Effects of Metallothionein on Isolated Rat Heart

To investigate the effects of metallothionein （MT） on isolated rat heart, 16 Wistar rats were randomly divided into 2 groups. In control group （group C）, distilled water was injected intraperitoneally and 24 h later isolated hearts were perfused with Langendorff and stored at 4℃ for 3 h with histidine-tryptophan-ketoglutarate （HTK） solutions, and then isolated hearts were perfused for 2 h by Langendorff. In experimental group （group E）, 3.6% ZnSO4 was injected intraperitoneally, 24 h later isolated hearts were perfused by Langendorff and stored at 4℃ for 3 h with HTK solutions, and then the isolated hearts were perfused for 2 h with Langendorff. MT content, the recovery of hemodynamics, myocardial water content （MWC）, lactate dehydrogenase （LDH） and creatine kinase （CK） leakage, adenosine triphosphate （ATP） and malondialdehyde （MDA） content, superoxide dismutase （SOD） activity, myocardial cell Ca^2＋ content, Ca^2＋-ATPase activity of mitochondria （[Ca^2＋-ATPase]m） and its Ca^2＋ content （[Ca^2＋]m）, synthesizing ATP activity of mitochondria （[ATP]m）, and the ultrastructure of cells were examined. There were a significant increase in group E in hemodynamic recovery, ATP content, SOD activity, [Ca^2＋-ATPase]m activity, [ATP]m activity, and substantial reduction in MWC, LDH and CK leakage, MDA content, myocardial cell Ca^2＋ content, [Ca^2＋]m content, and the ultrastructural injury were obviously milder than that of group C. This study demonstrated that MT has protective effects on isolated rat heart.     

Inhibitory Effects of Chlorpheniramine and Astemizolum on Contraction of Isolated Rat and Rabbit Uteri Induced by Oxytocin an

(陈锋)(侯淑贤)(代宗顺)ＩｎｈｉｂｉｔｏｒｙＥｆｆｅｃｔｓｏｆＣｈｌｏｒｐｈｅｎｉｒａｍｉｎｅａｎｄＡｓｔｅｍｉｚｏｌｕｍｏｎＣｏｎｔｒａｃｔｉｏｎｏｆＩｓｏｌａｔｅｄＲａｔａｎｄＲａｂｂｉｔＵｔｅｒｉＩｎｄｕｃｅｄｂｙＯｘｙｔｏｃｉｎａｎｄＰＧＦ_（?..     

Change of Hydroxyl Radical Content in Isolated Rat Heart Perfused with Adriamycin and the Influence of Cordyceps Sinensis Alc

本实验采用Ｌａｎｇｅｎｄｏｒｆ大鼠离体心脏灌流模型，以水杨酸为捕捉剂，利用其与阿霉素灌流时产生的ＯＨ·反应生成的羟苯甲酸（ＤＨＢＡ）为指标，用高效液相色谱法（ＨＰＬＣ）检测了阿霉素损伤时冠脉流出液和心肌组织中ＤＨＢＡ含量，初步探讨了阿霉素性心肌损伤和ＯＨ·的关系。同时用生化方法测定了冠脉流出液中乳酸脱氢酶（ＬＤＨ）含量，作为心肌损伤的参考指标。并且观察了冬虫夏草醇提取物（ＣＳＡＥ）预灌注时对ＯＨ·生成量和ＬＤＨ漏出量的影响。结果表明：１５ｍｇ／Ｌ阿霉素灌注时，冠脉流出液和心肌组织中ＯＨ·含量显著增加，损伤加重；而使用０．４ｇ／ＬＣＳＡＥ预灌时，ＯＨ·含量明显下降，损伤减轻（Ｐ均＜０．０１）。ＬＤＨ与冠脉流出液及心肌组织中ＯＨ·含量均呈正相关（γ分别为０．９２２和０．９４９）。提示：①阿霉素性心肌损伤和ＯＨ·的产生增多有关。②ＣＳＡＥ能拮抗ＯＨ·的产生或清除已产生的ＯＨ·，具有保护作用。     

Effects of Ligustrazine on the Contraction of Isolated Rabbit Corpus Cavernosum Strips

Ligustrazine(tetramethylpyrazine,TMP)is avasoactiveingredient that has beenisolated and pu-rified fromLigusticum Chuanxiong Hort.(Um-belliferae)behind an ancient Chinese herbal reme-dy.It has been widely used for the treat ment of avariety of vascular diseases such as occlusive cere-bral arteriolar diseases,notable ischemic strokeand pul monary hypertension secondary to chronicobstructive pul monary disease(COPD)due to itsvasodilatory actions.Ligustrazine has previouslybeen shown to be eff…     

Effects of Isoprenaline，Phenylephrine on Heart and Influence of Nifedipine on These Effects

ＥｆｆｅｃｔｓｏｆＩｓｏｐｒｅｎａｌｉｎｅ，ＰｈｅｎｙｌｅｐｈｒｉｎｅｏｎＨｅａｒｔａｎｄＩｎｆｌｕｅｎｃｅｏｆＮｉｆｅｄｉｐｉｎｅｏｎＴｈｅｓｅＥｆｆｅｃｔｓＭＡＹｅ－ｘｉｎ（马业新）；ＹＵＳｈｕ（余枢）；ＺＨＡＯＨｕａ－ｙｕｅ（赵华月）（Ｄｅｐａ...     

Effects of Lipoteichoic Acid induced Delayed Preconditioning on Ischemia-reperfusion Injury in Isolated Rat Hearts

Thereisgeneralagreementthatpreconditioning(PC)isthestrongestformofinvivoprotectiona gainstmyocardialischemicinjury Thesecondwin dowofprotection (SWOP)ordelayedPCwasde scribedbyYellonandBaxter[1] etalasenhancedtol erancetolethalischemia 2 4hafteraPCstimulusofbriefrepetitiveepisodesofischemia ItisreportedthatthedelayedPCappears 12to 2 4hlaterandlastsfor 3- 4days Inadditiontoreducingmyocardialin farctsize ,thedelayedPCenhancestheresistanceofmyocardiumagainstfurtherarrhythmias,endothelialdys…     

Modification of Platelet Activating Factor-induced Injury of Isolated Perfused Rat Lung by Leukocytes

Platelet activating factor(PAF)increased pulmonary vascular permeability and Induced lung edema. PAF(1.8 ng/ml)combined with leukocytes caused more serious lung injury than PAF was washed out after 10 min perfusion,its actions to increase fluid filtration coefficient, protein extravasation and lung weight gain remained but were less serious. These remaining actions could be inhibited by addition of 1.6×10~8 fresh leukocytes, of which 93.6％ remained in the perfusion     

Effects of High-Carbohydrate Diet on Plasma Insulin and cAMP Metabolism in Rat

Wistar rats that had been kept on a normaldiet for 1 week were put on a high-carbohydrate(80% of total calories) diet composed of starch,mainly in wheat flour, for 3 days. The fastingplasma insulin and triglyceride (TG) contents ofthese rats were higher than those of the normaldietary rats (P<0.01;P<0.01). After feeding thehigh-carbohydrate (CHO) diet for 6 days, the     

Effects of Lead on Systolic and Diastolic Cardiac Functions

lNTRODUCTlONNowadays,theeffectsofleadondigestivc,hcmatopoietic,andncrvoussystemsandonkidneyarewellknown,butthereislessknow1edgcofcardiotoxicityofIead.Illrecentyears,manysurveyswereconductedoncardiovasculareffectsoflead,butmostofthemwerecollcernedwiththerelationshipbetwcenbloodpressureandlcadburdenorwerebasedonanima1experiments.ThepurposcofthisstudywastoevaluatetheeffectsoflcadonhumansystolicanddiastoliccardiacfunctionsbyDopplerechocar-diographyandtodrawattentiontothemyocardialinvolvementil…     

Effects of Berberineon Cardiac Function and ATPase Activity of Postischemic Myocardial Stunned Rat Hearts

ＥｆｆｅｃｔｓｏｆＢｅｒｂｅｒｉｎｅｏｎＣａｒｄｉａｃＦｕｎｃｔｉｏｎａｎｄＡＴＰａｓｅＡｃｔｉｖｉｔｙｏｆＰｏｓｔｉｓｃｈｅｍｉｃＭｙｏｃａｒｄｉａｌＳｔｕｎｎｅｄＲａｔＨｅａｒｔｓＬｉＱｉｎｇｐｉｎｇ（李庆平）ＧｕｏＢｉｎｇｈｕｉ（郭炳辉）Ｒａ...     

灌注液中PCO_2升高对离体大鼠心脏收缩及舒张功能的影响

用改进的Langendorff法灌注离体大鼠冠状动脉,观察灌注液中PCO_2升高对离体大鼠心脏左室收缩及舒张功能的影响。结果表明:灌注液中PCO_2升高可直接引起左室收缩和舒张功能降低;还可通过降低pH值,使心脏的收缩和舒张功能降低。     

肌细胞特异性microRNAs对收缩舒张生物学效应调控机制的研究进展

 肌细胞特异性microRNAs (muscle-specific microRNAs,myomiRs)是一类特异性表达在肌组织中的内源性非编码小分子RNA,通过转录后水平负调控相关基因的表达,广泛参与到一系列生物学过程中,影响疾病的发生发展。肌细胞相关疾病(如慢性阻塞性肺炎、肥厚型心肌病等)的发生、发展可引起myomiRs及其下游靶基因表达改变,从而进一步影响疾病的发展、预后及转归。本文将综述miR-1、miR-133、miR-206、miR-208和miR-499等常见myomiRs在横纹肌和非横纹肌收缩舒张机制中的作用,重点关注myomiRs对肌细胞收缩舒张生物学效应的影响,以期为肌细胞相关疾病治疗提供新思路。     

糖尿病大鼠膀胱舒张及收缩功能实验研究

目的 阐明早期糖尿病膀胱舒张和收缩功能改变。方法 以链脲佐菌素诱导大鼠糖尿病。用蔗糖利尿模拟多尿性膀胱功能变化,对比糖尿病、蔗糖利尿和正常对照大鼠离体膀胱收缩、舒张变化及ｃＡＭＰ含量。结果 早期糖尿病鼠膀胱最大收缩力和持续收缩力下降,膀胱舒张能力也有下降,膀胱ｃＡＭＰ含量明显减少,结论 糖尿病早期膀胱逼尿肌收缩、舒张功能均有明显受损;同时,大量利尿可使膀胱容量和湿重增加,但不是糖尿病膀胱病变的主要     

氨力农对心脏瓣膜替换术后急性心功能不全患者血液动力学的影响

观察２４例心脏瓣膜替换术后急性心功能不全患者，静脉注射氨力农对血液动力学的影响。结果显示，注射后５ｍｉｎ心脏指数（ＣＩ）、每搏指数（ＳＩ）、左心作功指数（ＬＣＷＩ）和左心每搏作功指数（ＬＶＳＷＩ）均显著增高（Ｐ＜０．０５）；肺动脉楔压显著降低（Ｐ＜０．０５）；ＣＩ、ＳＩ、ＬＣＷＩ和ＬＶＳＷＩ于注射后６０ｍｉｎ增加最为明显（Ｐ＜０．０１），随后均维持在较稳定的水平；注射后３０ｍｉｎ体循环阻力指数、肺循环阻力指数和右房压亦显著降低（Ｐ＜０．０５）；心率、平均动脉压、平均肺动脉压和心率收缩压乘积，在用药期间均无显著改变；右心作功指数和右心每搏作功指数虽有升高的趋势，但无统计学意义。提示氨力农静脉注射后继以静脉滴注，在短时间内能有效地改善瓣膜替换术后急性心功能不全患者的血液动力学，而不增加心肌耗氧量。     

静注国产氨吡酮治疗心衰

随机、双盲法观察结果表明:在首剂静注国产氨吡酮1 mg/kg,并每天以7.5μg·kg~(-(?))/min 静滴6h 连续1周后,71.9%的心衰患者心功能改善,超声多普勒心指数由3.36±0.87增至3.88±1.03 L·min~(-1)/m~2.8例血液动力学检查示心指数上升23.8%,且与血药浓度相关,肺楔嵌压降低37.5%.氨吡酮和对照组室性早搏发生率分别为18.8%和9.1%.     

心脏停搏液预处理对大鼠离体心脏缺血性挛缩及再灌注冠脉流量的影响

目的　观察心脏停搏液（ 简称停搏液）预处理对大鼠离体心脏缺血性挛缩的发生及再灌注冠脉流量的影响。方法　选用ＳＤ大鼠离体Ｌａｎｇｅｎｄｏｒｆｆ 逆行灌注模型,观察应用停搏液时心脏缺血性挛缩的发生时间和幅度以及再灌注心脏冠脉流量的改变。结果　停搏液预处理显著增加了心脏缺血再灌注期间的冠脉流量,显著延迟了缺血期间心肌挛缩的发生时间,并降低其幅度。结论　停搏液预处理减轻缺血心肌挛缩的程度,可能是其心脏缺血再灌注损伤的保护作用的机制之一。     

蒺藜皂苷对大鼠离体心脏血液动力学的影响

目的：观察蒺藜皂苷（GSTT）对大鼠离体心脏血流动力学的影响。 方法：采用Langendorff离体心脏灌流方法,以动脉血压（AP）、左室收缩压（LVSP）、左室舒末压（LVEDP）、室内压最大上升速率（+dp/dt_max）、室内压最大下降速率（-dp/dt_max）及心率（HR）、主动脉流量（AF）、冠脉流量（CF）8项血流动力学参数为指标,观察GSTT对心肌收缩性能的直接影响。结果：给药后5~20 min,100、150和200 mg•L^-1 3个剂量组GSTT均可使AP、LVSP、±dp/dt_max、AF的绝对值降低,使 LVEDP、CF的绝对值升高,与对照组比较差异均有显著性（P<0.05或P<0.01 ）;GSTT 3个剂量组均可拮抗因细胞外液钙离子浓度增加所致的正性肌力作用,同时拮抗因细胞外液葡萄糖浓度降低所致的心肌收缩力减弱,与对照组比较差异均有显著性（P<0.05或P<0.01）。 结论：GSTT可使离体工作心脏产生剂量依赖性的负性肌力作用,同时增加正常离体大鼠心脏冠脉流量,其负性肌力作用可能与阻滞钙通道有关,且这种负性肌力对心肌缺糖具有保护作用。     

室性期前收缩不同负荷与心脏结构和心功能关系的研究

目的探讨室性期前收缩（早搏）不同负荷与患者心脏结构及功能之间的联系。方法104例室性早搏患者根据室早负荷值分为低负荷组、中负荷组和高负荷组。比较左心室射血分数（LVEF）、左心室收缩末期内径（LVESD）、左心室舒张末期内径（LVEDD）、左心房最大容积（LAVmax）和左心房最小容积（LAVmin）和血清氨基末端脑利钠肽前体（NT-proBNP）。结果低负荷、中负荷和高负荷组的NT-proBNP、LVEF、LVESD、LVEDD、LAVmax和LAVmin比较差异均具有统计学意义（P〈0．05）。负荷与LVEF呈直线负相关（r=-0．6,P〈0．01）,与LVESD（r=0．47,P〈0．01）、LVEDD（r=0．66,P〈0．01）、NT-proBNP（r=0．4,P〈0．01）、LAVmax（r=0．68,P〈0．05）和LAVmin（r=0．52,P〈0．05）呈正相关。结论室性早搏高负荷患者,左心室扩大,心功能下降。     

脂肪乳剂对布比卡因诱导的离体大鼠心脏停搏的复苏作用

目的：观察脂肪乳剂治疗布比卡因诱导的离体大鼠心脏停搏的复苏效应和对复苏后心脏功能的影响。方法：Sprague-Dawley成年雄性大鼠18只,随机分为实验组和对照组。建立Langendorff离体心脏灌注模型。以100μmol/L布比卡因诱导心脏停搏造模后实验组改用含30μmol/L布比卡因及2%脂肪乳剂的K-H液灌注心脏,对照组改用含30μmol/L布比卡因的K-H液灌注心脏。记录两组恢复窦性心律情况,记录停跳前基础值及恢复窦性心律后40 min内的心率、左心室发展压和心率-左心室发展压乘积（RPP）。结果：与对照组比较,实验组心脏恢复窦性心律时间显著缩短（P〈0.05）,心脏造模成功后3 min内恢复窦性心律的例数实验组显著多于对照组（P〈0.01）。恢复窦性心律后,实验组各时间点的心率、RPP均显著高于对照组（P〈0.05或0.01）。结论：脂肪乳剂对布比卡因诱导的离体大鼠心脏停搏治疗有效,能加速心脏复苏,改善复苏后心脏功能。     

超细颗粒物对大鼠离体灌注心脏功能的影响

目的: 研究超细颗粒物(ultrafine particulates,UFPs)对大鼠离体心脏功能的影响及其机制。方法: 以含或不含UFPs的台式液经Langendorff系统持续灌流大鼠离体心脏模型40 min,观察灌流前后两组大鼠心脏血流动力学指标[左心室舒张压(left ventricular developed pressure,LVDP)、左心室内压最大上升和最大下降速率(±dp/dt_max)及冠脉流量(coronary flow,CF)]的变化。收集肺动脉流出液,采用硫代巴比妥酸法测定丙二醛(malondialdehyde,MDA),水溶性四唑盐法测定超氧化物歧化酶(superoxide dismutase,SOD), 比色法测定总抗氧化能力(total antioxidant capacity,TAOC)。免疫组织化学法和Western blots法测定两组心脏标本p-p38 MAPK、p-JNKs、p-ERKs的表达。结果: 相对于对照组,UFPs灌流组大鼠离体心脏功能指标LVDP、+dp/dt_max、-dp/dt_max、CF分别从(82.6 ± 2.1) mmHg、(1 624 ± 113) mmHg/s、(1 565 ± 116) mmHg/s、(12.0 ± 0.2) mL/min降至灌注结束时的(56.8 ± 4.4) mmHg、(1 066 ± 177) mmHg/s、(1 082 ± 134) mmHg/s、(8.7 ± 0.3) mL/min,各指标灌流结束时相比灌流初始值差异均有统计学意义(P<0.05)。UFPs灌流组肺动脉流出液MDA含量明显高于对照组[(1.95±0.18) nmol/L vs. (0.98±0.14) nmol/L,P<0.05],而SOD、TAOC明显低于对照组[(6.50±1.04) U/mL vs. (12.50±1.87) U/mL,(3.67±0.82) U/mL vs. (6.83±1.16) U/mL, P<0.05 ]。UFPs灌流组p-p38 MAPK、p-JNKs、p-ERKs 较对照组表达明显增加(P<0.05)。结论: UFPs短期暴露对大鼠离体心脏有直接的急性毒性作用,其作用机制可能与氧化应激及MAPK信号通路激活有关。