何首乌中1个新的二苯乙烯苷类化合物

目的：对何首乌Polygonum multiflorum Thunb.干燥块根的70%乙醇提取物进行化学成分研究。方法：采用DM-8型大孔吸附树脂、十八烷基硅烷键合硅胶（ODS）、Sephadex LH-20和制备高效液相色谱等柱色谱方法进行分离纯化,利用质谱和现代波谱学技术对化合物进行结构鉴定。结果：从何首乌70%乙醇提取物中分离得到6个化合物,分别鉴定为(E)-2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷-6′-棕榈酸酯（1）、顺式-大黄素-大黄素甲醚二蒽酮（2）、反式-大黄素-大黄素甲醚二蒽酮（3）、儿茶素（4）、表儿茶素（5）和(E)-2,3,5,4′-四羟基二苯乙烯-2-O-α-L-鼠李糖苷（6）。结论：化合物1为新的二苯乙烯苷类化合物,命名为何首乌辛素。化合物2和3均为首次从何首乌中分离得到。     

何首乌三个栽培种核型比较

目的:分析何首乌Polygonum multiflorum三地栽培种的染色体核型。方法:采用植物染色体常规压片法,结合显微摄影。结果:(1)广西靖西栽培种的核型公式为K(2n)=3X=33=9m 24 sm,核型类型为2A;(2)广东德庆种的核型公式为K(2n)=2X=22=12m 10 sm,核型类型为2B;(3)江西新建种的核型公式为K(2n)=2X=22=6m 14 sm 2st,核型类型为2A。结论:三地栽培的何首乌核型存在差异。     

翠云草中1个新的芳基黄酮

研究翠云草Selaginella uncinata全草的化学成分。采用大孔吸附树脂、硅胶、Sephadex LH-20柱色谱以及高效液相色谱等分离方法,从翠云草75%乙醇提取物中分离得到1个黄酮类化合物,根据理化性质和各种波谱技术鉴定该化合物为8-(4-羧基苯氧基)-5,4'-二羟基-7-甲氧基二氢黄酮(1),该化合物为新的芳基黄酮类化合物,命名为翠云草黄酮G。     

波罗蜜果实中化学成分研究

目的研究桑科波罗蜜属植物波罗蜜Artocarpus heterophyllus果实中的化学成分。方法综合运用硅胶柱色谱、ODS柱色谱、SephadexLH-20凝胶柱色谱以及制备型高效液相色谱等方法进行系统分离,根据化合物的理化性质及其波谱数据鉴定化合物的化学结构。结果从波罗蜜果实的90%乙醇提取物中分离得到了17个化合物,分别鉴定为broussonol E(1)、broussoflavonol F(2)、uralenol(3)、5,7,4′-trihydroxy-3′-(3-methyl but-2-enyl)-3-methoxy flavone(4)、8,2′-diprenylquercetin-3-methylether(5)、8-prenylkaempferol(6)、6-异戊烯基柚皮素(7)、licoflavanone(8)、sigmoidin B(9)、4′,5,7-三羟基-8-异戊烯基黄酮(10)、5,3′-二羟基-7,4′-二甲氧基黄酮(11)、3,5,4′-三羟基-7,3′-二甲氧基黄酮(12)、5,3′,5′-三羟基-7,4′-二甲氧基黄酮(13)、5,7,3′,4...     

不同倍性何首乌中二苯乙烯苷和蒽醌类的含量比较

目的:考察二倍体与三倍体何首乌中二苯乙烯苷、蒽醌类成分的含量差异。方法:采用Inertsil ODS-3C18柱,分别用乙腈-水(25∶75)、甲醇-0.1%磷酸(85∶15)作为流动相,在320 nm和254 nm处检测2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、蒽醌类成分的含量。结果:广东德庆二倍体何首乌中二苯乙烯苷的含量最高,广西靖西三倍体何首乌中结合型蒽醌的比例达到了85%。结论:不同倍性何首乌中化学成分存在差异。     

何首乌药材质量评价研究

目的:采用HPLC特征图谱与主成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡萄糖苷(简称二苯乙烯苷)含量综合评价何首乌药材质量。方法:采用HPLC/UV建立何首乌特征图谱分析方法,应用Diamond C18(4.6 mm×250 mm,5μm)色谱柱,甲醇和水梯度洗脱,检测波长280 nm;采用HPLC测定二苯乙烯苷含量,Waters Novapak C18(3.9 mm×150 mm,5μm)色谱柱,乙腈-水(13∶87)流动相,检测波长320 nm。结果:对十六个批次的何首乌样品进行了分析,建立了何首乌的高效液相特征图谱;各样品HPLC色谱峰检出率、图谱相似度与二苯乙烯苷含量呈正相关。结论:为何首乌的鉴定和质量评价提供了依据。     

蕲艾化学成分研究

目的:对产于湖北蕲春的艾叶进行化学成分分离与结构鉴定。方法:利用大孔树脂、SephadexLH-20、MCI gel CHP-20、制备型HPLC等色谱方法进行分离纯化,运用MS、UV、1 H-NMR、13 C-NMR及二维核磁共振等方法进行结构鉴定。结果:从蕲艾叶中分离到6个黄酮单体化合物,分别为5,7,3′,4′-四羟基二氢黄酮(1)、5,7,3′,4′-四羟基,6-甲氧基黄酮(2)、5,7,4′,5′-四羟基,3′,6-二甲氧基黄酮(3)、芹菜素(4)、木樨草素(5)和槲皮素(6)。结论:化合物1～3均为首次从该植物中分离得到。     

杜仲皮化学成分研究

目的研究杜仲Eucommia ulmoides皮的化学成分。方法采用正相硅胶柱色谱、反向ODS柱色谱、Sephadex LH-20柱色谱、制备HPLC等手段对杜仲皮化学成分进行分离纯化,并根据HR-ESI-MS、NMR等波谱数据进行结构鉴定。结果从杜仲皮95%乙醇提取物中分离得到9个化合物,鉴定为甘草黄酮B(1)、3,5,4′-三羟基-7,3′-二甲氧基黄酮(2)、甘草素(3)、formononetine(4)、(+)-4′-O-methylglabridin(5)、paratocarpin E(6)、龙血素C(7)、threo-1-(4-hydroxy-3-methoxyphenyl)-1-methoxy-2-{4-[1-formyl-(E)-vinyl]-2-methoxyphenoxy}-3-propanol(8)、diospyrosin(9),其中7个黄酮和2个苯丙素类。结论化合物1～9均首次从杜仲属植物中分离得到。     

穿心莲黄酮类化学成分的研究

目的:研究穿心莲Andrographis paniculata地上部分的黄酮类化学成分。方法:采用硅胶s、ephadex LH-20、反相ODS柱色谱、反相HPLC等从穿心莲85%乙醇提取物中分离得到12个黄酮类化合物,并利用理化性质和波谱学分析手段,鉴定了它们的化学结构。结果:分离并鉴定了12个化合物,分别为5-羟基-7,8-二甲氧基黄酮(1),5-羟基-7,8-二甲氧基二氢黄酮(2),5-羟基-7,8,2′,5′-四甲氧基黄酮(3),2′-甲氧基黄芩新素(4),5-羟基-7,8,2′,3′-四甲氧基黄酮(5),5,4′-二羟基-7,8,2′,3′-四甲氧基黄酮(6),二氢黄芩新素(7),5,7,8-三甲氧基二氢黄酮(8),5,2′-二羟基-7,8-二甲氧基黄酮(9),andrographidine C(10),5,7,4′-三羟基黄酮(11),5,7,3′,4′-四羟基黄酮(12)。结论:化合物6为新天然产物,化合物8,11,12为首次从该属植物中分得。     

思茅豆腐柴中的黄酮类化学成分研究

 目的研究民族药物思茅豆腐柴(Premna szemaoensis Pei)中的黄酮类化学成分。方法利用Sephadex LH-20及硅胶等色谱技术进行分离纯化,根据理化性质、光谱数据进行结构鉴定。结果得到7个黄酮类化合物,分别鉴定为5,4'-二羟基- 3,7,3'-三甲氧基黄酮(1),5-羟基-3',4',6,7-四甲氧基黄酮(2),5,4'-二羟基-7-甲氧基黄酮醇(3),5,3'-二羟基-7,4'-二甲氧基黄酮醇(4),3',4',5-三羟基-3,7-二甲氧基黄酮(5),5,7-二羟基-4'-甲氧基黄酮(6),5-羟基-7,3',4'-三甲氧基黄酮醇(7)。结论这7个化合物均为首次从思茅豆腐柴中分离得到。     

Isolation and structure of a 20,21-epoxybufenolide series from "Ch'an Su"

Steroid bufenolides resulting from epoxidation of the 17beta-2-pyrone ring of bufadienolides are rare. Five 20,21-epoxybufenolides, namely, 20S,21-epoxyresibufogenin (1), 20R,21-epoxyresibufogenin (2), 3-O-formyl-20S,21-epoxyresibufogenin (3), 3-O-formyl-20R,21-epoxyresibufogenin (4), and 3-oxo-20S,21-epoxyresibufogenin (5), were isolated from the Chinese toad skin extract drug Ch'an Su. The structures were elucidated by spectroscopic and chemical methods. The configuration at C-20 was assigned by the analysis of difference NOE spectra. The cancer cell (KB and MH-60) growth inhibition by the new 20,21-epoxybufenolides was examined, and 20,21-epoxides 1, 2, and 5 were found to significantly inhibit the leukemia MH-60 cell line.     

Triterpenoids and steroids from the fruits of Melia toosendan and their cytotoxic effects on two human cancer cell lines

Ten new triterpenoids, named meliasenins I-R (1-10), one new steroid (11), and 11 related known compounds (12-22) were isolated from fruits of Melia toosendan. The structures of the new compounds were established on the basis of spectroscopic methods, including 2D NMR techniques and mass spectrometry. The relative configuration of 1, (20R*,23E)-25-hydroperoxyeupha-7,23-diene-3β,16β-diol (meliasenin I), was confirmed by single-crystal X-ray diffraction analysis. All isolated triterpenoids (1-10, 12-15) and two steroids (11, 20) were tested for their cytotoxicity against U20S human osteosarcoma and MCF-7 human breast cancer cells using the MTT assay, and some of them were significantly cytotoxic (IC(50) <10 μg/mL). The insecticidal properties of compounds 1-15 and 20 were also briefly evaluated.     

Fusarium sacchari转化三七茎叶皂苷中新化合物的分离和鉴定

目的 利用甘蔗镰孢Fusarium sacchari对三七茎叶皂苷进行生物转化,以发现新的稀有抗肿瘤活性成分.方法 转化产物通过硅胶、凝胶及液相色谱进行分离,得到化合物结构经波谱鉴定.结果 分离得到了1个单体化合物,命名为三七皂苷-LZ(notoginsenoside-LZ),鉴定其化学结构为20(S)-3β-hydroxy-12β,23-epoxy-dammar-24-ene-20-O-β-D-xylopyranosyl(1→6)-β-D-glucopyranoside.结论 三七皂苷-LZ(notoginsenoside-LZ)为一新化合物.     

Microbial transformation of 20(S)-protopanaxatriol by Mucor spinosus

Microbial biotransformation of 20(S)-protopanaxatriol (1) by the fungus Mucor spinosus (AS 3.3450) yielded four new metabolites, the structures of which were determined to be 12-oxo-15alpha-hydroxyl-20(S)-protopanaxatriol (2), 27-hydroxyl-20(S)-protopanaxatriol (3), 12-oxo-26-hydroxyl-20(S)-protopanaxatriol (4), and 12-oxo-27-hydroxyl-20(S)-protopanaxatriol (5), respectively, on the basis of their chemical and spectroscopic data. All the new metabolites, as well as the substrate, had significant cytotoxic effects on HL-60 cells (human leukemia cells).     

Three new triterpenoids from Peganum nigellastrum

Three new triterpenoids, 3alpha,27-dihydroxylup-20(29)-en-28-oic acid methyl ester, 3alpha-acetoxy-27-hydroxylup-20(29)-en-28-oic acid methyl ester, and 3alpha-acetoxyolean-12-ene-27,28-dioic acid 28-methyl ester, were isolated from the roots of Peganum nigellastrum along with four known lupene-type triterpenoids. The structures of the new triterpenoids were determined by NMR spectroscopic means. The new triterpene, 3alpha, 27-dihydroxylup-20(29)-en-28-oic acid methyl ester is a DNA topoisomerase II inhibitor (IC(50) = 8.9 microM/mL).     

New vibsane diterpenes and lupane triterpenes from Viburnum odoratissimum

Bioassay-directed fractionation of the methanolic extract of the leaves and flowers of Viburnum odoratissimum resulted in the isolation of two new diterpenes, vibsanol A (1) and vibsanol B (2), together with two new triterpenoids, 6beta-hydroxylup-20(29)-en-3-oxo-27,28-dioic acid (3) and 6alpha-hydroxylup-20(29)-en-3-oxo-27,28-dioic acid (4). In addition, the known terpenoids vibsanins B and E, and 6alpha-hydroxylup-20(29)-en-3-oxo-28-oic acid, were also isolated. The structures of the new compounds were established by chemical and spectroscopic means. Vibsanol A (1) and compound 3 exhibited significant cytotoxicity against human gastric (NUGC) tumor cells.     

Taraxastane-type triterpenes from the aerial roots of Ficus microcarpa

Seven new taraxastane-type triterpenes-20-taraxastene-3beta, 22alpha-diol (1), 3beta-acetoxy-20-taraxasten-22alpha-ol (2), 3beta-acetoxy-22alpha-methoxy-20-taraxastene (3), 3beta-acetoxy-20alpha,21alpha-epoxytaraxastan-22alp ha-ol (4), 3beta-acetoxy-19alpha-methoxy-20-taraxastene (5), 3beta-acetoxy-19alpha-hydroperoxy-20-taraxastene (6), 3beta-acetoxy-20alpha,21alpha-epoxytaraxastane (7)-were isolated from the aerial roots of Ficus microcarpa. Their structures were elucidated by spectroscopic and chemical methods.     

Oxygenated lanostane-type triterpenoids from the fungus ganodermalucidum

Two new triterpenoids, 20(21)-dehydrolucidenic acid A (1) and methyl 20(21)-dehydrolucidenate A (2), and five new 20-hydroxylucidenic acids, 20-hydroxylucidenic acid D(2) (3), 20-hydroxylucidenic acid F (4), 20-hydroxylucidenic acid E(2) (5), 20-hydroxylucidenic acid N (6), and 20-hydroxylucidenic acid P (7), were isolated from the fruiting body of the fungus Ganoderma ludicum, and their structures were established on the basis of spectroscopic methods.     

New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture

Three new monoterpenoid indole alkaloids, 19(S),20(R)-dihydroperaksine (1), 19(S),20(R)-dihydroperaksine-17-al (2), and 10-hydroxy-19(S),20(R)-dihydroperaksine (3), along with 16 known alkaloids 4-19 were isolated from hairy root culture of Rauvolfia serpentina, and their structures were elucidated by 1D and 2D NMR analyses. Taking into account the stereochemistry of the new alkaloids and results of preliminary enzymatical studies, the putative biosynthetical relationships between the novel alkaloids are discussed.     

Mycalamide D, a new cytotoxic amide from the New Zealand marine sponge Mycale species

A new mycalamide, mycalamide D (3), has been isolated from the New Zealand marine sponge Mycale sp. This new metabolite, in which the C13-O-methyl group of mycalamide A (1) is replaced by a hydrogen atom, was found to be cytotoxic to a range of mammalian cell lines, with a potency approximately 20-fold less than that of 1.