三磷酸腺苷结合盒转运体成员2在人脑胶质瘤中的表达及其与神经巢蛋白表达的关系

Objective To study the expression of ATP-binding cassette superfamily G member 2 ( ABCG2) and its relationship with the malignant degree and expression of nestin in human gliomas. Methods Fifty-two gliomas of different WHO grades and 8 normal brain tissues were detected for the mRNA expression of ABCG2 by using real-time quantitive polymerase chain reaction (PCR). And the protein expression of ABCG2 and nestin was detected by immunohistochemistry. Results The mRNA of ABCG2 was overexpressed in gliomas as compared with that in normal brain tissues (P<0. 05), and up-regulated with the increase of pathologic degrees (P<0. 05 ). The positive protein expression rate of ABCG2 in 52 gliomas was 32.7%. The positive rate was significantly higher in grade Ⅲ-Ⅳ than in grade Ⅰ -Ⅱ (x2 =4. 62, P < 0. 05). And the expression level of ABCG2 was obviously related with the expression of nestin (x2= 7. 60,P < 0.05). Conclusion The expression level of ABCG2 was obviously related with glioma pathological grade and the expression of nestin. Brain tumor stem cells may have some homology with nerve stem cells.     

三磷酸腺苷结合盒转运体成员2在人脑胶质瘤中的表达及其与神经巢蛋白表达的关系

Objective To study the expression of ATP-binding cassette superfamily G member 2 ( ABCG2) and its relationship with the malignant degree and expression of nestin in human gliomas. Methods Fifty-two gliomas of different WHO grades and 8 normal brain tissues were detected for the mRNA expression of ABCG2 by using real-time quantitive polymerase chain reaction (PCR). And the protein expression of ABCG2 and nestin was detected by immunohistochemistry. Results The mRNA of ABCG2 was overexpressed in gliomas as compared with that in normal brain tissues (P<0. 05), and up-regulated with the increase of pathologic degrees (P<0. 05 ). The positive protein expression rate of ABCG2 in 52 gliomas was 32.7%. The positive rate was significantly higher in grade Ⅲ-Ⅳ than in grade Ⅰ -Ⅱ (x2 =4. 62, P < 0. 05). And the expression level of ABCG2 was obviously related with the expression of nestin (x2= 7. 60,P < 0.05). Conclusion The expression level of ABCG2 was obviously related with glioma pathological grade and the expression of nestin. Brain tumor stem cells may have some homology with nerve stem cells.     

瘦素和瘦素受体在食管癌中的表达及其意义

Objective To investigate the role of leptin and leptin receptor in carcinogensis and development of esophageal carcinoma. Methods The expression of leptin and leptin receptor was detected in 52 cases of esophageal carcinoma tissues and 49 cases of normal esophageal tissues by immunohistochemistry. The correlation between their expression and clinicopathological parameters was also analysized. Results The expression rate of leptin and leptin receptor in esophageal carcinoma was 78. 8% (41/52) and 82.7% (43/52) respectively, and the rate in normal esophagus was 58.3% (28/49) and 59.2% (29/49) respectively. The expression rate of leptin and leptin receptor both had statistically significantly differences between esophageal carcinoma and normal esophagus tissues (P ＜ 0. 05). The expression rate of leptin was associated with position, tumor size, differentiation, lymphatic metastasis and TNM stage (P ＜ 0. 05 ). Conclusion Leptin and leptin receptor were dually expressed in esophageal carcinoma.They played important roles in the process of carcinogensis and development of esophageal carcinoma.     

肌动蛋白交联蛋白与血管内皮生长因子在肾细胞癌中的表达及其相关性

Objective To investigate the expression of Fascin and vascular endothelial growth factor (VEGF) in renal cell carcinoma (RCC) and the correlation with the biological behaviors. Methods The immunohistochemistry staining method was used to detect the expression of Fascin and VEGF in 92 cases of RCC and 20 cases of normal renal tissues as controls. Results The expression of Fascin in carcinoma tissue was significantly higher than that in normal tissues ( P ＜ 0. 05 ). Positive expression of Fascin and VEGF in renal cell carcinoma tissue was correlated with tumor grade ( P ＜ 0. 05 ) and clinical stage (P ＜0. 05 ) , but not with age, gender and different histological categories (P ＞ 0. 05 ). There was also a positive correlation between Fascin and VEGF (P ＜ 0. 05 ). Conclusion Fascin and VEGF are objective markers to estimate the behaviors of renal cell carcinoma.     

胃癌组织中黏附分子整合素CD11c的表达及其与预后的关系

目的 探讨黏附分子家族的成员CD11c在胃癌组织中的表达及其与预后的关系.方法 采用免疫组织化学染色法检测125例胃癌患者癌组织CD11c的表达.结果　CD11c在胃癌中的表达高于胃炎和胃息肉组织.CD11c与患者性别、年龄、病理分级、胃癌部位无关(P＞0.05);与肿瘤大小、浸润深度、淋巴结转移、组织类型、复发和TNM分期明显相关.肿瘤直径＜5 cm组CD11c的表达水平(12.4±6.8)高于肿瘤直径≥5 cm组(7.7±4.6),差异有统计学意义(t=3.98,P＜0.01).浸润深度与CD11c表达水平显著关联,未侵入肌层组胃癌的CD11c表达水平(15.4±8.5)显著高于侵入肌层组(9.5±5.6),差异有统计学意义(t=3.1,P＜0.05).淋巴结转移与CD11c表达水平显著关联,未有淋巴结转移的CD11c表达水平(15.3±6.6)显著高于淋巴结转移组(8.6±5.1),差异有统计学意义(t=5.44,P＜0.01).未复发组的CD11c表达水平(12.3±7.4)高于复发组(9.0±5.3),差异有统计学意义(t=2.90,P＜0.01).分化型组的CD11c表达水平(9.3±5.3)低于低分化型组(12.6±7.8),差异有统计学意义(t=2.68,P＜0.01).CD11c表达随肿瘤临床分期的递增而降低,各组间差异有统计学意义(P＜0.01).多因素COX模型分析,在调整了性别、年龄、肿瘤大小、是否侵及深肌层、分化程度后,与CD11c细胞低表达组比较,高表达组有降低胃癌死亡风险的趋势(RR=0.51,95%CI=0.22～1.16).结论　CD11c表达与胃癌预后有关,可以作为反映患者免疫状态和预后的指标之一.

Abstract：

Objectiye To investigate the expression of CD11c in gastric carcinoma and the clinical significance. Methods The expression of CD11 c in gastric carcinoma, gastritis tissue and gastric polyp was detected by using immunohistochemical assay. Results The expression level of CD11c in gastric carcinoma was higher than that in gastritis tissue and gastric polyp. There was no significant difference in the CD11 c expression among gender, age, pathological grade, primary area (P ＞ 0. 05 ). There was significant difference in the CD11c expression among tumor size, invasive depth, lymph node metastasis, histological type, recurrence and clinical stage. The expression of CD11c in tumor size ＜5 cm group ( 12.4 ±6. 8 ) was higher than that in tumor size ≥ 5 cm group (7.7 ± 4. 6), ( t = 3.98, P ＜ 0. 01 ). The expression of CD11c in non-invasion group ( 15.4 ± 8.5 ) was obviously higher than that in invasion group (9.5 ±5.6), ( t = 3.1 ,P ＜ 0. 05 ). The expression of CD11c in non-lymph node metastasis group ( 15.3 ± 6. 6 )was obviously higher than that in lymph node metastasis group ( 8. 6 ± 5. 1 ), ( t = 5. 44, P ＜ 0. 01 ). The expression of CD11c in non-recurrence group ( 12.3 ±7.4) was higher than that in recurrence group (9. 0± 5.3), ( t= 2.90, P ＜ 0. 01 ). The expression of CD11 c in differentiation group ( 9. 3 ± 5.3 ) was lower than that in low differentiation group ( 12. 6 ± 7. 8 ), ( t = 2. 68, P ＜ 0. 01 ). There was significant diference among groups of clinical stage (P ＜0. 01 ). The multivariate Cox analysis revealed that the risk of death in high expression group was significantly lower than that in low expression group (RR =0. 51, 95% CI=0. 22-1.16). Conclusion Detection of the CD11c expression in gastric carcinoma is beneficial to the judgment of the prognosis of gastric carcinoma and the choice of individualized treatment.     

表观沉默蛋白SUZ12与胃癌病理特征及预后的关系

目的 探讨表观沉默蛋白SUZ12在胃癌组织中的表达及其与胃癌患者临床病理指标和预后的关系.方法 采用免疫组化染色方法检测SUZ12蛋白在97例胃癌组织中的表达,分析其与胃癌患者临床病理学指标及生存的关系.结果 SUZ12蛋白在本组胃癌组织中的阳性率为43%,明显高于临近非癌组织的15%(P=0.002).SUZ12基因表达与胃癌的分化程度(P=0.018)、淋巴结转移(P=0.023)、TNM分期密切相关(P=0.014).SUZ12表达阳性患者的预后明显比阴性患者差(P=0.024),为独立预后因素.结论 SUZ12基因可能在胃癌发生发展中起促进作用,其过表达程度可以作为胃癌某些生物学行为及判断预后的新的参考指标.

Abstract：

Objective To investigate the relationship between the expression of suppressor of zeste 12(SUZ12) and the clinicopathological parameters and prognosis in patients with gastric cancer. Methods SUZ12 protein expression levels in 97 cases of resected gastric cancer were detected by immunohitochemistry method, the relations between SUZ12 expression levels and the survival were estimated by Kaplan-Meier curve. Results The positive rate of SUZ12 expression in gastric cancer tissues was 43%, significantly higher than that (15%)in the adjacent noncancerous tissues( P = 0. 002). SUZ12-positive expression was significantly correlated with tumor differentiation ( P = 0. 018 ), lymph nodes metastasis ( P = 0. 023 ) and TNM staging(P = 0. 014). Gastric cancer patients with SUZ1 2-positive expression had worse prognosis than those with SUZ12-negative expression ( P = 0. 024). Conclusions SUZ12 is overexpressed in tissues of gastric carcinoma, SUZ12 is an independent prognosis factor of patients with gastric carcinoma.     

SEMA3F蛋白在肝细胞癌中的表达及其预后价值

目的 研究SEMA3F蛋白在肝细胞癌(hepatocelluar carcinoma,HCC)中的表达及其预后价值.方法 采用Western Blotting法检测32例HCC及相应癌旁肝组织标本中SEMA3F蛋白的表达.运用免疫组织化学法检测38例HCC以及其相应癌旁组织中的SEMA3F蛋白的表达和微血管密度(microvassel dainty,MVD)值,分析SEMA3F蛋白表达水平与MVD的相关性.结果 SEMA3F蛋白在32例HCC组织中的表达明显低于相应的PCLT(447.78±48.26比618.93±61.23,P＜0.05).SEMA3F在HCC组织中的表达水平与肿瘤的结节数目及是否具有包膜结构密切相关(P＜0.05).SEMA3F低表达组的复发转移率显著高于高表达组(P＜0.01)而其术后生存率则显著低于高表达组(P＜0.01).SEMA3F高表达组的MVD值明显低于低表达组(86.56±17.94比115.6±30.38,P＜0.01).结论 SEMA3F在HCC组织中表达下调与肝癌复发转移率高和预后差密切相关,提示其可能是一个潜在的HCC预后分子标志物.

Abstract：

Objective To investigate the expression of Semaphorin 3F (SEMA3F) protein in hepatocellular carcinoma (HCC) and to demonstrate its relationship with clinicopathological features and prognosis of HCC. Methods Western Blotting was carried out in 32 hepatocellular carcinoma samples and matched perineoplastic tissues to detect the expression of SEMA3F protein. The relationship between SEMA3F protein expression and clinicopathological features as well as prognosis of HCC patients was analyzed. Immunohistochemistry was used to show the location of SEMA3F in HCC cells and its relationship with microvessel density (MVD). Results The expression of SEMA3F protein in HCC tissues was significantly higher than in the perineoplastic tissues (447.78± 48.26 vs 618.93 ±61.23, P＜0. 05) and it was correlated closely with tumor capsulation and tumor nodular number (P＜0.05). Based on the Western Blotting and clinical follow-up data, we found that the survival time of HCC patients with a higher SEMA3F expression level was longer than those with a lower level, and the recurrent/metastatic time of HCC patients was significantly different between these two groups (P＜0.01). Immunohistochemistry demonstrated that SEMA3F protein localized in the cytoplasm of HCC cells and its expression correlated with HCC MVD. MVD in the low-level group was higher than the high-level group (115.6±30.38 vs 86. 56±17.94, P＜0.01). Conclusions SEMA3F expression in HCC was significantly down-regulated and correlated closely with tumor-capsulation, nodular number, and MVD, implicating SEMA3F may play an important role in recurrence and metastasis of HCC. It can be regarded as a prognostic marker in HCC patients.     

整合素β3与Fas蛋白在胃癌组织中的表达及其意义

目的 探讨整合素β3(Integin-β3)和Fas蛋白在胃癌组织中的表达、相互关系及意义.方法 应用免疫组织化学方法 检测48例胃癌组织及12例正常胃黏膜中Integrin-β3和Fas蛋白的表达.结果 胃癌组织中Integrin-β3阳性率显著高于正常胃黏膜(72.9%比25.0%,P＜0.01);正常胃黏膜中Fas蛋白阳性率显著高于胃癌组织(83.3%比33.3%,P＜0.01);Integin-β3阳性率与淋巴结转移、TNM分期显著相关(P＜0.01),而与肿瘤细胞分化程度无相关性(P＞0.05);Fas蛋白阳性率与淋巴结转移、TNM分期及肿瘤细胞分化程度均无相关性(P均＞0.05).胃癌组织中Integrin-β3与Fas表达具有显著等级负相关(r=-0.429,P＜0.01).结论 htegrin-β3的表达与胃癌细胞的浸润与转移密切相关.     

细胞分裂周期蛋白42mRNA和蛋白在食管鳞癌中的表达及其病理生物学意义

目的 探讨细胞分裂周期蛋白42(Cdc42)在食管鳞状细胞癌(ESCC)中的表达及其与临床病理参数间的关系.方法 应用实时荧光定量-聚合酶链反应(qRT-PCR)、蛋白免疫印迹和免疫组织化学方法,从mRNA和蛋白两个水平检测ESCC中Cdc42的表达,并分析其与临床病理参数的关系.结果 22对新鲜ESCC与癌旁正常组织中,Cdc42 mRNA在ESCC中的表达量(0.21±0.14)显著高于其在癌旁正常组织中(0.16±0.12)的表达(P＜0.05);Cdc42蛋白在ESCC中的表达量(0.83±0.35)高于其在癌旁正常组织中(0.75±0.24)的表达;在175对ESCC中,Cdc42蛋白的阳性表达率为73.7%(129/175),高于其在配对的癌旁正常组织中的表达62.9%(110/175,P＜0.05).此外,Cdc42的表达与ESCC患者的年龄、淋巴结转移及分化程度明显相关(P＜0.05).结论 Cdc42可能参与ESCC发生及转移的过程.

Abstract：

Objective To explore the expression of cell division cycle 42 (Cdc42) in human esophageal squamous cell carcinoma (ESCC) and investigate the association between Cdc42 and clinicopathological parameters. Methods The expression levels of Cdc42 mRNA and protein in ESCC and corresponding adjacent normal tissues were detected by real-time fluorescent quantitative polymerase chain reaction ( qRT-PCR), Western blotting and immunohistochemistry, respectively. The correlations between Cdc42 expression and clinicopathological parameters were analyzed. Results The expression of Cdc42 mRNA was significantly higher in ESCC tissues (0. 21 ± 0. 14 ) than that in corresponding controls (0. 16 ±0. 12) (t test,P ＜0. 05). The protein expression of Cdc42 was significantly higher in ESCC tissues (0. 83 ± 0. 35 ) than that in corresponding controls ( 0. 75 ± 0. 24). Immunohistochemistry revealed that 73.7% (129/175) of the ESCC samples had higher expression of Cdc42 protein than the corresponding controls[62. 9% (110/175) (χ2 test, P ＜ 0. 05 )]. Cdc42 expression level was correlated with age,lymphoid node metastasis and differentiation ( P all ＜ 0. 05 ), but not with the clinicopathological features,such as gender, ethnicity and macroscopical types (P ＞ 0. 05 ). Conclusion The higher expression of Cdc42 played a certain role in the carcinogenesis and metastasis of ESCC.     

Ezrin在胃癌组织中的表达及其临床意义

目的 探讨Ezrin在胃癌发生及发展过程中的作用.方法 收集2008年6月至2009年5月第三军医大学西南医院全军普通外科中心收治的60例胃癌患者的胃癌组织及正常胃黏膜组织标本,分别采用RT-PCR和Western blot法检测标本中Ezrin mRNA及蛋白的表达情况,分析Ezrin与患者性别、年龄、肿瘤分化程度、病理分期、浸润深度及淋巴结转移间的关系.采用t检验、x2检验、Spearman等级相关检验进行统计学分析.结果 60例正常胃黏膜组织中有33例(55％) Ezrin mRNA表达水平升高,45例(75％)Ezrin蛋白表达水平升高;60例胃癌组织中有21例(35％) Ezrin mRNA表达水平升高,22例(37％) Ezrin蛋白表达水平升高.Ezrin mRNA及蛋白在正常胃黏膜组织中的表达水平分别为1.30±0.04和3.57±0.45,在胃癌组织中的表达水平分别为0.53±0.36和0.96±0.18,两者比较,差异有统计学意义(t=5.309,22.617,P＜0.05).胃癌组织中Ezrin mRNA及蛋白表达水平呈正相关(r=0.602,P＜0.05).Ezrin mRNA和蛋白表达在胃癌不同病理分期、不同浸润深度、有无淋巴结转移方面差异有统计学意义(x2=6.41,6.49,4.62;5.40,8.87,4.12,P＜0.05),而在性别、年龄和分化程度方面差异无统计学意义(x2=0.50,0.07,1.07;0.01,1.16,1.96,P＞0.05).结论 胃癌组织中Ezrin表达水平降低,这可能是胃癌的发生、发展及转移的机制之一.     

STAT3及其磷酸化在食管鳞癌组织中的表达及其意义

目的 检测食管鳞癌组织中信号转导子和转录激活子3( STAT3)在mRNA、蛋白质和蛋白质磷酸化3种水平的表达,探讨其在食管鳞癌发生、发展、浸润、转移中的作用。方法 检测43例食管鳞癌组织中的STAT3 mRNA、STAT3和磷酸化STAT3( pSTAT3)的表达,并与相应癌旁正常食管组织作对照研究,分析STAT3 mRNA、STAT3和pSTAT3的表达与临床病理参数的关系。结果 43例实验样本中(1)食管鳞癌组织中STAT3 mRNA相对表达强度比值(1.43±0.59)较癌旁组织的比值(0.98±0.47)明显增高(P＜0.05);(2)食管鳞癌组织中STAT3、pSTAT3表达(2.16±0.39、1.40±0.15)也都显著高于癌旁组织(1.87±0.29、1.25±0.13,P＜0.05);(3)食管鳞癌组织中STAT3mRNA、STAT3和pSTAT3在肿瘤不同分化级别中表达差异有统计学意义,分化级别越低,表达水平越高(P＜0.05),并与肿瘤分化级别呈负相关(-1 ＜r＜-0.301,P＜0.05);它们在TNM分期中Ⅲ期组的表达均高于Ⅰ～Ⅱ期组(P＜0.05),伴有淋巴结转移组表达也都高于无淋巴结转移组(P＜0.05),并与两者呈正相关(两者均为0.301 ＜r＜1,P＜0.05);但未发现它们在性别、年龄、家族史、吸烟史中差异有统计学意义(P＞0.05)。结论 食管鳞癌组织中STAT3磷酸化异常激活后,导致STAT3mRNA、STAT3和pSTAT3的高表达,与食管鳞癌的分化、浸润、转移相关。     

人RUNT相关转录因子3、细胞周期素在胰腺癌组织中mRNA水平的表达及其与临床病理因素的关系

目的 探讨人RUNT相关转录因子3(RUNX3)、细胞周期素(Cyclin D1)基因在胰腺癌组织和癌旁组织中mRNA表达水平与临床意义.方法 采用逆转录-聚合酶链反应(RT-PCR)检测RUNX3、Cyclin D1基因在42例胰腺癌组织及其癌旁组织中mRNA表达水平,并分析RUNX3、Cyclin D1基因mRNA表达与临床病理因素的关系.结果 (1)RUNX3基因在42例胰腺癌组织中mRNA表达量相对值为:0.2469±0.0708;相应癌旁组织中相对值为:0.7091±0.1764,两者差异有统计学意义(t=15.7036,P＜0.01).(2) RUNX3基因mRNA表达水平在分化程度、淋巴结转移、临床分期等病理因素的差异有统计学意义(P＜0.05),而与性别、年龄等因素无明显相关(P＞0.05).(3) Cyclin D1基因在42例胰腺癌组织中mRNA表达量相对值为:0.7769±0.1456;相应癌旁组织中相对值为:0.2860±0.1224,两者差异有统计学意义(t=18.9158,P＜0.01).(4) Cyclin D1基因mRNA表达水平在分化程度、淋巴结转移、临床分期等病理因素的差异有统计学意义(P＜0.05),而与性别、年龄等因素无明显相关(P＞0.05).(5)胰腺癌中RUNX3基因的mRNA表达抑制与Cyclin D1基因的过表达呈明显相关(r=-0.320,P＜0.05).结论 RUNX3基因在胰腺癌组织中mRNA 表达抑制,Cyclin D1基因在胰腺癌组织中mRNA过表达,RUNX3表达抑制或缺失及Cyclin D1过表达与胰腺癌的发生、发展有关.     

食管鳞癌与细胞因子信号转导负调控因子3及其DNA甲基化的关系

目的 检测食管鳞癌(ESCC)组织中细胞因子信号转导负调控因子3(SOCS3)的DNA甲基化、mRNA及蛋白表达水平,探讨其在食管鳞癌发生、发展、浸润和转移中的作用.方法 采用甲基化特异性聚合酶链反应(MSP)、Real-Time聚合酶链反应(PCR)和Western blot法分别检测43例食管鳞癌组织中SOCS3的DNA甲基化、mRNA和蛋白表达水平,并与相应的癌旁正常食管组织进行对照研究,分析其与临床病理参数的关系.结果 (1)食管鳞癌组织SOCS3 DNA甲基化的阳性率(79.1%)明显高于癌旁组织(14.0%,P＜0.01);(2)食管鳞癌组织SOCS3 mRNA相对表达强度比值(0.53±0.30)明显低于癌旁组织(1.15±0.44,P＜0.01),食管鳞癌组织中甲基化组的SOCS3 mRNA表达(0.45±0.24)显著低于非甲基化组(0.86±0.29,P＜0.05);(3)食管鳞癌组织SOCS3蛋白表达(1.66±0.22)显著低于癌旁组织(1.83±0.15,P＜0.01),食管鳞癌组织中甲基化组SOCS3蛋白表达(1.61±0.21)显著低于非甲基化组(1.87±0.15,P＜0.01);(4)在TNM分期中Ⅲ期组表达均低于Ⅰ～Ⅱ期组(P＜0.05),伴有淋巴结转移组表达也都低于无淋巴结转移组(P＜0.05),未发现其在性别、年龄、家族史、吸烟史中有明显差异(P＞0.05);(5)食管鳞癌组织中SOCS3mRNA表达及其蛋白表达水平与肿瘤分化级别呈正相关(0.301＜r＜1,P＜0.05),与TNM分期、淋巴结转移呈负相关(-1＜r＜-0.301,P＜0.05).结论 食管鳞癌组织中SOCS3 DNA甲基化阳性率高,导致SOCS3基因表达下调,与食管鳞癌的分化、浸润和转移密切相关.     

胃癌中硒结合蛋白-1的表达及其临床病理学意义

目的 检测硒结合蛋白1(SBP1)在胃癌细胞系SGC7901、BGC823、正常胃黏膜上皮细胞系GES-1、胃癌组织以及正常胃黏膜组织中的表达水平,分析其表达变化与胃癌临床病理因素之间的联系,探讨其作为胃癌标记物的可能性.方法 回顾性分析2006年至2007年武汉大学中南医院收治的135例胃癌患者的临床资料,利用免疫组织化学染色法对胃癌组织及16例正常胃黏膜组织中SBP1蛋白表达部位及水平进行测定,并进行半定量评分.Western blot和RT-PCR检测细胞系SGC7901、BGC823、GES-1中SBP1蛋白和mRNA的表达水平.组间比较采用单因素方差分析,SBP1表达强度与临床病理因素的关系采用x2检验.结果 SBP1 mRNA在胃癌细胞系BGC823、SGC7901中的表达分别为0.120±0.020、0.133±0.015,显著低于其在正常胃黏膜上皮细胞系GES-1中的表达(0.907±0.015)(F=2106.462,P＜0.05).胃癌细胞系BGC823、SGC7901中的SBP1蛋白表达分别为0.253±0.015、0.273±0.015,显著低于其在正常胃黏膜上皮细胞系GES-1中的表达水平(0.877±0.025)(F=1026.758,P＜0.05).3例胃癌组织中SBP1呈强免疫反应,而16例正常胃黏膜中SBP1呈强免疫反应.SBP1蛋白表达减少与胃癌患者临床分期相关(x2=12.629,P＜0.05),而与患者的性别、年龄、肿瘤组织学分型、分化程度、浸润深度以及淋巴结转移无关(x2=2.142,0.860,1.838,5.001,4.858,1.994,P＞0.05).结论 SBP1表达减少可以作为一种胃癌诊断的新指标.SBP1下调在胃癌发生及进展中可能发挥着重要作用.