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1.
Burov YV Uzdenskii AB Robakidze TN 《Bulletin of experimental biology and medicine》2000,129(4):362-364
The effect of new agent Vp (9-butylamine-3,3-dimethyl-3,4-dihydroacridine-1(2H) hydrochloride) on lifetime of isolated mechanoreceptive
crayfish neurons was evaluated by the duration of its impulse activity. Vp significantly and dose-dependently prolonged the
lifetime of both spontaneously degrading neurons and neurons degrading under conditions of inhibition of various stages of
the energy metabolism: glycolysis and oxidative phosphorylation. The effect of Vp in a concentration of 10−7 M surpassed that of amiridine. Piracetam also prolonged the lifetime of spontaneously degrading neurons, but only in very
high concentration (10−2 M). It is concluded that Vp possesses a neuroprotective activity.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 129, No. 4, pp. 430–433, April, 2000 相似文献
2.
A study is performed of the effect of the phenol antioxidant katavidan on autooxidation of microsomes from rat liver exposed
to visible light. It is shown that katavidan in a concentration of 10−3 M inhibits but in concentrations of 10−5–10−7 M stimulates autooxidation of microsomes. No stimulation is observed under conditions of dark incubation.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, № 10, pp. 393–394, October, 1994
Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences 相似文献
3.
N. G. Bogdanova T. V. Lelekova N. P. Pal'mina 《Bulletin of experimental biology and medicine》2000,129(1):30-32
Effects of the regulatory peptide thyroliberin on microviscosity of lipid components of endoplasmic reticulum biological membranes
in mouse hepatocytes were studied by electron paramagnetic resonance. Thyroliberin in a concentration of 10−3–10−18 M decreased microviscosity of surface layers of membrane lipids. This decrease was the most pronounced (30%) under effects
of 10−10 and 10−16 M thyroliberin. Physiological effects of thyroliberin corresponded to its influence on the membrane structure.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 129, No. 1, pp. 38–40, January, 2000 相似文献
4.
T. G. Pukhal’skaya O. A. Kolosova M. Y. Men’shikov A. M. Vein 《Bulletin of experimental biology and medicine》2000,130(1):633-635
Flunarizine and cinnarizine (IC50 6.8×10−6 and 2.8×10−5 M, respectively) inhibited3H-serotonin uptake by platelets. In higher doses, they blocked serotonin-induced platelet aggregation and stimulated3H-serotonin release from these cells. Imipramine did not affect serotonin-releasing effects of preparations. In all patients
cinnarizine was more potent in inhibiting serotonin uptake, and in half of the patients cinnarizine displayed higher activity
as an inductor of serotonin release.
Translated fromByulleten's Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 7, pp. 24–27, July, 2000 相似文献
5.
T. S. Golubeva O. Yu. Chertov M. V. Kiselevskii A. R. Tuguz 《Bulletin of experimental biology and medicine》1997,124(5):1104-1106
No nucleosomal fragmentation of DNA from lung adenocarcinoma cells was observed after 18 h of their incubation with normal
human platelets isolated from human peripheral blood. Platelet activation with 10−6, 10−7, and 10−8 M PAF did not lead to nucleosomal fragmentation of the target cell DNA.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 11, pp. 547–549, November, 1997 相似文献
6.
Pukhal'skaya TG Kolosova OA Men'shikov MY Vein AM 《Bulletin of experimental biology and medicine》2000,130(7):633-635
Flunarizine and cinnarizine (IC50 6.8×10−6 and 2.8×10−5 M, respectively) inhibited3H-serotonin uptake by platelets. In higher doses, they blocked serotonin-induced platelet aggregation and stimulated3H-serotonin release from these cells. Imipramine did not affect serotonin-releasing effects of preparations. In all patients
cinnarizine was more potent in inhibiting serotonin uptake, and in half of the patients cinnarizine displayed higher activity
as an inductor of serotonin release.
Translated fromByulleten's Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 7, pp. 24–27, July, 2000 相似文献
7.
A. V. Semeikin V. M. Rzheznikov O. N. Minailova Ya. V. Dobrynin E. E. Mayatskaya 《Bulletin of experimental biology and medicine》1999,127(3):272-274
Antiproliferative effect of bis-β-chlorethylamine estrogen derivatives on human ovarian adenocarcinoma CaOV cells depends
on the dose of the drug. In concentrations of 10−5 and 5×10−6 M they inhibit, while in a dose of 5×10−7 M stimulate cell proliferation. It is assumed that CaOV cells express estradiol receptors. The interaction of these cytostatics
with estrogen-binding sites on CaOV cells is demonstrated.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 127, No. 3, pp. 302–304, March, 1999 相似文献
8.
E. I. Solntseva Yu. V. Bukanova R. U. Ostrovskaya T. A. Gudasheva T. A. Voronina V. G. Skrebitskii 《Bulletin of experimental biology and medicine》1996,121(2):138-142
The effect of two nootropics, piracetam and N-phenylacetyl-L-prolyglycine ethyl ester (GVS-111), is studied by measuring high-threshold
K+ and Ca2+ currents in isolated snail neurons using a two-microelectrode patch-clamp technique. Piracetam and GVS-111 are shown to reduce
the amplitude of both the K+ and the Ca2+ (to a lesser extent) current. The threshold concentrations for GVS-111 and piracetam are 10−9-10−8 M and 1–5×10−4 M, respectively. It is assumed that the antiamnestic effect of the nootropics is partially mediated by a blockade of ion
channels of the neuronal membrane.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N
o
2, pp. 151–155, February, 1996
Presented by G. N. Kryzhanovskii, Member of the Russian Academy of Medical Sciences 相似文献
9.
Antioxidant properties of thiamine 总被引:1,自引:0,他引:1
P. I. Lukienko N. G. Mel'nichenko I. V. Zverinskii S. V. Zabrodskaya 《Bulletin of experimental biology and medicine》2000,130(3):874-876
Thiamine (10−4–10−6 M) inhibits lipid peroxidation in rat liver microsome and free radical oxidation of oleic acidin vitro. Thiamine interacts with free radicals and hydroperoxides and is oxidized to thiochrome and thiamine disulfide. The antioxidant
effect of thiamine is probably related to sucessive transfer of 2H+ from the NH2 group of the pyrimidine ring and H+ from the thiazole ring (after its opening) to reactive substrates.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 9, pp. 303–305, September, 2000 相似文献
10.
V. M. Smirnov 《Bulletin of experimental biology and medicine》1997,124(1):635-638
The mechanism by which heart rate is increased upon stimulation of vagal centers is studied using frog heart preparations
perfused with Ringer—Locke solution containing atropine and/or benzohexonium. Atropine stimulates vagus-induced heart-rate
acceleration in dilutions of 10−6 and 10−5 g/ml. In a dilution of 10−4 g/ml both atropine and benzohexonium abolish vagal tachycardia. Rausedyl (3–4 injections, 5 mg/kg, at 18–20-h interval) prevents
tachycardia. Stimulation of both halves of the medulla oblongata increases heart rate to a greater extent than stimulation
of one half.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 7, pp. 16–20, July, 1997 相似文献
11.
Lukienko PI Mel'nichenko NG Zverinskii IV Zabrodskaya SV 《Bulletin of experimental biology and medicine》2000,130(9):874-876
Thiamine (10−4–10−6 M) inhibits lipid peroxidation in rat liver microsome and free radical oxidation of oleic acidin vitro. Thiamine interacts with free radicals and hydroperoxides and is oxidized to thiochrome and thiamine disulfide. The antioxidant
effect of thiamine is probably related to sucessive transfer of 2H+ from the NH2 group of the pyrimidine ring and H+ from the thiazole ring (after its opening) to reactive substrates.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 9, pp. 303–305, September, 2000 相似文献
12.
Semeikin AV Rzheznikov VM Mayatskaya EE Smirnova ZS 《Bulletin of experimental biology and medicine》2000,129(6):592-594
The effects of four new synthetic bis-β-chloroethylamine-containing estrogens and known cytostatic agents chlorophenacyl and
estradiol mustard were compared on monolayer cultures of transformed L-929 fibroblasts (from murine skin sarcoma). The drugs
within the concentration range of 10−5-5×10−7M inhibited proliferation of cultured cells by 67%. Chlorophenacyl displayed the least antiproliferative activity (15% inhibition
at 10−5M). Steroid nucleus introduced into the molecule enhanced antiproliferative activity of test drug in comparison with chlorophenacyl,
probably due to accumulation of the hormone-cytostatic molecules in cells. Estradiol had no effect on proliferative activity
of L-929 cells, and no specific estrogen-binding sites were found in cultured transformed fibroblasts. The antiproliferative
effect of hormone-cytostatics on this culture is not mediated via specific interactions with estrogen receptors.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 129, No. 6, pp. 695–697, June, 2000 相似文献
13.
P. V. Sergeev S. A. Chukaev Yu. A. Korovkina 《Bulletin of experimental biology and medicine》1997,124(2):786-788
Effects of estradiol and testosterone and of the antiandrogens cyproterone acetate, niftolide, and antiestrogen tamoxifen
on the activities of human erythrocyte glutathione peroxidase and glutathione reductase were studiedin vitro. In contrast to hormone preperations, antihormones in high concentrations (10−4−5×10−4 M) modified the enzyme activities. Cyproterone acetate and tamoxifen increased the activity of glutathione reductase, while
tamoxifen stimulated glutathione reductase and inhibited glutathione peroxidase. Niftolide inhibited both enzymes.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 8, pp. 185–187, August, 1997 相似文献
14.
A. A. Karelin R. N. Korotkina G. N. Matskevich V. V. Borisov A. A. Vishnevskii L. V. Polikarpova A. G. Kunitsyn 《Bulletin of experimental biology and medicine》2000,130(4):973-975
In vitro effects of folic acid (10−5, 10−4, and 10−3 M) on activities of γ-glutamyltransferase and glutathione reductase, the enzymes involved in glutathione metabolism, were
studied in tissue samples obtained after surgical treatment of the lungs and thymus. Folic acid did not change γ-glutamyltransferase
activity in lung cancer tissue, but in thymoma tissue this substance in a concentration of 10−3 M inhibited it by 16%. Folic acid had no effects on glutathione reductase activity in benign tumors and normal lung and thymus
tissues, but increased this activity in thymoma and lung cancer tissues. Activation of glutathione reductase was probably
related to binding of folic acid in the allosteric center of the enzyme, which probably induced conformational changes in
the catalytic center, acceleration of electron transport from NADPH2 to oxidized glutathione via flavin adenine nucleotide, and intense production of reduced glutathione.
Translated fromByulleten', Eksperimental'noi Biologii i Meditsiny, Vol. 130, No. 10, pp. 422–425, October, 2000 相似文献
15.
T. V. Davydova V. A. Evseev V. G. Fomina L. A. Basharova O. I. Mikovskaya K. L. Pletsityi 《Bulletin of experimental biology and medicine》1998,126(3):934-936
Experiments on C57B1/6 mice prone to experimental alcoholism demonstrated that adoptive transfer of splenocytes from animals
with the abstinent syndrome stimulates alcohol consumption. Incubation of splenocytes with antibodies to serotonin in a dose
of 10−7 M for 1 or 24 h completely suppressed this effect.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 9, pp. 328–330, September, 1998 相似文献
16.
Synthesis and properties of the immunotoxin CD5-ricin 总被引:1,自引:0,他引:1
G. V. Vikha L. D. Uzhinova A. Zh. Kashaeva V. V. Goshev A. V. Krylov A. Yu. Baryshnikov R. G. Vasilov 《Bulletin of experimental biology and medicine》1995,119(1):70-73
Synthesis and properties of an immunotoxin produced by conjugating ricin with a novel monoclonal antibody (IgG3 of the ICO-104
class) are described. Cytolytic activity of the synthesized immunotoxin, determined by two independent methods and expressed
in LD50, is 0.3–0.6×10−7 M. Its specificity for target cells containing the CD5 antigen is shown.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
o
1, pp. 76–79, January, 1995
(Presented by Yu. A. Romanov, Member of the Russian Academy of Medical Sciences) 相似文献
17.
N. F. Avrova V. P. Ivanova V. A. Tyurin I. A. Gamalei I. V. Klyubin I. A. Shchepetkin E. V. Borunov Yu. Yu. Tyurina 《Bulletin of experimental biology and medicine》1994,117(1):45-47
It is shown that ganglioside GM1 in picomolar concentrations stimulates the phorbol-12-myristate-13-acetate (PMA)-induced
generation of active forms of oxygen by neutrophils and peritoneal macrophages. GM1 (10−11 M) is found to enhance the luminol-dependent chemiluminescence induced by 10−8 M PMA in mouse macrophages in comparison with the effect of PMA alone.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N
o
1, pp. 44–46, January, 1994
Presented by A. N. Klimov, Member of the Russian Academy of Medical Sciences 相似文献
18.
I. P. Ashmarin V. E. Pastorova L. A. Lyapina 《Bulletin of experimental biology and medicine》1998,125(5):437-439
Elementary proline-containing peptides being added to rat platelet-rich plasma or platelet suspension in concentrations of
10−1–10−7 mg/ml elicit a considerable antiplatelet effect. Efficiency of inhibition of platelet aggregation increases in the following
order: Pro-Gly>Pro-Gly-Pro>Gly-Pro-Gly-Gly. Intravenous injection of these peptides activates blood anticoagulation system
in experimental animals.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 125, No. 4, pp. 496–499, May, 1998 相似文献
19.
G. V. Cherepnev Yu. D. Slabnov A. P. Tsibul'kin F. G. Karimova R. S. Garaev 《Bulletin of experimental biology and medicine》1999,127(1):59-62
High radioprotective activity of xymedon was shown in mice. Radioprotective effects of this preparation were accompanied by
restoration of the delayed-type hypersensitivity response and Ca2+-ATPase activity in splenocytes which were inhibited by γ-irradiation (3 Gy). At concentrations of 10−3 and 10−6 M xymedon stimulated the activity of DNA topoisomerase-I of splenocyte nuclei. Here we discuss a mechanism of radioprotective
effects of pyrimidine derivatives associated with the inhibition of apoptosis of lymphoid cells and the stimulation of proliferation
and differentiation of lymphoid precursor cells.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 127, No. 1, pp. 66–70, January, 1999 相似文献
20.
I. V. Kubasov I. I. Krivoi E. V. Lopatina 《Bulletin of experimental biology and medicine》1994,118(5):1153-1155
Nerve stimulation is performed in rat phrenicodiaphragmal preparations with armine-inhibited acetylcholinesterase. Acetylcholine
(1×10−7 M) is added to the saline for 15 min, and as it is washed off (during 1–2 h), the amplitude of isometric contractions and
of the total action potential increases in the continuously stimulated muscle. Contractions in response to direct muscle stimulation
remain unchanged. The membrane resting potential of muscle fibers exposed to acetylcholine shifts by 2–3 mV toward hyperpolarization
and remains at this level for 2 h after the removal of acetylcholine from the saline.
Presented by S. N. Golikov, Member of the Russian Academy of Medical Sciences
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
o
11, pp. 457–459, November, 1994 相似文献