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1.
Control of CD4 T cell fate by antigen re-stimulation with or without CTLA-4 engagement 24 h after priming 总被引:2,自引:0,他引:2
After two consecutive inoculations with Staphylococcus enterotoxin B (SEB) at 24 h intervals in vivo, CD4 T cells became anergic to the antigen challenge in vitro. Administration of anti-CTLA-4 mAb in conjunction with the second SEB inoculation 24 h after antigen priming interfered with anergy and CD4 T cells became T(h)2 cells. However, the anergy induction was not ablated when SEB and anti-CTLA-4 mAb were administered 48 or 72 h after antigen priming. Moreover, anti-CTLA-4 mAb without SEB did not interfere with anergy nor promoted the T(h)2 differentiation. T-antigen-presenting cell (APC) interaction in vitro in the presence of high doses of antigen and anti-CTLA-4 mAb induced a T(h)2-polarizing cytokine IL-6 and IL-10. IL-10 then down-modulated a T(h)1-polarizing cytokine IL-12. The results demonstrate that 24 h after the initial antigen stimulation, CD4 T cells enter the critical activation phase where antigen re-stimulation with or without CTLA-4 engagement alters the fate of the cell, anergy or differentiation respectively. Once anergy is interfered with, T(h)2-polarizing cytokines produced upon prolonged T-APC interaction favor the T(h)2 differentiation. 相似文献
2.
To investigate whether a clonal deletion mechanism is responsible for the mature T cell tolerance that may be induced in vivo by TCR signal to anti-CD4 (H129.19 mAb) coated cells, we analyzed the T cell repertoire in anti-CD4 mAb treated BALB/c mice by flow cytometry following TCR signals through anti-alpha beta TCR mAb or SEB superantigen. Lymph nodes showed a strong reduction in the CD4+/CD8+ cell ratio, and a selective clonal loss of CD4+ V beta 8+ cells 4d following anti-alpha beta TCR or SEB injection, respectively. Following lymph node cell activation in a short-term in vitro assay with SEB or anti-V beta 8 mAb, a selective elimination of CD4+ V beta 8+ cells was again detected, and DNA fragmentation analysis disclosed a cell death by apoptosis. These findings suggest that TCR triggering transduces an apoptotic signal into CD4+ mAb saturated cells that in turn leads to specific holes in the mature T cell repertoire. 相似文献
3.
Immobilized anti-CD3 mAb induces anergy in murine naive and memory CD4+ T cells in vitro 总被引:1,自引:0,他引:1
The induction of non-responsiveness in resting murine CD4+ T cells was
investigated using immobilized anti-CD3 mAb. Incubation of freshly isolated
CD4+ T cells with immobilized anti-CD3 mAb led to apoptosis in 40-60%
cells. The surviving cells were profoundly non-responsive to subsequent
mitogenic stimulation. The non-responsive state was characterized by a lack
of IL-2 production and hyper-responsiveness to added IL-2, but was not
explained by further activation-induced cell death. The induction of
non-responsiveness was not due to modulation of the TCR-CD3 complex, and
required partial activation of the T cells in that it was accompanied by an
increase in cell size and was inhibited by addition of cyclosporin A.
Finally, analysis of anti-CD3-mediated responses in naive and memory CD4+ T
cells, separated on the basis of CD44 expression, showed that both naive
and memory T cells have similar sensitivity to immobilized anti-CD3
mAb-induced activation, apoptosis and anergy. These results demonstrate
that TCR-CD3 engagement on freshly isolated resting CD4+ naive and memory T
cells, In the absence of co-stimulation, as achieved by plastic-immobilized
anti-CD3 mAb, induces both anergy and cell death.
相似文献
4.
S W Van Gool J Vermeiren K Rafiq K Lorr M de Boer J L Ceuppens 《European journal of immunology》1999,29(8):2367-2375
Although CD28 triggering provides an important co-stimulatory signal to T cells, blocking the CD80/CD86 - CD28 interaction with CTLA-4lg fusion protein is not sufficient for tolerance induction in vivo or in vitro. According to more recent data, interruption of the CD40 - CD154 interaction might complement the effect of CTLA-4lg and induce graft acceptance. We studied the effects of a blocking anti-CD40 monoclonal antibody (mAb) and/or blocking anti-CD80/anti-CD86 mAb in cultures of human peripheral blood mononuclear cells (PBMC) stimulated with allogeneic PBMC. T cells activated by alloantigens in the presence of anti-CD80, anti-CD86 and anti-CD40 entered a state of alloantigen-specific non-responsiveness as evidenced upon restimulation by lack of proliferation, cytotoxic activity, and IL-2, IL-5 and IL-13 production. IFN-gamma production during restimulation was less than in the control cultures, while the production of IL-10 was enhanced. Addition of recombinant IL-2 during the restimulation rescued alloantigen-specific activity. We conclude that the simultaneous blocking of the CD40 - CD154 and CD80/CD86 - CD28 interaction during allogeneic T cell activation induces T cell anergy. Since anergic cells induced by this treatment still produce high levels of IL-10, the latter could contribute to modulation of antigen-presenting cell activity and to bystander suppression of residual reactive T cells. 相似文献
5.
Primary murine CD4+ and CD8+ T helper (Th) cells provide help for various immune responses by secreting lymphokines which activate effector cells. The purpose of the present study was to investigate the co-stimulatory signals that, together with T cell receptor (TCR) cross-linking, induce phenotypically distinct primary Th cells to secrete IL-2 and proliferate. We isolated highly purified populations of primary CD4+ or CD8+ T cells and stimulated them in vitro with platebound anti-CD3 mAb. TCR cross-linking by anti-CD3 mAb induced both IL-2 receptor expression and responsiveness to exogenous IL-2, but was not sufficient to induce either IL-2 secretion or T cell proliferation. Rather, for both CD4+ and CD8+ primary Th cells, IL-2 secretion and proliferation required both TCR cross-linking and antigen presenting cell (APC)-derived co-stimulatory signals. Based on G-10 adherence and sensitivity to gamma-irradiation, the APC populations able to induce primary CD4+ Th cells and primary CD8+ Th cells to secrete IL-2 were indistinguishable. In addition, we found that either IL-1 or IL-6 could replace the requirement for APC-derived co-stimulatory signals for IL-2 secretion and proliferation by both primary CD4+ Th cells and primary CD8+ Th cells. Thus, the present study has examined and compared the co-stimulatory requirements of rigorously purified subsets of IL-2-secreting primary CD4+ and primary CD8+ T cells.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
6.
The negative role exerted by cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) in the regulation of T-cell activity, as induced by T-cell receptor (TCR)/CD3 and CD28 costimulation, has been widely described. In the present work we investigated the role of CTLA-4 in the control of cell activation, as induced by costimulation of the adhesion molecule lymphocyte function-associated antigen-1 (LFA-1) in murine CD4+ T cells. Results show that CTLA-4 engagement inhibits interleukin-2 (IL-2) production, not only when induced by CD3/CD28 costimulation, but also when CD4+ T cells are costimulated by anti-CD3 and anti-LFA-1 monoclonal antibodies (mAbs). LFA-1 has been described to induce Ca2+ mobilization also in the absence of TCR engagement. Moreover, we found that CTLA-4 engagement negatively affects Ca2+ mobilization and NF-AT activation, as induced by LFA-1 engagement alone. PLCgamma1 phosphorylation was also dampened within minutes after CTLA-4 engagement. Altogether these data indicate that through the control of signals induced by different receptors, CTLA-4 could be a global attenuator of T-cell activation. 相似文献
7.
It is well established that peripheral CD8+ and CD4+ T cells display different requirements for in vitro activation by mitogenic mAb. Most CD4+ T cells can be activated by anti-CD3 or mitogenic combinations of anti-CD2. In contrast, CD8+ T cells display minimal responses to CD3 activation, and no proliferation is observed via CD2 activation. Purified peripheral blood CD8+ T cells, stringently depleted of APC, have been studied for their capacity to respond to mAb directed against CD3, CD2 and CD28, used alone or in combination. It is demonstrated that proliferation can be induced by co-stimulation of CD2 and CD28. This does not require autologous APC. CD8+ T cells can also be activated by the combination of anti-CD3 plus anti-CD28 in the presence of APC, but only minimal cell proliferation is obtained in the absence of APC. The response via CD2 plus CD28 is IL-2-dependent, as demonstrated by the ability of mAb against the IL-2 receptor to block proliferation, and is almost completely inhibited by cyclosporine A (CsA). These results suggest that the signal generated by stimulation of CD28 in combination with CD2 differs from that seen with CD28 activation combined with either PMA or CD3. Induction of IL-2 gene activation in CD8+, CD28+ peripheral T cells may therefore require additional "second signals", which are not necessary for activation of CD4+ cells. One such signal might be the interaction between CD28 and its natural ligand. 相似文献
8.
Increasing evidence in both murine and human systems suggests that the interaction of the T cell surface antigens CD28/CTLA4 with their ligand B7 on the antigen-presenting cells (APC) is the critical costimulatory pathway involved in the induction of maximal T cell activation and the prevention of induction of anergy. It has also been demonstrated that efficient induction of clonal expansion of normal CD4+ T cells requires the delivery of the T cell receptor (TCR) ligand and costimulation by the same APC. We demonstrate here that normal murine CD4+ T cells can be efficiently activated by soluble anti-CD3 cross-linked by fixed macrophages and by a costimulatory signal delivered by a bystander APC, B7-transfected L cells. The major factor which determined the ability of an APC to provide costimulation in “trans” was the level of cell surface B7 expression. The requirement for B7 costimulation appears to be at initial stage of TCR engagement since optimal T cell activation was only observed when TCR triggering and B7 costimulatory activity were delivered at same time by different APC. Induction of maximal proliferation of both naive CD45RBhi and memory CD45RBlo CD4+ T cells was B7 dependent and both populations of cells responded equally well to the B7 costimulation delivered in “trans”. Furthermore, trans-costimulation provided by B7 transfected L cells efficiently prevented the induction of anergy in normal murine CD4+ T cells induced by anti-CD3 cross-linked by fixed-resting macrophages. Addition of exogenous interleukin-2 (IL-2) and IL-7 to the primary culture in the absence of B7-transfected L cells or addition of IL-2 to the culture containing the B7 transfectant and CTLA4Ig completely prevented the induction of hyporesponsiveness. These findings raise the possibility that in certain pathological states, CD4+ T cells in vivo may be activated by costimulation delivered by bystander APC. 相似文献
9.
Mary K. Kennedy Kathleen S. Picha William C. Fanslow Kenneth H. Grabstein Mark R. Alderson Ky N. Clifford Wilson A. Chin Kendall M. Mohler 《European journal of immunology》1996,26(2):370-378
We have previously shown that T cell receptor-activated mouse T helper (Th)1 clones induce the production of interleukin (IL)-12 by splenic antigen-presenting cells (APC). Here, we show that the expression of CD40L by activated T cells is critical for T cell-dependent IL-12 production by mouse macrophages. IL-12 was produced in cultures containing alloreactive Th1 clones stimulated with allogeneic peritoneal macrophages, or in cultures of splenocytes stimulated with anti-CD3. Anti-CD40L monoclonal antibodies (mAb) inhibited the production of IL-12, but not IL-2, in these cultures by ?90% and had dramatic inhibitory effects on antigen-dependent proliferation of Th1 clones. In addition, both activated T cells and a Th1 clone derived from CD40L knockout mice failed to induce IL-12 production from splenic APC or peritoneal macrophages. Finally, macrophages cultured in the absence of T cells produced IL-12 upon stimulation with soluble recombinant CD40L in combination with either supernatants from activated Th1 clones or with interferon-γ and granulocyte/macrophage colony-stimulating factor. Thus, both CD40L-dependent and cytokine-mediated signals from activated T cells are required to induce the production of IL-12 by macrophages. A blockade at the level of IL-12 production may explain, at least in part, the dramatic ability of anti-CD40L mAb to inhibit disease in animal models that are dependent upon the generation of a cell-mediated immune response. Moreover, a defect in T cell-dependent induction of IL-12 may contribute to the immune status of humans that lack functional CD40L. 相似文献
10.
B7-CD28 interaction is a late acting co-stimulatory signal for human T cell responses 总被引:1,自引:0,他引:1
Zhang YQ; Joost van Neerven RJ; Van Gool SW; Coorevits L; de Boer M; Ceuppens JL 《International immunology》1997,9(8):1095-1102
The interaction of CD28 with one of the B7 molecules (CD80 and CD86) on
professional antigen-presenting cells (APC) is generally considered as the
most important co-stimulatory signal for T cell activation. APC in a
resting condition express either no or only low levels of B7 molecules.
These are up-regulated as a result of interactions with activated T cells,
thus suggesting that B7-CD28 interaction is not required at initiation of T
cell activation. To study this issue, we blocked B7-CD28 interaction at
various time points after in vitro stimulation of peripheral blood T cells
with allogeneic monocytes. Epstein-Barr virus-transformed B cells or
soluble antigens. We observed that T cell proliferation and IL-2 production
were inhibited by B7- blocking agents (CTLA-4-Ig or anti-B7 mAb) almost to
the same degree when added either at initiation of culture or 24 h later.
B7-blocking agents still resulted in significant inhibition of allogeneic T
cell activation when added after 48 h. Furthermore, when CTLA-4-Ig was
added at the start of an allogeneic T cell stimulation, addition of
anti-CD28 mAb after 24 h of culture nearly fully restored T cell
proliferation to control levels. Finally, we demonstrate that delayed
addition of B7- blocking agents together with cyclosporin A 1 day after the
onset of culture of T cells with allogeneic B cells is highly efficient to
induce energy as evaluated by lack of proliferation, cytotoxic T lymphocyte
reactivity and IFN-gamma or IL-5 production upon alloantigen rechallenge.
Taken together, our data can explain why B7 expression on APC is not
required at the time of initial APC-T cell contact, and suggest that the
effect of the CD28 signal indeed consists in prolonging IL-2 production and
amplifying T cell responses, rather than in providing a critical
co-stimulatory signal at the time of initial TCR triggering.
相似文献
11.
In this study we investigated the differential effect of the co-stimulatory receptor ligand molecules CD2/LFA-3, LFA-1/ICAM-1, and CD28/B7 on microbial superantigen mediated activation of CD4+ T cells. Highly purified CD4+ T cells, depleted of antigen presenting cells (APCs), do not proliferate in response to the superantigen, staphylococcal enterotoxin B (SEB). However, CD4+ T cells do respond to SEB in the presence of the LFA-3, ICAM-1, and B7 positive erythroleukemic cell line K562, murine L cells, human B7 transfected L cells or CD28 mAb. The K562 plus SEB induced response can be inhibited by combinations of mAbs to CD2 and LFA-1, and to LFA-3, ICAM-1, and B7. Addition of CD28 mAb to the CD2 and LFA-1 inhibited cultures could restore the response. Furthermore, soluble CD28 mAb alone is able to synergize with SEB to induce a proliferative CD4+ T cell response. CD4+ T cells depleted of APCs could also be activated by a pool of four mAbs directed to the V beta 5, V beta 6, V beta 8, and V beta 12 region of the TCR when a co-stimulatory signal was provided by the CD28 mAb, while the V beta mAbs alone or in combination are unable to activate CD4+ T cells in the absence of APCs. In contrast, addition of soluble mAbs to CD2 and LFA-1 molecules failed to co-stimulate SEB activated CD4+ T lymphocytes. The kinetics of the different modes of activation are distinct. SEB induced proliferation is most efficient in the presence of autologous APCs with maximal proliferation at a log4 lower SEB concentration than when CD28 mAbs were used. SEB plus K562 activation peaks on day 7, while SEB plus CD28 mAb induced proliferative responses do not peak until day 9. Thus, superantigen mediated activation of CD4+ T cells requires co-stimulatory signals, among which CD28 has distinct and unique effects. 相似文献
12.
Moro M Filippi C Gallard A Malherbe L Foucras G Akiba H Yagita H Guéry JC Glaichenhaus N 《European journal of immunology》2002,32(12):3566-3575
The interactions between CD28 and its ligand CD86 are critical for the regulation of T cell responses. However, it is not clear whether CD4+ T cells expressing low and high avidity TCR are equally dependent on CD28 costimulation for their activation and expansion. To address this issue, we have used multimers of I-Ad molecules linked to a peptide derived from the Leishmania major homolog for the receptor of activated C kinase (LACK) antigen to compare the fate of LACK-specific CD4+ T cells in Leishmania-infected BALB/c mice which have been treated or not with anti-CD86 mAb. Although the administration of anti-CD86 mAb did not completely prevent the expansion of LACK-specific T cells, their frequency and number were markedly reduced. In mice treated with anti-CD86 mAb as well as in control animals, L. major induced the clonal expansion of LACK-specific T cells which expressed a canonical low avidity Valpha8/Vbeta4 TCR. Taken together, our results suggest that the molecular interactions between CD28 on T cells and CD86 on APC serve to amplify and modulate T cell responses without promoting breadth in the TCR repertoire. 相似文献
13.
《Immunological investigations》2013,42(6):965-976
Conjugation of the T cell receptor (TCR) with antigen/MHC proteins must be accompanied by conjugation of T cell counterreceptors (CD28 or CTLA-4) with costimulatory molecules CD80 or CD86 (B7-1 or B7-2) on antigen presenting cells (APC) to avert T cell anergy, and to provide essential signals for T cell activation and cytokine production. However, T cells and APC express changing patterns of counterreceptors and costimulatory molecules during the immune response. To determine the involvement of CD80 and CD86 in costimulation of T cell cytokine production, T cells were incubated with peritoneal exudate macrophages, which express CD80 and CD86, and stimulated in vitro for 48 or 72 hrs with anti-CD3 in the presence or absence of blocking antibody to CD80 or CD86. Alternatively, enriched anti-CD3 stimulated T cells were costimulated with antibody to CD28 and CTLA-4. Production of T cell IL-2, IL-4, and IL-5 was depressed in the presence of anti-CD86 but not anti-CD80. Production of IFN-γ was significantly blocked by either anti-CD80 and anti-CD86. Anti-CD28 was a potent costimulator of IFN-γ and IL-2 production, but a less potent costimulator of IL-4 and IL-5 production. The data suggest that T cell counterreceptors and APC costimulatory molecules act with varying efficacies at stimulating production of T cell cytokines. 相似文献
14.
Thomas M. Petro Swey-Shen A. Chen Robert B. Panther 《Immunological investigations》1995,24(6):965-976
Conjugation of the T cell receptor (TCR) with antigen/MHC proteins must be accompanied by conjugation of T cell counterreceptors (CD28 or CTLA-4) with costimulatory molecules CD80 or CD86 (B7-1 or B7-2) on antigen presenting cells (APC) to avert T cell anergy, and to provide essential signals for T cell activation and cytokine production. However, T cells and APC express changing patterns of counterreceptors and costimulatory molecules during the immune response. To determine the involvement of CD80 and CD86 in costimulation of T cell cytokine production, T cells were incubated with peritoneal exudate macrophages, which express CD80 and CD86, and stimulated in vitro for 48 or 72 hrs with anti-CD3 in the presence or absence of blocking antibody to CD80 or CD86. Alternatively, enriched anti-CD3 stimulated T cells were costimulated with antibody to CD28 and CTLA-4. Production of T cell IL-2, IL-4, and IL-5 was depressed in the presence of anti-CD86 but not anti-CD80. Production of IFN-γ was significantly blocked by either anti-CD80 and anti-CD86. Anti-CD28 was a potent costimulator of IFN-γ and IL-2 production, but a less potent costimulator of IL-4 and IL-5 production. The data suggest that T cell counterreceptors and APC costimulatory molecules act with varying efficacies at stimulating production of T cell cytokines. 相似文献
15.
Massive production of Th2 cytokines by human CD4+ effector T cells transiently expressing the natural killer cell marker CD57/HNK1.
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We have reported previously that uncommitted human CD4+ CD45RO- T cells default to the T-helper type 1 (Th1) pathway, if they are costimulated by anti-CD3 plus anti-CD28 monoclonal antibodies (mAb). In contrast, 5% of the uncommitted T cells differentiate into Th2 cells, if they are stimulated by anti-CD28 plus interleukin-2 (IL-2) in the absence of T-cell receptor (TCR) signals. The anti-CD28/IL-2-induced proliferation (and the resulting Th2 commitment) was not affected by neutralizing anti-IL-4 mAb, suggesting a non-conventional IL-4-independent Th2 differentiation pathway. Here we report that the respective CD4+ Th2 cells (but not the Th1 cells) coexpressed the natural killer (NK) cell marker HNK1/CD57. Expression of CD57 on Th2 cells required CD28 stimulation, and was suppressed by CD3/TCR signals. However, Th2 effector cells displayed a TCR V beta-chain usage comparable to that of committed Th1 cells (with V beta 8 dominating). Our data suggest that expression of CD57 on human CD4 T cells may be associated with defined stages of Th2 cell activation/differentiation, and may not necessarily characterize a separate T-cell lineage. The induction of cytokine production and B-cell helper function in both Th1 and Th2 populations required CD3/TCR signalling in costimulation with anti-CD28 or IL-2. Importantly, anti-CD28/IL-2-primed Th2 cells readily secreted IL-4 and induced IgE production by surface IgE- B cells in response to the first TCR signal and independent of previous contact with IL-4. Therefore, CD4+ CD57+ T cells responded comparably to murine CD4+ NK1.1+ T cells, which are critical for the development of Th2/IgE immune responses in vivo. The possible role of human CD4+ CD57/HNK1+ Th2-like cells in cancer, infection and allergy is discussed. 相似文献
16.
TCR-mediated target cell lysis by CD4+NK1+ liver T lymphocytes 总被引:2,自引:0,他引:2
In the liver, an unusual T lymphocyte population exists with the intriguing
phenotype CD4+NK1+ TCR alpha beta int. Thus far, functions of these
lymphocytes remained elusive. Recently, however, CD4+NK1+ liver T
lymphocytes have been shown to produce cytokines. Here we show that sorted
CD4+NK1+ liver lymphocytes from naive mice lyse target cells after TCR
alpha beta or CD3, but not TCR gamma delta, engagement. Liver lymphocytes
from beta 2-microglobulin-deficient gene disruption mutant mice failed to
express such cytolytic activities and in vivo treatment with anti-NK1.1 mAb
or anti-CD4 mAb, but not anti-CD8 mAb, markedly reduced target cell lysis.
In vivo administration or rIL-12 impaired TCR alpha beta-mediated target
cell lysis by liver lymphocytes. A similar down-regulation of cytolytic
activities was observed with liver lymphocytes from mice infected with
Listeria monocytogenes or Mycobacterium bovis BCG, which are potent IL-12
inducers. We anticipate (i) that cytolytic CD4+NK1+ T lymphocytes
contribute to immunosurveillance of inflammatory processes in the liver and
(ii) that they are influenced by IL-12.
相似文献
17.
Diaz LA Jr; Friedman AW; He X; Kuick RD; Hanash SM; Fox DA 《International immunology》1997,9(9):1221-1231
CD98 is a 125 kDa heterodimer, which is strongly expressed on the surface
of activated and proliferating cells. Its expression is strikingly
regulated during T cell differentiation and activation, but the role of
CD98 during T lymphocyte responses is not yet understood. We report here
that proliferation of resting peripheral blood mononuclear cells (PBMC)
induced by lectin, superantigen (SAg) or conventional antigens was blocked
by anti-CD98 heavy chain (CD98hc) mAb. In contrast, anti-CD98hc did not
block responses of T cell clones or lines. Anti-CD98hc inhibited IL-2
receptor expression and progression of T cells from G1 to S phase, but did
not reduce expression of the IL-2 gene. Anti-CD98hc mAb did not regulate
the initial activation events involving the TCR and co-receptor structures,
but instead inhibited T lymphocyte responses even when added 18 h or more
after the activation stimulus. Further experiments demonstrated that
anti-CD98 was not directly affecting T cells in this system, but was
instead acting on accessory cells. This was supported using a novel
xenogeneic system that takes advantage of the lack of xenoreactivity of
purified human T cells against mouse splenocytes. Despite absence of a
direct xenoresponse to murine spleen cells, human T cells were activated by
SAg presented by murine splenic antigen-presenting cells (APC). Murine
anti-human CD98hc did not block T cell proliferation in this system.
Furthermore, responses using monocyte-depleted PBMC as APC were not blocked
by anti-CD98hc. Taken together, the present data suggests that triggering
of human monocyte CD98 can suppress T cell proliferation by a process that
halts progression through the cell cycle of recently activated T
lymphocytes. This may represent a novel pathway for monocyte regulation of
T cell activation.
相似文献
18.
Tamauchi H Yoshida Y Sato T Hachimura S Inoue M Kaminogawa S Habu S 《Immunobiology》2005,210(9):709-721
Intraepithelial lymphocytes (IELs) are considered to drive immune surveillance of the epithelial layer to the mucosa, which is initially exposed to exogenous antigens. However, how IELs are activated by orally administered antigens remains unclear. To clarify this mechanism, we fed ovalbumin (OVA) to T cell receptor transgenic (TCR-Tg) mice with OVA-specific MHC class II-restricted TCR and found that the cytotoxic activity of IELs was increased against both NK and LAK target cells, but notably reduced after depleting CD8 + IELs. Cytoplasmic staining showed that the production of IFN-gamma and IL-2 was increased in mice fed with OVA both in the supernatant of cultured IELs with immobilized anti-CD3 mAb and in fresh CD4+ IELs. In contrast, the cytotoxic activity against NK and LAK target cells and the production of IL-2 and IFN-gamma was decreased in splenic T cells from mice fed with OVA. However, when the splenic T cells from these mice were cultured with OVA and IL-2, IFN-gamma production recovered. The decreased response demonstrated the clonal anergy of T cells. Furthermore, tumor growth was enhanced in TCR-Tg mice carrying an OVA-transfected counterpart A20 B cell lymphoma (OVA-A20) and fed with OVA. These results indicate that the oral administration of soluble antigens can activate CD4+ IELs in an antigen-specific manner but induces hyporesponsiveness in the spleen. In addition, Th1-type cytokines produced by activated CD4+ IEL might provide a bystander effect on the cytotoxic activity of IELs. 相似文献
19.
Watanabe T Masuyama J Sohma Y Inazawa H Horie K Kojima K Uemura Y Aoki Y Kaga S Minota S Tanaka T Yamaguchi Y Kobayashi T Serizawa I 《Clinical immunology (Orlando, Fla.)》2006,120(3):247-259
We previously reported that 4C8 monoclonal antibody (mAb) provides a costimulatory signal to human CD4+ T cells and consequently induces regulatory T (Treg) cells, which are hypo-responsive and suppress the polyclonal response of bystander CD4+ cells in a contact-dependent manner. In this study, we identified the antigen of 4C8 mAb as CD52. Costimulation with Campath-1H, a humanized anti-CD52 mAb, also induced Treg cells. Anti-CD52-induced Treg cells suppressed the proliferation of both CD4+ and CD8+ T cells provided with polyclonal or allogeneic stimulation. When Treg cells were induced from Staphylococcal enterotoxin B (SEB) treated cells, they suppressed the response to SEB more efficiently than that to another superantigen, SEA. Furthermore, anti-CD52-induced Treg cells could be expanded by culture with IL-2 followed by CD52-costimulation, and co-injection of expanded Treg cells suppressed lethal xenogeneic graft versus host disease (GvHD) reactions in SCID mice caused by human peripheral blood mononuclear cells (PBMCs). 相似文献
20.
Superantigen-induced peripheral T-cell deletion: the effects of chemical modification of antigen-presenting cells, interleukin-4 and glucocorticoid hormones. 总被引:2,自引:0,他引:2
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Experiments were performed to evaluate the role of antigen-presenting cells (APC) and the effect of interleukin-4 (IL-4) and glucocorticoid hormone (GCH) exposure on the in vitro deletion of CD4+ CD8- and CD8+ CD4- T cells by staphylococcal enterotoxin B (SEB). APC fixation with the chemical cross-linker 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (ECDI) inhibited their capacity to induce SEB-specific deletion of mature T lymphocytes. Deletion was not influenced by treatment with anti-CD28 antibodies, which modulate T-cell activation. However, it was augmented by IL-4, known to counteract anti-CD3- and GCH-induced thymocyte apoptosis, and was inhibited by dexamethasone (DEX). These results indicate that metabolically active APC are required for deletion of antigen-specific mature T cells and suggest that IL-4 and GCH can modulate this phenomenon in vitro. 相似文献