Regeneration of neuronal nitric oxide synthase (nNOS)-containing nerve fibers in rat corpus cavernosum

Aim: To investigate the effect of cavernous nerve injury on the nNOS-containing nerve fibers in rat corpus cavernosum.Methods: Thirty-three male SD rats were randomized into 3 groups: 5 rats underwent pelvic exploration without tran-section of cavernous nerve as the sham-operated controls, the unilateral injury group (14 rats) had the cavernous nerve cuton one side, and the bilateral injury group (14 rats) had the nerves cut on both sides. Corpora cavernosa were harvestedat the 3rd week and 6th month after surgery, nNOS-positive nerve fibers were examined with strepavidin peroxidase im-munohistochemistry techniques (SP method). Results: After bilateral ablation, the nNOS-positive nerve fibers weresignificantly decreased at both the 3rd week ( 17 ± 4) and the 6th month (16 ± 4). For the unilateral injury group, thenNOS-positive nerve fibers were similarly decreased on the side of the neurotomy at the 3rd week (18 ± 6), but by the 6thmonth, the number increased significantly (61±9) and approximated th     

Effect of diabetes and insulin treatment on nitric oxide synthase content in rat corpus cavemosum

Aim: To study the effect of diabetes mellitus and insulin treatment on rat penile nitric oxide synthase content.Methods: Male Wistar rats were divided at random into two groups: the Control (n = 8) and the Diabetic (n =17). Diabetes mellitus was induced by intraperitoneal injection of streptozotocin. The diabetic animals were then ran-domly divided into two subgroups; diabetic rats without insulin treatment (n = 7) and diabetic rats with insulin treat-ment (n = 10). The neuronal nitric oxide synthase (nNOS) in the penile corpus cavernosum were assayed by immumo-histochemical staining with specific antibody to nNOS and the nNOS-positive nerve fibers were counted semiquantita-tively under a high power microscope. Results: The nNOS- positive nerve fibres in diabetic rats with treatment washigher than that in diabetic rats without treatment ( P < 0.05) and lower than that in the controls ( P < 0.01). ThenNOS-positive nerve fibres in diabetic rat without treatment were also lower than that in the controls (     

神经生长因子基因修饰的骨髓间充质干细胞在糖尿病大鼠膀胱中的生长及表达

目的 将转入神经生长因子(NGF)的大鼠骨髓间充质干细胞(MSC)移植于糖尿病大鼠膀胱平滑肌组织内,观察MSC在膀胱组织内的存活和NGF基因的表达情况.方法 糖尿病组(30只)大鼠按链脲佐菌素(STZ)60 mg/kg行单次腹腔注射,对照组(15只)腹腔注射等体积的柠檬酸缓冲液.实验分组:对照组(正常大鼠膀胱)、发病组(糖尿病大鼠膀胱)、治疗组(糖尿病大鼠膀胱内移植转染NGF基因的MSC).溴苷法示踪NGF基因修饰的MSC在大鼠膀胱内的存活情况;RTPCR、ELISA法检测NGF基因在糖尿病大鼠膀胱内的表达情况.结果 单次腹腔注射STZ造模成功,8周后血糖仍处高位.NGF基因修饰的大鼠MSC移植入糖尿病大鼠膀胱内4周仍存活.ELISA检测结果显示对照组、发病组、治疗组大鼠膀胱NGF蛋白含量分别为(114±3)、(70±2)、(110±2)pg/ml,RT-PCR检测mRNA表达量分别为0.183±0.004、0.032±0.139、0.130±0.165.发病组与对照组相比NGF表达下降(P＜0.05),治疗组与发病组相比NGF表达上升(P＜0.05).结论转入NGF基因的大鼠MSC能在糖尿病大鼠膀胱中存活并稳定表达.

Abstract：

Objective To confirm that rat bone marrow mesenchymal stem cells (MSC) transfected with nerve growth factor (NGF) gene in the bladder tissue of diabetic rats bladder tissues can survive and stably express NGF. Methods A diabetic rat model was constructed. The BrdU-labelled MSC transfected with NGF gene were transplanted into the diabetic rats bladder tissues. BrdUlabelled immunohistochemistry was used to observe the growth of MSC transfected with NGF gene in the diabetic rats bladder tissues. The expression of NGF mRNA and protein were checked by RT-PCR and ELISA. Results A diabetic rat model was successfully built by a single intraperitoneal injectionof STZ. The blood glucose was still high after 8 weeks. NGF gene modified MSC could be detected in the bladder of diabetic rats by BrdU-labelled immunohistochemistry. The concentration of NGF in the control group, disease group and treatment group were ( 114 ± 3), ( 70 ± 2), ( 110 ± 2) pg/ml by ELISA and mRNA quantity by RT-PCR were 0. 183±0. 004, 0. 032±0. 139, 0. 130±0. 165, respectively. Compared with the control group, the expression of NGF gene was decreased (P＜0. 05) in the incidence group. The expression of NGF gene was increased (P＜0. 05) in the treatment group compared with the disease group. Conclusions The NGF gene-modified MSC could survive in diabetic rats bladder tissues. The NGF gene in MSC could stably express in diabetic rats bladder tissues.     

慢性压迫性脊髓损伤后大鼠运动功能及周围神经和骨骼肌中胰岛素样生长因子-1表达的变化

目的 观察慢性压迫性脊髓损伤后大鼠运动功能变化及周围神经和骨骼肌中胰岛素样生长因子-1(IGF-1)表达变化.方法 将50只Wistar雌性大鼠随机分为正常组、假手术组和慢性压迫组.慢性压迫组置入平头塑料螺钉对大鼠脊髓进行后路渐进性压迫,于2个月后分别压迫至20%、40%、60%左右程度.行神经功能观察;处死大鼠后取腓肠肌作为标本,分别进行IGF-1的免疫组织化学和原位杂交染色.结果 大鼠后肢瘫痪程度随压迫程度加重而加重,各组坐骨神经和骨骼肌中IGF-1蛋白和mRNA表达分别为:20%组(236.9±3.2)、(231.5±2.9)、(245.6±3.4)、(246.6±2.7);40%组(205.3±2.7)、(202.2±3.4)、(209.4±2.6)、(214.6±2.5);60%组(215.4±3.5)、(219.3±4.1)、(231.9±2.3)、(238.5±2.7).各压迫组坐骨神经及骨骼肌中IGF-1 mRNA和蛋白表达增加,与正常组比较差异有统计学意义(P<0.05).结论脊髓压迫性损伤可引起周围神经及骨骼肌中IGF-1表达增加,提示机体调动保护因素以减轻脊髓损伤并促进其再生.

Abstract：

Objective To observe the changes in motor function and expression of insulin-like growth factor-1 (IGF-1) of peripheral nerve and skeletal muscle in rats after chronic spinal cord compression. Methods A total of 50 Wistar rats were randomly divided into normal group (n=10), sham operation group (group A,n=10) and chronic compressive group (goup B,n=30). The rats in group B were given gradual compression on the posterior spinal cord using blunt plastics screw. Compression degree reached 20% (n=10), 40% (n=10) and 60% (n=10) respectively after two months. The rats were killed, and gastrocnemius muscle cells were removed. The expression levels of IGF-1 protein and mRNA in peripheral nerve and skeletal muscle were detected by immunohistochemistry and hybridization respectively after chronic compressive spinal cord injury. Results The rat hind limb paralysis was exacerbated with the increase of the compression. In the sciatic nerve and skeletal muscle, the expression levels of IGF-1 protein and mRNA were: (236.9±3.2), (231.5±2.9), (245.6±3.4), (246.6±2.7) in 20% group; (205.3±2.7), (202.2±3.4), (209.4±2.6), (214.6±2.5) in 40% group; (215.4±3.5), (219.3±4.1), (231.9±2.3), (238.5±2.7) in 60% group. The expression levels of IGF-1 protein and mRNA in peripheral nerve and skeletal muscle were significantly up-regulated after compression (P<0.05). Conclusion The results indicate that body transfers the protective factor to relieve injury of CNS.     

FK506及神经干细胞对异体神经移植生长的作用

目的 探讨大鼠坐骨神经异体神经移植后联合应用FK506(他克莫司)及神经干细胞(NSCs)的可行性.方法 制造大鼠坐骨神经缺损模型,异体神经移植修复;根据分组不施加干预因素、吻合口注入NSCs、腹腔注射FK506,通过运动及感觉评分、电生理检测、组织学检查,以及免疫组织化学染色,观察神经再生差异.结果 同时应用FK506及NSCs组,感觉及运动评分(5.32±1.43),神经传导速度(20.52±1.45)m/s,GAP43染色(965.72±369.17)都明显优于其他组(P＜0.05).结论 FK506结合NSCs对异体神经移植生长具有促进作用.

Abstract：

Objective This experiment study the effect of FK506 and neural stem cells on the regeneration in nerve allografts. Methods An established rat sciatic nerve model was used. Sciatic nerve ( 10 mm) deficits were created in the Lewis rats. Nerves allografts were harvested from BN rats to repair the deficits of the Lewis rats. Group A received no treatment. Group B received neural stem cells. Group D received FK506. Group C receive both neural stem cells and FK506. Nerve regeneration was evaluated by standardized pin-prick and toe-spread tests. Nerve samples and gastrocnemius muscles were harvested for examination. Muscle denervation atrophy was evaluated by gastrocnemius weights. GAP-43 and BrdU (Two-step immunohistochemistry detection reagent) were assayed by immunohistochemistry test. Results Improved functional outcomes were observed in group C when compared with other groups. Nerve conduction velocity were (20. 52 + 1.45) m/s. The score of sense and motion were higher ( 5.32 ± 1.43 ) than other groups. Improved histomorphology and electrophysiologiy were observed in group C when compared with other groups. The concentrations of GAP-43 (965.72 + 369. 17) were largest in group C, showing ststistically significant difference between groups (P ＜ 0. 05). Conclusion The combination of neural stem cells and FK506 can improve nerve regeneration. It may provide an expanded source for those afflicted with extensive nerve defects.     

Local effect of celecoxib on peripheral nerve repair com- bined with silicone tubulization in rat

Objective： To assess local effect of celecoxib on nerve regeneration in a rat sciatic nerve transec- tion model. Methods： Forty-five male healthy white Wistar rats were randomly divided into three experimental groups （n= 15 for each）： sham-operation （SHAM）, control （SIL） and celecoxib treated （SIL/CLX） groups. In SHAM group after anesthesia left sciatic nerve was exposed and after homeo- stasis muscle was sutured. In SIL group the left sciatic nerve was exposed in the same way and transected proximal to tibioperoneal bifurcation leaving a 10 mm gap. Proximal and distal stumps were each inserted into a silicone tube and filled with 10 gl phosphate buffered solution. In SIL/CLX group defect was bridged using a silicone tube filled with 10 μl celecoxib （0.1 g/L）. Results： Functional study and gastrocnemius muscle mass confirmed faster and better recovery of regenerated axons in SIL/CLX than in SIL group （P〈0.05）. Morphometric indices of regenerated fibers showed number and diameter of the myelinated fibers in SIL/CLX were significantly greater than those in control group. In immunohistochemistry, lo- cation of reactions to S-100 in SIL/CLX was clearly more positive than that in SIL group. Conclusion： Response to local treatment ofcelecoxib demonstrates that it influences and improves functional re- covery of peripheral nerve regeneration.     

三七总皂苷对移植静脉再狭窄的影响

目的 观察三七总皂苷(PNS)对大鼠移植静脉再狭窄的影响.方法 建立SD大鼠颈外静脉颈总动脉旁路移植模型,随机分为假手术组、模型组和PNS组,造模后第2天开始灌胃给药,PNS组给予PNS(100 mg/kg),假手术组、模型组灌胃等量蒸馏水(10 ml/kg).给药14 d后,取静脉血管桥固定,观察血管内膜增生,免疫组织化学测定增殖细胞核抗原(PCNA)表达及TUNEL法检测平滑肌细胞凋亡.结果 上述3组的内膜厚度分别为(4.05±0.85)、(26.30±1.15)、(10.80±0.98)μm;内膜/中膜分别为(0.22±0.09)、(0.76±0.27)、(0.45±0.23),PNS组新内膜厚度明显低于模型组(P＜0.05);模型组的PCNA表达量(A值)为(23.6±4.3),明显高于PNS组(11.5±3.6,P＜0.05);模型组的凋亡细胞百分数为(4.3±1.9),明显低于PNS组(20.3±3.5,P＜0.05).结论 PNS可以抑制静脉移植血管桥新生内膜的增生,对预防移植血管再狭窄有一定作用.

Abstract：

Objective To observate the effects of panax notoginseng saponin (PNS) on restenosis of rat vein graft.Methods Jugular vein-to-artery bypass grafting was performed on 30 Sprague-Dawley rats.The rats were divided into three groups: sham group, model group and PNS group.Drugs were administered at the second day after the operation for 14 days.The grafts were harvested for histochemical staining to observe the hyperplasia of neointima, and proliferating cell nuclear antigen (PCNA) expression,and apoptosis of smooth muscle cells were detected by TUNEL.Results In sham group, model group and PNS group, the endometrial thickness was (4.05 ±0.85), (26.30 ± 1.15), ( 10.80 ±0.98) μm; the intima/media was (0.22 0.09), (0.76 0.27), (0.45 0.23), respectively.The neointimal thickness in PNS group was significantly less than in model group ( P ＜ 0.05 ).The PCNA expression in model group (A value) was ( 23.6 ± 4.3 ), significantly higher than in PNS group ( 11.5 ± 3.6 ) ( P ＜ 0.05 ).The percentage of apoptotic cells in model group was (4.3 ± 1.9), significantly lower than in PNS group (20.3 ± 3.5 ) (P ＜ 0.05 ).Conclusion PNS can inhibit the neointimal hyperplais of vein graft, which can prevente restenosis after bypass grafts.     

纳米细菌感染致SD大鼠前列腺结石的实验研究

目的 建立前列腺纳米细菌(NB)感染的SD大鼠模型,探讨NB在前列腺炎以及前列腺结石形成中的致病作用.方法 40只成年雄性SD大鼠随机分为对照组和模型组,每组20只.分别经尿道注入生理盐水0.2 ml和NB混悬液0.2 ml(浓度为1个麦氏单位).将2组动物分别在4周、8周后处死(每次每组10只).HE染色观察2组大鼠前列腺组织的病理改变,透射电镜和扫描电镜观察有无结石形成.取前列腺组织做NB细菌的再培养和鉴定.结果 4周后模型组大鼠前列腺组织出现慢性炎症,并持续到8周后.8周后模型组7只大鼠前列腺组织中发现小结石.前列腺组织NB再培养均有NB生长.对照组动物前列腺组织未见炎症及结石,NB细菌的再培养未见NB生长.结论 NB感染大鼠前列腺能造成前列腺慢性炎症,并形成结石,NB感染可能是前列腺结石形成的重要病因.

Abstract：

Objective To reproduce an SD rat model of prostatic calculus by using nanobacteria (NB), and explore the role of NB in contributing to prostatitis and prostatic calculus. Methods Twenty adult male SD rats were randomized to the control group and 20 to the model group. Rat prostate infection models were reproduced by infusing 0. 2 ml (Concentration, 1 Mai unit) NB suspension transurethrally. 0.2 ml physiological saline was infused transurethrally in the rat control group. The rats were sacrificed 4 and 8 weeks later and prostatic pathology were viewed by hematoxylin and eosin (HE) staining. Lithogenesis was observed by scanning electron microscope (SEM) or Transmission electron microscopy (TEM). Re-isolation, culture and identification of nanobacteria were also done in rat prostatic tissues. Results Chronic inflammatory changes of prostates were shown in the model group at both 4 weeks and 8 weeks after infusing NB suspension. Prostatic calculi were detected by SEM and TEM at 8 weeks in the prostates of the rat model group (7/10). Neither chronic inflammatory changes nor prostatic calculus was found in the control group. NB was positive in the model group, but negative in the control group. Conclusions NB infection could cause chronic prostatitis and prostatic calculus in rats.     

成体神经干细胞脑内移植对脑外伤大鼠神经功能的影响

目的 观察脑内移植成体神经干细胞对脑外伤大鼠中枢神经功能的影响.方法 将60只Wistar大鼠随机分为5组,正常组(A)、模型组(B)、假手术组(C)、PBS移植组(D)、干细胞移植组(E).Morris水迷宫实验测试各组大鼠的学习记忆能力;免疫组织化学及酶联免疫吸附试验(ELISA)检测大鼠脑组织中脑源性神经生长因子(BDNF)、神经生长因子(NGF)的表达.结果与A组比较,B组大鼠寻找隐形平台的时间明显延长(P<0.01),脑组织中BDNF(27.70±1.65)ng/g蛋白、NGF(59.80±9.40)ng/g蛋白含量下降显著(P<0.01),模型复制效果理想;与B组比较,E组大鼠学习及功能明显改善,脑内BDNF(49.30±2.87)ng/g蛋白、NGF(88.50±9.23)ng/g蛋白的表达量上升,差异有统计学意义(P<0.01).结论 成体神经干细胞的脑内移植能明显恢复外伤大鼠中枢神经功能.

Abstract：

Objective To study the neuroprotetive effect of dault neural stem cells intracerebral implantation to rats subjected to traumatic brain injury.Methods Sixty Wistar rats were divided into 5 groups randomly: normal group ( A ),model group ( B),sham-operated group ( C ),PBS transplated group (D),neural stem cells transplated group (E).Morris maze method was adopted to observe the impact on the learning and memory.Immunohistochemisty and enzyme linked immunosorbent assay (ELISA)were used to detect the expression of brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in brain issue.Results As compared with group A,in Morris maze experiment,time to reach the end point of the maze in group B was significantly prolonged ( P ＜ 0.01 ),and the content of BDNF (27.70 ± 1.65 ) ng/g prot and NGF ( 59.80 ± 9.40 ) ng/g prot were decreased significantly ( P ＜ 0.01 ).As compared with group B,the function of learning and memory was obviously improved,and the expression of BDNF (49.30 ±2.87) ng/g prot and NGF (88.50 ±9.23) ng/gprot in brain issues were statistically increased in group E (P ＜0.01 ).Conclusion Transplantation of adult neural stem cells can ameliorate learning and memory deficits following traumatic brain injury in rats.     

The development of a rat model of erectile dysfunction after radical prostatectomy: preliminary findings

OBJECTIVE

To develop a rat model of erectile dysfunction (ED) after cavernous nerve injury.

MATERIALS AND METHODS

Given the great similarity between the anatomical structure of the cavernous nerve in rats and humans, 24 rats underwent dissections and the cavernous nerves were identified with the aid of an operating microscope. Then the rats were randomized into two groups: sham‐operated controls and a bilateral cavernous nerve section group. At 3 months after surgery, the rats were evaluated for their response to an apomorphine challenge.

RESULTS

The erectile response after an apomorphine challenge was normal in all the control rats, while there were no erections in the bilateral injured group.

CONCLUSION

The rat major autonomic ganglion and its cavernous nerve can be identified with the aid of a microscope. Rats are inexpensive and easy to handle, thus a good animal for developing an ED model of cavernous nerve injury. In the present study, the rats with cavernous nerve injury lost erectile capacity in a reliable and reproducible fashion. Because of the great similarity between the cavernous nerve of rats and humans, one may consider this technique as a reliable experimental model for studying ED after radical prostatectomy.     

Nerve injury-related erectile dysfunction following nerve-sparing radical prostatectomy: A novel experimental dissection model

Objectives:   To establish a new experimental rat model in order to define the mechanisms of erectile dysfunction (ED) and to evaluate the changes of neuronal nitric oxide synthase (nNOS) in the pelvic ganglia following nerve-sparing radical prostatectomy.
Methods:   Sprague-Dawley rats were randomized to sham operation, bilateral cavernous nerve dissection (BCND) and bilateral cavernous nerve resection (BCNR) groups. In the BCND group, the cavernous nerves were only dissected bilaterally from the major pelvic ganglion (MPG) to the apex of the prostate without crushing or cutting. At 1, 2, 4 and 8 weeks after surgery, we examined intracavernous pressure along with arterial pressure (ICP/AP), retrograde dye tracing using Fluorogold (FG) and expression of nNOS in the MPG.
Results:   Intracavernous pressure and arterial pressure in the BCND group was significantly decreased at 2 and 4 weeks after surgery compared with the sham group, and improved at 8 weeks. The number of FG-positive cells in the MPG also recovered at 8 weeks. ICP/AP and FG-positive cells in the BCNR group were greatly decreased until 8 weeks. The percentage of nNOS-positive cells per total cells was not different between the sham and BCND groups during the experimental period, whereas that in the BCNR group gradually decreased with time.
Conclusions:   We established a novel rat model, in which cavernous nerve dissection alone caused nerve injury-related ED. We believe that this cavernous nerve dissection model might help clarify the mechanism of nerve injury-related ED and the recovery from ED after nerve-sparing radical prostatectomy.     

海绵体神经损伤所致ED大鼠模型建立

目的 :寻找大鼠海绵体神经并建立神经损伤所致ED大鼠模型。　方法 :对 2 0只大鼠进行解剖 ,在外科显微镜下找到海绵体神经并经电刺激试验证实。随后将 4 2只实验大鼠随机分为假手术对照组、单侧海绵体神经损伤组及双侧海绵体神经损伤组。术后 3周用阿朴吗啡试验来评估所建动物模型。　结果 :盆大神经节位于背侧前列腺后外侧叶表面 ,海绵体神经是最大的传出神经。诱发阴茎勃起的电刺激参数 :电压 5V、刺激频率 2 0Hz及刺激时间 5ms。术后 3周 ,阿朴吗啡均能诱发对照组大鼠阴茎勃起 ,30min内平均勃起 (2 5 7± 1 4 0 )次。实验组大鼠 ,无论单侧损伤还是双侧损伤 ,均丧失勃起功能 (0 0 0± 0 0 0 )。　结论 :大鼠较大的盆大神经节及海绵体神经易于辨认 ,电刺激反应明显 ,是建立海绵体神经损伤性ED模型的理想动物。无论是单侧海绵体神经损伤还是双侧海绵体损伤 ,损伤早期 ,大鼠均丧失勃起功能     

Blunt cavernous nerve injury: A new animal model mimicking postradical prostatectomy neurogenic impotence

Our goal was to develop an animal model of cavernous nerve injury similar to that encountered among patients having undergone a successful nerve sparing radical prostatectomy and to compare patterns of nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase staining to quality of erections using the newly developed model. We studied 50 mature Sprague Dawley rats, which were divided into five equal groups. Animals were either observed (sham), underwent an exploratory laparotomy, underwent moderate or severe percussive injury to both cavernous nerves, or underwent ablation of both cavernous nerves. Between 28 and 30 days later, all animals underwent electrostimulation and simultaneous recording of intracavernosal pressure. After sacrifice, penes were harvested and penile tissue NADPH-diaphorase staining pattern was assessed. Severity of cavernous nerve percussive injury and NADPH-diaphorase staining patterns correlated with the quality of recorded erections. This model is a useful experimental tool for research in the field of erectile dysfunction such as is encountered following a successful nerve sparing radical prostatectomy. Penile biopsy assessing NADPH-diaphorase staining may potentially prove to be a useful minimally-invasive diagnostic modality quantifying neurogenic erectile function among patients following radical prostatectomy.     

红景Ⅰ号方对双侧海绵体神经损伤SD大鼠阴茎组织中缝隙连接蛋白43表达的影响

目的:探索红景Ⅰ号方对双侧海绵体神经损伤大鼠勃起功能及其阴茎组织中缝隙连接蛋白43的干预作用. 方法:将50只SD大鼠随机均分为5组,分别为假手术组、双侧海绵体神经损伤组、红景Ⅰ号方低、中、高剂量组.利用止血钳钳夹大鼠双侧海绵体神经以造成损伤,红景Ⅰ号方组给予不同剂量(2.835、5.67、11.34g/kg)灌胃.2...     

低氧分压对大鼠勃起功能的影响

目的探讨低氧分压对大鼠勃起功能障碍（ED）的影响。方法48只成年白色雄性SD大鼠随机分为对照组和实验组，每组按照实验时间（2周、6周、10周）分为3个亚组，每个亚组8只。实验组置于密闭低氧舱中饲养，对照组在正常环境中饲养，其他条件相同。分别观察其勃起功能，采用免疫组化SP法检测神经源性一氧化氮合成酶（nNOS）阳性神经纤维的数量、内皮源性一氧化氮合成酶（eNOS）的表达。结果实验组与对照组比较：（1）大鼠勃起次数明显降低（P〈0．001）；（2）nNOS阳性神经纤维数量、eNOS蛋白的表达均有显著性差异（P〈0．01）。实验组间比较：勃起次数6周组明显低于2周组，10周组稍高于6周组；nNOS阳性神经纤维数量有显著性差异（P〈0．01）；eNOS的表达6周组最低，10周组有所回升。结论低氧分压致大鼠勃起功能受损和nNOS、eNOS的表达下降。nNOS染色阳性神经纤维数量的减少、eNOS表达的下降，可能是低氧分压环境中大鼠ED发生的原因之一。     

神经干细胞移植重建勃起功能的研究

目的 观察神经干细胞移植修复损伤的海绵体神经从而恢复勃起功能的可行性.方法 42只雄性SD大鼠(3～4个月龄和体质量300～400 g)随机分为假手术组、神经干细胞移植组和神经损伤组,每组14只.2、4个月后,海绵体神经电刺激检测大鼠阴茎勃起功能,免疫组织化学法检测海绵体内一氧化氮合酶(nNOS)阳性神经纤维.结果 2个月后3组大鼠对海绵体神经电刺激的勃起反应率分别为100%、0%和0%;3组海绵体内nNOS阳性神经纤维数目分别为98.5±9.2、22.5±3.5和25.7±5.1,后2组间差异无统计学意义(P>0.05).4个月后3组大鼠电刺激后勃起率分别为100%、57.14%和7.19%,后2组间差异有统计学意义(P<0.05);3组海绵体内nNOS阳性神经纤维数目分别为95.1±7.7、86.0±13.4、26.5±4.3,前2组间差异无统计学意义(P>0.05),后2组间差异有统计学意义(P<0.05).结论 神经干细胞移植是修复损伤的海绵体神经从而恢复勃起功能的一种有效方法.     

Growth hormone enhances regeneration of cavernous nerves after their transplantation in rats]

OBJECTIVE: To investigate the effect of growth hormone (GH) on penile erection after reconstruction of cavernous nerves using sural nerve as an interposition nerve graft in rats. METHODS: Twenty-four male Sprague-Dawley rats (3-4 ms of age and 300-400 g in weight) were randomly divided into 2 groups: nerve graft group and GH group, each electrostimulated to determine the erectile potency 2 and 4 months after nerve graft (followed by hypodermic GH injection). The nNOS-positive nerve fibers in the corpora cavemosa were examined by streptavidin-peroxidase immunohistochemistry technique (SP method). Image analysis was used to calculate the area stained in pixel. RESULTS: Electrostimulation at 2 months produced 31.25% of erections in the GH group but none in the grafted rats. There was a significant difference in the erection rate produced by electrostimulation between the two groups at 2 months (P < 0.05). The pixel of the expression of nNOS-positive nerve fibers in the GH group (38971 +/- 7692) was also greater than that of the graft group (16538 +/- 3179, P < 0.05). At 4 months, 43.75% of the graft group and 75% of the GH group produced erections upon electrostimulation, with no significant difference between the two groups (P > 0.05). The pixels of the expression of nNOS-positive nerve fibers were 79276 +/- 12,021 and 91348 +/- 18965, respectively (P > 0.05). CONCLUSION: GH can accelerate the regeneration of cavernous nerves after bilateral nerve grafting, and GH administration may present a new physiological approach to the treatment of erectile dysfunction after radical pelvic surgery.