments in agarose gel. A
correlation was established between the duration of hypotension, Ca2+, Mg2+-dependent endonuclease activity, and intensity of nuclear DNA fragmention. Apopotosis of hepatocytes is triggered during
ischemia and develops during reperfusion.
Translated fromByulleten' Eksperimental'noi Biologii i Meditisiny, Vol. 125, No. 3, pp. 285–288, March, 1998 相似文献
12.
It is shown that the serotonin receptors 5-HT1c, 5-HT3, and 5-HT4 and α2-adrenergic receptors are involved in the regulation of audiogenic seizures in DBA/2 mice, and that the effects of these serotonin
receptors on the duration and magnitude of convulsive activity are the opoosite of those produced by α2-adrenergic receptors.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
o
4, pp. 381–383, April, 1995 相似文献
13.
I. N. Kusmartseva S. G. Afanas'ev N. V. Cherdyntseva I. V. Kondakova S. A. Kusmartsev 《Bulletin of experimental biology and medicine》1998,125(2):190-193
It is shown that nonadhesive bone marrow cells from patients with stomach cancer suppress phytohemagglutinin-stimulated proliferation
of peripheral blood lymphocytes of healthy donors and proliferation of Molt-4-human lymphoma cellsin vitro. Suppressive activity of bone marrow cells from cancer patients is not mediated through prostaglandin secretion, since indomethacin
has no effect on it. Addition of neutralizing monoclonal antibodies to transforming growth factor-21, 22, and 23 partially reduces this suppressive effect. Suppressive effect of bone marrow cells from patients with stomach cancer is partially
mediated through production of nitric oxide, since the inhibitor of its synthesis N9-monomethyl-L-arginine diminishes the inhibiting effect of bone marrow cells from cancer patients on phytohemagglutinin-stimulated
proliferation of peripheral blood T cells from healthy donors.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 125, No. 2, pp. 217–220, February, 1998 相似文献
14.
S. B. Tkachenko V. N. Kokryakov I. P. Ashmarin S. V. Grachev A. A. Kubatiev 《Bulletin of experimental biology and medicine》1994,118(6):1291-1294
The effect of the total fraction of human defensins (HNP-1, HNP-2, and HNP-3) on the cytoplasmic Ca2+ content ([Ca2+]i) in the platelets of healthy donors was studied. At concentrations of 0.1–40 μg/ml and an incubation time of 10 min defensins
have no effect on [Ca2+]i in platelets labeled with Fura-2AM. However, at higher concentrations (100 μg/ml) they increased platelet [Ca2+]i. In addition, defensins (40 μg/ml) inhibited the Ca2+ increase in platelets induced by thrombin, adenosine diphosphate, and the lipopolysaccharide ofS. typhimurium endotoxin. The most pronounced inhibitory effect was observed in a suspension of thrombin-stimulated platelets. It is shown
that the effect of human defensins on the functional activity of platelets is due to the alterations in the intracellular
Ca2+.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
o
12, pp. 600–603, December, 1994 相似文献
15.
N. K. Zenkov M. I. Dushkin E. B. Men'shikova Yu. I. Ragino E. N. Pivovarova 《Bulletin of experimental biology and medicine》1996,122(4):998-1000
The antioxidant activity of the lipophilic hormone melatonin, “an ideal inhibitor of free radicals,” is studied in models
of cellular (peritoneal mouse macrophages) and copper-induced oxidation of low density lipoproteins. Oxidative modification
of low density lipoproteins is assessed by accumulation of thiobarbituric acid reactive substances and degradation of125I-labeled lipoproteins in a fresh culture of macrophages. Melatonin inhibits in a dose-dependent manner cellular and copper-induced
oxidation of lipoproteins and production of the superoxide anion radical by macrophages, the mean concentrations of 50% inhibition
being 300, 1230, and 900 μM, respectively.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 122, No. 10, pp. 399–402, October, 1996 相似文献
16.
V. A. Nagornev L. S. Kositskaya V. S. Rabinovich P. G. Nazarov A. V. Polevshchikov L. E. Vasil'eva 《Bulletin of experimental biology and medicine》1998,125(4):408-412
Synthesis of acute phase transmitters, migration and degranulation of pseudoeosinophils in hepatocytes, and damage to hepatocytes
are observed during, the first few weeks of atherogenic diet. It is hypothesized that the acute phase reaction determines
disturbances in the synthesis and catabolism of lipoproteins in the liver.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 125, No. 4, pp. 460–464, April, 1998 相似文献
17.
R. A. Krasnoperov 《Bulletin of experimental biology and medicine》1998,125(4):420-422
Significant correlation was established in 52-week-old rats between serum calcitonin content and density of sublemmal granules
at the vascular pole of C-cells (N
Vsg)∶r
xy=0.922,p<0.001. Statistical simulation methods made it possible to determine the confidence interval of biological availability of
endogenous calcitonin: 87–100% (p≥0.95).
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 125, No. 4, pp. 474–476, April, 1998 相似文献
18.
V. V. Tertov V. V. Kaplun I. A. Sobenin A. N. Orekhov 《Bulletin of experimental biology and medicine》1996,121(1):38-39
Sialylated and desialylated low-density lipoproteins from human blood are shown to differ markedly in physicochemical parameters.
The latter lipoproteins have a smaller particle size, are denser and more electronegative, and tend to aggregate more readily
than the former.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N
o
1, pp. 42–43, January, 1996
Presented by E. I. Chazov, Member of the Russian Academy of Sciences 相似文献
19.
V. I. Kulinskii L. A. Usov G. Z. Sufianova A. A. Sufianov 《Bulletin of experimental biology and medicine》1994,117(6):626-628
Adenosine and its analogs N6-cyclohexyladenosine (CHA) and N-ethylcarboxamideadenosine (NECA) are highly effective when injected into the lateral ventricle
in very low doses (by 1–2 orders lower than subcutaneously). The protective effect of A-agonists is considered to be mediated
by a central mechanism and realized via A-receptors of the brain: the effects of low doses of A-agonists are mediated by A1-receptors, whereas higher doses exert an extra A2-effect.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N
o
6, pp. 622–624, June, 1994
Presented by P. V. Sergeev, Member of the Russian Academy of Medical Sciences 相似文献
20.
The binding of 2G3 mouse antitheophylline monoclonal antibodies (affinity constant Kaff=2×1010 liter/mol) to theophylline is studied using isothermal titration microcalorimetry. Thermodynamic parameters of the binding
are: enthalpy change ΔH=−0.23 kcal/mol; Gibbs free energy change ΔG=−16.42 kcal/mol; entropy change ΔS=0.054 kcal/(mol×K).
Regression analysis shows a two-site kinetic binding model. A great contribution of entropy component to the free energy change
of 2G3 antibody binding to theophylline is indicative of an entropy-dependent process. The entropy-dependent nature of the
binding presumably determines the binding kinetics.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 11, pp. 570–573, November, 1997 相似文献