芒柄花素磺酸钠通过MAPKS信号通路缓解OVA诱导哮喘小鼠肺部炎症

[目的] 观察芒柄花素磺酸钠对卵清蛋白（OVA）诱导哮喘小鼠的保护作用及其机制。[方法] 购买SPF级C57BL/6小鼠60只,并随机分6组,每组10只,具体组别如下：空白对照组（CON组）、模型组（OVA组）、芒柄花素磺酸钠低剂量（MBHS-L组）、芒柄花素磺酸钠中剂量（MBHS-M组）、芒柄花素磺酸钠高剂量（MBHS-H组）和地塞米松组（DEX组）,使用OVA诱导建立小鼠哮喘模型。实验主要评价小鼠哮喘症状并进行评分、计量支气管肺泡灌洗液（BALF）中炎症细胞数量（Diff-Quick染色法）、镜下观察肺组织的病理学变化[苏木精-伊红（HE）染色法]、分析BALF中超氧化物歧化酶（SOD）活性和肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、白细胞介素6（IL-6）和丙二醛（MDA）水平（ELISA法和化学发光法）、分析肺组织p-ERK1/2,p-JNK和p-p38MAPK蛋白表达水平（免疫荧光法和Western blot法）。[结果] 与OVA组比较,哮喘症状评分、BALF炎症细胞数量、TNF-α、IL-1β和MDA水平以及p-ERK1/2,p-JNK和p-p38MAPK蛋白表达水平在MBHS组和DEX组均明显降低（P＜0.05）,而SOD的活性明显升高（P＜0.05）,肺组织病理学变化明显改善。[结论] 芒柄花素磺酸钠可能通过MAPKS信号通路缓解OVA诱导哮喘小鼠的肺部炎症。     

金线莲提取物对脂多糖诱导的小鼠急性肺损伤的保护作用及机制研究

目的观察金线莲提取物对脂多糖（LPS）诱导的急性肺损伤（ALI）模型小鼠的疗效并探讨其作用机制。方法60只健康ICR小鼠随机分为正常组、模型组、地塞米松组和金线莲低、中、高剂量组。地塞米松组给予5.0mg/kg地塞米松溶液腹腔注射,金线莲低、中、高剂量组分别给予1.3、2.6、5.2g/kg的金线莲提取物灌胃,对照组和模型组给予0.9%氯化钠溶液灌胃,连续给药2d。第3天对照组给予0.9%氯化钠溶液气管滴注,其余各组均给予5.0mg/kgLPS构建ALI模型。造模后各组再给药1次。12h后颈椎脱臼处死小鼠,获取肺组织及肺泡灌洗液。HE染色观察肺组织病理学变化,测定肺湿干比,ELISA法测定肺泡灌洗液中IL-6、单核细胞趋化蛋白-1（MCP-1）、IL-12/p70、IFN-γ、TNF-α和IL-10的水平,免疫组化法检测肺组织中髓过氧化物酶（MPO）、磷酸化细胞外信号调节激酶（p-ERK）、人核因子κB抑制蛋白α（IκBα）和磷酸化人核因子κB抑制蛋白α（p-IκBα）的蛋白表达水平,Westernblot法检测肺组织中细胞外信号调节激酶（ERK）、p-ERK、c-Jun氨基末端激酶（JNK）、磷酸化c-Jun氨基末端激酶（p-JNK）、p38、磷酸化p38（p-p38）、腺苷酸-活化蛋白激酶α（AMPKα）、磷酸化腺苷酸-活化蛋白激酶α（p-AMPKα）、MPO蛋白相对表达量。结果与对照组相比,模型组小鼠肺组织出现炎性病理变化,肺泡灌洗液中IL-6、MCP-1、IL-12/p70、IFN-γ、TNF-α和IL-10水平均显著升高（均P＜0.01）。与模型组相比,金线莲高剂量组肺组织湿干比明显降低（P＜0.01）,肺泡灌洗液中IL-6、MCP-1、IL-12/p70、IFN-γ、TNF-α和IL-10水平均显著降低（均P＜0.05）,肺组织MPO、p-ERK、p-IκBα蛋白表达水平均显著降低（均P＜0.05）,p-ERK、p-JNK、p-p38、p-AMPKα、MPO蛋白相对表达量均显著降低（均P＜0.05）。结论一定剂量金线莲提取物可有效保护LPS所致ALI,其作用机制可能与调节丝裂原活化蛋白激酶信号通路、减轻炎症反应有关。     

没食子酸通过NF-κB通路对缺氧诱导的 BV2小胶质细胞损伤的保护作用机制

目的:建立BV2细胞缺氧损伤模型,研究没食子酸通过NF-κB通路对缺氧诱导的BV2小胶质细胞损伤的保护作用机制。方法:取BV2小胶质细胞分为空白组、模型组、低剂量、中剂量及高剂量组,通过三气培养箱建立BV2小胶质细胞缺氧损伤模型,低剂量、中剂量及高剂量组分别给予1.7 g/mL、3.4 g/mL、8.5 g/mL没食子酸进行干预。采用CCK-8法检测没食子酸对缺氧的BV2细胞的保护作用,使用试剂盒检测上清中乳酸脱氢酶(LDH)、细胞中超氧化物歧化酶(SOD)活力、丙二醛(MDA)和活性氧(ROS)的含量,ELISA检测上清中炎症因子表达水平(TNF-α、IL-1β、IL-6),Western blot检测BV2细胞内p-IKK/IKK、p-IκB/IκB和pNF-κB p65/NF-κB p65蛋白的表达水平以及NF-κB p65的核位移。结果:与空白对照组相比,缺氧可造成BV2细胞培养基上清液LDH的活力增加和炎症因子(TNF-α、IL-1β、IL-6)的释放,细胞中SOD活力降低,MDA和ROS的水平升高,NF-κB通路(p-IKK/IKK、p-IκB/IκB和p-NF-κB p6...     

雷公藤甲素对哮喘小鼠白介素8及白介素10表达的影响

目的研究雷公藤甲素对哮喘小鼠的治疗作用及对白介素8（IL-8）及白介素10（IL-10）表达的影响。方法 60只小鼠,随机分6组,每组各10只。分别为正常对照组（A）、哮喘组（B）、地塞米松治疗组（C）及不同剂量雷公藤甲素治疗组（D、E、F）,建立小鼠卵蛋白哮喘模型,用ELISA测定肺组织匀浆及气管肺泡灌洗液（BALF）中IL-8、IL-10表达水平。结果哮喘组小鼠肺组织匀浆及BALF中IL-8表达均高于正常对照组（P〈0.01）,哮喘组小鼠肺组织匀浆及BALF中IL-10表达均低于正常对照组（P〈0.01）,雷公藤甲素治疗组及地塞米松组IL-8低于哮喘组（P〈0.01）,雷公藤甲素治疗组及地塞米松组IL-10高于哮喘组（P〈0.01）。结论雷公藤甲素对哮喘小鼠有明显治疗作用,可抑制IL-8表达及提高IL-10的表达。     

不同剂量甲泼尼龙对肺炎支原体肺炎模型小鼠肺组织TLR4/NF-κB通路的影响

目的 探究不同剂量甲泼尼龙对肺炎支原体肺炎模型小鼠肺组织Toll样受体4（Toll-like receptor 4,TLR4）/核因子κB（nuclear factor κB ,NF-κB）通路的影响。 方法 选取40只SD健康雄性小鼠,随机选取10只为正常小鼠组,其余30只建立肺炎支原体肺炎小鼠模型,建模成功后随机选取10只做模型组,其余20只分别为低剂量干预组、高剂量干预组,其中低剂量干预组小鼠以低剂量甲泼尼龙进行注射,高剂量干预组小鼠以高剂量甲泼尼龙进行注射。比较各组间TLR4、NF-κB表达,免疫组织化学法检测TLR4、NF-κB水平,酶联免疫法检测及肿瘤坏死因子α（tumor necrosis factor-α,TNF-α）、白细胞介素1β（interleukin-1β,IL-1β）水平,黄嘌呤氧化酶法检测超氧化物歧化酶（superoxide dismutase,SOD）,硫代巴比妥酸法检测丙二醛（malondialdehyde,MDA）。 结果 相比正常小鼠组,模型组、低剂量干预组与高剂量干预组TLR4、NF-κB表达均较高,低剂量干预组与高剂量干预组TLR4、NF-κB表达低于模型组,且高剂量干预组TLR4、NF-κB表达低于低剂量干预组（P＜0.05）;相比正常小鼠组,模型组、低剂量干预组与高剂量干预组TLR4、NF-κB水平均较高,低剂量干预组与高剂量干预组TLR4、NF-κB水平低于模型组,且高剂量干预组TLR4、NF-κB水平低于低剂量干预组（P＜0.05）;相比正常小鼠组,模型组、低剂量干预组与高剂量干预组TNF-α、IL-1β水平均较高,低剂量干预组与高剂量干预组TNF-α、IL-1β水平低于模型组,且高剂量干预组TNF-α、IL-1β水平低于低剂量干预组（P＜0.05）;相比正常小鼠组,模型组、低剂量干预组与高剂量干预组MDA水平较高、SOD水平均较低,与模型组相比,低剂量干预组与高剂量干预组MDA水平较低、SOD水平较高,且高剂量干预组MDA水平低于、SOD水平高于低剂量干预组（P＜0.05）。 结论 在肺炎支原体肺炎模型小鼠中,应用甲泼尼龙能够显著改善出现的肺组织损伤,抑制TLR4/NF-κB通路表达,降低炎症反应程度,改善氧化应激,且高剂量效果较为显著。     

川陈皮素对阿尔茨海默病小鼠海马区神经细胞的修复作用及对p38MAPK/NF-κB通路的影响

目的 探讨川陈皮素对阿尔茨海默病(Alzheimer's disease,AD)小鼠海马区神经细胞的修复作用以及其对p38细胞丝裂原活化蛋白激酶(p38 mitogen-activated protein kinase,p38MAPK)/核因子-κB(nuclear factor-κB,NF-κB)通路的影响.方法 将80只小鼠随机均分为对照组、模型组、川陈皮素组、激活剂组、川陈皮素+激活剂组,用不同药物干预后,检测各组小鼠学习与记忆能力、方位识别能力;苏木精-伊红(hematoxylin-eosin,HE)染色法观察海马CA1区神经细胞病理学变化;末端脱氧核苷酸转移酶介导的dUTP原位缺口标记技术(terminal deoxynucleotidyl transferase TdT-mediated biotin dUTP nick end-labeling,TUNEL)染色观察海马神经细胞凋亡;Western blot检测脑组织p38MAPK、p-p38MAPK、NF-κB p65、p-NF-κB p65蛋白表达.结果 与对照组比较,模型组、川陈皮素组、激活剂组、川陈皮素+激活剂组进入安全区所需训练次数增多,海马CA1区神经细胞凋亡率、p-p38MAPK、p-NF-κB p65蛋白相对表达水平升高,且激活剂组>模型组>川陈皮素+激活剂组>川陈皮素组,停止训练后进入安全区潜伏时间缩短,训练3、7天小鼠方位识别正确率降低,且川陈皮素组>川陈皮素+激活剂组>模型组>激活剂组(P<0.05).HE染色结果显示:对照组锥体细胞多且排列整齐,胞体及核膜完整,胞核大且圆,染色均匀;模型组和激活剂组神经细胞明显减少且皱缩,排列紊乱,胞质浓染,胞核着色较深,核仁消失;川陈皮素+激活剂组和川陈皮素组病理学变化有不同程度的改善.结论 川陈皮素能够修复AD小鼠海马区神经细胞,可能是通过抑制p38MAPK/NF-κB通路发挥作用.     

脊髓损伤小鼠中甘草苷通过抑制MAPK通路抑制炎症和神经元凋亡

目的 研究甘草苷对脊髓损伤小鼠中炎症和神经元凋亡的影响。方法 将48只C57BL/6小鼠随机均分为假手术组（Sham组）、脊髓损伤模型组（SCI组）、低剂量甘草苷组（LQ-L组）、高剂量甘草苷组（LQ-H组）,每组12只。采用脊髓打击器撞击脊髓建立脊髓损伤模型,LQ-L组和LQ-H组分别予以低剂量（30 mg/kg）和高剂量（60 mg/kg）甘草苷灌胃。采用后肢运动功能评分（BMS）评估小鼠运动功能。取脊髓组织,HE染色观察病变面积,比色法检测髓过氧化物酶活性,TUNEL检测细胞凋亡,Nissl染色观察神经元丢失,免疫荧光双染检测caspase 3阳性神经元数量,Western blotting检测MAPK通路蛋白表达。结果 脊髓损伤明显降低了小鼠运动功能,促进了脊髓炎症、病变面积、神经元凋亡及p-ERK、p-p38 MAPK、p-JNK蛋白表达（P <0.05）。LQ-H组术后第14、21天,脊髓损伤小鼠运动功能明显提高（P <0.05）。LQ-L组和LQ-H组脊髓炎症、病变面积、神经元凋亡及p-ERK、p-p38 MAPK、p-JNK蛋白表达均明显抑制（P <0...     

大麻素受体2在脓毒症大鼠急性肺损伤中的作用及其机制

目的 探讨大麻素受体2 (CB2R)对脓毒症大鼠急性肺损伤的作用及其机制。方法 将SD大鼠随机分为对照组、模型组、CB2R激动剂（JWH133）组和CB2R拮抗剂（AM630）组。模型组腹腔注射脂多糖（LPS）构建脓毒症模型;JWH133组和AM630组在注射LPS前30 min注射JWH133和AM630。6 h后光镜和电镜下观察肺组织结构改变;检测肺组织含水率以及支气管肺泡灌洗液（BALF）中TNF-α、IL-1β的含量;实时定量PCR检测肺组织ERK1/2及NF-κB p65 mRNA的表达;Western blotting检测肺组织TNF-α、IL-1β、ERK、p-ERK、NF-κB p65、p-NF-κBp65的蛋白表达。结果 与对照组比较,模型组肺泡结构破坏,大量炎症细胞浸润,超微结构破坏;肺组织含水率升高;BALF中TNF-α、IL-1β水平升高（P <0.05）;肺组织ERK1/2、NF-κB p65 mRNA表达及TNF-α、IL-1β、p-ERK1/2、p-NF-κB p65蛋白表达水平升高（P <0.05）。与模型组相比,JWH133组病理改变及超微...     

Maresin-1对哮喘小鼠肺部炎症及MAPK信号通路的影响

目的探讨Maresin-1(MaR1)对哮喘小鼠肺部炎症及MAPK信号通路的影响。方法将24只雌性BALB/c小鼠随机分成正常组、哮喘模型组、MaR1组及地塞米松组。采用卵清蛋白(OVA)联合氢氧化铝建立哮喘模型,然后分别予MaR1及地塞米松处理哮喘小鼠。收集各组小鼠的血清、支气管肺泡灌洗液(BALF)及肺组织作进一步分析。小鼠肺组织病理变化采用苏木精–伊红及过碘酸希夫染色检测;瑞式–姬姆萨染色法分类BALF中各炎症细胞的比例;酶联免疫吸附试验检测血清、BALF中Th2相关的炎症因子白细胞介素(IL)-4、IL-5、IL-13和IgE;蛋白印迹法检测肺组织中p-p38、p-JNK的蛋白含量。结果与正常组相比,哮喘模型组肺组织炎症反应增强、气道杯状细胞数量明显增多;BALF中各类炎症细胞数量明显增加;BALF中的IL-4、IL-5、IL-13及血清中的IgE、OVA-specific-IgE水平显著升高;肺组织中p-p38、p-JNK的蛋白含量明显增加(均P0.05)。与哮喘模型组相比,MaR1和地塞米松组均减轻了哮喘小鼠肺组织中的炎症反应及黏液的分泌,减少了BALF中炎症细胞的数量,降低了BALF中Th2相关的炎症因子和血清中IgE的水平,同时减少了肺组织中p-p38、p-JNK蛋白的表达(均P0.05)。结论 MaR1能够抑制Th2相关的炎症因子和IgE的产生及释放,有效地减轻哮喘小鼠肺组织的炎症反应和黏液生成,作用效果与地塞米松相近,其作用机制可能与下调MAPKs信号通路的表达有关。     

替米沙坦对脂多糖诱导3T3-L1脂肪细胞炎症通路的影响

目的 研究替米沙坦对脂多糖诱导3T1-L1脂肪细胞炎症通路的影响,探讨替米沙坦的抗炎机制.方法将3T3-L1脂肪细胞诱导至90%成熟,按随机数字表法分为6组:对照组、LPS组、LPS+替米沙坦(0.01、0.1、1和10μg/ml)组(n=18),向对照组细胞中加DMSO,LPS+替米沙坦组细胞中加入不同浓度替米沙坦,孵育2h后,LPS组和LPS+替米沙坦组再加入脂多糖(1 μg/ml),孵育1h,Western免疫印记法比较各组核蛋白NF-κBp65、总蛋白p-IκBα、IκBα、p-ERK1/2、ERK1/2、P-p38MAPK、p38 MAPK的表达水平.结果 脂多糖刺激后IκBα磷酸化蛋白表达增强(P＜0.05),细胞核内NF-κBp65蛋白表达增多(P＜0.05),MAPK通路相关蛋白ERK1/2磷酸化和p38MAPK磷酸化水平明显增强(P＜0.05),替米沙坦可抑制IκBα磷酸化和NF-κBp65核转位,p-IκBα蛋白表达和核蛋白NF-κBp65表达均降低,大剂量替米沙坦(10 μg/ml)作用时该作用明显(P＜0.05).替米沙坦干预后,p-ERK1/2和p-p38MAPK蛋白表达也受到明显抑制(P＜0.05),大剂量(10μg/ml)时作用明显(P＜0.05).结论 在3T3-L1脂肪细胞中,替米沙坦可以抑制NF-κB通路中IκBα蛋白磷酸化、NF-κBp65蛋白核转位以及MAPK通路中ERK1/2蛋白和p38MAPK蛋白的磷酸化,从而发挥抗炎作用.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.