牛磺酸对增减大鼠心肌细胞感染柯萨奇B3病毒的影响

目的 探讨牛磺酸对由柯萨奇Ｂ３病毒感染的大鼠心肌细胞的抗病毒和保护作用,为该药的临床应用提供理论依据。方法 取新生ＳＤ大鼠的乳鼠心室肌肌制备博动心肌细胞,培养４８ｈ后接种１０＾３ＴＣＩＤ５０的柯萨奇Ｂ３病毒做为实验性病毒性心肌炎模型。结果牛磺酸明显减轻感染柯萨奇Ｂ３病毒心肌细胞的病变,使细胞博动增强;提高心肌细胞的琥珀酸脱氢酶活性,降低乳酸脱氢酶活性;抑制心肌细胞感染柯萨奇Ｂ３病毒后导致的乳酸脱     

草苁蓉提取物对感染柯萨奇B3病毒的大鼠心肌细胞抗氧化作用的影响

[目的]探讨草苁蓉提取物对柯萨奇B3病毒感染的大鼠心肌细胞的抗氧化作用。[方法]取新生SD大鼠的乳鼠心室肌制备搏动心肌细胞,培养48h后接种10^3TCID50柯萨奇B3病毒做为实验性病毒性心肌炎模型。[结果]草苁蓉提取物明显减轻感染柯萨奇B3病毒的心肌细胞病变,使细胞搏动增强,明显降低心肌细胞内的丙二醛含量,显升高超氧化物歧化酶活性。[结论]草苁蓉提取物对感染柯萨奇B3病毒的SD大鼠培养心肌细胞具有显的抗氧化作用。     

病毒性心肌炎感染模型——大鼠搏动心肌细胞培养的柯萨奇B_2病毒感染研究

新生培养的 Sprague-Dawley 大鼠搏动心肌细胞感染柯萨奇 B_2病毒后,逐步出现细胞病变、细胞停止搏动,培养液中乳酸脱氢酶(LDH)及谷草转氨酶(SGOT)增高,核素标记检测有细胞毒性增加等改变.此模型可用于研究某些药物对病毒性心肌炎的防治观察。     

牛磺酸对培养大鼠心肌细胞感染柯萨奇B_3病毒的影响

[目的 ]探讨牛磺酸对由柯萨奇B3 病毒感染的大鼠心肌细胞的抗病毒和保护作用 ,为该药的临床应用提供理论依据 .[方法 ]取新生SD大鼠的乳鼠心室肌制备搏动心肌细胞 ,培养 48h后接种10 3TCID5 0的柯萨奇B3 病毒做为实验性病毒性心肌炎模型 .[结果 ]牛磺酸明显减轻感染柯萨奇B3 病毒心肌细胞的病变 ,使细胞搏动增强 ;提高心肌细胞的琥珀酸脱氢酶活性 ,降低乳酸脱氢酶活性 ;抑制心肌细胞感染柯萨奇B3 病毒后导致的乳酸脱氢酶和谷草转氨酶释放 .牛磺酸提高正常心肌细胞和感染柯萨奇B3 病毒的心肌细胞存活率 ,其作用具有剂量依赖性 .[结论 ]牛磺酸对感染柯萨奇B3 病毒的大鼠搏动心肌细胞具有一定的抗病毒和保护心肌细胞的作用     

生脉散合炙甘草汤加减液对感染柯萨奇B_3病毒的培养大鼠心肌细胞的影响

[目的 ]探讨生脉散合炙甘草汤加减液对病毒性心肌炎的影响 ,为该药的临床应用提供实验依据 .[方法 ]取新生SD大鼠心室肌培养搏动心肌细胞 48h后 ,添加 10 3 TCID50 的柯萨奇B3 病毒作病毒性心肌炎模型 .实验分 4个组进行 ,即正常组、病毒组、干扰素组、生脉散合炙甘草汤加减液组 .[结果 ]生脉散合炙甘草汤加减液明显减轻心肌细胞感染病毒后病变 ,改善细胞搏动 ,明显降低心肌细胞释放酶(乳酸脱氢酶、磷酸肌酸激酶 )活性 ,减轻超微结构的病变 .[结论 ]生脉散合炙甘草汤加减液对感染柯萨奇B3 病毒的SD大鼠培养心肌细胞具有明显的抗病毒和保护作用     

苦瓜蛋白对柯萨奇3型病毒感染大鼠心肌细胞的保护作用

目的 在病毒性心肌炎心肌细胞模型上观察苦瓜蛋白对柯萨奇3型病毒(CVB3)感染SD大鼠心肌细胞的保护作用及其机制. 方法 体外原代培养SD大鼠心肌细胞,以CVB3感染培养大鼠心肌细胞,复制病毒性心肌炎心肌细胞模型,分别测定心肌细胞搏动百分比(PBC)、半数有效率(ED)、细胞病变(CPE)、心肌酶谱、半感染致死量(TCID50),分析其对心肌细胞的保护作用. 结果 苦瓜蛋白的ED值为0.045 mg/mL,80、40、20、10 μg/mL苦瓜蛋白可显著提高心肌细胞PBC、降低病毒的复制,80、40 μg/mL苦瓜蛋白可减少CPE,降低心肌酶水平. 结论 苦瓜蛋白对感染CVB3的心肌细胞有较强的保护作用.     

贯众水提取液对感染柯萨奇B_3病毒的培养大鼠心肌细胞的影响

[目的 ]探讨贯众水提取液对感染柯萨奇B3 病毒的大鼠心肌细胞的抗病毒和保护作用 .[方法 ]实验取新生SD大鼠的乳鼠心室肌制备搏动心肌细胞 ,培养 48h后接种 10 3 TCID50 的柯萨奇B3 病毒做为实验性病毒性心肌炎模型 .[结果 ]贯众水提取液和炙甘草汤的同时加药组和 1h后加药组大鼠心肌细胞释放的乳酸脱氢酶和天冬氨酸转氨酶活性明显降低 ,感染 12h后加药组效果明显减弱 ,而 2 4h后加药组则无效 .[结论 ]贯众水提取液对感染柯萨奇B3 病毒的SD大鼠培养心肌细胞具有抗病毒和保护作用 ,应早期应用     

生脉散合炙甘草汤加减液对染病毒性心肌炎保护作用的实验研究

目的:探讨生脉散合炙甘草汤加减液对病毒性心肌炎的保护作用。方法:实验取新生SD大鼠心室肌制备搏动心肌细胞,培养48 h后添加103TCID50的柯萨奇B3病毒作为实验性病毒性心肌炎模型。实验分3个组进行,即正常对照组、病毒对照组、生脉散合炙甘草汤加减液组(治疗组)。结果:生脉散合炙甘草汤加减液可明显提高细胞搏动百分比,明显增加细胞内糖原颗粒数目,增强琥珀酸脱氢酶活性,降低乳酸脱氢酶活性。结论:生脉散合炙甘草汤加减液在体外对感染病毒大鼠心肌细胞具有明显的保护作用。     

实验性病毒性心肌炎药物治疗及凋亡的机制

目的以病毒性心肌炎（ＶＭＣ）细胞感染模型为基础，研究柯萨奇Ｂ３病毒（ＣＶＢ３）细胞感染与心肌细胞凋亡的关系，并观察板蓝根对实验性ＶＭＣ的治疗效果。方法第一步建立稳定的ＶＭＣ细胞感染模型；第二步在ＣＶＢ３病毒感染心肌细胞后２４ｈ电镜观察超微结构改变，流式细胞仪检测“凋亡峰”，ＥＬＩＳＡ法定量检测ＣＶＢ３病毒感染后１，２，４，７，２４ｈ心肌细胞凋亡的情况；第三步将不同浓度的板蓝根与ＣＶＢ３病毒作用后在心肌细胞上检测病毒的ＴＣＩＤ５０。根据药物毒性测定的结果，取最大无毒浓度以下两种不同浓度的板蓝根按两种方式加药：（１）ＣＶＢ３病毒感染细胞后加药；（２）病毒先与药物作用后再感染细胞进行实验。以黄芪作为治疗阳性对照组，另设病毒感染组、正常细胞对照组。于药物作用４８ｈ后观察各组细胞搏动百分比、细胞病变（ＣＰＥ）、超微结构变化，并测定培养液中心肌酶水平，进行体外、细胞内药物疗效观察。结果（１）对ＣＶＢ３病毒感染后细胞的形态学检测（ＣＰＥ３＋～４＋），电镜下细胞内见包涵体，病毒颗粒及细胞培养液心肌酶（ＡＳＴ、ＬＤＨ、ＣＫ、ＣＫＭＢ、ＨＢＤＨ）水平升高，证实所建立的ＶＭＣ细胞感染模型是稳定的。（２）电镜观察ＣＶＢ３病毒感染心     

小柴胡汤分解剂抗柯萨奇B3病毒感染及其对心肌炎防治作 …

目的：探讨小柴胡汤分解剂在体外及体内抗柯萨奇Ｂ３病毒（ＣＶＢ３）及保护心肌细胞的作用。方法：用小柴胡汤分解剂通过体外微量细胞培养,作用于被ＣＶＢ３直接损伤的细胞模型及经腹腔感染ＣＶＢ３ｍＢａｌｂ／ｃ小鼠心肌炎模型,分析了不同浓度分解剂的效应。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.