PCR检测沙眼衣原体及其在男性尿道感染中的意义

应用多聚酶链反应(PCR)检测604例临床已确诊的男性泌尿系感染患者衣原体(CT)感染情况,发现阳性率为:急性淋菌性尿道炎26.9％(117/435)、淋菌后尿道炎48.5％(32/66)、非淋菌性尿道炎31.1％(32/103)。认为PCR技术是临床快速诊断男性衣原体尿道炎的有效方法。     

PCR检测衣原体及支原体感染的临床意义

应用聚合酶链反应（ＰＣＲ）对１２８例疑为淋菌性或非淋菌性尿道炎患者进行沙眼衣原体和解脲支原体检测，结果获得阳性者５３例，衣原体阳性者３３例，占６２．３％，支原体阳性者２８例，占５２．８％，其中衣原体和支原体均阳性者８例，占１５．１％。并就ＰＣＲ检测的诊断价值及临床意义进行了讨论。认为积极开展ＰＣＲ技术对衣原体和支原体进行检测，是提高非淋菌性尿道炎诊断率及治愈率的重要措施。     

PCR检测非细菌性前列腺炎沙眼衣原体的初步研究

对３０例非细菌性前列腺炎（ＮＢＰ）患者的前列腺按摩液（ＥＰＳ）沙眼衣原体（ＣＴ）进行了聚合酶链反应（ＰＣＲ）检测，并与经二乙氨基葡聚糖处理的ＨｅＬａ细菌培养法进行对比研究，结果发现，６例ＰＣＲ和细胞培养双阳性，２１例ＰＣＲ和细胞培养双阴性，３例两种检测结果不一致，包括２例ＰＣＲ阳性但培养阴性，１例培养阳性但ＰＣＲ阴性，将ＰＣＲ与细胞培养进行比较，ＰＣＲ的敏感性为８５．７％，特异性９１．３％，阳性预     

尿道粘膜印片检测沙眼衣原体

对１０９例前列腺炎病人尿道粘膜上皮印片进行检测，发现上皮细胞内沙眼衣原体包涵体２１例，革兰阴性双球菌１２例，衣原体包涵体和革兰阴性双球菌同时被检出者１５例，其余６１例二者均无。另外对３２例患前列腺炎、尿道炎及附睾炎的病人，分别作前列腺液涂片和尿道粘膜印片检查，结果发现，印片的阳性检出率明显高于前列腺液涂片的阳性率，提示临床医师应注意被检标本的采集。     

沙眼衣原体在男性泌尿生殖系的感染

近年沙眼衣原体引起的性传播性疾病增加较快,造成的泌尿生殖道感染的发病率已超出淋菌感染,成为最常见的性病之一.美国每年用于防治生殖系统沙眼衣原体感染的费用达10亿美元.现对沙眼衣原体的病原学、流行病学、诊断及治疗作一概述.1 病原学沙眼衣原体是一类介于病毒和立克次体之间的形态相似、能通过滤过器、严格细胞内寄生、有独特生活周期的原核细胞型微生物.沙眼衣原体对热敏感,其传染性在37℃48小时内显著下降,在60℃中10分钟完全丧失.沙眼衣原体在4℃能良好地存活     

正常男性尿道能否存在沙眼衣原体?--读"136例正常男性尿道内沙眼衣原体和支原体检测"一文有感

沙眼衣原体(chlamydia trachomatis,Ct)和解脲脲原体(ureaplasmauurealyticum,Uu)能引起非淋菌性尿道炎,而且是其常见的原因。但正常男性尿道中是否定植此种病原体?在“139例正常男性尿道内沙眼衣原体和支原体检测”一文中,作者检测了136例正常尿道发现,其中31.6%Uu阳性,15.4%C     

尿道粘膜印片检测沙眼衣原体

对１０９例前列腺炎病人尿道粘膜上皮印片进行检测，发现上皮细胞内沙眼衣原体包涵体２１例，革兰阴性双球菌１２例，衣原体包涵体和革兰阴性双球菌同时被检出者１５例，其余６１例二者均无。另外对３２例患前列腺炎、尿道炎及附睾炎的病人，分别作前列腺液涂片和尿道粘膜印片检查，结果发现，印片的阳性检出率明显高于前列腺液涂片的阳性率。提示临床医师应注意被检标本的采集。     

男性不育患者沙眼衣原体的检测

随着性传播疾病的增多，近年来性病与男性不育之间的关系正日益受到重视。为了解沙眼衣原体(CT)感染与男性不育的关系，我们应用连接酶链反应(LCR)法检测了男性不育患者尿液中CT，报道如下。     

淋菌性尿道炎合并砂眼衣原体感染的诊断和治疗

１９９３年８月－１９９４年６月，用微量酶联免疫分析方法自３２６例淋病患者检出１０２例砂眼衣原体感染者，阳性率３０．７％，其中男性５４例，阳性率２７．５％；女性４８例，阳性率３６．２％。对淋病合并ＣＴ感染较高的原因，症状，诊断和治疗进行了讨论。     

应用多聚酶链反应检测前列腺液中沙眼衣原体

从１９９４年１０月至１９９５年５月对７５例慢性前列腺炎患者应用多聚酶链反应（ＰＣＲ）技术检测其前列腺液中沙眼衣原体，并同时进行对照组的前列腺液检测。结果在前列腺液中沙眼衣原体阳性率为２６．７％，而且均为非细菌性前列腺炎。对照组均为阴性。前列腺按摩前初始尿和尿道脱落上皮细胞ＰＣＲ检测沙眼衣原体结果是前列腺液阳性者，尿及尿道上皮细胞均为阴性。本实验结果不仅表明沙眼衣原体是慢性非细菌性前列腺炎的病原体之一，而且为沙眼衣原体性前列腺炎提供了特异性诊断方法和治疗后监测手段。     

荧光探针定量聚合酶链反应检测684例女童泌尿生殖道淋球菌、沙眼衣原体、解脲支原体感染情况

目的了解女童泌尿生殖道淋病双球菌、沙眼衣原体、解脲支原体感染情况。方法对684例有泌尿生殖道感染症状的女童取阴道分泌物,采用荧光探针定量聚合酶链反应（PCR-荧光探针法）进行淋病双球菌、沙眼衣原体、解脲支原体检测。结果684例患儿19例淋病双球菌阳性,12例沙眼衣原体阳性,31例解脲支原体阳性。结论对于有反复泌尿生殖道感染的女童,临床上应考虑相关病原体感染的可能,建议进行此3种病原体的检查,以指导临床治疗。     

Acute and chronic urinary tract infections caused by Chlamydia trachomatis

Chlamydia trachomatis is one of the causes of acute and chronic urinary tract infections and acute or silent salphingitis. Chronic or recurrent female urinary or genital tract infections with Chlamydia trachomatis have been recognised as a significant factor in the development of acute or chronic renal interstitial inflammation or increased risk of ectopic pregnancy. In most cases Chlamydia trachomatis is sexually transmitted. Moreover, it is one of the most common sexually transmitted pathogens. The current estimate is that in the United States there occur 4.5 million new infections each year. We describe 3 cases of recurrent urinary tract infections due to Chlamydia trachomatis. This revised version was published online in September 2006 with corrections to the Cover Date.     

Chlamydia trachomatis infection as a problem among male partners of infertile couples

Chlamydia trachomatis infection is the most common bacterial sexually transmitted disease supposed to cause urethritis, epididymitis, prostatitis and infertility in men. The objective of this study was to assess the frequency of C. trachomatis infection in male partners of infertile couples at childbearing age. Sixty infertile couples and a control group of 40 healthy volunteers were included in the study. Urethral swabs were taken from all the male participants and cervical swabs from the female partners of the infertile couples. Culturing on McCoy cell line and PCR were the methods used for detection of the infection. C. trachomatis was found in five out of the 60 male urethral samples. Three of the female partners of these five positive males were diagnosed with C. trachomatis infection, too. We registered a woman with C. trachomatis infection whose partner's samples were negative for the bacterium. The control group showed one specimen positive for C. trachomatis . The frequency of C. trachomatis infection was 8.3% in the male partners of infertile couples at childbearing age when compared with 2.5% in the control group. It is most likely that infertility in the couples with chlamydial infection was due to the pathogen studied.     

男性不育患者解脲支原体及沙眼衣原体感染与抗精子抗体关系的研究

目的:探讨在男性不育症中解脲支原体(UU)、沙眼衣原体(CT)感染与抗精子抗体(AsAb)的关系.方法:应用荧光定量PCR技术检测105例不育男性精液中UU和CT,应用ELISA法测定血清中的AsAb.结果:不育男性血清AsAb阳性率 34.3%(36/105),UU阳性率 32.4%(34/105),CT阳性率 25.7%(27/105).AsAb阳性组中UU阳性率 47.2%(17/36),阴性组中UU阳性率 24.6%(17/69),两组差异有统计学意义(P＜ 0.05).AsAb阳性组中CT阳性率 27.8%(10/36),阴性组中CT阳性率 24.6%(17/69),两组差异无统计学意义(P＞ 0.05).结论:UU和CT感染是不育症患者的常见病因,而UU是AsAb产生的重要原因之一.     

Chlamydia trachomatis infection in male partners of infertile couples: incidence and sperm function

Chlamydia trachomatis infection is one of the most common sexually transmitted diseases. Its effect on male fertility, however, is still controversial. In this study, 284 male partners of infertile couples consulting the Center of Studies in Reproductive Biology (CEBRE) were analyzed. The incidence of C. trachomatis infection among male partners of infertile couples was 38.6%. There were no significant differences between infected and noninfected infertile men in any of the sperm parameters assessed (sperm concentration, motility and morphology). The results of the three bioassays developed to evaluate sperm physiology, namely spermatozoa-zona pellucida binding, acrosome reaction stimulated with human follicular fluid and zona-free hamster oocyte penetration, showed no differences between infected and noninfected men. Electron microscopy studies suggest that spermatozoa are active agents in the dissemination of the chlamydial infection; they could be acting as 'vehicles' for the pathogens. These, and other results, suggest that the possible effect of C. trachomatis on male fertility is not due to alterations in sperm 'quality' or function, but rather to the transmission of the disease to female partners, causing inflammatory processes and promoting the generation of antisperm antibodies.     

不育男性生殖道沙眼衣原体、解脲支原体感染与精子凋亡关系

目的 :探讨不育男性生殖道沙眼衣原体 ( CT)、解脲支原体 ( UU)感染与精子凋亡的关系。方法 :采用流式细胞术 ( FCM)检测生殖道 UU、CT感染者和未感染者精子凋亡情况。结果 :CT、UU阳性和阴性组精子凋亡率 ( PAS)分别为 ( 1 4 .54± 3 .90 ) %、( 8.3 7± 6.3 0 ) %、( 1 5.2 6± 6.56) %、( 9.2 5± 4.0 3 ) % ,CT+UU双阳性精子凋亡率高达( 2 2 .2 1± 6.52 ) % ,各组与正常生育组比较 ,差异非常显著 ( P<0 .0 1 )。阳性组与相应阴性组比较差异显著 ( P<0 .0 5)。结论 :生殖道 UU、CT感染可明显增加精子凋亡 ,提示引起男性不育的诸多因素可能因精子细胞凋亡所致。     

尿道灌注治疗衣原体和脲原体性尿道炎

目的：探讨乳糖红霉素和地塞米松尿道灌注治疗沙眼衣原体（ＣＴ）和（或）解腺原体（ＵＵ）性尿道炎的疗效。方法：筛选出１５０例不并发淋病、附睾炎及前列腺炎，有尿道炎症状，尿道分泌物荧光聚酶链反应检查ＣＴ和（或）ＵＵ阳性病例，随机分为３组，每机分为３组，每组各５０例。服药组，口服福爱力红霉素；灌注组，用１％乳糖红霉素和０．５％地塞米松作尿道灌注；对照组，尿道灌注生理盐水。１０ｄ为１个疗程。结果：经过１～２     

Chlamydial infection of the urinary tract in children and adolescents with hematuria

Urogenital infection with Chlamydia trachomatis in adults and adolescents is a common sexually transmitted disease. The purpose of this study was to investigate whether isolated microhematuria in children and adolescents is associated with Chlamydia trachomatis infection of the urinary tract. The study group included 37 children and adolescents with isolated nonglomerular microhematuria. Urethral smears for the isolation of Chlamydia trachomatis in cell culture were taken at the time of cystourethroscopy from all patients. Polymerase chain reaction (PCR) for the detection of chlamydial DNA in urine was carried out in 25 of 37 (68%) patients and direct immunofluorescence (DIF) of urine in 16 of 37 (43%) patients. The control group included 33 children and adolescents without hematuria; PCR and DIF of urine were carried out in all controls. Chlamydia trachomatis infection of the urinary tract was confirmed in 8 of 37 (22%) patients in the study group, and in none in the control group (0 of 33, P<0.001). Further studies of larger groups of patients should be conducted, before recommending testing for Chlamydia trachomatis infection of the urinary tract in children and adolescents with unexplained microhematuria. Received: 26 January 2000 / Revised: 26 April 2000 / Accepted: 2 May 2000