参景固本方体外抗氧化能力的研究

目的采用对DPPH自由基、超氧阴离子自由基和羟自由基的清除实验,对参景固本方的清除自由基能力和体外抗氧化活性进行评价。方法以维生素C(VC)为对照试剂,进行DPPH自由基、超氧阴离子自由基和羟自由基清除实验。结果参景固本方对DPPH自由基、超氧阴离子自由基和羟自由基均有不同程度的清除作用,在浓度为1 mg/m L时对DPPH自由基、超氧阴离子自由基和羟自由基的清除率达到了71.18%、66.58%、36.59%。结论参景固本方具有一定的抗氧化作用。     

芙蓉菊多糖体外抗氧化活性研究

目的 研究芙蓉菊多糖的体外抗氧化活性。方法 采用1,1-二苯基苦基苯肼（DPPH）自由基、还原力、超氧阴离子、羟自由基4种体外抗氧化模型研究芙蓉菊多糖的抗氧化活性,用维生素C作对照。结果 芙蓉菊粗多糖对DPPH自由基、还原力、超氧阴离子、羟自由基均有明显的清除能力,其中DPPH、超氧阴离子、羟自由基的EC50值分别为0.273、0.669、0.594 mg/mL,对羟自由基清除能力强于维生素C。芙蓉菊多糖对自由基清除率与其质量浓度存在着明显的量效关系。结论 芙蓉菊多糖具有良好的体外抗氧化活性,作为天然抗氧化剂和自由基清除剂有进一步研究的价值。     

碱蓬抗氧化活性成分的研究

目的从碱蓬中提取出醇溶性提取物、水溶性提取物和稀碱提取物,研究3种提取物的体外抗氧化活性。方法采用乙醇回流提取、热水煮提和稀碱提取法获得碱蓬提取物。利用超氧阴离子清除实验,羟自由基清除实验和DPPH自由基清除实验,研究3种提取物的抗氧化活性。结果水提取物有最强的羟自由基和超氧阴离子清除作用,且高于维生素C的清除能力。结论水提取物有较强的体外抗氧化作用,主要活性成分可能是多糖和醌类物质。     

玄参环烯醚萜类成分的体外抗氧化活性研究

[目的]研究玄参环烯醚萜类成分的体外抗氧化活性.[方法]采用溶剂萃取法,提取玄参环烯醚萜类成分.采用分光光度法,测定玄参环烯醚萜类成分对DPPH自由基、羟自由基、超氧阴离子的清除能力,以及对H2O2诱导的小鼠血红细胞氧化溶血的抑制能力.[结果]玄参环烯醚萜类成分能显著地清除DPPH自由基、羟自由基及超氧阴离子,可抑制H2O2诱导的小鼠血红细胞氧化溶血.[结论]玄参环烯醚萜类成分具有较强的体外抗氧化活性.     

仙人草中总黄酮体外抗氧化研究

目的 研究仙人草中总黄酮提取及体外抗氧化活性.方法采用超声辅助醇提法提取黄酮,测定黄酮对邻苯三酚自氧化产生的超氧阴离子自由基和羟自由基的清除率.结果 测定结果显示,黄酮对超氧阴离子自由基和羟自由基的清除率分别为54.85%和96.95%.结论黄酮对超氧阴离子自由基和羟自由基具有明显的抗氧化作用.     

山慈菇多糖的提取及其抗氧化作用的研究

目的 提取山慈菇多糖,测定其总糖含量并对其体外抗氧化能力进行初步研究.方法 采用热水浸提法提取山慈菇多糖,并通过脱蛋白,醇沉等方法分离纯化山慈菇多糖.采用苯酚-硫酸法测定山慈菇多糖的含量.通过邻苯三酚自氧化法、Fenton法和DPPH法测定山慈菇多糖溶液及对照组VC溶液在浓度分别为0.5mg/mL,1 mg/mL,2 mg/mL,4 mg/mL时对超氧阴离子、DPPH自由基及羟自由基的清除率.结果 苯酚-硫酸法测得分离纯化后的山慈菇多糖的浓度为30 mg/mL.山慈菇多糖溶液及VC清除超氧阴离子,DPPH自由基及羟自由基的效率随浓度的增加而增强,两组内差异具有显著性意义(P＜0.05,P＜0.001).结论 山慈菇多糖在体外具有明显清除超氧阴离子、羟自由基和DPPH自由基的作用,并且在体外的抗氧化作用均随其浓度的增大而增大.     

海带褐藻多糖硫酸酯的抗氧化活性研究

目的 研究海带褐藻多糖硫酸醋的抗氧化作用。方法 采用体外实验研究海带褐藻多糖硫酸醢对超氧阴离子、羟自由基、DPPH的清除作用以及对H2O2诱导的红细胞氧化溶血和大鼠肝匀浆脂质过氧化的保护作用。结果 海带褐藻多糖硫酸醋对超氧阴离子具有良好的清除作用，IC50为20．3μg／mL，其对羟自由基的清除作用较弱，对有机自由基DPPH的作用很弱。褐藻多糖硫酸醋能够抑制H2O2诱导的红细胞氧化溶血，对FeSO4-抗坏血酸体系造成的脂质过氧化具有良好的保护作用。结论 海带褐藻多糖硫酸醋具有显著的体外抗氧化活性。     

山茱萸果核不同提取物体外抗氧化活性

目的:对山茱萸果核不同提取物的体外抗氧化作用进行评价。方法:通过测定山茱萸果核石油醚、乙酸乙酯、无水乙醇和水提取物清除羟自由基(·OH)、超氧阴离子自由基(O_2~-·)及1,1-二苯基-2-三硝基苯肼自由基(DPPH·)的能力,评价其抗氧化活性。结果:山茱萸果核石油醚、乙酸乙酯、无水乙醇、水提取物和VC体外清除羟自由基的IC_(50)值分别为3.860、4.578、2.900、2.222、0.46 mg;清除超氧阴离子自由基的IC_(50)值分别为4.345、4.329、4.278、4.258、0.36 mg;果核乙酸乙酯、无水乙醇、水提取物和VC清除DPPH自由基的IC_(50)值分别为0.130、0.137、0.026、0.029 mg。结论:山茱萸果核的水提取物具有良好的抗氧化活性,具有潜在的开发应用价值。     

不同来源菊花总黄酮含量及其抗氧化活性研究

目的:测定不同来源菊花中总黄酮含量及其抗氧化活性。方法:采用Na NO2-Al(NO3)3-Na OH显色法测定总黄酮含量,利用DPPH自由基、羟基自由基和超氧阴离子自由基的抗氧化活性指数,以确定在菊花总黄酮清除自由基的能力。结果:不同来源菊花总黄酮含量差异较大,且均具有清除DPPH自由基、羟自由基和超氧阴离子自由基的能力,因菊花来源不同抗氧化活性能力大小也不同。菊花中总黄酮含量和DPPH自由基、羟基自由基的清除活性有明显的相关性,相关系数分别为0.660和0.518(P<0.01)。结论:不同来源菊花之间总黄酮含量存在差异,均具有较好的自由基清除活性,且总黄酮含量与抗氧化活性之间存在良好的量效关系。     

苯甲基异硫氰酸盐抗氧化作用体外实验研究

目的:研究苯甲基异硫氰酸盐的体外抗氧化作用。方法:用DPPH法和AP-TEMED(过硫酸铵-N,N,N’,N’-四甲基乙二胺)法对苯甲基异硫氰酸盐的抗氧化作用进行测定。结果:苯甲基异硫氰酸盐能明显清除1,1-二苯基苦基苯肼自由基(DPPH.),清除超氧阴离子自由基(O2-)作用较弱。结论:较高浓度的苯甲基异硫氰酸盐在体外能有效清除自由基,具有抗氧化活性。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.