2型糖尿病与幽门螺杆菌感染

目的:探讨2型糖尿病与幽门螺杆菌(Hp)感染的关系。方法:采用14C呼气试验测定230例2型糖尿病患者Hp感染情况。结果:2型糖尿病患者的Hp阳性率为76%;且消化道症状如烧心、反酸、腹胀等的发生率明显高于Hp阴性组;其中有胃轻瘫者Hp感染率明显高于无胃轻瘫者;Hp阳性组糖尿病症状、糖尿病肾病、糖尿病周围神经病变发生率与Hp阴性组差异无显著性。结论:2型糖尿病患者Hp感染率高于正常人,合并有胃轻瘫者Hp感染率明显高于无胃轻瘫者,伴Hp感染的2型糖尿病患者消化道症状较明显,但对糖尿病症状及并发症发生无影响。     

根除幽门螺杆菌感染及血糖控制与糖尿病胃轻瘫症状改善的关系

目的本研究旨在探讨根除幽门螺杆菌(Helicobacter pylori,Hp)及血糖水平对糖尿病患者胃轻瘫症状的改善及影响因素。方法选择2012年3月~2014年6月于我科住院行Hp检测阳性的糖尿病患者424例,并行胃排空试验按是否有胃轻瘫分为A组及B组。采用标准抗Hp治疗,治疗后评估Hp根除疗效及胃轻瘫症状缓解情况。比较两组血糖水平,幽门螺杆菌根除率,以及不同血糖水平的患者幽门螺杆菌根除率及胃轻瘫症状缓解率。结果(1)与Hp阳性无胃轻瘫的糖尿病患者(B组)相比,Hp阳性合并胃轻瘫的糖尿病患者(A组)病程较长,空腹血糖、餐后2 h血糖及糖化血红蛋白(glycated hemoglobin A1c,Hb A1c)水平均较高(P均0.05);(2)Hp阳性合并胃轻瘫的糖尿病患者的Hp根除率为68.70%,成功根除后的患者胃轻瘫症状缓解率为77.51%,而未根除的糖尿病患者胃轻瘫症状缓解率仅为53.25%,两组比较差异有统计学意义(P0.05);(3)将Hp阳性合并胃轻瘫的糖尿病患者按Hb A1c水平分为三组,结果显示随着HbA1c水平的升高,Hp根除率下降,胃轻瘫症状改善率亦下降(P0.05)。结论 Hp根除对胃轻瘫症状改善有一定帮助,而血糖水平可影响Hp根除率及胃轻瘫症状的改善。     

2型糖尿病胃轻瘫与幽门螺旋杆菌感染的临床观察

目的探讨2型糖尿病与幽门螺杆菌（Hp）感染的关系。方法采用^14C呼气试验测定120例2型糖尿病患者Hp感染情况。结果2型糖尿病伴胃轻瘫患者的Hp感染的阳性例数为45例（男性18例,女性27例）,阳性率为77．6％,高于对照组（46．8％,P〈0．01）。结论2型糖尿病患者伴胃轻瘫者Hp感染率高于无胃轻瘫者。     

2型糖尿病胃轻瘫与幽门螺旋杆菌感染的临床观察

目的探讨2型糖尿病与幽门螺杆菌（Hp）感染的关系。方法采用胃镜下取活体标本检验Hp感染的方法来测定120例2型糖尿病患者Hp感染情况。结果 2型糖尿病患者的Hp阳性率（77.6%）高于对照组（46.8%,P〈0.01）,结论 2型糖尿病患者伴胃轻瘫者Hp感染率高于无胃轻瘫者。     

2型糖尿病与幽门螺杆菌感染的观察

目的探讨2型糖尿病与幽门螺杆菌（Hp）感染的关系。方法采用14C呼气试验测定180例2型糖尿病患者Hp感染情况。结果2型糖尿病患者的Hp阳性率（73%）高于对照组（53%,P〈0.01）;且消化道症状的发生率明显高于对照组（P〈0.01）。胃轻瘫者Hp感染率（88%）高于无胃轻瘫者（57%,P〈0.01）。结论2型糖尿病患者Hp感染率高于正常人,合并有胃轻瘫者Hp感染率高于无胃轻瘫者。     

2型糖尿病与幽门螺杆菌感染

为了解2型糖尿病幽门螺杆菌(HP)感染情况,我们对370例糖尿病患者进行幽门螺杆菌感染调查,并初步探讨HP感染与糖尿病胃轻瘫的关系.     

2型糖尿病患者幽门螺旋杆菌感染及其临床意义

目的:探讨2型糖尿病患者中幽门螺旋杆菌(HP)的感染率及临床意义.方法:将40例随机均分为糖尿病Ⅰ组(不伴胃轻瘫)、糖尿病Ⅱ组(伴胃轻瘫)和健康志愿者20例,用13C呼气试验(13C-UBT)法检测HP感染情况,计算各组感染率,分别与对照组进行比较,并做组间比较.结果:2型糖尿病患者HP感染率为60%,与正常人群感染率(45%)比较差异无显著性.2型糖尿病伴胃轻瘫患者HP感染率为77.8%,显著高于正常人群及糖尿病不伴胃轻瘫患者.结论:HP感染可能与2型糖尿病患者胃轻瘫的发生有关.     

幽门螺旋杆菌感染与2型糖尿病胃轻瘫发病的相关性研究

目的：了解幽门螺旋杆菌（HP）感染与2型糖尿病胃轻瘫发病的相关性。方法：将研究对象分为2型糖尿病伴胃轻瘫组（1组）、2型糖尿病不伴胃轻瘫组（2组）和健康人群组（3组）,每组各40例,进行HP检测,进行统计分析。结果：2型糖尿病伴胃轻瘫组HP感染率87.5%,2型糖尿病不伴胃轻瘫组HP感染率40%,健康人群组HP感染率22.5%。结论：糖尿病患者的HP感染率明显高非糖尿病患者,糖尿病伴胃轻瘫者HP感染率明显高于不伴胃轻瘫者,HP感染与2型糖尿病胃轻瘫的发病相关,糖尿病患者应定期检测HP,在胃轻瘫症状出现前进行干预,可以减轻患者的痛苦,节约医疗成本。     

针药结合对糖尿病胃轻瘫患者空腹血浆Ghrelin水平的影响

目的:通过观察糖尿病合并胃轻瘫症状患者空腹血浆Ghrelin水平的变化,探讨血浆Ghrelin与糖尿病胃轻瘫的关系及针药结合治疗对其的影响。方法:糖尿病无胃轻瘫症状患者35例为对照组;糖尿病合并胃轻瘫症状患者70例随机分为针药结合治疗组35例,莫沙比利治疗组35例。检测和比较针药结合治疗组和莫沙比利治疗组治疗28d前后血浆Ghrelin以及平均血糖(MBG)、平均血糖波动幅度(MAGE)、低血糖次数等指标的改变。结果:糖尿病胃轻瘫组患者的空腹血浆Ghrelin水平明显低于糖尿病无胃轻瘫组(P<0.05),其平均血糖(MBG)、平均血糖波动幅度(MAGE)、低血糖次数也明显高于糖尿病无胃轻瘫组(P<0.05);治疗28d后两治疗组平均血糖(MBG)、平均血糖波动幅度(MAGE)、低血糖次数均较治疗前有明显降低(P<0.05),空腹血浆Ghrelin水平高于治疗前水平(P<0.05),其中针药结合治疗组上述各指标的变化均大于莫沙比利治疗组(P<0.05)。结论:①糖尿病胃轻瘫患者存在血浆Ghrelin水平低下,可能与血糖波动等多因素有关;②针药结合治疗较单纯莫沙比利能更有效改善糖尿病胃轻瘫症状及减少血糖波动,其机...     

幽门螺杆菌感染与糖尿病胃轻瘫的关系研究

糖尿病患者常并发胃轻瘫,而幽门螺杆菌(HP)感染与糖尿病的关系近年已得到证实,其是否也参与了糖尿病胃轻瘫的发生,文献报道尚少,我们对此进行了研究。1资料和方法1.1研究对象2003年1月～2007年9月我院门诊及住院的HP阳性2型糖尿病患者68例,男32例,女36例,年     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.