金花茶种子对体外培养不同肿瘤细胞增殖作用的影响

目的 观察金花茶种子95％乙醇提取物及95％乙醇提取物的乙酸乙酯部分、正丁醇部分、水溶部分3个极性部位对体外培养不同肿瘤细胞增殖作用的影响.方法 用RPMI1640培养基,常规传代培养人肺癌A549细胞、乳腺癌MCF-7细胞.采用噻唑蓝比色法(MTT)检测A549细胞和MCF-7细胞活性.结果 金花茶种子醇提取物部分、正丁醇部分和水溶部分对A549细胞和MCF-7细胞增殖均有抑制作用,其IC50分别为95.2,99.06,107.72 μg/ml;215.7,200.35,203.24 μg/ml,且均具有剂量依赖性的抑制作用.结论 金花茶种子95％乙醇提取物的正丁醇部分和水溶部分为其抗癌活性的有效部位,并且对A549细胞增值的活性更强.     

金花茶种子对α-葡萄糖苷酶活性的抑制作用研究

目的 研究金花茶种子乙醇提取物及醇提物的乙酸乙酯部分、正丁醇部分及水溶部分三个极性部位对α-葡萄糖苷酶的抑制作用。方法 采用体外抑制α-葡萄糖苷酶活性模型测定活性并进行酶抑制动力学研究,通过Lineweave-Burk作图法确定抑制类型。结果 金花茶种子乙醇提取物、乙酸乙酯部分、正丁醇部分及水溶部分均有抑制α-葡萄糖苷酶作用,其IC_(50)分别为37.74、335.90、20.53、17.80μg/ml,活性均高于阳性对照药阿卡波糖(484.90μg/ml)。其中阿卡波糖为竞争性抑制剂,其余均为非竞争性抑制剂。结论 金花茶种子为首次报道对α-葡萄糖苷酶有抑制活性,而正丁醇部分与水层部分为其抑制α-葡萄糖苷酶的有效部位。     

金花茶种子诱导人前列腺癌PC3细胞凋亡及其机制的研究

目的 探讨金花茶种子诱导人前列腺癌PC3细胞凋亡的机制.方法 用不同浓度金花茶种子乙醇提取物的正丁醇萃取物(CCEB)干预人前列腺癌PC3细胞,分别采用流式细胞仪、Western印记法等检测其对细胞凋亡及Bax、Bcl-2等表达的影响.结果 CCEB作用于人前列腺癌PC3细胞后,引起PC3细胞DNA片段化率增大,线粒体膜电位下降,细胞内caspase-3活性增高,Bax蛋白表达增加,Bcl-2蛋白表达减少,胞浆中细胞色素c含量增加.结论 CCEB诱导PC3细胞凋亡与caspase依赖性线粒体途径相关.     

当归提取物对PC3、HeLa、MDA-MB-435肿瘤细胞增殖的影响

目的:观察当归不同组分对人前列腺癌PC3细胞、人宫颈癌HeLa细胞、人乳腺癌MDA-MB-435细胞生长的影响。方法:采用当归水煎液和CO2超临界提取的当归油,分别与体外培养的人前列腺癌PC3细胞、人宫颈癌HeLa细胞、人乳腺癌MDA-MB-435细胞作用48 h后,用磺酰罗丹明B(SRB)染色方法检测细胞活性。结果:当归水提物(500~1 000μg/ml生药)与对照组相比,能够显著升高人宫颈癌HeLa细胞、人乳腺癌MDA-MB-435细胞的OD值(P<0.05),对人前列腺癌PC3细胞系列未见明显影响。超临界CO2提取的当归油,在3个细胞系列中与对照组相比能够明显降低OD值(P<0.01),并在0~250μg/ml范围内呈剂量效应关系。对3个肿瘤细胞系半数抑制浓度分别是218.24μg/ml、281.80μg/ml、285.60μg/ml抑制率最高可达到67%。结论:当归水提物有刺激人宫颈癌HeLa细胞、人乳腺癌MDA-MB-435细胞增殖的作用。当归油能够明显地抑制人前列腺癌PC3细胞、人宫颈癌HeLa细胞、人乳腺癌MDA-MB-435细胞增殖。结果提示,当归中能够刺激肿瘤细胞增殖的化合物可能存在于当归水溶性组分中。     

海绵提取物S1～S3抑制肝癌细胞和肺腺癌细胞增殖作用的实验研究

目的:用体外培养法研究海绵提取物S1～S3对肝癌7402细胞、肺腺癌GLC-82细胞和胃癌MCG-803细胞增殖的抑制作用.方法:采用MTT法进行研究.结果:海绵提取物S1、S2和S3分别处理肿瘤细胞48 h后,S3呈剂量依赖性抑制肺腺癌GLC-82和肝癌7402细胞增殖,测得IC50分别为2.714 μg/ml和2.598 μg/ml,而对胃癌MCG-803细胞增殖无明显抑制作用;S1和S2对3种肿瘤细胞增殖均无抑制作用.结论:海绵提取物S3对肝癌7402细胞和肺腺癌GLC-82细胞的增殖有抑制作用.     

桑黄提取物的抗肿瘤作用研究

目的 研究桑黄提取物对小鼠S180实体瘤、H22肝癌和Lewis肺癌实体瘤的生长抑制作用,体外对人瘤细胞的直接抑制作用.方法 建立小鼠S180实体瘤、H22肝癌和Lewis肺癌实体瘤模型,观察桑黄提取物高(1000mg/kg)、中(500mg/kg)、低(250mg/kg)剂量组体内抑制肿瘤生长作用,MTT法检测桑黄提取物抑制人肿瘤细胞增殖的作用.结果 ①桑黄提取物高、中、低剂量对小鼠S180实体瘤的抑制作用分别为27.9%、75%、31.2%;对小鼠H22肝癌的抑制率分别为46%、63%、57%;对小鼠肺癌Lewis的抑制率分别为57.1%、54.5%、45.9%.②体外对人瘤细胞的抑制作用:桑黄提取物给药剂量(20 μg/ml、40μg/ml、80μg/ml、160 μg/ml)对人卵巢癌细胞(A2780)的抑制率分别为16.9%、18.5%、25.8%、31.5%;对人乳腺癌(MCF7)细胞的抑制率分别为19.9%、28.1%、32.25%、30.8%.结论 桑黄提取物各剂量组对小鼠H22、S180、Lewis实体型肿瘤的生长均有抑制作用,体外对人肿瘤细胞MCF7,OVCA278有直接杀伤作用.     

越橘提取物抑制宫颈癌HeLa细胞增殖及诱导其凋亡的实验研究

目的 探讨越橘提取物抑制HeLa细胞增殖和诱导凋亡作用及其可能机制.方法 用浓度为0、0.025、0.25、2.5、25μg/mL的越橘提取物处理HeLa细胞24 h后.采用四甲基偶氮唑盐(MTT)方法检测越橘提取物对HeLa细胞增殖的抑制作用,采用Hoechst 33342/PI染色和DNA ladder方法观察越橘提取物对HeLa细胞凋亡的诱导作用,并采用Western blotring法检测caspase-3和caspase-8蛋白表达.结果 0.025、0.25、2.5和25μg/L的越橘提取物对HeLa细胞增殖的抑制率分别为22.81%、36.75%、42.62%和45.22%;0.25～25 μg/L的越橘提取物处理组细胞呈现明显的凋亡改变;Western blotring结果显示越橘提取物处理组细胞中caspase-8和caspase-3酶原蛋白激活,出现裂解片断.结论 越橘提取物可通过诱导HeLa细胞凋亡而抑制其增殖.     

夜香树花提取物体外抗肿瘤作用的实验研究

目的 探讨夜香树花提取物对体外培养的肿瘤细胞株生长抑制的影响.方法 按极性递增原则回流提取得到夜香树花提取物总浸膏和正丁醇部位,再采用MTT法和集落形成法观察夜香树花提取物总浸膏和正丁醇部位对人胃癌细胞株SGC7901、人肝癌细胞株Bele7404、宫颈癌细胞株Hela的生长抑制情况.结果 当夜香树花总浸膏浓度为40 μg/ml及正丁醇部位提取物浓度为20 μg/ml时对3种肿瘤细胞株的生长抑制率为48.10%~68.97%.结论 夜香树花提取物在体外具有明显抗肿瘤作用.     

茜草提取物对宫颈癌HeLa细胞杀伤动力学研究

目的:研究茜草提取物对宫颈癌HeLa细胞的杀伤动力学.方法:采用体外细胞培养方法,观察茜草提取物对HeLa细胞的杀伤作用.结果:观察结果表明,茜草提取物对HeLa细胞生长有明显的抑制作用,IC50为23.5μg/ml,其作用表现出时间依赖性.结论:茜草提取物有直接细胞毒作用,可用于恶性肿瘤的治疗.     

云南琵琶甲提取物抑制HeLa细胞增殖和诱导凋亡的研究

目的:研究云南琵琶甲粗提物对人子宫颈癌HeLa细胞的增殖抑制作用,并进一步探讨其提取物抑制肿瘤细胞增殖的机制。方法:用水提反复冻融法得到云南琵琶甲提取物,倒置显微镜观察、MTT法检测琵琶甲提取物对HeLa细胞的抑制作用,荧光染色观察、流式细胞术及琼脂糖凝胶电泳法检测药物诱导细胞凋亡的作用。结果:云南琵琶甲粗提物对HeLa细胞有明显的增殖抑制作用,IC50为413μg/mL,荧光染色可见细胞皱缩,染色质凝集并边缘化,琼脂糖凝胶电泳可以看到梯状条带,流式细胞术发现S期细胞增多。结论:确定云南琵琶甲提取物可抑制HeLa细胞增殖,并初步推测云南琵琶甲粗提物能够诱导HeLa细胞凋亡。     

Antitumor activities of dammarane triterpene saponins from Bacopa monniera

Bioassay‐guided methods were used to test the antitumor activity of methanol extract of the whole plant of Bacopa monniera (L.) Wettst. and four different fractions (petroleum ether, CHCl₃, EtOAc, and n‐BuOH fractions) of the methanol extract. Among the five crude samples, n‐BuOH fraction was noted to have the highest antitumor activity. The dammarane triterpene saponins isolated from n‐BuOH fraction, bacopaside É (1) and bacopaside VII (3), had potential antitumor effect. 1 and 3 showed cytotoxicity of all the tested human tumor cell lines MDA‐MB‐231, SHG‐44, HCT‐8, A‐549 and PC‐3M in MTT assay in vitro, and showed 90.52 % and 84.13 % inhibition in mouse implanted with sarcoma S180 in vivo at the concentration of 50 μmol/kg, respectively. The remaining two compounds, bacopaside II (2) and bacopasaponin C (4) were found to be much less potent compared with 1 and 3. 1 and 3 significantly inhibited human breast cancer cell line MDA‐MB‐231 adhesion, migration and Matrigel invasion in vitro at the concentration of 50 μmol/L. Since no antitumor activities about the monomers from Bacopa monniera (L.) Wettst. have been reported, these results indicate that the mechanism of action of 1 and 3 needs further study. Copyright © 2009 John Wiley & Sons, Ltd.     

正交实验优选金花茶种子总多酚的最佳提取工艺

目的 优选金花茶种子中总多酚的提取工艺.方法 采用丙酮-水法从金花茶种子中提取总多酚,利用正交实验优选总多酚最佳提取工艺,考察丙酮浓度、时间、温度、提取次数及料液比对总多酚收率的影响.结果 总多酚的最佳提取工艺为:25倍量的50%丙酮-水,80℃,提取3次,4 h/次,总多酚含量为9.59%,总多酚的收率为1.71%.结论 丙酮-水提取法可用于金花茶种子中总多酚的提取,其中提取溶剂倍量和提取温度对总多酚提取的影响较大.     

野八角的化学成分

目的:研究八角科Illiciaceae植物野八角Illicium simonsii茎叶的化学成分.方法:应用硅胶、Sephadex LH-20,Rp-C8,Rp-C18等各种色谱技术进行分离纯化,根据化合物的理化常数和波谱数据鉴定其结构.结果:从野八角茎叶的95%乙醇提取物中分离得到14个化合物,通过波谱数据和理化常数分别鉴定为ficusesquilignan A(1),醉鱼草醇C(2),醉鱼草醇D(3),leptolepisol A(4),acemikol(5),aviculin(6),山柰酚(7),槲皮素(8),槲皮素-3-O-α-L-吡喃鼠李糖苷(9),花旗松素-3-O-β-D-吡喃木糖苷(10),benzyl-2-O-β-D-glucopyranosyl-2,6-dihydroxybenzoate(11),2,4-dihydroxy-3,6-dimethyl-methyl-benzoate(12),biondinin C(13),莽草酸(14).结论:除化合物9,14外,其余化合物均为首次从该植物中分离得到.     

Antihyperglycemic activity of Selaginella tamariscina (Beauv.) Spring

Aim of the study

The present study was designed to investigate the effects of the EtOH and H₂O extracts of Selaginella tamariscina (Beauv.) Spring on hyperglycemia in diabetic rats and HepG2 cells, and to confirm the active fractions of EtOH extract in HepG2 cells.

Materials and methods

HepG2 cells and type II diabetic rats induced by low-dose streptozotocin (STZ) and high-fat diet (HFD) were used to evaluate the hypoglycemic effect of EtOH and H₂O extracts of Selaginella tamariscina. HepG2 cells were used to evaluate the promotive effect of different fractions of EtOH extract obtained from a polyamide column on glucose utilization.

Results

The results in HepG2 cells indicated that the EtOH extract had a better hypoglycemic effect than the H₂O extract. The results in diabetic rats indicated that both EtOH extract and H₂O extract were able to ameliorate the fasting blood glucose (FBG) level and improve oral glucose tolerance (OGTT). Total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-c), free fatty acids (FFA), tumor necrosis factor-α (TNF-α), alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and malondialdehyde (MDA) levels in serum were lowered. High density lipoprotein (HDL-c), insulin and superoxide dismutase (SOD) levels in serum were elevated as well as the hepatic glycogen content in diabetic rats. Compared with H₂O extract, the effects of EtOH extract were more marked. The 80% ethanol fraction exhibited a stronger hypoglycemic effect than the aqueous and 50% ethanol fractions, but the 95% ethanol fraction did not show any appreciable effects in HepG2 cells.

Conclusions

The results suggested that the EtOH extract had a better hypoglycemic effect than the H₂O extract; the 80% ethanol fraction from polyamide column had a strong hypoglycemic activity in HepG2 cells.     

Metabolite profiling and chemopreventive bioactivity of plant extracts from Bidens pilosa

Bidens pilosa has been used as a folk medicine in various medications and as a popular ingredient in herb teas. Chemopreventive activities of crude and fractionated plant extracts of Bidens pilosa were evaluated in this study. Ethyl acetate and butanolic fractions, partitioned from the total crude extract of Bidens pilosa, exhibited significant scavenging free radical activity (IC(50) values approximately = with 14-17 microg/mL) comparable to that of alpha-tocopherol. Strong effects on the inhibition of LPS-mediated nitric oxide production in RAW 264.7 cells were also observed for the EA and BuOH fractions. Detectable cytotoxicity on RAW 264.7 cells, however, was observed for the EA fraction at a dose >100 microg/ml. The metabolite profile and major constituents of the BuOH fraction were studied and characterized using various spectroscopic analyses. A new compound, heptanyl 2-O-beta-xylofuranosyl-(1-->6)-beta-glucopyranoside (1), and eight phenolic compounds, namely quercetin 3-O-rabinobioside (2), quercetin 3-O-rutinoside (3), chlorogenic acid (4), 3,4-di-O-caffeoylquinic acid (5), 3,5-di-O-caffeoylquinic acid (6), 4,5-di-O-caffeoylquinic acid (7), jacein (8), centaurein (9) were for the first time isolated from Bidens pilosa. Compounds 2-7 are the major antioxidative constituents in the Bidens pilosa extract.     

普洱茶籽的化学成分研究

普洱茶籽乙醇提出物的正丁醇萃取部分,经酸水解后,分离出5种成分,经光谱和化学分析,分别鉴定为二十八烷烃,柚皮素和3种新酯酰基皂甙元22-O-当归酸酯茶皂甙元B,22-O-当归酸酯茶皂甙元E和22-O-当归酸酯茶皂甙元A。     

Immunomodulatory activity of the rhizomes of Impatiens pritzellii var. hupehensis on collagen-induced arthritis mice

Impatiens pritzellii Hook. f. var. hupehensis Hook. f. (Balsaminaceae) has been well-known and widely used in China as an anti-rheumatoid arthritis (anti-RA) herb. In this present study, mice with collagen-induced arthritis (CIA) have been treated with the methanol (MeOH) extract (0.56, 1.12, 1.68 and 2.24 g/kg body weight) and the n-butanol (BuOH) fraction (0.13, 0.27, 0.40 and 0.53 g/kg body weight) of the rhizomes of Impatiens pritzellii orally for 3 weeks. The progression of CIA was evaluated by macroscopic scoring. Administration of the MeOH extract at dose of 1.12 g/kg and the BuOH fraction at 0.53 g/kg suppressed the development of CIA in mice significantly. The spleen and thymus indexes were measured and the levels of IgG, IL-10, INF-gamma and IL-18 in the serum of CIA mice were examined after the treatment of the MeOH extract (1.12 and 1.68 g/kg body weight) and the BuOH fraction (0.40 and 0.53 g/kg body weight). Administration of the MeOH extract and the BuOH fraction of Impatiens pritzellii decreased the spleen and thymus indexes, down-regulated the levels of IgG, INF-gamma, IL-18, and up-regulated the concentration of IL-10 in the serum of mice with CIA. From the results, it was concluded that administration of Impatiens pritzellii had obviously therapeutic effects on RA including immunomodulatory activity. Moreover, the BuOH fraction exerted the activity of anti-RA of Impatiens pritzellii.     

Scutellaria radix Extract as a Natural UV Protectant for Human Skin

Ultraviolet (UV) radiation induces oxidative injury and inflammation in human skin. Scutellaria radix (SR, the root of Scutellaria baicalensis Georgi) contains flavonoids with high UV absorptivity and antioxidant properties. The purpose of this study was to examine the potential use of SR extract as an additive in cosmetic products for UV protection. SR extract and its butanol (BuOH) fraction strongly absorbed UV radiation and displayed free radical scavenging activity against 2,2‐diphenyl‐1‐picrylhydrazyl radials and 2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) radicals. They also attenuated the UV‐induced death of HaCaT cells. Sunscreen creams, with or without supplementation of SR extract BuOH fraction, were tested in vivo in human trials to evaluate potential skin irritation and determine the sun protection factor (SPF). Both sunscreen creams induced no skin irritation. A sunscreen cream containing 24% ZnO showed an SPF value of 17.8, and it increased to 22.7 when supplemented with 5% SR extract BuOH fraction. This study suggests that SR‐derived materials are useful as safe cosmetic additives that provide UV protection. Copyright © 2015 John Wiley & Sons, Ltd.