人烧伤创面应用表皮生长因子的临床评价

应用ＥＧＦ于人的Ⅱ度、深Ⅱ度烧伤创面和刃厚供皮区创面，观察其出现上皮组织时间和创面完全愈合时间。结果：用ＥＧＦ的浅Ⅱ度烧伤创面和刃厚供皮区创面比对照创面早愈合３ｄ左右（Ｐ＜０．０５），而用ＥＧＦ的深Ⅱ度烧伤创面与对照创面愈合时间没有明显差异（Ｐ＞０．００５）。     

Structural bone allografts with intramedullary vascularized fibular autografts for the treatment of massive bone defects in extremities

Objective：To report the clinical outcome of repairing massive bone defects biologically in limbs by homeochronous using structural bone allografts with intramedullary vascularized fibular autografts. Methods： From January 2001 to December 2005, large bone defects in 19 patients （11 men and 8 women, aged 6 to 35 years） were repaired by structural bone allografts with intramedullary vascularized fibular autografts in the homeochronous period. The range of the length of bone defects was 11 to 25 cm （mean 17.6 cm）, length of vascularized free fibular was 15 to 29 cm （mean 19.2 cm）, length of massive bone allografts was 11 to 24 cm （mean 17.1 cm）. Location of massive bone defects was in humerus（n=1）, in femur（n=9） and in tibia（n=9）, respectively. Results： After 9 to 69 months （mean 38.2 months） follow-up, wounds of donor and recipient sites were healed inⅠstage, monitoring-flaps were alive, eject reaction of massive bone allografts were slight, no complications in donor limbs. Fifteen patients had the evidence of radiographic union 3 to 6 months after surgery, 3 cases united 8 months later, and the remained one case of malignant synovioma in distal femur recurred and amputated the leg 2.5 months, postoperatively. Five patients had been removed internal fixation, complete bone unions were found one year postoperatively. None of massive bone allografts were absorbed or collapsed at last follow-up. Conclusion： The homeochronous usage of structural bone allograft with an intramedullary vascularized fibular autograft can biologically obtain a structure with the immediate mechanical strength of the allograft, a potential result of revascularization through the vascularized fibula, and accelerate bone union not only between fibular autograft and the host but also between massive bone allograft and the host.     

Identification of cultured male epidermal allografts by detecting Y chromosome DNA fragments in female burn victims

ltiswellknownthattheskinofextensivelyburnedcasesthemselvesisinsufficienttoprovidecoverageforwounds.Thoughallogeneicskincancoverthewoundstemporari1y,itwillberejectedwithin2-3weeks.Thiscanthreatenthepatient'slifeifthereisnoenoughautogenousskintoreplacetherejectedoneontime.GreenetalL1]reportedthattheburnwoundsofpatientswithextensiveburnscouldbetreatedbytransplantingepidermalautograftsafterautologousepidermalcellsmulti-plyforthousandsoftimesinvltrowithinashortperiodoftime-Butittakesatleast2-3weeks…     

阴囊皮瓣在阴茎创面修复中的运用

目的探讨阴囊皮瓣在阴茎创面修复中的作用。方法采用阴囊任意皮瓣修复皮肤软组织缺损21例,清洁创面采取一期修复,污染创面彻底清创后修复,感染创面待感染控制、坏死组织完全脱落后二期修复,供瓣区均直接缝合。结果1例因排尿困难发生伤口裂开,其余20例皮瓣全部成活,切口一期愈合。术后随访6个月~6年,皮瓣修复的创面局部弹性良好,无1例发生勃起功能及排尿功能障碍,远期效果较好。结论阴囊皮肤血供丰富,伸展性好,阴囊任意皮瓣长:宽达3:1仍能较好存活。可修复阴茎任意部位创面,供瓣区一般能直接缝合,术后阴茎阴囊功能均未受较大影响,是简单易行、安全有效的阴茎创面修复的方法之一。     

THE EFFECT OF SKIN GRAFTING AT DIFFERENT TIME POST BLOOD TRANSFUSION AND BONE MARROW TRANSPLANTATION ON RATS COMBINED RADIATION——BURN INJURY

After the rats were iufficted with 8Gy total body gamma ray irradiation and 15% total body surface area (TBSA) full thickness burn injury, they were treated with blood transfusion (BT) and bone marrow transplantation (BMT). Then the survival of allografts grafted on the eschareetomized burn wounds in the 24, 48 and 72 h postinjury was observed. It was found that when the burn wounds were closed with allografts in the 24h postinjury, there were an early elevation of leucocytes, the appearance of the donor‘‘s cells and a significantly higher survival rate of the rats on the 30 day postinjury. The allogaafts could survive longer and wounds showed no signs of infection and healed quicker. When the allografts were grafted in the 48 h or 72 h postinjury, only harmful effects to hasten the death of rats were observed.     

封闭式负压引流技术在各种复杂创面治疗中的应用

目的 总结封闭式负压引流(VAC)技术治疗各种创面的效果,进一探讨该技术的适应证与禁忌证.方法 2004年1月至2015年8月,笔者应用VAC治疗各种创面357例,其中创伤后皮肤软组织缺损113例、糖尿病溃疡和足趾坏疽74例,骨髓炎创面14例、术后伤口愈合不良22例、压力性溃疡37例、跟腱术后外露18例、窦道11例、Ⅲ度烧伤伴脓肿和慢性溃疡形成15例,Ⅲ度电击伤8例、供皮区5例、血管渗漏组织坏死2例、贯通伤1例,植皮区12例,下肢静脉性溃疡7例,骨筋膜室综合征5例,热压伤5例,痛风性溃疡2例,外周动脉性溃疡5例,不明原因慢性溃疡1例.积极全身支持治疗,及时手术扩创,行VAC治疗,术后3~7 d更换负压装置.结果 经过VAC治疗后,植皮区皮片全部存活;余创面中,除供皮区、动脉疾病性溃疡外,其他创面肉芽组织生长迅速,全部或部分覆盖深部组织,创面缩小.自行愈合6例,直接扩创缝合34例,自体皮片移植修复167例,皮瓣修复128例,人工真皮联合自体皮片复合移植修复19例,3例创面缩小后改传统换药治疗.患者随访6个月~3年,除3例糖尿病治愈患者同部位再次出现创面外,其他无复发,3例仍有残余创面,总治愈率为98.3%.结论 VAC适应证较广,对多数复杂难愈创面有显著效果,能明显缩短疗程,提高治愈率,但供皮区等创面效果不佳.     

局部CTLA4Ig转基因治疗大鼠小肠移植排斥

目的 :研究CTLA4Ig基因在小肠局部转染表达及其表达产物对小肠移植物存活的作用。方法 :移植前经肠系膜上动脉供给肠灌注脂质体包裹的CTLA4IgcDNA ,应用免疫组织学及RT PCR观察移植小肠中CTLA4Ig转基因产物的表达。结果 :免疫组织学及RT PCR结果显示在移植后 2 8天内移植小肠中有CTLA4Ig转基因产物的表达 ,18例移植小肠中的 11例在受体内的存活时间超过 90天。结论 :经肠系膜上动脉体外灌注脂质体包裹的CTLA4IgcDNA可有效转染移植小肠 ,转染的移植小肠在受体内可长时间存活     

重组人生长激素治疗严重烧伤病人临床研究

目的:探讨生长激素对成人烧伤创面愈合时间、住院天数,用药前后白蛋白、BUN的变化,用药前后免疫蛋白含量变化。方法:选择2006年1月~2008年2月间30例严重烧伤患者为研究对象,并随机分为重组人生长激素组(r HG Hgroup)和对照组(control group),动态监测创面愈合时间、住院天数、蛋白、BUN、体重、免疫蛋白多项实验室指标。结果:创面愈合时间,住院天数,蛋白质的合成,提高烧伤后机体免疫力等r HGH组均优于对照组。结论:使用r HGH有助于改善全身状况,促进创面愈合,减少并发症。     

人工真皮支架在大面积撕脱伤救治中的应用

目的 研究人工真皮支架在大面积撕脱伤救治中的应用。方法 回顾性分析第二军医大学长海医院烧创伤外科2011年1月至2014年12月收治的大面积撕脱伤患者20例,年龄6~68岁,撕脱面积占总体表面积（total body surface area,TBSA）10%~40%。按照治疗方法不同将患者分为两组,每组各10例,对照组患者一期行清创+负压引流术,二期行取植皮术;治疗组患者一期行清创+真皮支架+负压引流术,5~7 d后更换负压装置,观察基底血管化程度,伤后12~14 d行取植皮术。分别统计两组患者从清创到植皮的间隔时间,供皮区愈合时间和愈合质量以及植皮区皮片成活率、愈合时间及愈合质量。结果 与对照组相比,治疗组患者从清创到植皮的间隔时间延长[（13.30±2.06）d vs（7.90±1.10）d,P<0.01];供皮区愈合时间缩短[（8.10±0.99）d vs（13.10±1.10）d,P<0.01],供皮区愈合质量提升（4.40±1.58 vs 7.80±1.14,P<0.01）;植皮区皮片成活率[（87.30±5.27）% vs（85.10±5.53）%]、愈合时间[（17.80±1.14）d vs（18.70±2.06）d]、愈合质量（8.40±1.07 vs 9.00±1.05）差异均无统计学意义（P>0.05）。结论 在大面积撕脱伤的治疗过程中早期应用真皮支架联合负压引流技术,可避免在伤后1周左右大范围取皮,手术损伤少,对全身干扰小,可保证患者平稳度过病程早期阶段;同时通过支架自身的血管化可促进创面恢复,减少植皮所需皮片厚度,从而缩短供皮区愈合时间、提高供皮区愈合质量。     

局部应用外源性生长因子对糖尿病创面愈合影响的临床研究

目的 :从临床角度观察局部应用外源性生长因子对糖尿病创面愈合的影响。方法 :局部应用外源性生长因子于糖尿病创面 12例 ,观察术后 3、7、14 d创面上皮葡行后残余面积及肉芽成熟程度 ,同时在同一部位另一创面做空白对照 ,并与非糖尿病创面愈合过程进行比较。结果 :糖尿病创面应用外源性生长因子与未应用生长因子与非糖尿病创面比较 ,其上皮葡行速度在早期未见明显差异 ,在后期其上皮葡行速度由快至慢顺序为非糖尿病创面应用生长因子 >糖尿病创面应用生长因子 >非糖尿病创面未应用生长因子 >糖尿病创面未应用生长因子。局部应用外源性生长因子可促进糖尿病创面上皮化以及肉芽组织的生长 ,但糖尿病创面肉芽组织的生长明显滞后于非糖尿病创面。结论 :局部应用外源性生长因子对糖尿病创面愈合具有促进作用。