肥胖及2型糖尿病患者血清肿瘤坏死因子相关蛋白9水平及二甲双胍对其的影响

目的：探讨肥胖及2型糖尿病（T2DM）患者血清脂肪肪细胞因子 C1q/肿瘤坏死因子相关蛋白9（CTRP 9）水平的变化,观察二甲双胍对其的影响。方法86例新诊断 T2DM 患者和88例正常糖耐量（NGT）者,按体质指数（BMI）又各自分为正常体重（NW）与肥胖（OB）亚组。采用 ELISA 法检测血清 CTRP9水平,观察二甲双胍对其水平的影响。结果（1）4组血清 CTRP9水平依次为：T2DM - OB 组＜ T2DM - NW 组、NGT - OB 组＜NGT - NW 组（P ＜0.05,P ＜0.01）;（2）NGT - OB 组 CTRP9与 FPG、HbA1C、HOMA - IR、BMI、TG 呈负相关（r ＝-0.603、-0.598、-0.595、-0.617、-0.512,P ＜0.05,P ＜0.01）,与 TNF -α、SOD、APN 呈正相关（ r ＝0.638、0.528、0.512,P ＜0.05,P ＜0.01）;T2DM 组 CTRP 9与 FPG、HbA1C、HOMA - IR、BMI、TG、TNF -α呈负相关（r ＝-0.596、-0.612、-0.586、-0.603、-0.531、-0.598,P ＜0.05,P ＜0.01）,与 APN、SOD 呈正相关（ r ＝0.552、0.512,P ＜0.05,P ＜0.01）;（3）多元逐步回归分析显示 HOMA - IR、HbA1C、BMI 是 CTRP9的独立影响因素。（4）二甲双胍治疗后 CTRP9水平显著上升（P ＜0.05）。结论 CTRP9可能在肥胖、胰岛素抵抗和 T2DM 的发生发展中起到了重要作用。二甲双胍通过升高 CTRP9水平发挥了减轻胰岛素抵抗、抗炎、抗氧化应激作用。     

血内脂素＼肿瘤坏死因子-α水平与肥胖＼2型糖尿病胰岛素抵抗及大血管病变的相关性研究

目的探讨2型糖尿病（T2DM）患者中血清内脂素、肿瘤坏死因子-α（TNF-α）水平与肥胖、胰岛素抵抗及大血管病变的关系。方法85例研究对象中43例为T2DM患者,并根据体重指数（BMI）分为T2DM肥胖组（22例）和T2DM非肥胖亚组（21例）;另有42例为糖耐量正常者,并根据BMl分为单纯肥胖组（22例）和正常对照组（20例）;各组均测定血内脂素、TNF—α、胰岛素水平、血糖和血脂、血浆高敏C反应蛋白（hs—CRP）、纤维蛋白原（Fib）、颈动脉内一中膜厚度（IMT）、BMI及腰围／臀围比值（WHR）、胰岛素抵抗指数（HOMA-IR）等指标。结果（1）4组问的内脂素、IMT、Fib、hs—CRP、胰岛素水平、HDL—C、WHR的差异均有统计学意义（P〈0．05）,进一步两两比较提示内脂素各组间的差异无统计学意义;（2）正常对照组和T2DM组内脂素和空腹胰岛素、HOMA—IR的差异均无统计学意义,两组IMT、Fib、hs—CRP的差异均有统计学意义（均P〈0,05）;（3）正常体重对照组和肥胖组内脂素和空腹胰岛素的差异均有统计学意义（P〈0．05）,而两组IMT、FIB、hs—CRP和HOMA—IR的差异均无统计学意义;（4）直线相关分析显示：内脂素与血TNF-α、腰围、腰臀比呈正相关。与IMT、Fib、hs—CRP、HOMA—IR无相关性。结论肥胖人群的血浆内脂素显著增高。内脂素不是单一而是与其它介质相互作用;内脂素与TNF—α可能相互影响,共同参与胰岛素抵抗的发生和发展。     

C反应蛋白与2型糖尿病胰岛素抵抗的关系探讨

目的：探讨C反应蛋白（CRP）与2型糖尿病（T2DM）胰岛素抵抗的关系。方法：观察2型糖尿病患者和正常对照组的血清CRP、空腹血糖（FBG）、胰岛素（FIns）、血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、糖化血红蛋白（HbA1c）水平变化及体重指数（BMI）、胰岛素抵抗指数（HOMA-IR）变化并进行比较分析。结果：T2DM患者血清CRP、HbA1c、Fins、TC、TG、FBG水平和HO-MA-IR、BMI均明显高于正常组（P〈0．05）。结论：慢性炎症炎症可能是导致T2DM和动脉粥样硬化的共同机制，是导致胰岛素抵抗、促使β细胞凋亡的重要因素，检测血清CRP可了解IR及糖尿病的发生发展。     

血清抵抗素、超敏C反应蛋白在肥胖、2型糖尿病患者中的变化

目的观察肥胖、2型糖尿病患者的血清抵抗素及超敏C反应蛋白（hs-CRP）水平及其与胰岛素抵抗的关系。方法对照组（NC）28例、糖尿病非肥胖组（DM1）30例、糖尿病肥胖组（DM2）30例。酶联免疫测定法检测空腹血清抵抗素,免疫比浊法测定血清hs-CRP。计算胰岛素分泌功能指数（HOMA2-%B）、胰岛素敏感性指数（HOMA2-%S）以及胰岛素抵抗指数（HOMA2-IR）。结果DM1组和DM2组的hs-CRP、抵抗素均高于NC组（P〈0.05）,且DM2组高于DM1组（P〈0.01）。抵抗素与体质量指数（BMI）、hs-CRP、HOMA2-%B、HOMA2-IR存在相关性（r分别为-0.252,0.563,0.225,0.667,P值均〈0.05）。hs-CRP与腰围、BMI、HOMA2-IR存在相关性（r分别为0.773,0.594,0.662,P值均〈0.05）,与抵抗素存在相关性（r为0.563,P〈0.05）。校正年龄、血糖之后,hs-CRP仍与腰围、BMI、HOMA2-IR、抵抗素独立相关。结论抵抗素水平在肥胖、2型糖尿病中升高,并与BMI、HOMA2-%B、HOMA2-%IR、hs-CRP均呈显著相关,可能为联系肥胖与胰岛素抵抗的重要因素。抵抗素、炎性因子可能在肥胖、2型糖尿病的发病中起重要作用。     

肥胖2型糖尿病患者治疗前后apelin和TNF-α水平变化及其意义

目的   探讨初诊肥胖2型糖尿病（T2DM）患者治疗前后血清Apelin与肿瘤坏死因子-α（TNF-α）水平变化及其意义。方法   收集初诊肥胖T2DM患者(T2DM组,n=61),随机接受二甲双胍（Met组,n=31）和罗格列酮(Rog组,n=30)治疗,健康体检者为正常对照组（NC组,n=30）。检测治疗前后血清Apelin、TNF-α、空腹血糖（FBG）、空腹胰岛素（FIN）、糖化血红蛋白（HbA1c）水平及体质量指数（BMI）的变化,以稳态模型计算胰岛素抵抗指数（HOMA-IR）。结果   肥胖T2DM患者Apelin、TNF-α水平均显著高于NC组（P均＜0.05）,且与BMI、FIN、FBG、HOMA IR均呈正相关（P均＜0.05）;治疗后Apelin、TNF α、FBG、HbA1c水平均显著下降（P均＜0.05）, 其中Rog组Apelin、TNF-α水平较Met组下降更为显著（P＜0.05）,Met组BMI显著下降（P＜0.05）,Rog组无显著变化（P＞0.05）;相关分析显示治疗前后Apelin与TNF α均密切相关（P均＜0.05）。结论    肥胖T2DM患者血清高水平Apelin、TNF-α可能参与胰岛素抵抗的发生发展过程。     

腹腔镜可调控性胃束带术治疗肥胖伴T2DM的相关机制

目的探讨腹腔镜可调控性胃束带（LAGB）治疗2型糖尿病（T2DM）的相关机制。方法肥胖伴T2DM患者20例、单纯性肥胖患者20例行LAGB手术。检测术前、术后第1、3、6、12个月空腹血清Leptin水平,空腹血清胰岛素（FINS）、C肽、糖化血红蛋白（HbAlc）水平,空腹血糖（FBG）水平及体质量并计算体质量指数（BMI）。结果40例受试者LAGB术后6个月和12个月BMI由术前平均（38±5）kg／m2分别降至（30±4）kg／m2和（28±7）kg／m2（P〈0．05）。术前糖尿病组FBG、FINS、胰岛素抵抗指数[HOMA—IR=FINS（mU／L）×FBG（mmol／L）／22．5]、HbAlc和C肽水平明显高于对照组（P〈0．01）,术后6个月、12个月FBG由术前（8．5±3．0）mmol／L分别降至（6．5±1．0）mmol／L和（5．9±0．5）mmol／L,FINS和HbAlc术后3个月后降至正常范围。两组患者术后12个月内血清Leptin指标逐渐下降,糖尿病组血清Leptin与FBG、FINS、HbAlc、C肽及HOMA—IR呈正相关。结论LAGB能有效治疗肥胖症及T2DM,通过降低血清Leptin水平,减轻瘦素抵抗,进而减轻胰岛素抵抗,达到降低血糖的目的。     

2型糖尿病与脂代谢异常的相关性研究

目的探讨新诊断2型糖尿病（diabetes mellitus,DM）患者体重指数（body mass index,BMI）与胰岛素抵抗（insulin resistance,IR）及血脂的关系。方法128名新诊断2型DM（T2DM）根据体重指数分为三组（肥胖组、超重组、体重正常组、）,测定三组受试者血糖、胰岛素及血脂水平,用HOMA模型公式计算胰岛素抵抗指数（HOMA—IR）。结果肥胖组HOMA—IR明显高于超重组及体重正常组（P〈0．001）,而超重组HOMA—IR亦高于体重正常的DM组（P〈0．01）;甘油三酯（TG）水平在肥胖组明显高于其他两组,高密度脂蛋白（HDL）则低于其他两组;随着BMI升高,血压有增高趋势。结论初诊肥胖及超重2型DM患者与胰岛素抵抗、血脂异常、高血压相关。     

2型糖尿病患者颈动脉内膜中层厚度与胰岛素抵抗关系研究

目的探讨2型糖尿病患者颈动脉内膜中层厚度与胰岛素抵抗的关系。方法根据糖化血红蛋白（HbA1C）的水平把糖尿病患者分成DM1组和DM2组,与正常对照组颈动脉内膜中层厚度（IMT）、空腹血糖（FBG）、HbA1C、空腹血清胰岛素（FINS）、胰岛素抵抗（HOMA IR）指数进行对照。结果2型糖尿病患者的颈动脉IMT值与FBG、BM I（体重指数）、HbA1C和HOMA IR指数呈显著正相关。结论2型糖尿病患者的颈动脉IMT值与糖尿病患者的血糖控制程度和胰岛素敏感性密切相关。     

炎症因子在2型糖尿病大血管病变患者中意义及其相关性

目的：探讨炎症因子白细胞介素－8（IL－8）、肿瘤坏死因子－α（TNF－α）及C－反应蛋白（CRP）与2型糖尿病（ T2DM）大血管病变的关系。方法选取2012年6月至2014年6月本院收治的124例T2DM患者为研究对象,根据彩色多普勒超声诊断结果将患者分为T2DM无大血管病变组（ G1组）68例及合并大血管病变组（ G2组）56例。另选取同期行身体检查的60例正常体检者为对照组,应用放射免疫法测定三组CRP水平,应用酶联免疫法测定三组IL－8、TNF－α水平,分析CRP、IL－8、TNF－α与空腹胰岛素（FINS）、空腹血糖（FBG）、同型半胱氨酸（Hcy）、甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白（LDL－C）、高密度脂蛋白（ HDL－C）、同型半胱氨酸（Hcy）、体重指数（ BMI）、腰臀比（ WHR）的关系。结果 G2组血清CRP、IL－8、TNF－α水平、FINS、HOMA－IR、冠心病史、脑卒中史高于G1组（ P＜0.05）,G1组与G2组血清CRP、IL－8、TNF－α、FINS、FBG、Hcy、TG、TC、LDL－C、WHR水平、高血压史、冠心病史、脑卒中史显著高于对照组（P＜0.05）。经Pearson相关因素分析可知,CRP与IL－8、 TNF－α、FINS、HOMA－IR呈正相关（P＜0.05）。 IL－8与TNF－α、FINS呈正相关（P＜0.05）。 TNF－α与FINS、HOMA－IR呈正相关（P＜0.05）。经Logistic多因素回归分析可知,CRP、IL－8、TNF－α、FINS、HOMA－IR是导致T2DM大血管病变的独立危险因素。结论 CRP、IL－8、TNF－α可能是T2DM合并大血管病变的危险因子,三者可能通过胰岛素抵抗及相关作用参与T2DM大血管病变的发生及进展过程。     

非肥胖2型糖尿病胰岛素抵抗与脂代谢的关系

目的：研究非肥胖2型糖尿病患者胰岛素抵抗与血脂代谢的关系。方法：测定72例非肥胖糖尿病患者及60例非肥胖正常人空腹血糖（FPG）、糖化血红蛋白（HbA1c)、空腹胰岛素（FINS）、体重指数（BMI）及脂代谢指标，并进行相关性分析。结果：（1）72例非肥胖糖尿病患者FPG、HbA1c、FINS、胆固醇（CHO）、甘油三酯（TG）、低密度脂蛋白（LDL）、极低密度脂蛋白（VLDL）及胰岛素抵抗指数（HOMA－IR）明显高于正常对照组。（2）Pearson相关分析表明HOMA－IR与CHO、TG、LDL呈正相关。（3）逐步回归分析发现以HOMA－IR为因变量，CHO、TG、LDL为自变量，TG对HOMA－IR有影响。结论：高TG是非肥胖2型糖尿病胰岛素抵抗的特点，TG升高可作为非肥胖2型糖尿病胰岛素抵抗的独立因素。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.