庚型肝炎病毒（GBV—C／HGV）与HBV和HCV联合或重叠感染的初步研究

目的 研究庚型肝炎病毒（ＧＢＶ－Ｃ／ＨＧＶ）与ＨＢＶ和ＨＣＶ联合或重叠感染的情况，方法 采用ＥＬＩＳＡ的方法检测血液制剂和血源乙肝疫苗原料血浆中的ＨＢｓＡｇ和抗－ＨＣＶ；并用５′非编码区的引物，用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）法检测ＧＢＶ－Ｃ／ＨＧＶＲＮＡ。结果 ＨＢｓＡｇ和抗ＨＣＶ均阴性血浆的ＧＢＶ－Ｃ／ＨＧＶＲＮＡ阳性率为１８．８％（１５／８０）；ＨＢｓＡｇ阳性而抗ＨＣＶ阴性血浆的ＧＢＶ－     

抗-HGV在各型病毒性肝炎患者中的检出情况及意义

目的了解抗庚型肝炎病毒抗体（抗－ＨＧＶ）在各型病毒性肝炎患者中的检出情况及临床意义。方法对１９０例不同病原的病毒性肝炎患者进行抗－ＨＧＶ（ＥＬＩＳＡ）检测,对其中抗－ＨＧＶ阳性者进行ＨＧＶ－ＲＮＡ（ＲＴＰＣＲ）检测。结果①在各型病毒性肝炎患者中总的抗－ＨＧＶ阳性率为１２１％（２３／１９０）。②ＨＣＶ感染者中抗－ＨＧＶ的阳性率（２６９％）最高,其后依次为ＨＥＶ（１８２％）,ＨＢＶ（１１２％）,ＨＤＶ（８３％）。③２３例抗－ＨＧＶ阳性的患者中仅有５例（２１７％）ＨＧＶ－ＲＮＡ同时检测阳性。④抗－ＨＧＶ阳性组与抗－ＨＧＶ阴性组比较,两组肝功能指标无统计学差异（Ｐ＞００５）。结论①抗－ＨＧＶ阳性并不一定代表ＨＧＶ病毒复制或现症感染。②ＨＧＶ致病性可能较弱。③ＨＧＶ可能通过多途径传播。     

HGV-RNA阳性与阴性慢性乙型肝炎患者的对比研究

目的 研究慢性乙型肝炎（ＣＨＢ）患者庚型肝炎病毒（ＨＧＶ）感染的意义。方法 ＲＴ＿ＰＣＲ法对１４６例ＣＨＢ患者血清进行了ＨＧＶ－ＲＮＡ检测,并将ＨＧＶ－ＲＮＡ阳性与阴性患者进行临床与病理学对比。结果 ＨＧＶ－ＲＮＡ阳性２３例（１５．７５％）。ＨＧＶ－ＲＮＡ阳性与阴性２肝功能等生化指标水平,肝脏病理损害程度、ＨＢＶ－ＤＮＡ阳性率均无显著性差异。５例ＨＧＶ－ＲＮＡ阳性、１６例ＨＧＶ－ＲＮＡ阴性ＣＨＢ患     

血清抗－HCV与HCV RNA检测结果比较

采用逆转录一套式－聚合酶链反应（ＲＴ－ｎｅｓｔｅ－ＰＣＲ）及ＥＬＩＳＡ（Ａｂｂｏｔｔ）对一组经第二代抗－ＨＣＶＥＬＩＳＡ（上海科华生物技术有限公司，科华）试剂筛选的抗－ＨＣＶ阳性血清及抗－ＨＣＶ阴性的非甲非乙型肝炎患者血清，进行了ＨＣＶＲＮＡ与抗－ＨＣＶ检测。对两者检测结果及相互关系进行了分析与比较。结果显示：Ａｂｂｏｔｔ与科华ＥＬＩＳＡ试剂两者抗－ＨＣＶ检测的阳性符合率及总符合率为７７．７％及７９．８％。两者与ＨＣＶＲＮＡ检测阳性结果的符合率分别为４９，７％及５６．３％。２０８份血清中ＨＣＶＲＮＡ阳性１０９份，其中抗－ＨＣＶ阳性组ＨＣＶＲＮＡ阳性率为６０．２％，较之抗－ＨＣＶ阴性之非甲非乙型肝炎ＨＣＶＲＮＡ阳性率（５０．０％）为高，其在ＰＣＲ试验中呈阳性与强阳性反应者的比率，前者亦显著高于后者（８５．２％与２５．ｏ％，Ｐ＜０．０１）。     

原发性肝癌患者血清和肝组织中庚型肝炎病毒基因的PCR检测

用逆转录－聚合酶链反应法（ＲＴ－ＰＣＲ）检测了原发性肝癌（ＰＬＣ）患者血清及肝癌和癌旁肝组织中的庚型肝炎病毒（ＨＧＶ）ＲＮＡ，以ＰＣＲ－双脱氧末端终止法测定了ＰＣＲ产物的核苷酸序列。结果显示，血清和肝组织中ＨＧＶＲＮＡ的检出率分别为１９．４％（１３／６７）和２５．７％（９／３５），且ＨＧＶＲＮＡ在肝癌组织呼吕旁肝组织中同时存在，血清和肝组织中ＨＧＶ ＲＮＡ检测结果的符合率为８５％；５′非编码区（５     

山东地区输血后丙肝患者HGV感染状况及HGV部分核酸序列测定

应用ＲＴ－ＰＣＲ法检测庚肝病毒（ＨＧＶ）ＲＮＡ,并对阳性扩增的产物采用ＰＣＲ片段直接克隆法测定。结果从８６例输血后丙型肝炎（ＰＴＨ－Ｃ）患者血清中检出ＨＧＶＲＮＡ阳性者３１例（３６．０％）,其中１例ＨＧＶＮＳ５区部分核苷酸序列与美国原始株和另１例日本株核苷酸同源性比较分别为８６．０％和８４．０％。证实山东地区ＰＴＨ－Ｃ２存在着ＨＧＶ感染和ＨＧＶ／ＨＣＶ混合感染,但是否存在不同亚型或ＨＧＶＲＮＡ阳性     

多重和套式聚合酶链反应检测血液,腹膜透析患者血清中HBV D…

利用免疫学和聚合反应（ＰＣＲ）方法，对３５例血液透析（血透），１４例腹膜透析（腹透）患者的８４份血清进行了检测。血透组抗－ＨＣＶ阳性率８２．９％，ＨＣＶ ＲＮＡ ＰＣＲ阳性率５１．４％；腹透组抗－ＨＣＶ阳性率仅７．１％，ＨＣＶ ＲＮＡ ＰＣＲ均阴性。ＨＢｓＡｇ和（或）ＨＢｅＡｇ在二组的阳性率分别为２５．７％和２１．４％；ＨＢＶＤＮＡＰＣＲ阳性率分别为４５．７％和６４．２％。透析患者ＨＣＶ和ＨＢＶ感     

血液病患者中庚型肝炎病毒检测及感染情况研究

目的：了解武汉地区血液病患者中庚型肝炎病毒（ＨＧＶ）的感染情况,探讨ＨＧＶ的传播途径。方法：采用酶联免疫吸附试验（ＥＬＩＳＡ）及逆转 录聚合酶链反应（ＲＴ－ＰＣＲ）方法测定各型血液病患者血清中的抗ＨＧＶ和ＨＧＶＲＮＡ。结果：５１例血液病患者中血清抗ＨＧＶ阳性６例,占１１．８％（５／６１）,ＨＧＶＲＮＡ均阴性。６例抗ＧＨＶ阳性患者中急粒２例,占２５％（２／８）,慢粒１例,占５０％（１／２）,再障２例     

丙型肝炎病毒抗体检测的诊断价值

用国产第二代丙型肝炎病毒抗体（抗－ＨＣＶ）检测试剂盒检测了２９６例临床血清标本以及２８例供血者血清标本。并用聚合酶链反应（ＰＣＲ）方法检测了这些标本的丙型肝炎病毒（ＨＣＶ）ＲＮＡ，以了解抗－ＨＣＶ检测对ＨＣＶ感染的诊断价值。同时探讨其在供血员筛选中的应用价值。结果在临床标本中，抗－ＨＣＶ阳性者有８１．１％ＨＣＶＲＮＡ阳性。抗－ＨＣＶ阴性者有２１．７％ＨＣＶＲＮＡ阳性。但是２８例抗－ＨＣＶ阴性的供血者ＨＣＶＲＮＡ全部阴性。提示：抗－ＨＣＶ阳性对于ＨＣＶ感染的诊断价值很高。临床怀疑为ＨＣＶ感染而进行检测的血清标本即使抗－ＨＣＶ阴性，也不能除外ＨＣＶ感染。用抗－ＨＣＶ筛选供血者可能使供血中ＨＣＶＲＮＡ阳性率大大降低。     

丙型肝炎病毒母婴传播的研究

目的研究丙型肝炎病毒母婴传播的可能性与机率。方法采用前瞻性研究方法，使用ＰＣＲ技术检测ＨＣＶＲＮＡ，用二代ＥＩＡ试剂检测抗－ＨＣＶ。结果１３例输血后丙型肝炎母亲和她们所生的１５例婴儿，婴儿ＨＣＶ感染率为８６．７％，其中临床型１例（７．７％），亚临床型ＨＣ３例（２３．１％），ＨＣＶ隐性感染９例（６９．２％）。抗－ＨＣＶ和ＨＣＶＲＮＡ阳性率均随年龄增长而降低，至３岁时分别为７．７％和１５．４％。结论感染ＨＣＶ母亲所生婴儿，ＨＣＶ感染率高，但转归较好。观察引产胎儿肝脏和血液发现，孕妇抗－ＨＣＶ和ＨＣＶＲＮＡ阳性所引产的胎儿血清和／或肝脏中抗－ＨＣＶ和ＨＣＶＲＮＡ阳性，ＨＣＶ的母婴传播，主要发生于子宫内。     

干扰素对HGV和HCV重叠感染中HGV的疗效研究

本研究通过探讨干扰素(IFN)对庚型肝炎病毒(HGV)和丙型肝炎病毒(HCV)重叠感染的慢性肝炎患者中HGV的疗效，以明确HGV感染的临床意义。利用逆转录多聚酶链反应(RT-PCR)，并对其产物直接进行测序。在IFN治疗期间，91．1％患者血中的HGV转阴。只有26．8％患者持续阴性。血中ALT水平在停用干扰素后与HCV-RNA水平密切相关，而与HCV-RNA的水平无明显关联。(1)HGV对IFN治疗敏感；(2)在HGV与HCV重叠感染的丙型肝炎患者中，HCV对肝细胞的损伤起重要作用，而HGV直接导致肝损伤且使肝损伤讲展的可能性很小。     

GBV-C/HGV and HCV infection in mixed cryoglobulinaemia

Recently, a new, suspected hepatotropic virus has been identified. Named GBV-C/HGV, this virus shares with the hepatitis C virus (HCV) routes of transmission and molecular organization. Indeed, a proportion of HCV-infected patients (10-25%) are also carriers of GBV-C/HGV. Since mixed cryoglobulinaemia (MC) is closely associated with HCV infection, the aim of this study was to determine the prevalence of GBV-C/HGV infection in MC patients, and to investigate whether the double infection influenced the clinical and/or laboratory aspects of the disease. 52 patients affected by MC were studied. 100 patients affected by HCV-positive chronic liver disease (CLD) without MC were used as control group. To determine the prevalence of GBV-C/HGV infection in general population, 150 blood donors were studied, as well as 80 patients affected by non-A-E CLD. Among the MC patients, only five (9.6%) were positive for both HCV and GBV-C/HGV infection. No difference was found between patients with and without double infection as regards main clinical and laboratory aspects. Among HCV-positive CLD cases, 27 were positive for double infection. Among blood donors, the prevalence of GBV-C/HGV infection was 8.0%, whereas in cases with cryptogenetic CLD the prevalence was 5.0%. In conclusion, these data show that GBV-C/HGV infection does not play any role in the pathogenesis of MC.     

GB virus C/hepatitis G virus in serum and liver of patients with chronic C and non B non C hepatitis: molecular and immunological aspects

GB virus C/hepatitis G Virus (HGV) is a single-stranded RNA virus that is transmitted parenteraly. This study investigates GB virus C in 62 patients with chronic hepatitis C (CHC) and non B non C hepatitis (CNBNC). The viral E2 protein was examined in the sera of the patients (using western blott assay) while viral replication in the liver was examined by detecting the negative strand of HGV-RNA and its E2 protein in liver tissue using in situ hybridization and immunohistochemical staining respectively. E2 protein was detected in 28% of patients with chronic hepatitis C and in 13.3% of patients with non B non C chronic hepatitis, while not detected in healthy blood donors (0%). HGV- E2 protein and the negative strand of HGV -RNA were detected in hepatocytes of only 3 out of the 13 examined liver biopsies from HGV infected patients (23%). The mean level of ALT in chronic HCV hepatitis patients who were +ve for HGV was significantly lower than those who were -ve for HGV. There was a significant difference between the mean value of HCV -RNA level by real time PCR in sera of hepatitis C positive patients with + ve HGV-E2 when compared with HCV patients with - ve HGV-E2 (p < 0.001). It is concluded that HGV co-infection may occur in some cases with CHC and CNBNC. Sites of replication, other than liver, are suggested as the virus was detected in liver tissue of only 23 % of cases inspite of its presence in their sera.     

庚型病毒性肝炎17例的临床和病原学分析

目的:对南京地区庚型病毒性肝炎的临床和病原学特点进行分析。方法:用反转录聚合酶链反应(RT-PCR)法检测血清HGV-RNA。从274例病毒性肝炎患者中检测出17例庚型病毒性肝炎,观察其临床表现及血清病原学标志,并分析庚型肝炎病毒(HGV)部分核酸序列。结果与结论:17例患者男性成年人多见,全年散发,经输血感染为重要传播途径。HGV可以单独感染,也可重叠(混合)其它肝炎病毒感染。少数为急性肝炎,多数为慢性肝炎或肝硬化,尤其在重叠(混合)感染。单纯HGV感染者症状轻,多隐匿发病,肝功能损害较轻。重叠(混合)感染者多有慢性肝炎的症状,与HBV重叠(混合)感染时有形成重型肝炎的趋势。核酸序列分析表明HGV南京株部分核苷酸序列与HGV美国株HGU 44402、HGU 45966、HGU 36380及HGV河北株在对应位置的核苷酸同源性从87.27%~93.94%,可能HGV有不同的基因型。     

Epidemiologic and virologic study of hepatitis C virus infections in Morocco

OBJECTIVES: We prospectively studied 783 consecutive Moroccan patients to define: 1) the prevalence of anti-hepatitis C virus (HCV) antibody (Ab), 2) the prevalence of other viral infections: HBs Ag, anti-HAV IgM, anti-HGV, HGV RNA, 3) the risk factors of spreading HCV infection, and 4) the distribution of HCV genotypes. RESULTS: 60/783 (7.7%) patients had anti-HCV Ab (48 H/12 F), 26 (3.3%) HBs Ag, and 3 (0.3%) IgM anti-HAV. Anti-HGV Ab was found in 11/60 (18.3%) anti-HCV positive patients, and 6/38 (15.8%) anti-HCV negative patients. 2/22 (9%) serum anti-HCV positive and anti-HGV negative patients were positive for HGV RNA. The 60 HCV positive patients rarely had other viral infections: 3 (5%) HBs Ag, 11 (18.3%) anti-HGV positive, 2 (9%) HGV RNA positive, and none had anti-HBc, IgM anti-HAV, or anti-HIV. HCV positive patients had more often undergone transfusion of blood products (21.7 vs 5.5%; P < 0.0001), and dental treatment (55% vs 8.3%; p < 0.0001). Patients with anti-HCV Ab frequently had hepatitis lesions on liver biopsy, i.e. chronic active hepatitis (n = 44) or cirrhosis (n = 16). HCV RNA was positive in 45/60 (75%) anti-HCV positive patients. HCV genotypes were: 1b (n = 21, 47%), 2a/2c (n = 13, 29%), 1a (n = 6, 13%), et 3 (n = 1, 2%). CONCLUSIONS: In our Moroccan population, the prevalence of HCV was high (7.7%). Other viral infections (HBV, HAV, HGV) were rare.     

原发性肝癌患者血清中庚型肝炎病毒核酸的检测分析

为了解庚型肝炎病毒（ＨＧＶ）在原发性肝癌患者中的感染状况，本文对１４５例原发性肝癌患者血清进行了分析。应用逆转录多聚酶链反应（ＲＴ－ＰＣＲ）技术扩增庚型肝炎病毒基因，采用蛋白酶Ｋ裂解法提取血清中ＨＧＶ－ＲＮＡ，逆转录为ｃＤＮＡ后进行巢式扩增，获得２３８ｂｐ的特异性片段。用此方法对１４５例原发性肝癌（ＨＣＣ）患者血清标本进行检测，结果发现２１例ＨＧＶ－ＲＮＡ阳性（１４．５％），均为男性患者。     

34例庚型肝炎病毒感染的临床特点

庚型肝炎病毒(HGV)是最近证实的一种新型肝炎病毒。我们以血清中检测到HGV RNA作为诊断HGV感染的指标,以了解HGV感染的临床特征。本文对34例HGV感染者进行临床分析,结果显示:在各类肝病人群中HGV总感染率为8.6％。在34例感染者中,有32例与其它类型肝炎病毒重叠感染。而且在各类急慢性肝病人群中均可有不同程度的HGV感染。HGV感染(含重叠感染)的临床表现各异,大多数症状较轻、恢复较快,但部分可导致肝病慢性化。HGV感染与肝硬变关系值得进一步探讨。     

单纯HGV感染急性肝炎患者的临床与病理研究

目的 通过对一组急性ＨＧＶ感染患者的肝组织病理和临床资料分析，探讨庚型肝炎病毒感染的致病性，方法 采用免疫组织化学技术对５９例不明原因包性肝炎患者的肝组织，进行乙、丙和庚型肝炎病毒怕检测，部分病例经原位杂交证实。结果 ＨＧＶＮＳ５怕的检出率为（５７．６％〈３４／５９），其中２０直组织中ＨＢｓＡｇ和／或ＨＣＶＮＳ３Ａｇ同时阳性ＩＨＧＶ合并感染组），ＨＧＶ阳性信号主要位于肝细胞浆内。ＨＧＶ单纯感染病例     

A cross-sectional study on HGV infection in a rural population

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