Fas和bcl-2在骨肉瘤中的表达对其发生和预后的意义

目的：研究Fas和bcl-2在骨肉瘤中的表达，探讨其在骨肉瘤发生及其预后中的可能作用。方法：用SP免疫组化方法检测Fas和bcl-2在38例骨肉瘤及20例骨软骨瘤中的表达。结果：Fas在骨肉瘤中表达阳性率为73．7％，明显低于对照组骨软骨瘤中95．0％的阳性表达率(r=3．874，P&;lt;0．05)；bcl-2在骨肉瘤中表达阳性率为63．2％，明显高于对照组骨软骨瘤中20．O％的阳性表达率(χ^2=9．774，P&;lt;0．01)。骨肉瘤中Fas和bcl-2的表达呈显著负相关(r=-0．333．P&;lt;0．05)结论：Fas表达减低和bcl-2表达升高与骨肉瘤的发生机制有关，且具有相关性。     

Bcl-2和Caspase-3在骨肉瘤中的表达及其临床意义

目的研究Bcl-2和Caspase-3蛋白在骨肉瘤中的表达情况,探讨Bcl-2和Caspase-3基因在骨肉瘤发生和发展中的作用,分别研究两者与骨肉瘤Ennecking外科分期之间的关系。方法采用免疫组化SP方法观察30例骨肉瘤中的Bcl-2和Caspase-3蛋白表达情况,并对结果用SPSS11．0统计软件进行分析。结果在骨肉瘤中,Bcl-2和Caspase-3蛋白的阳性率分别为73．3％、86．7％,两者在骨肉瘤和骨软骨瘤中的阳性表达率比较,差异均有统计学意义（P〈0．01）。骨肉瘤Ⅰ～Ⅲ期中,Bcl-2和Caspase-3蛋白的阳性率比较,差异无统计学意义,Bcl-2和Caspase-3的表达不存在相关关系。结论骨肉瘤发生与细胞凋亡失控有关,但骨肉瘤的发生和发展是多基因、多步骤的复杂过程,Bcl-2和Caspase-3表达失控只是其中的一部分原因;利用SP免疫组化方法检测骨肉瘤中Bcl-2和Caspase-3蛋白的表达具有较强的敏感性,但同时,Bcl-2和Caspase-3蛋白的表达程度与外科Ennecking分期没有明显的相关性,所以其表达不能作为判断骨肉瘤恶性程度以及预后的标准。     

Bcl-2和Caspase-3在骨肉瘤中的表达及其临床意义

Objective To study the expression levels of Bcl-2 and Caspase-3 protein in osteosarcoma,and discuss the role of Bcl-2 and Caspase-3 gene in the occurrence and development of human osteosarcoma,as well the relationship between their expression levels and Ennecking staging.Methods SP immunohistochemical staining assay was applied to detecting the expression levels of Bcl-2 and Caspase-3 protein in 30 aptients with osteosarcoma,and the results were analyzed with SPSS11.0 software.Results The positive rate of Bcl-2 and Caspase-3 protein in osteosarcoma was respectively 73.3% and 86.7%.There were prominent differences of positive rate of Bcl-2 and Caspase-3 between osteosarcoma and osteochondroma groups.The expression rates of Bcl-2 and Caspase-3 protein had no significant difference among osteosarcoma Ennecking grade Ⅰ to grade Ⅲ respectively.In osteosarcoma group,no correlation existed between the expression rates of Bcl-2 and Caspase-3(P＞0.05).Conclusion Although the occurrence and development of human osteosarcoma are correlated with the dropout of apoptosis,they are implicated in a complicated course participated by multiple genes and procedures.Bcl-2 and Caspase-3 gene play a certain role partly.SP immunohistochemical staining assay shows a higher sensitivity in detecting the expression of Bcl-2 and Caspase-3 protein in osteosarcoma;however,their expression levels were not correlative to Ennecking staging.Therefore,the expression of Bcl-2 and Caspase-3 may not be used as the criteria of the malignant degree and prognosis of osteosarcoma.     

Bcl-2和Caspase-3在骨肉瘤中的表达及其临床意义

Objective To study the expression levels of Bcl-2 and Caspase-3 protein in osteosarcoma,and discuss the role of Bcl-2 and Caspase-3 gene in the occurrence and development of human osteosarcoma,as well the relationship between their expression levels and Ennecking staging.Methods SP immunohistochemical staining assay was applied to detecting the expression levels of Bcl-2 and Caspase-3 protein in 30 aptients with osteosarcoma,and the results were analyzed with SPSS11.0 software.Results The positive rate of Bcl-2 and Caspase-3 protein in osteosarcoma was respectively 73.3% and 86.7%.There were prominent differences of positive rate of Bcl-2 and Caspase-3 between osteosarcoma and osteochondroma groups.The expression rates of Bcl-2 and Caspase-3 protein had no significant difference among osteosarcoma Ennecking grade Ⅰ to grade Ⅲ respectively.In osteosarcoma group,no correlation existed between the expression rates of Bcl-2 and Caspase-3(P＞0.05).Conclusion Although the occurrence and development of human osteosarcoma are correlated with the dropout of apoptosis,they are implicated in a complicated course participated by multiple genes and procedures.Bcl-2 and Caspase-3 gene play a certain role partly.SP immunohistochemical staining assay shows a higher sensitivity in detecting the expression of Bcl-2 and Caspase-3 protein in osteosarcoma;however,their expression levels were not correlative to Ennecking staging.Therefore,the expression of Bcl-2 and Caspase-3 may not be used as the criteria of the malignant degree and prognosis of osteosarcoma.     

Bcl-2和Caspase-3在骨肉瘤中的表达及其临床意义

Objective To study the expression levels of Bcl-2 and Caspase-3 protein in osteosarcoma,and discuss the role of Bcl-2 and Caspase-3 gene in the occurrence and development of human osteosarcoma,as well the relationship between their expression levels and Ennecking staging.Methods SP immunohistochemical staining assay was applied to detecting the expression levels of Bcl-2 and Caspase-3 protein in 30 aptients with osteosarcoma,and the results were analyzed with SPSS11.0 software.Results The positive rate of Bcl-2 and Caspase-3 protein in osteosarcoma was respectively 73.3% and 86.7%.There were prominent differences of positive rate of Bcl-2 and Caspase-3 between osteosarcoma and osteochondroma groups.The expression rates of Bcl-2 and Caspase-3 protein had no significant difference among osteosarcoma Ennecking grade Ⅰ to grade Ⅲ respectively.In osteosarcoma group,no correlation existed between the expression rates of Bcl-2 and Caspase-3(P＞0.05).Conclusion Although the occurrence and development of human osteosarcoma are correlated with the dropout of apoptosis,they are implicated in a complicated course participated by multiple genes and procedures.Bcl-2 and Caspase-3 gene play a certain role partly.SP immunohistochemical staining assay shows a higher sensitivity in detecting the expression of Bcl-2 and Caspase-3 protein in osteosarcoma;however,their expression levels were not correlative to Ennecking staging.Therefore,the expression of Bcl-2 and Caspase-3 may not be used as the criteria of the malignant degree and prognosis of osteosarcoma.     

Bcl-2和Caspase-3在骨肉瘤中的表达及其临床意义

Objective To study the expression levels of Bcl-2 and Caspase-3 protein in osteosarcoma,and discuss the role of Bcl-2 and Caspase-3 gene in the occurrence and development of human osteosarcoma,as well the relationship between their expression levels and Ennecking staging.Methods SP immunohistochemical staining assay was applied to detecting the expression levels of Bcl-2 and Caspase-3 protein in 30 aptients with osteosarcoma,and the results were analyzed with SPSS11.0 software.Results The positive rate of Bcl-2 and Caspase-3 protein in osteosarcoma was respectively 73.3% and 86.7%.There were prominent differences of positive rate of Bcl-2 and Caspase-3 between osteosarcoma and osteochondroma groups.The expression rates of Bcl-2 and Caspase-3 protein had no significant difference among osteosarcoma Ennecking grade Ⅰ to grade Ⅲ respectively.In osteosarcoma group,no correlation existed between the expression rates of Bcl-2 and Caspase-3(P＞0.05).Conclusion Although the occurrence and development of human osteosarcoma are correlated with the dropout of apoptosis,they are implicated in a complicated course participated by multiple genes and procedures.Bcl-2 and Caspase-3 gene play a certain role partly.SP immunohistochemical staining assay shows a higher sensitivity in detecting the expression of Bcl-2 and Caspase-3 protein in osteosarcoma;however,their expression levels were not correlative to Ennecking staging.Therefore,the expression of Bcl-2 and Caspase-3 may not be used as the criteria of the malignant degree and prognosis of osteosarcoma.     

Bcl-2和Caspase-3在骨肉瘤中的表达及其临床意义

Objective To study the expression levels of Bcl-2 and Caspase-3 protein in osteosarcoma,and discuss the role of Bcl-2 and Caspase-3 gene in the occurrence and development of human osteosarcoma,as well the relationship between their expression levels and Ennecking staging.Methods SP immunohistochemical staining assay was applied to detecting the expression levels of Bcl-2 and Caspase-3 protein in 30 aptients with osteosarcoma,and the results were analyzed with SPSS11.0 software.Results The positive rate of Bcl-2 and Caspase-3 protein in osteosarcoma was respectively 73.3% and 86.7%.There were prominent differences of positive rate of Bcl-2 and Caspase-3 between osteosarcoma and osteochondroma groups.The expression rates of Bcl-2 and Caspase-3 protein had no significant difference among osteosarcoma Ennecking grade Ⅰ to grade Ⅲ respectively.In osteosarcoma group,no correlation existed between the expression rates of Bcl-2 and Caspase-3(P＞0.05).Conclusion Although the occurrence and development of human osteosarcoma are correlated with the dropout of apoptosis,they are implicated in a complicated course participated by multiple genes and procedures.Bcl-2 and Caspase-3 gene play a certain role partly.SP immunohistochemical staining assay shows a higher sensitivity in detecting the expression of Bcl-2 and Caspase-3 protein in osteosarcoma;however,their expression levels were not correlative to Ennecking staging.Therefore,the expression of Bcl-2 and Caspase-3 may not be used as the criteria of the malignant degree and prognosis of osteosarcoma.     

Bcl-2和Caspase-3在骨肉瘤中的表达及其临床意义

Objective To study the expression levels of Bcl-2 and Caspase-3 protein in osteosarcoma,and discuss the role of Bcl-2 and Caspase-3 gene in the occurrence and development of human osteosarcoma,as well the relationship between their expression levels and Ennecking staging.Methods SP immunohistochemical staining assay was applied to detecting the expression levels of Bcl-2 and Caspase-3 protein in 30 aptients with osteosarcoma,and the results were analyzed with SPSS11.0 software.Results The positive rate of Bcl-2 and Caspase-3 protein in osteosarcoma was respectively 73.3% and 86.7%.There were prominent differences of positive rate of Bcl-2 and Caspase-3 between osteosarcoma and osteochondroma groups.The expression rates of Bcl-2 and Caspase-3 protein had no significant difference among osteosarcoma Ennecking grade Ⅰ to grade Ⅲ respectively.In osteosarcoma group,no correlation existed between the expression rates of Bcl-2 and Caspase-3(P＞0.05).Conclusion Although the occurrence and development of human osteosarcoma are correlated with the dropout of apoptosis,they are implicated in a complicated course participated by multiple genes and procedures.Bcl-2 and Caspase-3 gene play a certain role partly.SP immunohistochemical staining assay shows a higher sensitivity in detecting the expression of Bcl-2 and Caspase-3 protein in osteosarcoma;however,their expression levels were not correlative to Ennecking staging.Therefore,the expression of Bcl-2 and Caspase-3 may not be used as the criteria of the malignant degree and prognosis of osteosarcoma.     

Bcl-2和Caspase-3在骨肉瘤中的表达及其临床意义

Objective To study the expression levels of Bcl-2 and Caspase-3 protein in osteosarcoma,and discuss the role of Bcl-2 and Caspase-3 gene in the occurrence and development of human osteosarcoma,as well the relationship between their expression levels and Ennecking staging.Methods SP immunohistochemical staining assay was applied to detecting the expression levels of Bcl-2 and Caspase-3 protein in 30 aptients with osteosarcoma,and the results were analyzed with SPSS11.0 software.Results The positive rate of Bcl-2 and Caspase-3 protein in osteosarcoma was respectively 73.3% and 86.7%.There were prominent differences of positive rate of Bcl-2 and Caspase-3 between osteosarcoma and osteochondroma groups.The expression rates of Bcl-2 and Caspase-3 protein had no significant difference among osteosarcoma Ennecking grade Ⅰ to grade Ⅲ respectively.In osteosarcoma group,no correlation existed between the expression rates of Bcl-2 and Caspase-3(P＞0.05).Conclusion Although the occurrence and development of human osteosarcoma are correlated with the dropout of apoptosis,they are implicated in a complicated course participated by multiple genes and procedures.Bcl-2 and Caspase-3 gene play a certain role partly.SP immunohistochemical staining assay shows a higher sensitivity in detecting the expression of Bcl-2 and Caspase-3 protein in osteosarcoma;however,their expression levels were not correlative to Ennecking staging.Therefore,the expression of Bcl-2 and Caspase-3 may not be used as the criteria of the malignant degree and prognosis of osteosarcoma.     

Bcl-2和Caspase-3在骨肉瘤中的表达及其临床意义

Objective To study the expression levels of Bcl-2 and Caspase-3 protein in osteosarcoma,and discuss the role of Bcl-2 and Caspase-3 gene in the occurrence and development of human osteosarcoma,as well the relationship between their expression levels and Ennecking staging.Methods SP immunohistochemical staining assay was applied to detecting the expression levels of Bcl-2 and Caspase-3 protein in 30 aptients with osteosarcoma,and the results were analyzed with SPSS11.0 software.Results The positive rate of Bcl-2 and Caspase-3 protein in osteosarcoma was respectively 73.3% and 86.7%.There were prominent differences of positive rate of Bcl-2 and Caspase-3 between osteosarcoma and osteochondroma groups.The expression rates of Bcl-2 and Caspase-3 protein had no significant difference among osteosarcoma Ennecking grade Ⅰ to grade Ⅲ respectively.In osteosarcoma group,no correlation existed between the expression rates of Bcl-2 and Caspase-3(P＞0.05).Conclusion Although the occurrence and development of human osteosarcoma are correlated with the dropout of apoptosis,they are implicated in a complicated course participated by multiple genes and procedures.Bcl-2 and Caspase-3 gene play a certain role partly.SP immunohistochemical staining assay shows a higher sensitivity in detecting the expression of Bcl-2 and Caspase-3 protein in osteosarcoma;however,their expression levels were not correlative to Ennecking staging.Therefore,the expression of Bcl-2 and Caspase-3 may not be used as the criteria of the malignant degree and prognosis of osteosarcoma.     

骨肉瘤组织中Cox-2，Fas的表达及其临床意义

目的研究环氧合酶2（Cox-2），Fas在骨肉瘤中的表达，探讨其在骨肉瘤发生、发展中的作用。方法应用免疫组化免疫组织化学染色法（SP法）检测38例骨肉瘤组织、20例骨软骨瘤组织中Cox-2，Fas的表达。结果22例（57．9％）的骨肉瘤组织表达Cox-2阳性，显著高于骨软骨瘤组织的表达率（25．0％）（P〈0．05）。21．1％的骨肉瘤组织表达Fas阳性，骨软骨瘤阳性率为45．0％（P〈0．01）。骨软骨瘤组织的表达率为20．0％（P〈0．01）。Cox-2表达上调和Fas表达下调与骨肉瘤的临床分期密切相关，Cox-2的表达与Fas的表达密切相关。结论在骨肉瘤组织中Cox-2和Fas的表达异常，提示它们可能对骨肉瘤的发展起重要作用。     

Fas和bcl-2基因与重症肌无力的发生与治疗

背景目前Fas和Bcl-2基因在重症肌无力发病机制中的作用越来越受到重视,国内外已做了较多的研究,阐明调控Fas和Bcl-2基因的表达对重症肌无力发生及其疾病过程中的治疗均具有密切的关系.资料来源应用计算机检索Medline数据库1990-01/2004-12期间的相关文献,检索词为"Fas","Bcl-2","Myasthenia Gravis"及"gene"分别组合进行检索,限定文章语言种类为English.资料选择对资料进行初审,选取包括上述Fas和Bcl-2与重症肌无力的文献,以及有关Fas和Bcl-2基因及基因治疗方面的文献,并且查找全文.资料提炼搜集到大量介绍Fas和Bcl-2基因的文献,21篇有关Fas和Bcl-2与重症肌无力的文献以及32篇重症肌无力有关基因治疗方面的文献,选取有代表性的20篇文章纳入分析.资料综合20篇文献中有8篇关于Fas基因及重症肌无力,5篇关于Bcl-2基因及重症肌无力,7篇关于基因手段治疗重症肌无力及免疫性疾病.结论Fas和Bcl-2基因调节异常、表达失控与T淋巴细胞、胸腺细胞凋,亡缺陷密切相关,与重症肌无力发病密切相关,从基因水平揭示重症肌无力的发病机制在基因水平诱导细胞凋亡以抑制自身抗体产生,将为重症肌无力的治疗提供新的手段.     

Fas和bcf-2基因与重症肌无力的发生与治疗

背景：目前Fas和Bcf-2基因在重症肌无力发病机制中的作用越来越受到重视，国内外已做了较多的研究，阐明凋控如和Bcf-2基因的表达对重症肌无力发生及其疾病过程中的治疗均具有密切的关系。资料来源：应用计算机检索Medline数据库1990—01／2004-12期间的相关文献，检索词为“Fas”，“Bcl-2”，“MyastheniaGravis”及“gene”分别组合进行检索，限定文章语言种类为English。资料选择：对资料进行初审，选取包括上述如和Bcf-2与重症肌无力的文献，以及有关如和Bcf-2基因及基因治疗方面的文献，并且查找全文。资料提炼：搜集到大量介绍如和Bcf-2基因的文献，21篇有关如和Bcf-2与重症肌无力的文献以及32篇重症肌无力有关基因治疗方面的文献，选取有代表性的20篇文章纳入分析。资料综合：20篇文献中有8篇关于如基因及重症肌无力，5篇关于Bcf-2基因及晕症肌无力，7篇关于基冈手段治疗重症肌无力及免疫性疾病。结论：如和Bcf-2基因调节异常、表达失控与T淋巴细胞、胸腺细胞凋亡缺陷密切相关，与重症肌无力发病密切相关，从基因水平揭示重症肌无力的发病机制在基因水平诱导细胞凋亡以抑制自身抗体产生，将为重症肌无力的治疗提供新的手段。     

骨肉瘤发生发展中Caspase-3和Fas的表达及作用

目的:研究Caspase-3和Fas在骨肉瘤组织中的表达,及其在骨肉瘤发生发展中的可能作用。方法:收集中国医科大学附属第一医院1995-05/2002-11骨肉瘤标本38个及骨软骨瘤标本20个。用SP免疫组织化学方法分别检测Caspase-3和Fas在38例骨肉瘤及20例骨软骨瘤中的表达。结果:Caspase-3在骨肉瘤中表达阳性率为53%(20/38),在对照组骨软骨瘤中为85%(17/20),Caspase-3在骨肉瘤组织中的表达明显低于骨软骨瘤(χ2=5.944,P<0.05)。Fas在骨肉瘤中表达阳性率为74%(28/38),在对照组骨软骨瘤中为95%(19/20),Fas在骨肉瘤组织中的表达明显低于骨软骨瘤(χ2=3.874,P<0.05)。骨肉瘤中Caspase-3和Fas的表达呈显著正相关(r=0.391,P<0.05)。结论:Caspase-3和Fas表达减低与骨肉瘤的发生有关,且具有相关性。     

Significance of p53 and bcl-2 immunoexpression in the prognosis of laryngeal squamous cell carcinoma

This study was undertaken to determine whether p53 or bcl-2 immunoexpression has any significance in the prognosis of laryngeal squamous cell carcinoma. Eighty-four cases of laryngeal squamous cell carcinoma at different pathological stages and locations were studied. All patients were treated surgically first, and followed up for 6-96 months (mean, 35 months). p53 and bcl-2 immunoexpression in tumour cells, evaluated by image analysis and histoscore, were found in 71.4% and 46.4% of tumours, respectively. Glottic location was correlated with significantly longer survival than tumours at other locations. Pathological stage and histological grade of tumour were also significant predictors of survival. Neither p53 nor bcl-2 immunoexpression was found to be predictive of post-operative survival. Only transglottic tumours expressed significantly high levels of bcl-2. Both methods used to evaluate p53 and bcl-2 immunoexpression correlated well with each other. This study suggests that immunoexpression of p53 and bcl-2 is not a significant prognostic predictor in laryngeal squamous cell carcinoma.     

P53和c-myc及Bcl-2基因蛋白的表达对子宫平滑肌肿瘤发生与预后的影响

目的:研究P53,c-myc,Bcl-2基因蛋白在子宫平滑肌肿瘤中的表达状况,以探讨上述基因与子宫平滑肌肿瘤发生与预后的关系。方法:根据术后肿瘤病理组织学类型,65例子宫平滑肌肿瘤患者分为平滑肌瘤组、平滑肌交界性肿瘤组、平滑肌肉瘤组。选取20例功能失调性子宫出血患者切除的子宫组织作为正常子宫平滑肌组。应用免疫组织化学染色方法检测肿瘤组织及正常平滑肌组织中P53,c-myc,Bcl-2基因蛋白的表达状况。结果:P53基因蛋白的表达阳性率正常子宫平滑肌组无,子宫平滑肌瘤组为10%,子宫平滑肌交界性肿瘤组为39.3%,子宫平滑肌肉瘤组为85.7%,正常子宫平滑肌组与子宫平滑肌瘤组比较差异无显著性意义(P>0.05),其余各组比较差异均有显著性意义(χ=4.83～22.04,2P<0.01～0.05)。c-myc基因蛋白的表达阳性率正常子宫平滑肌组无,子宫平滑肌瘤组为6.7%,子宫平滑肌交界性肿瘤组为35.7%,子宫平滑肌肉瘤组为71.4%,正常子宫平滑肌组与子宫平滑肌瘤组比较差异无显著性意义(P>0.05),其余各组比较差异均有显著性意义(χ2=3.89～17.53,P<0.01～0.05)。Bcl-2基因蛋白表达阳性率子宫平滑肌瘤组为63.3%,子宫平滑肌交界性肿瘤组为21.4%,子宫平滑肌肉瘤组为14.3%,正常子宫平滑肌组为35%,正常子宫平滑肌组与子宫平滑肌瘤组比较差异有显著性     

食管癌中C-erbB-2,bc1-2,mdr-1的表达与同步放化疗疗效及预后关系的研究

目的：研究食管癌中C-erbB-2，be1-2，mdr-1表达与其同步放化疗疗效及预后的相关性。方法：应用免疫组化技术（S-P法）对187例食管鳞癌进行初治前、治疗后复发或转移不同阶段胃镜活检标本中C-erbB-2，be1-2，mdr-1三种基因蛋白表达的检测。初治均按拟定的统一放化疗方案进行：DF方案化疗同步超分割放疗。结果：187例食管鳞癌细胞中，C-erbB-2，be1-2，mdr-1阳性表达分别为48．7％，55．1％和42．2％，治疗后完全缓解68例（36．4％），部分缓解81例（43、3％），稳定31例（16．6％），进展7例（3、7％）。C-erbB-2阴性者疾病进展时间在24～36个月者38例（39．6％），〈12个月者占19、7％；C-erbB-2阳性者，疾病进展时间〈12个月者比例较高；强阳性者所占比例最高达18／42（42．9％）。C-erbB-2与mdr-1同时阳性表达疾病进展时间〈12个月者比例较高（45．7％）；二者均阴性疾病进展时间在24～36个月所占比例较高（46．2％）。bcl-2强阳性完全缓解占21、3％，阴性完全缓解达50．0％。结论：本实验有希望为食管癌不同阶段制定个体化治疗方案，为观察疗效和预后提供更科学合理的依据。     

姜黄素致HepG-2细胞的凋亡作用及对bcl-2/bax表达的影响

目的:研究姜黄素致人肝癌HepG-2细胞的凋亡作用及bax与bcl-2 mRNA表达的变化,为姜黄素的临床应用提供实验依据.方法:当HepG-2细胞处于对数生长期时,分别加入不同浓度(0、2.5、5、10、20、30 mg/L)的姜黄素处理48 h,MTT试验检测细胞的抑制率;流式细胞仪检测细胞凋亡率;实时荧光定量PCR检测bax和bcl-2mRNA的含量.结果:随着姜黄素浓度的增高,其对HepG-2细胞的抑制作用逐渐增强.当姜黄素浓度为30mg/L时,其抑制率达到(58.23±2.56)%,IC50=25.44 mg/L,而细胞的凋亡率由对照组的(1.1±0.3)%上升到(38.4±1.5)%;bax和bcl-2分别是对照组的(5.00±0.55)倍和(0.28±0.05)倍.结论:姜黄素能抑制HepG-2细胞的生长,诱导细胞凋亡,它的分子机制可能是上调bax基因和下调bcl-2基因的表达.     

MMP-9和bcl-2在骨肉瘤中的表达及其临床意义

目的研究MMP-9和bcl-2在骨肉瘤中的表达及其与临床病理参数的关系,探讨二者在骨肉瘤发生发展中的作用。方法应用免疫组化（SP）对27例骨肉瘤组织和10例正常骨组织中的MMP-9和bcl-2蛋白进行定量的检测,结合临床资料进行分析。结果 1.MMP-9和bcl-2在骨肉瘤中表达的阳性率明显高于正常骨组织P〈0.05）。2.MMP-9b、cl-2在骨肉瘤中表达无相关性（r=-0.209,P〉0.05）。3.MMP-9及bcl-2表达与骨肉瘤病理参数的关系：MMP-9表达与软组织浸润有关（P〈0.05）,与性别、X线分类、组织学类型无关（P〉0.05）,bcl-2与软组织浸润、性别、X线分类、组织学类型均无关（P〉0.05）。结论 MMP-9和bcl-2在骨肉瘤在骨肉瘤发生、发展和转移过程中起着重要的作用,与骨肉瘤的预后有关。     

人绒毛膜促性腺激素对3AO细胞bcl-2和bax表达的影响

目的初步分析人绒毛膜促性腺激素（HCG）对3AO细胞内凋亡相关基因bcl-2和bax在蛋白质和mRNA水平表达的影响,探讨HCG信号在癌细胞增殖凋亡中的作用机制。方法用不同浓度HCG处理卵巢癌3AO细胞72h,采用流式细胞术间接免疫荧光法和实时定量逆转录聚合酶链式反应法分别测定3AO细胞内凋亡蛋白质bcl-2和bax在蛋白质和基因水平的表达。结果HCG不改变3AO细胞bcl-2、bax的表达（P〉0．05）。结论HCG在体外抑制卵巢癌细胞凋亡的作用可能与凋亡基因bcl-2和bax无关。