奥沙利铂联合亚叶酸钙和5-Fu治疗大肠癌的药物治疗效果及不良反应观察

目的:评价奥沙利铂联合亚叶酸钙和大剂量5-Fu持续48h静脉滴注治疗高龄晚期大肠癌的疗效和安全性。方法:全组病例采用静脉滴注奥沙利铂100mg/m2亚叶酸钙200mg/m2,亚叶酸钙滴注之后用5-Fu 0.5g静脉推注,接着用5-Fu3.0g/m2,持续静脉滴注48h,每2周1次,2次为1周期。结果:43例病例中,平均疗程数为4个周期,其中CR 2例,PR 22例,SD15例,PD 4例,总有效率(CR+PR)为55.8%。不良反应为恶心、呕吐和骨髓抑制,但多为I~Ⅱ度,一过性感觉异常。结论:奥沙利铂联合亚叶酸钙和5-Fu治疗高龄晚期大肠癌疗效较高,不良反应轻而且安全,患者容易耐受,值得推广使用。     

奥沙利铂联合5-氟脲嘧啶持续静脉滴注48h及亚叶酸钙(双周疗法)治疗晚期大肠癌34例的临床观察

目的:观察奥沙利铂联合5-氟脲嘧啶持续静脉滴注48h及亚叶酸钙(双周疗法)治疗晚期大肠癌的近期方疗效和不良反应.方法:全组病例采用静脉滴注奥沙利铂100mg/m2、亚叶酸钙200mg/m2,滴完亚叶酸钙之后5-FU500mg静脉注射,接着5-FU 3.0 g/m2用输液泵持续静脉滴注 48h,每2周1次,2次为1周期.结果:全组CR 1例,PR15例,SD 9例,PD9例,总有效率(CR PR)为47.06%(16/34),主要不良反应为恶心、呕吐、骨髓抑制、口腔粘膜炎、外周静脉炎,多数为Ⅰ～Ⅱ度以及轻度外周神经毒性.结论:奥沙利铂联合5-氟脲嘧啶持续静脉滴注48h及亚叶酸钙(双周疗法)治疗晚期大肠癌疗效较好,不良反应轻,患者耐受性良好,并且经济实用.     

奥沙利铂联合5-氟脲嘧啶、亚叶酸钙治疗晚期大肠癌的临床观察

目的:观察奥沙利铂联合5-氟脲嘧啶、亚叶酸钙治疗晚期大肠癌的临床疗效及不良反应。方法:评价分析26例经组织病理学确诊的晚期大肠癌的患者,应用奥沙利铂130mg/m2,第1天静脉滴注;亚叶酸钙150mg/m2,第1~5天静脉滴注;5-氟脲嘧啶500mg/m2,第1~5天静脉滴注;21d为1个周期,观察治疗2~3个周期后的临床疗效及不良反应。结果:26例患者中,CR 3例,PR 8例,SD 11例,PD 4例,总有效率(CR+PR)42.31%。主要的不良反应有骨髓抑制、消化道反应、感觉神经毒性,多为Ⅰ°~Ⅱ°。结论:奥沙利铂联合5-氟脲嘧啶、亚叶酸钙治疗晚期大肠癌有效率较高,不良反应轻。     

FOL FOX方案治疗晚期大肠癌临床观察

目的观察奥沙利铂（L—OHP）联合氟脲嘧啶（5-Fu）及亚叶酸钙（CF）治疗晚期大肠癌的疗效和不良反应。办法对48例患者应用奥沙利铂＋亚叶酸钙＋氟脲嘧啶联合治疗5周期，评价疗效。结果48例患者中CR2例（4．17％），PR18例（37．5％），SD16例（33．3％），PD12例（25％）。主要的不良反应为消化道症状、骨髓抑制和感觉神经毒性。结论奥沙利铂联合亚叶酸钙和氟脲嘧啶不良反应少，外周神经毒性可以耐受且可逆，是一种安全、有效的治疗晚期大肠癌的药物。     

奥沙利铂联合氟尿嘧啶/亚叶酸钙治疗晚期大肠癌的疗效观察

目的：观察奥沙利铂联合氟脲嘧啶/亚叶酸钙治疗晚期结肠癌的疗效与安全性。方法：32例晚期大肠癌患者给予奥沙利铂130mg/m2静脉滴注,持续3h,第1天;亚叶酸钙200mg/m2静脉滴注,持续2h第1～2天;氟脲嘧啶500mg静脉推注,随后1000mg/m2持续泵22h,第1～2天;21天重复1次,化疗2周期评价疗效。按WHO标准评价近期疗效和不良反应。结果：入组32例晚期大肠癌患者,经治疗后CR1例（3.1%）,PR12例（37.5%）,SD11例（34.4%）,PD8例（25%）,总有效率40.6%。主要不良反应为骨髓抑制、黏膜炎、消化道反应。全组患者无化疗相关死亡。结论：该方案疗效高,不良反应轻,患者均能耐受。     

草酸铂联合氟脲嘧啶、亚叶酸钙治疗晚期胃癌30例效果观察

目的:观察草酸铂联合氟脲嘧啶和亚叶酸钙治疗晚期胃癌的近期疗效和不良反应。方法:化疗OLF3方案:草酸铂30mg/m2,静滴第1日;亚叶酸钙200mg/m2,静滴2小时,第1~3日;5-氟脲嘧啶400mg/m2,快速静注后再用5-氟脲嘧啶600mg/m2持续静滴22小时,第1~3日;21日为1周期,完成2周期后评定疗效,有效病例4周后确认。结果:全组完全缓解3例,部分缓解11例,总有效率(CR+PR)46.6%;主要不良反应为轻度血液学毒性和外周感觉神经毒性。结论:含草酸铂联合5-Fu和亚叶酸钙治疗晚期胃癌有效且毒性较小。     

OLF方案治疗晚期胃肠道恶性肿瘤37例疗效观察

目的观察奥沙利铂（OXA）联合5-氟脲嘧啶（5-Fu）和亚叶酸钙（CF）治疗晚期胃肠道恶性肿瘤的近期疗效及不良反应。方法采用OXA 130mg/m^2,静脉滴注2h,第1天;CF 100mg/m^2,静脉滴注2h,然后5-Fu 750mg/m^2,静脉滴注4h,第1-5天。3周为1个周期,2个周期化疗结束后评价疗效,此法共治疗37例患者。结果CR 3例,PR 15例,SD 15例,PD 4例。有效率胃癌44.4%,结肠癌53.3%,直肠癌46.2%。不良反应多为Ⅰ~Ⅱ度,Ⅲ~Ⅳ度发生率较低。结论OXA联合5-Fu和亚叶酸钙方案治疗晚期胃肠道恶性肿瘤有效率高,不良反应轻,耐受性好,值得推广。     

奥沙利铂联合亚叶酸钙、氟尿嘧啶治疗晚期胃癌

目的: 评价奥沙利铂联合氟尿嘧啶(5-Fu)、亚叶酸钙(CF)治疗晚期胃癌的疗效及安全性。方法: 奥沙利铂(OXA)130mg/m2,静脉滴注(静滴)2 h,第1天;亚叶酸钙200 mg静滴1 h,第1~5天;5-Fu 350 mg/m2静滴4 h,第1~5天;每3周重复一周期,连用2周期后评价疗效。结果: 观察28例,其中CR 1例,PR 11例,SD 10例,PD 6例,总有效率42.9%。主要不良反应为白细胞下降、恶心呕吐及周围神经毒性。结论: 奥沙利铂联合化疗治疗晚期胃癌疗效较好,且不良反应小。     

国产奥沙利铂联合亚叶酸钙和5-氟尿嘧啶治疗晚期结直肠癌临床观察

目的　评价奥沙利铂联合亚叶酸钙、5—氟尿嘧啶 (OXA -Lv - 5 -Fu)方案治疗晚期结直肠癌的疗效与安全性。方法　第 1天奥沙利铂 (OXA) 130mg/m2 ,静脉滴注 4h ;亚叶酸钙 (Lv) 2 0 0mg静脉滴注 ,5—氟尿嘧啶 ( 5 -Fu) 0 2 5于Lv滴完后静脉推注 ,5 -Fu 0 5持续静脉滴注 6～ 8h ,连用 5d ,每 3周重复。结果　全组患者有效率 (CR +PR)为 4 2 31%。不良反应为恶心呕吐 ,骨髓抑制 ,但多为Ⅰ -Ⅱ°一过性感觉异常。结论　奥沙利铂联合亚叶酸钙和 5 -氟尿嘧啶治疗晚期结直肠癌疗效较高 ,不良反应轻而且安全。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.