脂质过氧化对血管内皮细胞源性生长因子分泌的影响

脂质过氧化对血管内皮细胞源性生长因子分泌的影响杨向红陈铁镇一、材料和方法（１）牛主动脉内皮细胞的培养：于无菌条件下取牛主动脉，以消化法进行内皮细胞的原代培养。培养液为含１０％小牛血清的ＤＭＥＭ培养液。第２～５代继代细胞用于实验。（２）脂质过氧化实验：...     

冠心病患者血清过氧化脂质的变化及其与动脉硬化关系的研究

对３０例不稳定型心绞痛（ＵＡ）、１９例急性心肌梗塞（ＡＭＩ）患者血清过氧化脂质（ＬＰＯ）、血清总胆固醇（ＴＣ）、甘油三酯（ＴＧ）、高密度脂蛋白胆固醇（ＨＤＬ－ｃ）进行了测定，并计算动脉硬化指数（ＡＩ）。发现ＵＡ及ＡＭＩ患者ＬＰＯ、ＴＣ、ＴＧ、ＡＩ值均显著高于正常人，ＨＤＬ－ｃ均低于正常人。ＵＡ与ＡＭＩ患者血清ＬＰＯ及血脂诸值比较差异均无显著性。ＵＡ与ＡＭＩ两组患者的ＬＰＯ水平均与ＡＩ呈显著正相关。     

大蒜素对血管内皮细胞脂质过氧化损伤的保护作用

目的 :观察大蒜素对培养的人脐静脉内皮细胞 (HUVEC)脂质过氧化损伤的保护作用。方法 :将HUVEC分成空白组、对照组和实验组 ,实验组分别用终浓度为 5 μg/ml、10 μg/ml、2 0 μg/ml、5 0 μg/ml的大蒜素培养液预处理后 ,再加入浓度为 0 .0 6mmol/L的联胺 37℃孵育 2h ,用扫描电镜观察细胞的形态 ,同时测定细胞培养液中一氧化氮 (NO)、丙二醛 (MDA)和超氧化物歧化酶 (SOD)的含量。结果 :对照组HUVEC明显受损 ,MDA含量增加 (P <0 .0 1) ,NO、SOD减少 (均为P <0 .0 1) ;实验组与对照组比较 ,HUVEC损伤明显减轻 ,MDA含量降低 (P <0 .0 1) ,NO增加 (P <0 .0 1)、SOD增加 (P <0 .0 1) ,且呈剂量依赖性。结论 :大蒜素对HUVEC脂质过氧化损害具有保护作用。     

脂质过氧化对人内皮细胞的损伤及抗氧化剂的保护作用

以氢过氧化估烯作为培养人内皮细胞脂质过氧化反应的引发剂，以亚硒酸钠、维生素E、超氧化物歧化酶作为抗氧剂，应用光镜、脂质过氧化物测定及放射免疫分析等方法观察了抗氧剂对脂质过氧化损伤的内皮细胞形态结构和功能的保护作用。结果表明：氢过氧化枯烯激发和促进了内皮细胞的脂质过氧化反应；而硒、维生素E、超氧化物歧化酶使细胞脂质过氧化物含量减少，由过氧化作用造成的细胞收缩、生物膜系统的损伤程度减轻，对细胞前列腺素（PGI2）合成的抑制作用减弱。提示：上述抗氧剂可能通过对内皮细胞的保护作用，阻止动脉粥样硬化病变的形成和发展。     

脂质过氧化对人血管内皮细胞NO合成的影响及维生素E的作用

一、材料与方法1.细胞培养 :人脐静脉内皮细胞 (ＨＵＶＥＣ)培养采用本实验室改进的Ｊａｆｆｅ培养法。经免疫荧光检测第Ⅷ因子相关抗原阳性 ,鉴定为血管内皮细胞。2 .实验分组 :分 8组 ,第 1组为正常对照组 ;第 2组为氢过氧化枯烯 0 0 1ｍｍｏｌ/Ｌ ;第 3组为氢过氧化枯烯 0 0 5ｍｍｏｌ/Ｌ ;第 4组为氢过氧化枯烯 0 10ｍｍｏｌ/Ｌ ;第 5组为氢过氧化枯烯 0 5 0ｍｍｏｌ/Ｌ ;第 6组为氢过氧化枯烯 1 0 0ｍｍｏｌ/Ｌ ;第 7组为氢过氧化枯烯 0 5 0ｍｍｏｌ/Ｌ +维生素Ｅ(ＶｉｔＥ) 5 0ｍｇ/Ｌ ;第 8组为氢过氧化枯烯 1 0 0ｍｍｏｌ/Ｌ +…     

高脂血症,脂质过氧化,抗氧化酶活性与动脉粥样硬化的关系

用大剂量胆固醇(1.5g/日)喂家兔60天后停胆固醇30天塑造动脉粥样硬化(AS)模型。观察血胆固醇、过氧化脂(LPO)含量和抗氧化酶活性与AS病变发生发展的关系。发现:血清胆固醇水平随喂胆固醇时间延长而升高,至60天时达高峰,停饲胆固醇,血清胆固醇水平迅速下降,而同样升高的LPO水平不但未降,反而继续升高,明显高于对照的水平。抗氧化酶SOD和GSH-Px活性在LPO升高的早期显示代偿性增高,以后即降低并保持在低于对照的水平。主动脉、肺动脉和冠状动脉均发生程度不等的AS病变,即使在停饲胆固醇一个月后亦可见进行性病变,如大量平滑肌细胞增生和炎细胞浸润,且其病变较前更重。以上结果提示,血中过量的LPO抑制了抗氧化酶活性,可能在AS的发生发展中起重要作用。     

NIDDM患者红细胞膜脂质过氧化作用与红细胞免疫功能之间的关系

对58例非胰岛素依赖型糖尿病(NIDDM)患者检测了红细胞免疫指标,同时测定了血浆和红细胞膜脂质过氧化水平。结果显示,NIDDM患者红细胞C3b受体活性明显降低,血浆和红细胞膜脂质过氧化水平明显升高;在NIDDM有血管并发症者上述变化更为显著,同时伴有红细胞免疫复合物花环率降低;红细胞C3b受体活性降低和血浆及红细胞膜脂质过氧化水平升高呈负相关,进一步多元逐步回归分析红细胞腆脂质过氧化水平升高和红细胞C3b受体活性降低关系更为密切。提示红细胞膜脂质过氧化损坏可能是引起红细胞免疫功能降低的一重要因素。     

万爽力改善稳定性冠心病患者内皮细胞功能的临床观察

目的 探讨万爽力(曲美他嗪)对稳定性冠心病患者内皮细胞功能的影响。方法 对61例稳定性冠心病患者采用随机、单盲、对照的方法分为两组,一组为常规治疗组(n=30),另一组为万爽力组(n=31),连续治疗8周。治疗前后静脉抽血,采用放射免疫法测定血浆中的肾上腺髓质素(ADM)及内皮素-1(ET-1)的浓度。结果 万爽力组治疗后ADM浓度明显上升(P<0.05),同时ET-1浓度明显下降(P<0.05),且与常规治疗组比较有显著差异(P<0.05)。结论 万爽力可改善稳定性冠心病中层得内皮细胞功能。     

Dermatoclyphic Patterns in Angiographically Proven Coronary Artery Disease

Dermatoglyphics is the scientific study of epidermal ridges and their configurations on the palmar region of hand and fingers and plantar region of foot and toes. Against the genetic background of dermatoglyphic patterns and coronary artery disease, the study was undertaken to determine the correlation between them. The present study includes 150 cases of angiographically proven coronary artery disease (CAD) and 150 cases of healthy normal individuals. The palmar prints of both hands were taken on white paper by Ink method as described by Cummins and Midlo. It is noted that there is significant decrease in loops with corresponding increase in whorls in males, (M+F) combined series and left hand of CAD patients. The true palmar pattern is significantly decreased in thenar area in females; third interdigital area in (M+F) combined series and right hand; and fourth interdigital area in left hand of CAD patients. There is significant increase in ‘4’ palmar triradii in males, (M+F) combined series and right hand; and ‘5’ palmar triradii in females with significant decrease in ‘6’ palmar triradii in both sexes and both hands of CAD patients. The mean value of atd angle is significantly increased in males, (M+F) combined series and left hand of CAD patients as compared to the controls. There is no significant correlation in ab ridge count and CAD.     

Association Patterns of Endothelial Nitric Oxide Synthase Gene (NOS3) Variant Glu298Asp with Blood Pressure and Serum Lipid Levels in Subjects with Coronary Artery Disease from Pakistan

Nitric oxide is an important antiatherosclerotic agent. The main determinant of nitric oxide levels is enzyme nitric oxide synthase encoded by the NOS3 gene, the common variants in this gene may be responsible for variations in plasma enzyme levels. The association of NOS3 variants with coronary artery disease (CAD) varies in different ethnicities. The current study aimed to determine the association of NOS3 Glu298Asp (rs1799983) with CAD and blood lipid levels in Pakistani subjects. Six hundred thirty‐six samples (412 cases, 224 controls) were genotyped by TaqMan allelic discrimination assay and serum total cholesterol, and High Density Lipoprotein cholesterol (HDL‐C)/Low Density Lipoprotein cholesterol (LDL‐C) and triglycerides were measured. The genotype frequency was Glu/Glu = 64.6%, Glu/Asp = 30.1%, and Asp/Asp = 5.3% in cases, and Glu/Glu = 68.8%, Glu/Asp = 26.7%, and Asp/Asp = 4.5% in controls. The Asp298 (T) frequency was not significantly higher in cases than controls (20.4% vs 17.9%, P = 0.28) and risk allele was not associated with CAD (OR 1.15 (0.86–1.54), P = 0.33) and the tested lipid traits but had a strong association with blood pressure (for systolic and diastolic P = 1.9×10^?–56 and 4×10^?–40, respectively). In conclusion, although Glu298Asp did not show association with CAD and lipid profile in the studied cohort, it may exert its effect through blood pressure; however, the mechanism of this effect needs to be explored in the future.     

血脂异常与老年冠心病患者冠脉病变程度的相关性研究

目的 探讨血脂异常与老年冠心病患者冠脉病变程度的相关性。方法 选取2015年5月~2017年1月本院心内科住院的明确诊断为冠状动脉粥样硬化性心脏病（CHD）的150例老年患者设为CHD组。选取同期收治的150例非CHD患者为对照组。比较两组血清中总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）水平,并计算N-HDL-C、LDL-C/N-HDL-C、LDL-C/HDL-C、TC/HDL-C、TG/HDL-C值。比较不同冠脉病变支数、不同病情严重程度的CHD患者的血脂及其比值。对血脂与冠脉病变支数、病情严重程度积分进行相关性分析。结果 CHD组患者LDL-C、TG、TC、N-HDL-C水平、LDL-C/HDL-C、TC/HDL-C、TG/HDL-C值均高于对照组,HDL-C水平低于对照组,差异均有统计学意义（P＜0.05）;不同冠脉病变支数各亚组间的TC、TG、N-HDL-C水平、TC/HDL-C比值差异存在统计学意义（P＜0.05）;TC、TG、N-HDL-C水平、TC/HDL-C比值均与冠脉病变支数均呈正相关（P＜0.05）;不同病情严重程度亚组间的LDL-C、TC、TG、N-HDL-C水平、LDL-C/HDL-C、TC/HDL-C、TG/HDL-C比值差异存在统计学意义（P＜0.05）;CHD患者LDL-C、TC、TG、N-HDL-C水平、LDL-C/HDL-C、TC/HDL-C、TG/HDL-C比值与冠状动脉病变积分均呈正相关（P＜0.05）。结论 可以通过对血脂指标的监测,并计算N-HDL-C、TC/HDL-C、LDL-C/HDL-C、TC/HDL-C、TG/HDL-C值,来评估CHD病情的临床进展。     

Fat-to-muscle Ratio: A New Indicator for Coronary Artery Disease in Healthy Adults

Background: Coronary artery disease (CAD) is an important issue in public health. Previous studies have shown that the ratio of fat to muscle mass is a significant predictor of metabolic disease, and it is known to be associated with atherosclerosis. In this study, we evaluated the association between the fat-to-muscle ratio (FMR) and CAD in healthy adults.Methods: A total of 617 participants without diabetes mellitus, hypertension, known CAD, or stroke who visited the Health Promotion Center from 2009 to 2018 were included in this study. Computed tomography imaging and bioelectrical impedance analysis were used to ascertain the coronary artery calcium (CAC) score, degree of CAD, and FMR.Results: Univariate logistic regression analysis showed that old age, male sex, smoking history, creatinine, aspartate aminotransferase, gamma-glutamyl transferase, uric acid, total cholesterol, and low-density lipoprotein cholesterol were significantly associated with CAC. After adjusting for potential confounding covariates, the presence of CAC was independently associated with FMR (OR, 1.014; 95% CI, 1.002-1.026; p = 0.019. The association was maintained even after adjusting for body mass index and waist circumference (odds ratio, 1.019; 95% confidence interval, 1.004 -1.034; P = 0.012).Conclusion: In this study, a high FMR was significantly associated with CAC. A large-scale prospective study on the association with FMR and cardiovascular diseases is necessary to confirm this relationship.     

血浆Hcy水平与冠心病的关系

目的：探讨血浆同型半胱氨酸（Hcy）水平与冠心病（CHD）的关系。方法：将109例CHD患者分为三组,无症状型CHD组26例,稳定型心绞痛组（SA）45例,心肌梗死组（MI）38例,对照组为40例健康体检者。以微粒子酶免分析法检测其空腹血浆Hcy水平。同时根据冠脉造影进一步将观察对象分为一支病变组（46例）,二支病变组（25例）和多支病变组（38例）。结果：CHD患者血浆Hcy水平显著高于对照组（P〈0．01）,MI组显著高于AS组（P〈0．05）,AS组高于无症状型CHD组（P〈0．05）;多支病变组Hcy水平显著高于双支病变组（P〈0．01）,双支病变组高于一支病变组（P〈0．05）。结论：CHD患者血浆Hcy水平明显增高,且与冠状动脉病变程度及冠脉病变支数呈正相关,是CHD的危险因素之一。     

冠心病冠脉病变严重程度与红细胞分布宽度及尿酸的相关性分析

目的 探讨冠心病冠脉病变严重程度与红细胞分布宽度及血尿酸的相关性。方法 选取中医药大学第一附属南院心血管病区2016年12月~2017年12月因胸痛疑诊冠心病或已明确冠心病患者134例,根据既往或入院后冠状动脉造影结果,左主干、左前降支、左回旋支、右冠状动脉或其主要分支血管直径狭窄≥50％者为冠心病组共96例,上述主要分支血管直径狭窄＜50％者共40例为对照组。根据冠状动脉病变支数分为单支病变组、双支病变组和三支病变组,采用Gensini法分别对冠脉病变进行评分。结果 冠脉病变严重程度与红细胞分布宽度与血尿酸水平呈正相关,尿酸（OR=1.006,P＜0.05）和红细胞分布宽度（OR=2.450,P＜0.05）的升高为冠心病的独立危险因素,且红细胞分布宽度与UA水平随着冠脉病变支数的增加逐渐递增,三支病变组红细胞分布宽度、血尿酸水平高于单支病变组（P=0.004、0.016＜0.05）,三支病变组红细胞分布宽度水平高于双支病变组（P＜0.05）;双支病变组红细胞分布宽度、血尿酸水平高于单支病变组,三支病变组血尿酸水平高于双支病变组,差异无统计学意义（P＞0.05）,考虑与样本量较小及患者用药控制因素有关。结论 冠心病冠脉病变严重程度与红细胞分布宽度及血尿酸呈正相关。     

Correlations between Oxidative DNA Damage, Oxidative Stress and Coenzyme Q10 in Patients with Coronary Artery Disease

The correlation of coronary artery disease (CAD) with pro-oxidant/antioxidant balance and oxidative DNA damage was investigated.Seventy-seven patients with CAD and 44 healthy individuals as control were included in this study. The comparative ratios of ubiquinol-10/ubiquinone-10, 8-hydroxy-2^''-deoxyguanosine/deoxyguanosine and the level of MDA measured by HPLC and the activities of GPX and SOD by colorimetric approach in blood samples obtained from patients with CAD were unraveled.8-OHdG/dG ratios, serum MDA level and GPX activity were found significantly elevated level in serum of CAD patients compared to control group. The SOD activity was observed in stable levels in CAD patients. Ubiquinol-10/ubiquinone-10 ratio was significantly lower in patients with CAD than the controls.The positive correlation was observed between 8-OHdG/dG ratios in both MDA levels and GPX activity, while the significant negative correlation was seemed between the ratio of 8-OHdG/dG and ubiquinol-10/ ubiquinone-10 as well as MDA levels and ubiquinol-10/ ubiquinone-10 ratio.We conclude that, both the disruption of pro-oxidant/antioxidant balance and oxidative stress in DNA may play an important role in the pathogenesis of coronary artery disease.     

Early Repolarization and Myocardial Scar Predict Poorest Prognosis in Patients with Coronary Artery Disease

Purpose

Recent studies show positive association of early repolarization (ER) with the risk of life-threatening arrhythmias in patients with coronary artery disease (CAD). This study was to investigate the relationships of ER with myocardial scarring and prognosis in patients with CAD.

Materials and Methods

Of 570 consecutive CAD patients, patients with and without ER were assigned to ER group (n=139) and no ER group (n=431), respectively. Myocardial scar was evaluated using cardiac single-photon emission computed tomography.

Results

ER group had previous history of myocardial infarction (33% vs. 15%, p<0.001) and lower left ventricular ejection fraction (57±13% vs. 62±13%, p<0.001) more frequently than no-ER group. While 74 (53%) patients in ER group had myocardial scar, only 121 (28%) patients had in no-ER group (p<0.001). During follow up, 9 (7%) and 4 (0.9%) patients had cardiac events in ER and no-ER group, respectively (p=0.001). All patients with cardiac events had ER in inferior leads and horizontal/descending ST-segment. Patients with both ER in inferior leads and horizontal/descending ST variant and scar had an increased adjusted hazard ratio of cardiac events (hazard ratio 16.0; 95% confidence interval: 4.1 to 55.8; p<0.001).

Conclusion

ER in inferior leads with a horizontal/descending ST variant was associated with increased risk of cardiac events. These findings suggest that ER in patients with CAD may be related to myocardial scar rather than pure ion channel problem.