流式细胞仪测定成人急性白血病患者DNA含量的临床意义

为探讨急性白血病患者骨髓细胞的周期分布，非整倍体，及其与临床疗效的关系。作者应用流式细胞仪测定了５７例ＡＬ患者的骨髓细胞ＤＮＡ含量。不同类型ＡＬ患者骨髓细胞Ｓ％均明显低于正常对照和缓解后患者。急性早幼粒细胞白血病Ｓ％最低；ＡＬ患者骨髓细胞ＤＮＡ非整倍体检出率２９．８％，４例完全缓解的患者仍检出ＤＮＡ非整倍体细胞，随化疗进展而消失。     

用DNA倍体分析乳腺肿瘤细胞的临床意义

目的　评价DNA倍体及细胞周期分析在乳腺肿瘤诊断上的价值。　方法　采用流式细胞术检测 5 0例乳腺肿瘤的细胞DNA含量和细胞周期。　结果　 10例纤维瘤都为二倍体、且CV <4 .0 % ;4 0例乳腺癌的二倍体发生率占 35 % ,CV >5 .0 % ,异倍体占 6 5 % ,两者差异有显著性 (P <0 .0 1)。乳腺癌组DNA指数 (DI)、S期细胞比率 (SPF)、细胞增殖指数 (PI)、DNA量值分布、G0 / 1峰值 (CV)均高于正常组 ,两者差异有显著性 (P <0 .0 1) ,乳腺癌DI值随肿瘤组织学分级的增高而增高。　结论　异倍体、SPF、PI、DI、G0 / 1峰值 (CV)的增高在乳腺良恶性肿瘤鉴别诊断中具有重要意义。     

DNA含量的流式细胞仪检测在急性髓细胞系白血病中的意义

目的:探讨DNA含量对急性髓细胞系白血病(AML)预后的影响.方法:用流式细胞仪对133例AML患者的骨髓或外周血单个核细胞DNA含量进行测定,分析与顸后的关系.结果:133例AML异倍体的检出率34.6%,DNA含量为异倍体患者的复发率略高于整倍体者,S G2M期百分比增高者治疗缓解率低,易早期复发.结论:DNA异倍体及S G2M期的百分比增高与AML的预后有关;流式细胞仪检测DNA含量,方法简单、快速,可作为筛检DNA异倍体的有效手段.     

Grading of malignancy of thymomas by flow cytometry]

The nuclear DNA contents of paraffin embedded archival tissues from 33 cases of thymoma and carcinoma of the thymus were studied by flow cytometry (FCM). Twelve cases were ruled out because of coefficients of variance (CV) > 10.0, leaving 21 cases for analysis. Included were 9 cases of non-invasive thymomas and 12 cases of invasive thymomas and thymic carcinomas. Among non-invasive thymomas, the DNA content was diploid in 4 cases and aneuploid in 5 cases. The DNA content was aneuploid in all 12 cases of invasive thymomas and thymic carcinomas. Proliferative activity, determined by measuring S phase percentage and proliferative index, was significantly increased in invasive thymomas and thymic carcinomas as compared with the non-invasive ones. The average S phase percentage for invasive thymomas and thymic carcinomas was 29.17 +/- 6.91, and for non-invasive thymomas it was 15.86 +/- 3.17 (P < 0.001). The average proliferative indices in the invasive and non-invasive groups were 47.76 +/- 5.03 and 32.58 +/- 6.58, respectively (P < 0.001).     

用流式细胞术检测人肿瘤组织细胞多药耐药基因表达产物P170

为研究检测人肿瘤组织细胞多药耐药基因表达产物，采用间接标记法，用鼠抗人Ｐ１７０单克隆抗体，二抗为ＦＩＴＣ标记兔抗鼠Ｉｇ，应用流式细胞仪检测了２９例新鲜实体肿瘤组织和一株多药耐药的Ｋ５６２细胞株。结果显示：１８例肿瘤细胞表达Ｐ１７０，其阳性率为６２．１％，１１例未检出表达，其阴性率为３７．９％；９９．９％Ｋ５６２细胞表达Ｐ１７０。在１８例Ｐ１７０阳性率高，但表达Ｐ１７０肿瘤细胞表达百分比低。检测肿瘤     

咽异感症405例病因分析

目的研究初步诊断咽异感症病因的方法。方法回顾分析405例迁延不愈的咽异感症患者临床资料。结果405例咽异感患者中,232例次部位恒定,173例次部位游走不定;201例伴失眠多梦,71例伴月经紊乱,97例伴胸闷心慌,129例伴上消化道疾病。痊愈270例（66．67％）,有效101例（24．94％）,无效34例（8．39％）,有效率91．60％。结论临床上依据咽异感症的伴随症状及部位作出初步诊断具有一定的可行性,但由不同病因引发的伴随症状之间存在交叉性,抑或多种病因混合存在,因此,明确诊断咽异感症病因仍有其局限性。     

流式细胞术测定先兆子痫产妇血小板活化

目的 探讨先兆子痫患者体内静态血小板与活化态血小板膜糖蛋白CD41a、CD62p、CD63表达量的变化及其临床意义。方法 采用全血免洗免疫直标法，用血小板CD41a、CD62p、CD63荧光单抗分别标记20例正常人、15例正常孕妇及16例先兆子痫产妇静息态与ADP活化后血小板，在流式细胞仪上测定其表达量。结果 先兆子病产妇组静息态与活化态血小板膜糖蛋白CD62p、CD63表达量均较正常孕妇组显著增高，CD41a表达无显著性差异；而后者与正常对照组比较，静息态间及活化态间血小板膜糖蛋白CD62p、CD63、CD41a表达量均无显著性差异。结论 先兆子病产妇体内血小板膜糖蛋白CD62p、CD63表达处于较高水平，提示血小板处于活化状态。     

Standard and Quantitative Analysis of Cyclin E Threshold by Cyclin E／DNA Multiparameter Flow Cytometry

E-type cyclins (cyclin E1 and cyclin E2) areexpressed during the late G1 phase of the cell cycleuntil the end of the S-phase . The activity of cyclinE canli mit the passage of cells throughthe restric-tion point“R”which marks a“point of no return”for cells entering the division cycle from a restingstate or passing from G1 into S-phase[1].In manyhuman tumors ,the expressionlevels of cyclin Eissignificantly higher than physiological ones .Cyclins/DNA multiparameter flow cytometrydetects…     

405例人体肿瘤细胞DNA含量的流式细胞术检测分析

为探讨不同类型的人体肿瘤细胞周期中 Ｄ Ｎ Ａ 含量与肿瘤组织学间的关系,采用流式细胞术检测了４０５例新鲜肿瘤组织中瘤细胞的 Ｄ Ｎ Ａ 含量,并用光学显微镜观察了肿瘤组织的病理改变。结果显示： ２２例良性肿瘤及３８３例恶性肿瘤中异倍体出现率分别是２７％ 及５２％ ;不同组织类型的恶性肿瘤中异倍体的出现率以腺癌为最高,其中肝癌、肠癌及胃癌异倍体的出现率分别为６１％ 、５５％ 及５１％ ,肉瘤次之,为３７％ ,鳞癌最少,为１７％ ;细胞增殖周期 Ｓ≥１０、 Ｇ２／ Ｍ ≥１０或 Ｓ≥２０、 Ｇ２／ Ｍ ≥５仅在恶性肿瘤中出现,良性肿瘤中无１例达到此值。８例多倍体肿瘤全是恶性。由此提示良性、恶性及不同组织类型的恶性肿瘤异倍体出现率有明显区别（ Ｐ＜ ０．０５）; Ｓ期细胞比率（ Ｓ Ｐ Ｆ）及 Ｇ２／ Ｍ 达到一定数值时,良恶性肿瘤有明显的区别（ Ｐ＜ ０．０１）。     

纵隔病变405例临床病理分析

目的：探讨纵隔病变的临床病理特征。方法：对1967－1997年外科切除的405例纵隔病变组织进行分析。结果：男216例,女189例,中位年龄26岁;肿瘤及瘤样病变230例（56．8％）,重症肌无力（MG）172例（42．5％）;在肿瘤性病变中,胸腺肿瘤113例（49．1％）居首位,平均年龄40岁;生殖细胞瘤36例（15．7％）,神经源性肿瘤30例（13％）,淋巴瘤仅6例（2．6％）,囊肿29例（12．6％）。前纵隔肿瘤165例（71．7％）,主要为胸腺肿瘤;中纵隔肿瘤6例;后纵隔肿瘤30例,主要系神经源性肿瘤。非肿瘤性病变中,MG胸腺淋巴滤泡性增生145例（84．3％）,无增生者27例（15．7％）;MG伴胸腺肿瘤38例（22．1％）,其中微小胸腺瘤12例（6．9％）。结论：MG胸腺伴有淋巴滤泡性增生者,预后好于胸腺非增生和胸腺瘤者,MG胸腺病理诊断对于发现微小胸腺瘤具有一定重要性。纵隔内胸腺肿瘤几乎占肿瘤病变的半数,国人淋巴瘤发病率较低,多数（84．3％）MG患者胸腺表现淋巴滤泡性增生。     

早期细胞凋亡的流式细胞术分析

目的：探讨多参数流式细胞术分析肿瘤细胞早期凋亡的方法。方法：采用Annexin V FITC／PI双染法及JC-1染色法标记食管癌EC6细胞，通过多参数流式细胞术分析X射线、顺铂诱导产生的细胞凋亡情况及线粒体膜电位的变化。结果：随着剂量的增加，凋亡细胞的百分率逐渐增加，线粒体膜电位下降，去极化细胞增多，并且有明显的剂量依赖性。结论：多参数流式细胞术具有广阔的应用前景，短时间内能够获得大量统计学意义上可靠的数据，为探讨细胞凋亡机制、寻找治疗靶点提供了有益线索。     

流式细胞术在子宫内膜疾病DNA分析中的应用

目的:探讨流式细胞术在子宫内膜疾病DNA分析中的临床应用价值。方法:应用流式细胞术对不同病变程度的石蜡包埋及新鲜的子宫内膜细胞DNA倍体含量和细胞周期时相进行分析。结果:子宫内膜增殖症组的异倍体率与正常对照组间的差异无统计学意义(P>0.05);与高分化子宫内膜癌组和低分化子宫内膜癌组间的差异均有统计学意义(P<0.05);DI、SPF介于正常对照组与高分化子宫内膜癌组之间,且与各组之间的差异均有统计学意义(P<0.05),各组细胞的DI、SPF值彼此有交叉现象;各组石蜡包埋的子宫内膜组织与新鲜子宫内膜组织之间的异倍体率和SPF差异均无统计学意义(P>0.05)。结论:流式细胞术DNA测定在子宫内膜病变诊断和评估预后中具有重要价值,而新鲜的子宫内膜流式细胞术分析操作更加简便、快速。     

流式免疫表型分析在11例侵袭性NK细胞白血病中的诊断价值分析

目的：探讨流式细胞术(flow cytometry,FCM)免疫分型及临床和实验室特征对侵袭性NK细胞白血病(aggressive NK-cell leukemia,ANKL)的诊断价值。方法：回顾性分析2019年1月至2019年12月在四川大学华西医院确诊的11例ANKL患者临床资料,包括骨髓细胞形态学、流式免疫表型及病理结果。结果：所有患者均出现发热症状并均伴噬血细胞淋巴组织细胞增生症(hemophagocytic lymphohistocytosis,HLH)。8例骨髓涂片中可见噬血细胞。流式细胞学示11例ANKL患者免疫表型均为：CD2⁺sCD3^-CD4^-CD8^-CD56⁺CD19^-,CD45和CD56表达强度增高(P=0.048)、细胞体积偏大(P=0.000),5例CDl6^-,6例CD7表达缺失或部分缺失。8例行免疫组化检测,结果均为：CD3ε⁺、粒酶B⁺及EBER1/2-ISH...     

喉鳞状细胞癌细胞核DNA定量及其与预后的...

DNA ploidy, S% and proliferative index (PI) of carcinomatous cell were evaluated with FCM in 41 patients with laryngeal primary squamous cell carcinomas which were operated on in our department during the period between Jan. 1979 to Oct. 1987. The results showed that DNA aneuploidy was identified in 65.85% of the cases (27/41) and patients with aneuploid carcinomas had higher S% (mean +/- standard: 23.22 +/- 9.57%), higher PI (mean +/- standard: 37.59 +/- 9.57%) and higher rate of relapse and/or metastasis than those with diploid tumours (55.55% vs 11.77%, P < 0.025). Patients with aneuploid carcinomas also correlated with an unfavorable prognosis compared with those with diploid tumors. Cases with aneuploid and diploid carcinomas had 66.11% and 92.86% respectively for two years postoperation survival rate; 46.74% and 92.86% respectively for five years postoperation survival rate (Log-rank test: chi 2 = 5.96, P < 0.025). We conclude that nuclear DNA measurement with flow cytometry is of important significance in estimating prognosis and guiding treatment for laryngeal squamous cell carcinomas.     

106例成人急性白血病流式细胞术免疫分型分析

OBJECTIVE: To study the immunophenotyping of adult patients with acute leukemia and its association with the prognosis. METHODS: Immunophenotyping was performed in 106 adult patients with acute leukemia by three-color flow cytometry analysis using CD34/SSC gating. RESULTS: The antigens expressed in 71 patients with acute myeloid leukemia (AML) were mainly CD13, CD33, HLA-DR, CD34 and CD117. Lymphoid antigen expression was identified in 23.9% adult AML patients and CD56 antigen expression in 15.5% of the AML patients. In 29 patients with acute lymphoblastic leukemia (ALL), the expressed antigens were mainly HLA-DR, CD10, CD19, CD34 and CD7, and 34.5% of these patients were found to be positive for myeloid antigen expression. Complete remission (CR) rate in AML patients with lymphoid antigen expression after chemotherapy was lower than that in AML patients without lymphoid antigen expression (52.9% vs 77.8%, P<0.05), and no significant impact was noted of myeloid antigen expression in ALL on the CR of the patients (70.0% vs 94.7%, P>0.05). The CR rate in AML with CD56 antigen expression was lower than that in AML without CD56 expression (36.4% vs 78.3%, P<0.025), and the CR rate in CD34+ AML was lower than that in CD34- AML (56.0% vs 80.4%, P<0.05). CONCLUSIONS: Gating of CD45/SSC can eliminate the interference of normal cells to render more reliable immunophenotyping results. The expressions of CD56+, CD34+, lymphoid antigen in adult AML patients, who have lower CR rate, often signify poor prognosis.     

Comparison of DNA flow cytometry and microspectrophotometry in gastric carcinoma

Flow cytometric (FCM) and microspectrophotometric (MSP) measurements of nuclear DNA content were made in 52 fresh gastric carcinoma specimens and in 30 gastric mucosal specimens from patients with chronic gastritis DNA aneuploidy was found in 60% (32/53) cases of gastric carcinoma, and it appeared more frequently in well-or moderately differentiated tubular adenocarcinoma (90%) than in undifferentiated and mucous cell carcinoma (23.6%) (P less than 0.01). No aneuploidy was found in benign gastric mucosal samples, but their proliferative cell fractions were significantly higher than those in normal control gastric mucosal samples (p less than 0.01). The comparison of the results measured with FCM and MSP showed a high correlation rate (r = 0.07, p less than 0.005). The advantages and limitations of both methods are discussed, and the authors think that the two can be regarded complementary to each other and may be used in combination for more precise cytochemical analysis.     

405例胎儿畸形的超声诊断与分析

本文总结10572例中、晚期孕妇产前B超检查资料,检出胎儿先天性畸形405例,其检出率为3.8％,并对胎儿畸形进行分析,其中神经管缺陷居首位,占86.91％,泌尿系统畸形占2.72％,消化系统畸形占1.23％,其它畸形占9.14％。结论:超声对胎儿畸形的早期诊断与分类,具有其它诊断不能比拟的优点。     

流式细胞技术检测细胞内雌激素受体方法的探讨

目的 建立一种灵敏度高、特异性强的细胞内雌激素受体检测方法。方法 通过加入不同的试剂浓度及改变细胞渗透剂等，观察细胞内雌激素受体的表达。结果 随加入抗休浓度的增加，细胞内雌激素受体表达增加，用0．5％，Saponin溶液穿透细胞膜较用0．1％Tritonx-100，雌激素受体水平明显为高（P＜0．01）。结论 在用流式细胞技术检测细胞内雌激素受体时，加入抗体的最佳浓度为45μg/ml；细胞渗透剂以用0．5％Saponin溶液为宜。     

CD42a定量检测血小板

OBJECTIVE: To investigate a reference method for platelet (PLT) count. METHODS: 76 samples of daily whole blood of high, normal and low values in patients were collected and analyzed by flow cytometry (FCM) with CD42a monoclonal antibody that could combine specifically with the platelet membrane glycoprotein Ib-Ix (GPIb-Ix). PLT counts were analyzed by multiplying PLT/RBC ratio from FCM with the RBC that was counted by a calibrated blood analyzer. Meanwhile, the results of PLT from FCM were compared with that from blood analyzers SE-9500, NE-1500, CD-1600 or manual count. RESULTS: The within-replicate coefficient of variance(CV) of FCM was less than 4% for a high count(501 x 10(9)/L) and middle count(184 x 10(9)/L), the CV of low count(23 x 10(9)/L) was 11.432%, the between-replicate CV was 10.792%, and the overall reproducibility was less than 5%. Linearity was demonstrated well(r = 0.9975 P < 0.001) when the PLT counts were 20 x 10(9)/L-700 x 10(9)/L. PLT count was stable at room temperature within 6 hr. The carry-over was 0.87%. The results between FCM and SE-9500, NE-1500, CD-1600 or manual count was comparable (r > 0.975, P < 0.001). CONCLUSION: These findings confirm that FCM for PLT count is specific, accurate and precise and could be considered as a candidate of reference method for PLT count.