梅毒患者与HLA—DRB1等位基因相关性研究

目的：检测山东汉族梅毒患者与HLA-DRB1等位基因的相关性。方法：应用聚合酶链反应一序列特异性引物技术（PCR—ssp）对196例山东汉族梅毒患者与500例山东汉族正常对照的HLA-DRB1等位基因表现频率进行检测。结果：患者组DRB1＊14等位基因的出现频率高于对照组（P〈0．05）;DRB1＊16等位基因的出现频率与对照组无显著差异（P〉0．05）。结论：HLA—DRB1＊14等位基因可能是梅毒的易感基因。     

银屑病的HLA等位基因及非HLA基因相关性研究进展

银屑病被认为是一种多基因遗传病。HLA是迄今为止所知的最复杂的人类遗传多态性系统，为基因学研究的理想标记物。银屑病与HLA等位基因及非HLA基因的相关性研究，提示银屑病易感基因可能位于HLA基因区内，或与HLA基因相连锁，亦可能HLA基因参与银屑病的发病。同时，银屑病与HLA等位基因的连锁不平衡在不同种族、地域存在明显差异，本文主要从HLA—Ⅰ、Ⅱ、Ⅲ类等位基因、单倍型及非HLA基因等几方面扼要介绍银屑病的遗传学研究进展，并进一步探讨银屑病的“保护因子”。     

山东汉族梅毒患者与HLA-A、B等位基因的相关性研究

目的 探讨山东汉族梅毒患者与HLA-A、B等位基因的相关性.方法 采用PCR-序列特异性寡核苷酸探针杂交(PCR-SSOP)方法对205例山东汉族梅毒患者与5844例山东汉族正常对照的HLA-A、B等位基因表现频率进行检测.结果 梅毒患者组HLA-A*02,B*15、40等位基因频率高于对照组(P值均＜0.01;Pc值均＜0.05),HLA-A*26等位基因频率低于对照组(P=0.003;Pc=0.039),HLA-B*15、40等位基因频率在显性梅毒组高于对照组(P值均＜0.01;Pc值均＜0.05),HLA-A*02、11、29,B*15、40等位基因频率在隐性梅毒组高于对照组(P值均＜0.01;Pc值均＜0.05),HLA-A*30、33等位基因频率在隐性梅毒组低于对照组(P值、Pc值分别为0.002、0.026;0.001、0.013),HLA-A*30等位基因频率在显性梅毒组高于隐性梅毒组(P=0.001;Pc=0.013).结论 HLA-A*02,B*15、40等位基因可能与山东汉族梅毒相关,HLA-A*30可能与山东汉族显性梅毒相关,HLA-A*02、11、29可能与山东汉族隐性梅毒相关.

Abstract：

Objective To investigate the association of HLA-A and -B alleles with syphilis in Shandong Han population. Methods The allele frequencies of HLA-A and -B were detected in 205 patients with syphilis and 5844 normal human controls by PCR-sequence specific oligonucleotide probe (PCR-SSOP)method. Results The patients with syphilis showed a higher frequency of HLA-A*02, B*15, B*40 alleles(all P＜0.01, Pc＜0.05) and a lower frequency of HLA-A*26 allele (P= 0.003, Pc = 0.039) than the normal human controls did. There was an increased frequency of HLA-B*15 and B*40 alleles in patients with symptomatic syphilis (both P＜0.01, Pc＜0.05), as well as an elevated frequency of HLA-A*02, 11, 29, B*15 and 40 alleles (all P＜0.01, Pc＜0.05) and a decreased frequency of HLA-A*30 and 33 in patients with asymptomatic syphilis(P=0.002, 0.026, Pc=0.001, 0.013 respectively), compared with the normal human controls. The frequency of HLA-A*30 allele was significantly higher in patients with symptomatic syphilis than in those with asymptomatic syphilis (P = 0.001, Pc = 0.013). Conclusions There seems to be an association between HLA-A*02, B* 15 and B*40 alleles and syphilis, between HLA-A*30 allele and symptomic syphilis, and between HLA-A*02, 11 and 29 alleles and asymptom1atic syphilis, in Shandong Han population.     

关节病型银屑病与HLA等位基因关联性Meta分析

目的:了解关节病型银屑病与HLA等位基因的关联性。方法:检索PubMed、ISI、中国期刊全文数据库(CNKI)、维普数据库(VIP)及万方数据资源系统(Wanfang database)1972年1月1日至2019年7月1日期间收录的有关关节病型银屑病与HLA等位基因关联性研究的相关文献,进行质量评估和Meta分析。OR≤0.8的HLA基因记为保护基因,OR≥1.2为风险基因,0.8相似文献   

白癜风的HLA等位基因研究进展

白癜风的病因尚不明,目前研究认为其发生与遗传因素相关,具有复杂的多基因控制的特点,尤其是与人类白细胞抗原HLA-Ⅰ类、Ⅱ类及Ⅲ类基因及其产物相关.此外,HLA内非Ⅰ、Ⅱ基因对疾病也有作用.HLA基因的多态性决定HLA分子的多样性.充分认识HLA基因水平的多态性,有助于白癜风的基因诊断和治疗.文中综述了近年来相关的研究进展.     

神经梅毒的研究进展

简要介绍近年来有关神经梅毒的发病机理，诊断和治疗的进展，神经梅毒诊断向两方面发展，一是改进实验室诊断方法，另一是应用新的射线诊断技术；治疗仍采用大剂量青霉素，疗后密切随访，目前尚无更有效的方法，强调合并ＨＩＶ感染时，神经梅毒诊治更为复杂，需慎重处理。     

白癜风的HLA等位基因研究进展

白癜风的病因尚不明，目前研究认为其发生与遗传因素相关，具有复杂的多基因控制的特点，尤其是与人类白细胞抗原HLA—Ⅰ类、Ⅱ类及Ⅲ类基因及其产物相关。此外，HLA内非Ⅰ、Ⅱ基因对疾病也有作用。HLA基因的多态性决定HLA分子的多样性。充分认识HLA基因水平的多态性，有助于白癜风的基因诊断和治疗。文中综述了近年来相关的研究进展。     

天疱疮与HLA

天疱疮的发病与遗传因素有关,特定人类白细胞抗原－Ⅱ类基因是天疱疮的易感基因。不同国家、不同种族的天疱疮易感基因具有相似性。人类白细胞抗原在天疱疮抗体的产生过程中有着重要作用。剔除或替换疾病易感基因将是天疱疮等自身免疫性疾病的治疗方向。     

尖锐湿疣与HLA抗原及HLA单倍型

尖锐湿疣与ＨＬＡ抗原及ＨＬＡ单倍型张庆瑞谷丽红杨德荣贺为东宋芳吉陈洪铎８０年代以来，尖锐湿疣（ＣＡ）的发病率明显增高，但人们发现并非所有的ＣＡ性伴或与ＣＡ患者有性接触者均临床发病；同时，也存在因非性接触而感染ＨＰＶ临床发病者［１，２］。这些现象表明，...     

梅毒疫苗研究进展

梅毒是由梅毒螺旋体感染引起的临床最常见的性传播疾病之一.近年来国内外梅毒发病率逐年升高,防控梅毒迫在眉睫.疫苗开发作为防控病原体感染最行之有效的手段,在梅毒研究领域得到越来越多的重视.本文综述近年来梅毒疫苗研究进展,以期为遏制梅毒传播提供帮助.     

寻常型天疱疮与细胞免疫关系的研究进展

天疱疮是一种以体液免疫介导的自身免疫性疾病,但随着免疫学和分子生物学技术的发展,体内和体外研究均发现天疱疮发病中同时存在细胞和体液免疫紊乱,自身反应性T细胞在其发病中起着重要作用。近几年研究重点已逐渐转向细胞免疫在天疱疮发病中的作用,但细胞免疫及细胞因子在天疱疮发病机制中的综合作用迄今还有待进一步阐明。该文从细胞因子的角度对细胞免疫与天疱疮的关系作一综述。     

梅毒的免疫学实验诊断进展

 梅毒被称为“伟大的模仿者”,其临床表现多样,病变部位广泛,单靠临床症状和体征诊断易造成漏诊或误诊,往往需要通过实验室确诊。梅毒螺旋体尚不能在体外进行长时间培养,病原体的形态学检查受样本取材等因素限制,灵敏度低。梅毒螺旋体感染后所产生的免疫反应成为确定梅毒螺旋体感染的重要依据。本文就传统免疫学试验在诊断梅毒方面的应用及新免疫学指标的进展作一综述,旨在为实验室结合自身条件及临床需求选择合适的梅毒免疫学检测方法提供依据。     

妊娠期梅毒的诊疗进展

梅毒螺旋体能通过胎盘引起胎儿宫内感染,造成流产、死产、早产或分娩胎传梅毒儿.死胎和新生儿死亡是最严重的妊娠不良结局.因此,围产期应常规开展妊娠期梅毒血清学的产前筛查工作.在梅毒高流行区或高危人群,尚需在孕28周和分娩时作2次血清学检查.一旦确诊,应尽早、足量、正规给予青霉素治疗,并加强新生儿随防.孕期有效的青霉素治疗,将降低围产期死亡率及胎传梅毒的发生.     

PPARs与皮肤病的关系研究进展

过氧化物酶体增殖物激活受体（PPARs）在过氧化物酶体增殖、脂肪生成、B氧化增加和细胞周期的调节等方面起着重要作用，它在皮肤生理及病理方面的作用逐渐受到重视。PPARct影响皮肤的成熟、分化和增殖，皮肤外伤后，PPARβ／δ一直保持表达直到创口愈合。PPARs对皮脂形成、皮肤肿瘤发展有不同的影响，在银屑病和色素性皮肤病中也起一定的作用。这些发现可为治疗皮肤病提供新途径。     

外周血T淋巴细胞亚群及Th17指标与梅毒的关系探究

目的:探究外周血T淋巴细胞亚群及Th17指标与梅毒的关系。方法:选取2014年2月至2015年12月于本院进行诊治的63例梅毒患者为观察组,63名同龄健康体检者为对照组,然后将两组的外周血T淋巴细胞亚群指标及血清Th17指标进行分别检测与比较,并比较观察组中不同分期与TRUST滴度梅毒患者的上述指标检测结果,另采用Logistic分析外周血T淋巴细胞亚群及Th17指标与梅毒的关系。结果:观察组的NK细胞均低于对照组,其他外周血T淋巴细胞亚群指标及血清Th17指标均高于对照组,且观察组中不同分期及TRUST滴度梅毒患者的上述指标检测结果也存在明显差异;采用Logistic分析显示,外周血T淋巴细胞亚群及Th17指标均与梅毒有密切的关系,P均0.05。结论:外周血T淋巴细胞亚群及Th17指标均与梅毒有密切的关系,因此对梅毒患者进行上述指标的检测价值较高。     

HLA antigens in patients with chronic biologically false-positive seroreactions for syphilis and systemic lupus erythematosus

Summary HLA antigens were examined in 118 unrelated patients of whom 49 had definite severe systemic lupus erythematosus (SLE). HLA A1 was found in 45% of the 49 SLE patients and in 20% of the controls, which consisted of 900 blood donors. HLA B8 was found in 47% of the patients and in 20% of the controls (relative risk=3.5, P=0.000017), while HLA B7 was found in 22% of the patients and in 24% of the controls.Of the 118 patients 69 had chronic biologically false-positive (CBFP) seroreactions for syphilis. In 32 of these 69 patients definite or probable SLE was diagnosed during the observation time, which varied from 3 to 16 years. HLA B7 was found in 38% of these 32 patients, while HLA B8 was found in 25%. When the occurrence of these antigens was correlated to the length of the observation time it was found that 53% of the patients in whom the disease manifested first after 10 years follow-up were carriers of HLA B7 (P=0.0005) and only 12% had HLA B8 (relative risk=0.5). In patients with a faster development of the disease both of these antigens were found in about the same frequency as in severe SLE patients.In 37 of the 69 patients no definite diagnosis could be established even it 19 of them had clinical and serological manifestations compatible with SLE. HLA B7 was found in 46% and HLA B8 in 14% of these patients. Moreover it was found that 72% of the 18 patients who had only antilipoidal antibodies in their serum were carriers of HLA B7, while 21% of the 19 patients with other circulating auto-antibodies had HLA B7. D-locus antigens were examined in 35 patients. HLA D w3 was found in 54% of the patients and in 24% of the controls. Twelve of the patients with D w3 had severe SLE and nine of them had also HLA B8.     

HLA and epidermolysis bullosa. Association between the HLA complex and recessive dystrophic epidermolysis bullosa

BACKGROUND AND DESIGN.--Epidermolysis bullosa refers to a group of genetic diseases characterized by marked skin fragility and blister formation following minor mechanical trauma. The patients with recessive dystrophic epidermolysis bullosa (RDEB) are the most severely affected with marked internal and external blistering, scarring, and death at an early age, secondary to malnutrition, septicemia, and/or metastatic squamous cell carcinoma. An association between RDEB and HLA antigens was explored in 28 patients with RDEB and their family members. RESULTS.--Our data demonstrate that susceptibility to develop RDEB may be associated with the HLA complex. The gene frequencies of DR4 and DQw3 were much higher in the patients than expected. These increases were likely due to statistically significant excess of DR4 and DQw3 homozygotes in the patients. In addition, the observed frequencies of two HLA haplotypes: Bw62, DR4, DQw3 and Bw60, DR4, DQw3 were significantly higher than expected. CONCLUSION.--If these observations are confirmed in the larger series of patients, the association between RDEB and HLA complex may have clinical utility in genetic counseling of siblings of child-bearing age who are at risk of being carriers of RDEB.     

The syphilis epidemic in Connecticut: relationship to drug use and prostitution

Syphilis rates in Connecticut increased four-fold between 1986 and 1988. During this time there were also signs of a large increase in cocaine use in the state. We studied links between these parallel trends in drug use and syphilis by examining two sources of data: information collected during syphilis case interviews and information from the syphilis screening program at the state's prison for women. As syphilis rates rose, there were large increases in the percentage of women with syphilis who reported prostitution or illicit drug use. In 1988, 41% of women with syphilis reported cocaine use, and 19% reported prostitution; 21% of male heterosexuals with syphilis reported cocaine use, and 31% reported sexual contact with prostitutes. Among incarcerated women, syphilis infection was frequent: of 113 women incarcerated for possession of illicit drugs in 1987-88, 7% were found to be infected with Treponema pallidum, and of 187 women incarcerated for prostitution in these years, 14% were infected. In both groups of incarcerated women studied, cocaine users had the highest syphilis rates, and those who administered drugs nonintravenously had rates similar to those who administered drugs intravenously. We concluded that the syphilis epidemic in Connecticut is related to the increase in use of illicit drugs (primarily cocaine) and that female drug users are at very high risk of syphilis regardless of whether they administer drugs intravenously or nonintravenously. We recommend that syphilis control efforts focus on wider serologic screening and early treatment of drug users, prostitutes, and their sex partners.