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1.
Wang Jing Cheng Kai Qin Zhuo Wang Yanping Zhai Lijing You Min Wu Juanjiao 《中医杂志(英文版)》2017,37(1):96-100
OBJECTIVE
To investigate the effects of electroacupuncture (EA) at the Guanyuan (CV 4) or Sanyinjiao (SP 6) acupoints on the hypothalamus-pituitary-ovary (HPO) axis and spatial learning and memory in female mice.METHODS
Nine-month-old female mice with senescence-accelerated mouse prone 8 (SAMP8) were divided into three groups: the disease model, EA-Guanyuan and EA-Sanyinjiao groups. Concurrently, 9-month old female mice with senescence-accelerated mouse resistance 1 (SAMR1) were set as the control model group. The two treatment groups were given the same pattern of EA stimulation. Gonadotropin-releasing hormone, luteinizing hormone, follicle-stimulating hormone (FSH) and Serum estradiol levels in the Hypothalamus-pituitary-ovary axis were assessed by enzyme-linked immunosorbent assay to determine the HPO axis function level. Spatial learning and memory were assessed by the Morris Water Maze (MWM) test.RESULTS
(a) HPO axis: compared with the control model group, the disease model group displayed a decrease in E2 levels (P < 0.01), and an increase in GnRH, LH and FSH levels (P < 0.01). E2 levels were increased in EA treatment groups compared with the disease model group (P < 0.05). In contrast, GnRH and LH and FSH levels were reduced (P < 0.05). EA-Sanyinjiao group was superior than EA-Guanyuan group on increasing E2 and declining GnRH levels (P < 0.01). (b) The MWM test demonstrated that the response latency in the EA-Sanyinjiao treatment group declined from day 2 to day 5 compared with the disease model group (P < 0.05), whereas the EA-Guanyuan treatment group showed no significant difference.CONCLUSION
EA can regulate hormone (E2, FSH, LH, GnRH) levels in the HPO axis and the spatial learning and memory ability in female SAMP8 mice. Moreover, this effect may have been more pronounced in the EA-Sanyinjiao group than the EA-Guanyuan group. The underlying mechanism of the EA-induced changes may be related to gonadal hormone shifts in the HPO axis, followed by an improvement in spatial learning and memory. 相似文献2.
Wang Dan Ji Bo Zhao Guozhen Yan Mingna Bai Hongxin Sun Xiaomin Dai Jian Lu Yawen Liu Yitian You Min Wu Jiaojuan Ge Yunpeng Su Hang Cheng Kai 《中医杂志(英文版)》2017,37(6):794-803
Objective
To investigate the effect of electroacupuncture (EA) of acupoints Neiguan (PC 6) and Tianquan (PC 2) on the skin temperature, blood perfusion, and alpha1- and beta2-adrenoreceptor (α1-AR and β2-AR) protein and mRNA level in rats with acute myocardial ischemia.Methods
Thirty male adult Wistar rats [(230 ± 20) g] were randomly assigned into five groups (n = 6 each): a control group, sham operation group, model group, model group treated with EA at low frequency (L-EA, 2 Hz, 1 mA) and model group treated with EA at high frequency (H-EA, 100 Hz, 1 mA). The rat model was prepared by ligating the left anterior descending coronary artery. Electroacupuncture was performed at the left Neiguan (PC 6) for 20min daily for 3 d. After the 3rd time of the treatment, measurements of skin blood perfusion and temperature in Neiguan (PC 6) and Tianquan (PC 2) in all groups were made by laser speckle contrast imaging and infrared thermal image instrument. Real-time PCR and ELISA were used to measure mRNA level and protein level of α1-AR and β2-AR in the skin tissues of Neiguan (PC 6) and Tianquan (PC 2), respectively. Serum levels of cTnT and electrocardiogram were used to identify the state of myocardium.Results
In the group receiving electroacupunture at Neiguan (PC 6), compared with control group, the skin temperature, blood perfusion, and β2-AR mRNA and protein level of model group significantly decreased (P < 0.05), α1-AR mRNA and protein level of model group significantly increased (P < 0.05); compared with model group, the skin temperature, blood perfusion, and β2-AR mRNA and protein level of L-EA significantly increased (P < 0.05), α1-AR mRNA and protein level of L-EA and H-EA significantly decreased (P < 0.05). The same trend has taken place in the former four groups of the Tianquan (PC 2).Conclusion
Low-or high-frequency electroacupuncture can improve the skin temperature and blood perfusion which may be induced by decreasing the level of α1-AR and increasing the level of β2-AR of the Neiguan (PC 6) and Tianquan (PC 2) in the rat with acute myocaidial ischemia. 相似文献3.
Benoît Bataille Carine Chan-Shun Bastian Nucci Bernard Verdoux Michel Mora Pierre Cocquet Stein Silva 《中医杂志(英文版)》2017,37(4):554-557
Objective
To observe the effect of transcutaneous electroacupuncture (TEA) at Neiguan (PC 6) on refractory vomiting in critically ill patients in intensive care (ICU) setting.Methods
Ten patients admitted in ICU and presenting vomiting refractory to one or more antiemetic drugs were prospectively included in the study. TEA was applied at acupoint of Neiguan (PC 6) during 30 min with a neuromuscular transmission monitor (single-twitch stimulation with 1 Hz at a constant current of 10 mA). Nausea and Vomiting were evaluated at the following intervals: immediately after 30 min of TEA at Neiguan (PC 6), 30 min-6h and 6–24 h. The presence of nausea and/or vomiting throughout the observational period was defined as the primary end point.Results
The presence of nausea or vomiting throughout the observational period was 10% at the end of TEA, 40% between 30 min and 6 h, and 50% between 30 min and 24 h (P < 0.001, P = 0.01 and P = 0.03 vs pre-TEA, respectively). There were no complications or side effects related to TEA.Conclusion
TEA at Neiguan (PC 6) seems effective in reducing refractory vomiting in the patients in ICU setting, even if larger trials are needed to define optimal modalities. 相似文献4.
Fan Wentao Huang Yuwei Wang Lisheng Liao Weiguo Li Zhou Wu Yinai Liao Fengyun Yu Jianye Liu Qiang 《中医杂志(英文版)》2017,37(4):503-509
Objective
To investigate the effect of stimulating the acupoints Feishu (BL 13) and Dazhui (GV 14) on the transdermal uptake of sinapine thiocyanate contained in a gel used for the management of asthma.Methods
Thirty Sprague-Dawley rats were randomly divided into three equal groups using a random number table: the Feishu (BL 13) acupoint group, the Dazhui (GV 14) acupoint group, and the nonacupoint group or control group. Using microdialysis technology, preprocessed skin probes were implanted into the rats at Feishu (BL 13), Dazhui (GV 14), and a nonacupoint site. Asthma gel was then placed on the skin at Feishu (BL 13), Dazhui (GV 14) acupoints, and the nonacupoint for all groups. Dialysate was collected every 30 min for 12 h. The normalized concentration of sinapine thiocyanate in the skin was determined by high-performance liquid chromatography.Results
The rat in vivo transdermal experiment demonstrated that the quantity-time equation showed a good linear correlation with zero-order kinetics (r > 0.99). The transdermal behavior was in accordance with the first-order rate open model in which the transdermal penetration rates and the accumulative amounts of sinapine thiocyanate in the skin at the acupoint sites were greater than those through the skin of the nonacupoint site. The systemic maximum concentration and the area under the curve of sinapine thiocyanate in the acupoint groups were significantly greater than in the nonacupoint group. A lag time was observed in both acupoint groups, but not in the nonacupoint group.Conclusion
Stimulating the acupoints promotes the percutaneous absorption of sinapine thiocyanate and also controls its release, reducing concentration fluctuations in the blood. 相似文献5.
Li Jing Bai Zonglu Du Yuanhao Li Yongfeng Zhang Xuezhu Pang Bo Zhang Jingjing 《中医杂志(英文版)》2017,37(5):659-666
Objective
The aim of this study is identify the intervention mechanism of the effects of electro-acupuncture on the expression of Ang/Tie-2 mRNA and protein in rats with acute cerebral infarction induced by middle cerebral artery occlusion (MCAO).Methods
Altogether 120 Wistar rats were subjected to MCAO by inserting a nylon filament, and then divided into 3 groups: control group, injured group and electro-acupuncture group. The injured and electro-acupuncture groups were further divided into the following 7 subgroups according to the time after MCAO: 3, 6, 12, 24 h, 3, 7 and 12 day, with 8 rats in each subgroup. The electro-acupuncture group was given electro-acupuncture treatment at Shuigou (GV 26) instantly after operation. The rats were killed at different time points according to their groups, and then the expression levels of Ang/Tie-2 mRNA and protein were detected using Real-Time PCR and immunohistochemical staining.Results
The mRNA and protein expression levels of Ang/Tie-2 in the electro-acupuncture group were significant higher than that in the injured group.Conclusion
The results suggested that electro-acupuncture could significantly regulate the expression of Ang/Tie-2 mRNA and protein in the rats with acute cerebral infarction induced by MCAO, and enhance angiogenesis after ischemic penumbra. 相似文献6.
7.
She Yanfen Ma Liangxiao Zhu Jiang Qi Conghui Wang Yanxia Tang Ling Li Chunhua Yuan Hongwen Liu Yuqi Song Jiashan 《中医杂志(英文版)》2017,37(2):220-228
Objective
To assess skin temperature response to menstruation at acupuncture points in primary dysmenorrhea (PD) patients and healthy volunteers so as to explore acupuncture point specificity in reflecting diseases in the light of skin temperature.Methods
Fifty-two PD patients and 49 healthy volunteers were recruited. Skin temperature measurements were performed with a skin temperature assessment device at 10 points. Absolute difference between skin temperature of the same point on the left and right side is used as main outcome measure.Results
On the first day of menstruation, when menstrual pain attacking in PD patients, a significant increase in skin temperature difference was detected at Taixi (KI 3) compared with the healthy group (P < 0.01). A significant reduction in skin temperature difference was detected at Taixi (KI 3) in the first day of menstruation compared with those values in the third day after menstruation (P < 0.01) in the healthy group. On the third day after menstruation, a significant reduction in skin temperature difference was found at Zhongdu (LR 6) in PD group compared with the healthy group (P < 0.05). No significant differences of skin temperature were detected at other points (P > 0.05).Conclusion
The skin temperature difference at menstruation-relevant points in PD patients did not all change significantly more than those in women without PD. Significant difference was only found in Taixi (KI 3), the Yuan-source point of Kidney meridian. 相似文献8.
Yang Ling Lu Yuan Zhang Haifeng Ma Xiaopeng Bao Chunhui Wu Huangan Zhao Chen Ding Guanghong Qi Li Zhou Shuang 《中医杂志(英文版)》2017,37(4):538-545
Objective
To investigate the effects of warming moxibustion Tianshu (ST 25, bilateral) and Qihai (CV 6) in patients with diarrhea-dominant irritable bowel syndrome (D-IBS) by assessing predominant factors, and determining the best factor combinations and their interactions. To identify the optimal quantity of warming moxibustion Tianshu (ST 25, bilateral) and Qihai (CV 6) to achieve optimal efficacy.Methods
An L9 (34) orthogonal design was applied to 233 confirmed subjects based on the three most influential factors and the three most frequently used levels. Nine programs were designed. Subjects were assigned to four subgroups according to age and gender; each subject underwent one of the nine programs randomly. We selected Tianshu (ST 25, bilateral) and Qihai (CV 6). The scores of symptoms and the IBS Quality of Life (IBS-QOL) were evaluated after the final treatment.Results
After treatment, clinical symptoms of all patients were significantly improved (P = 0.001). Quality of life also improved significantly (P < 0.05). For all the participants, frequency was the dominating factor regarding symptoms (P = 0.01). Duration was the dominating factor for quality of life (P = 0.0001). The best combination for improving symptoms in 30–40-year-old male and female patients was two courses of 10 min each once a day, and for 41–50-year-old male and female patients it was three courses of 10 min (males) or 30 min (females) twice a day. The best combination for quality of life was three courses of 20 min once a day. No interaction was found between frequency and course, or frequency and duration.Conclusion
Warming moxibustion Tianshu (ST 25, bilateral) and Qihai (CV 6) is a promising therapy for D-IBS. To acquire optimal efficacy, the three main factors (duration, frequency and treatment course) of the warming moxibustion should reach a specific quantity and be combined appropriately. 相似文献9.
Objective
To evaluate the clinical efficacy and safety of five phase music therapy in patients with depression after ischemic stroke.Methods
A total of 92 patients with post-stroke depression were randomly divided into the control group (32 cases), treatment group A (30 cases), and treatment group B (30 cases). All groups were given basic therapies for cerebral infarction. In addition, the control group was administerd 50 mg of oral sertraline hydrochloride daily, while treatment groups A and B received needling at Baihui (GV 20) plus acupoint injection at Yanglingquan (GB 34) daily; treatment group B also received music therapy derived from the five phases in Traditional Chinese Medicine theory twice daily. All treatments were administered for 5 days per treatment cycle for three cycles, with a 1 day interval between cycles. In all three groups, Hamilton's depression scale (HAMD-17) score and the activities of daily life (ADL) score were measured before and after treatment, and side effects were assessed with the Treatment Emergent Symptom Scale.Results
The HAMD-17 score significantly decreased after treatment in all three groups, and the post-treatment reduction in HAMD-17 score was markedly greater in treatment group B than in treatment group A (P < 0.01). The ADL score significantly increased after treatment in all three groups, and the post-treatment increase in ADL score was significantly greater in treatment group B than in treatment group A (P < 0.01). The Treatment Emergent Symptom Scale score was highest in the control group, and lowest in group B, and significantly differed between the three groups (P < 0.01).Conclusion
Five phase music therapy plus acupoint needling and acupoint injection can improve the symptoms in patients with post-stroke depression. 相似文献10.
Li Hui Jin Rongjiang Yuan Kezhu Zheng Bo Zheng Zhong Luo Ying Ye Hua Huang Bingjie Zhu Tianmin 《中医杂志(英文版)》2017,37(1):43-48
OBJECTIVE
To observe the therapeutic effects of electro-acupuncture (EA) combined with psychological intervention on the symptom of somzatization or obsession and mental symptom of depression or anxiety and P50 of Auditory Evoked Potential (AEP) on internet addiction disorder (IAD).METHODS
One hundred and twenty cases of IAD were randomly divided into an EA group, a psycho-intervention (PI) group and a comprehensive therapy (EA plus PI) group. Patients in the EA group were treated with EA. Patients in the PI group were treated with cognition and behavior therapy. Patients in the EA plus PI group were treated with electro-acupuncture plus psychological intervention. Scores of IAD, scores of the symptom checklist 90 (SCL-90), latency and amplitude of P50 of AEP were measured before and after treatment.RESULTS
The scores of IAD after treatment significantly decreased in all groups (P < 0.05), and the scores of IAD in the EA plus PI group were significantly lower than those in the other two groups (P < 0.05). The scores of SCL-90 assembled and each factor after treatment in the EA plus PI group significantly decreased (P < 0.05). After treatment in the EA plus PI group, the amplitude distance of S1P50 and S2P50 (S1-S2) significantly increased (P < 0.05).CONCLUSION
EA combined with PI could relieve the mental symptoms of IAD patients, and the mechanism is possibly related to the increase of cerebrum sense perception gating function. 相似文献11.
Objective
To investigate the effects of needling the Shu, Yuan, and Mu acupoints on serum uric acid (SUA), xanthine oxidase (XOD), and alkaline phosphatase (ALP) levels and the kidney index in a rat model of gout and hyperuricemia.Methods
Fifty rats were randomly divided into five groups: blank, model, Shu-acupoint, Yuan-acupoint, and Mu-acupoint groups. A rat model of hyperuricemia was developed by intragastric administration of adenine and ethambutol. This experiment last for 90 d in total. Treatment groups underwent 3 courses of acupuncture. Each course involved a total of 10 interventions (one intervention every second day) with each intervention lasting 15 min. There was a break for 10 d between courses. SUA and ALP were analyzed using an automatic biochemical analyzer and XOD was analyzed using immunofluorescence.Results
Compared with the blank group, SUA and XOD levels in the model group were significantly higher and the renal index significantly improved. Compared with the model group, SUA and XOD levels in the three treatment groups decreased and the renal index significantly improved. When the three treatment groups were compared, the Mu-acupoint group showed the greatest decreases in SUA and XOD levels, followed by the Yuan-acupoint group. There was no significant difference in kidney index among the three treatment groups. There was no significant difference in ALP levels among the groups.Conclusion
The three treatments showed significantly reduced SUA and XOD levels compared with the control groups, possibly suggesting reduced renal damage. 相似文献12.
Lu Fengyan Gao Junhong Wang Yuanyuan Dai Qiufu Bao Yan Hong Yang Zhang Guolei Xin Juanjuan Zhao Yuxue Wu Shuyang Chen Yuanyuan Li Xiaojiao Fang Jiliang Yu Xiaochun 《中医杂志(英文版)》2017,37(3):298-307
Objective
To investigate the effects on the brain using three needling manipulations (twirling, lifting-thrusting, and twirling plus lifting-thrusting) when the right-side Zusanli (ST 36) acupoint was stimulated with needles.Methods
Seventeen healthy subjects accepted three needling manipulations stimulating the right Zusanli (ST 36) over separate days. Functional magnetic resonance imaging was used to detect changes in the brain during the manipulations, and then the needling sensations were recorded using the MGH acupuncture sensation scale (MASS) after each scan. fMRI data were processed using Statistical Parametric Mapping 8 to analyze the positive and negative activation in the brain induced by different acupuncture manipulations.Results
The individual needling sensations showed no statistically significant differences among the three manipulations. However, the MASS index showed that lifting-thrusting > twirling plus lifting-thrusting > twirling. Lifting-thrusting activated left premotor cortex, left postcentral, right middle frontal gyrus, left inferior frontal gyrus, right lingual gyrus, left insula, right putamen, bilateral cingulate gyrus and right cerebellum; and deactivated bilateral hippocampus and left caudate. Twirling activated bilateral orbital middle frontal gyrus, left opercular and triangular inferior frontal gyrus, and right middle occipital gyrus; and deactivated bilateral precuneus, right amygdala, left anterior cingulate gyrus, right inferior temporal gyrus, right middle frontal gyrus, right supplementary motor gyrus, and left postcentral. Twirling plus lifting-thrusting activated bilateral postcentral, left inferior occipital gyrus, left insula, left thalamus, left cingulate gyrus, and right putamen; and deactivated right superior frontal gyrus, right superior parietal gyrus, right temporal gyrus, right middle occipital gyrus, right insula, and left lingual gyrus. Pairwise comparisons of the three manipulations showed that signals induced by lifting-thrusting were the strongest, especially in the limbic system, followed by twirling plus lifting-thrusting; twirling alone was the weakest.Conclusion
Three methods of needling manipulations similarly activated areas associated with the somatosensory system, vision, cognition, and emotional regulation. This may have significant implications for acupuncture in clinical practice. 相似文献13.
Wu Lingling Liu Xiaohui Yin Yuzhu Sun Ke Wu Ling Yi Wei Li Shangrong Hou Hongying 《中医杂志(英文版)》2017,37(5):629-635
Objective
To evaluate the effectiveness of acupuncture analgesia (AA) compared with combined spinal-epidural anesthesia (CSEA) for labor pain relief and labor outcomes.Methods
We evaluated 131 primiparous women who received respiratory guidance during maternal uterine contractions and received either AA (n = 43), CSEA (n = 45), or no additional treatment (control, n = 43). The groups were compared regarding visual analog scale (VAS) scores for abdominal and back pain, and labor outcomes.Results
The abdominal VAS scores of the AA and CSEA groups were significantly lower than that of the control group. In addition, the VAS scores of the CSEA group were significantly lower than that of the AA group at 10 and 60 min after intervention. The back pain VAS scores of the AA and CSEA groups were significantly lower than that of the control group at 5, 10, and 60 min after intervention. The duration of the active phase of labor in the CSEA group was significantly longer than that of the AA and control groups. The rates of oxytocin use (4.70%), urinary retention (4.70%), and postpartum hemorrhage (273.72 ± 53.63 mL) in the AA group were significantly lower than in the CSEA group (46.70%, 24.20%, and 320.00 ± 85.60 mL, respectively).Conclusion
Both AA and CSEA were effective for labor pain relief, CSEA provided more effective pain relief, while AA was associated with a shorter duration of labor and fewer adverse effects.and each has its advantages and disadvantages. 相似文献14.
OBJECTIVE
To investigate the effect of brain functional recovery decoction (BFRD) on expression of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) protein in rats with cerebral ischemia reperfusion injury, and to explore the mechanism of action of BFRD.METHODS
Using the suture-occlusion method, a Wistar rat model of focal cerebral ischemia reperfusion was established. The rats were randomly divided into treatment group, model group, and sham operation group. The treatment group was administered BFRD. In situ hybridization was used to detect VEGF mRNA expression. Immunohistochemistry was used to observe expression of Ang-1 protein.RESULTS
VEGF mRNA expression was greater in the model group compared with the sham operation group (P < 0.05); Ang-1 protein expression was more obvious in the treatment group than the model group (P< 0.05).CONCLUSION
BFRD promoted VEGF mRNA and Ang-1 protein expression in the brains of rats with cerebral ischemia, suggesting increased angiogenesis. 相似文献15.
Akuodor Godwin Christian Ezeonu Chinonyelum Thecla Essien Augustine Dick Asika Ebere Chile Chilaka Kingsley Chimsorom Nwachukwu Daniel Ckukwu Nwobodo Ndubuisi Nwobodo 《中医杂志(英文版)》2017,37(4):431-435
Objective
To evaluate the in vivo antiplasmodial activity and the oral acute toxicity of the Bombax buonopozense root bark aqueous extract.Methods
The in vivo antiplasmodial activity of the root bark aqueous extract of Bombax buonopozense against early and established rodent malaria infections in chloroquine sensitive Plasmodium berghei strain in mice was investigated, and oral acute toxicity of the aqueous root bark extract of Bombax buonopozense was also evaluated in mice.Results
The findings of this study revealed significant (P < 0.05) and dose dependent decrease in parasitaemia in the parasitized groups treated with varying doses of the extract (50–200 mg/kg p.o.) in both suppressive and curative tests. There was also significant decrease in parasitaemia density in the chloroquine treated group. The aqueous extract was found no toxicity in mice and the oral LD50 was determined to be greater than 5000 mg/kg.Conclusion
Bombax buonopozense root bark aqueous extract possesses potent antiplasmodial activity and may therefore, serve as potential sources of new antimalarial agents. 相似文献16.
17.
Wang Hongwei Li Hailong Chen Feng qin Luo Jun Gu Jing Wang Huping Wu Hongyan Xu Yan 《中医杂志(英文版)》2017,37(2):229-235
Objective
To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action.Methods
Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 cells. Real time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of baicalin.Results
In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 µmol/L baicalin for 48 h, a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent manner. Moreover, baicalin up-regulated the expression of caspase-3, caspase-9, and B cell lymphoma (Bcl-2)-associated X protein and down-regulated the expression of Bcl-2 at both the mRNA and protein level.Conclusion
Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells. 相似文献18.
Moon Hwa Kwak Ji Eun Kim Jun Go Hong Joo Son Hee Seob Lee Jin Tae Hong Dae Youn Hwang 《中医杂志(英文版)》2017,37(4):475-485
19.