解脲支原体和沙眼衣原体感染对女性不孕的影响

目的 探讨女性不孕症与解脲支原体(Uu)、沙眼衣原体(CT)感染的关系.方法 分别采用培养法和ELISA方法检测175例不孕妇女Uu、CT感染率与120例健康生育妇女(对照组)Uu、CT感染率.结果 不孕组Uu感染阳性率为46.3%,CT感染阳性率为16.0%,Uu、CT混合感染(Uu+CT)阳性率为11.4%;对照组Uu感染率为10.8%,CT感染率为2.5%,Uu、CT混合感染(Uu+CT)阳性率2.5%.两组间差异有统计学意义(P<0.05).结论 女性生殖道Uu感染是女性不孕症的生殖道感染主要因素.     

沙眼衣原体和解脲支原体感染与不孕关系的临床分析

目的：探讨生殖道沙眼衣原体（CT）和解脲支原体（UU）对不孕的影响.方法：CT采用金标法.UU采用培养及药敏鉴定.对128例原发不孕,106例继发不孕共234例不孕症妇女（不孕症组）进行CT、UU检测,并与100例正常妇女进行对照,阳性患者夫妇同时治疗.结果：不孕症组CT和UU阳性率分别为30.8%和35.4%,对照组CT、UU阳性率分别为8%和13%,两组间差异均有极显著性（P＜0.01）,而原发不孕与继发不孕检出率比较差异无显著性（P＞0.05）.不孕症组宫颈糜烂患者CT、UU感染率分别为45.1%和37.8%,明显高于宫颈光滑患者,差异有显著性（P＜0.01）,采用药敏鉴定后敏感药物夫妇双方同时治疗1疗程,CT、UU治愈率可达89%及82.6%.结论：生殖道CT、UU感染是引起女性不孕的原因之一,对不孕夫妇进行CT、UU常规检查并及时治疗非常必要.     

沙眼衣原体感染与输卵管性不孕的关系探讨

本文通过对不孕患者的宫颈分泌物、子宫内膜、腹腔液进行沙眼衣原体CT-DNA检测,以探讨生殖道沙眼衣原体(CT)感染与输卵管性不孕的关系。1对象和方法1.1对象选择我院2003-10~2005-11经腹腔镜及病理学检查除外生殖器结核、子宫内膜异位症所致的不孕患者110例,分别取其宫颈分泌物、子宫内膜和腹腔液,进行CT-DNA检验。其中10例3个部位阳性,24例2个部位阳性18例1个部位阳性。1个或1个以上部位CT-DNA阳性(阳性组)52例;3个部位CT-DNA均阴性者(阴性组)58例。两组患者在年龄、不孕年限、职业、原发不孕与继发不孕构成比、宫腔操作史等方面比…     

不孕不育699例衣原体支原体感染情况分析

目的：分析不孕不育患者中支原体衣原体感染情况。方法：对699例不孕不育患者尿道（宫颈）分泌物、前列腺液、精液等进行支原体衣原体检测及药敏试验。结果：本组共检出解脲支原体（UU）、衣原体（CT）、人型支原体（MH）177例。药物以阿奇霉素、交沙霉素较敏感，随访到34对夫妇于治疗后6～18个月怀孕。结论：不孕不育患者中支原体衣原体感染率高，敏感抗生素治疗有效。     

淋病患者沙眼衣原体和解脲支原体混合感染分析

近年来，各种性病病原体的混合感染情况星上升趋势。对于淋病患，已由原来单一的淋病奈瑟菌感染而发展到沙眼衣原体(Ct)和／或解脲支原体(Uu)的混合感染。对我院2003-01～2003—12淋病79例患沙眼衣原体检测和解脲支原体培养，总结分析如下。     

沙眼衣原体和解脲支原体感染与不孕关系及单剂量阿奇霉素疗效观察

1　对象和方法1 .1　对象　 1 999- 0 1～ 2 0 0 2 - 0 4我站女性不孕 1 80例 (原发不孕1 0 0例和继发不孕 80例 )为观察组 ,年龄 2 4～ 37岁 ,平均 30 .6 5岁 ,不育年限 2～ 1 0 a。同期已生育健康妇女 83例为对照组 ,年龄 2 4～ 35岁 ,平均 30 .1岁。1 .2　方法　用消毒棉试子取受检者宫颈分泌物 ,运用聚合酶链反应 (PCR)技术行沙眼衣原体 (CT)、解脲支原体 (UU)检测 ,PCR试剂盒由上海复星实业股份有限公司生产。 CT、UU阳性者 ,给予阿奇霉素胶囊 (每粒 2 5 0 mg) 1 g,单剂量饭前 1 h或饭后 2 h1次服用。停药 2周后复查 ,包括记录其…     

解脲支原体感染与不育分析

对我院近3a来收治的不育症中由输卵管堵塞所致不育的患者的解脲支原体（uu）感染情况及治疗效果与并选择非输卵管原因所致不育的不育症患者uu感染情况及治疗效果作对照，现总结如下。1对象和方法1．1对象A组为因输卵管堵塞所致不育的患者286例，B组为非输卵管原因所致的不育患者286例。全部病例，婚后2～13a性生活正常，A组中原发不孕39例，继发不育247例（其中有死胎及自然流产史者85例，人工流产史94例，有自然分娩史68例）。B组中原发不孕158例，继发不育128例（其中有死胎及自然流产史者43例，人工流产史52例，有自然分娩史33例）。     

沙眼衣原体感染与输卵管性不孕的相关性分析

[目的]探讨沙眼衣原体（Ct）感染与输卵管性不孕的关系。[方法]本院输卵管不孕患者379例[其中原发不孕125例（A组）,继发不孕组254例（B组）],夫妇双方均行Ct检测,并以92对健康已育夫妇为对照组。[结果]A组女方感染14例,占11.2%,男方感染24例,占19.2%,双方同时感染13例,占10.2%,总感染率为40.8%;B组女方感染54例,占21.3%,男方感染80例,占31.4%,双方同时感染42例,占16.5%,总感染率为69.3%。B组总感染率明显高于A组（P〈0.05）;两组与对照组相比均有显著性差异（P〈0.001）。[结论]Ct感染是引起输卵管性不孕,尤其是继发不孕的主要病因,建议将Ct的检测作为不孕症的常规项目之一。     

沙眼衣原体和解脲支原体感染与不孕

目的探讨沙眼衣原体（ＣＴ）、解脲支原体（ＵＵ）的生殖道感染与不孕关系。方法采用聚合酶链反应技术（ＰＣＲ）检测沙眼衣原体和解脲支原体的ＤＮＡ。结果不孕组８８例中，ＣＴ阳性率２５０％，ＵＵ阳性率６２５％，对照组１４１例中，ＣＴ阳性率１１３４％，ＵＵ阳性率２８４％，ＣＴ和ＵＵ的χ２值分别为７０３和２６０１，Ｐ＜００１，有显著性差异。结论沙眼衣原体和解脲体支原体的生殖道感染是不孕的重要因素之一。     

解脲支原体感染与自然流产的相关性分析

目的 探讨生殖道解脲支原体(UU)感染与习惯性流产的关系.方法 采用分离培养法分别对120例自然流产患者(研究组)和120例早孕要求终止妊娠妇女(对照组)进行宫颈分泌物UU培养,并对两组刮宫后的绒毛及蜕膜组织送病理检查.结果 研究组宫颈分泌物UU培养阳性(76例,63.33%)明显高于对照组(23例,19.17%),两组比较差异有统计学意义(P<0.01);研究组71例(59.17%)发生绒毛膜炎,而对照组仅有27例(22.50%),两组比较差异有统计学意义(P<0.01).结论 解脲支原体感染与习惯性流产有相关性,是引起自然流产的主要原因之一.     

3199例妊娠女性沙眼衣原体感染的检测分析

目的了解无锡市不良妊娠女性沙眼衣原体(CT)的感染状况及其与流产的关系。方法选取2013年1月至2014年12月在该院就诊的妊娠女性3 199例,分为稽留流产组(2 633例)、不完全流产组(265例)、难免流产组(137例),先兆流产组(104例),对照组(60例,均为人工流产),采用荧光定量聚合酶链式反应(FQ-PCR)技术进行CT检测。结果稽留流产组、不完全流产组、难免流产组及先兆流产组的CT阳性率分别为5.62%、4.53%、5.11%和4.81%,均高于对照组阳性率(3.33%),但差异均无统计学意义(P0.05)。16~21岁和21~26岁妊娠女性CT阳性率最高,分别为7.06%和6.20%,但各年龄段CT阳性率比较差异无统计学意义(P0.05)。结论在妊娠女性中自然流产相对人工流产有较高的CT感染率,并且年轻女性CT感染率较高。     

316例不孕症妇女解脲支原体和沙眼衣原体检测结果分析

目的了解不孕症妇女解脲支原体（Uu）和沙眼衣原体（CT）的感染状况及其与不孕症的关系。方法对316例患不孕症的妇女（不孕组）和250例正常生育的妇女（对照组）进行宫颈分泌物的uu和cT检测,比较两组妇女Uu与cT感染率差异,并分析原发性不孕症与继发性不孕症妇女感染率的差异。结果不孕组UU、CT感染检出率分别为30．7％、8．9％,明显高于对照组的9．2％和0．8％（P〈o．05）;原发性不孕妇女宫颈分泌物UU、CT感染检出率分别为11．9％、3．7％,而继发性不孕妇女宫颈分泌物UU、CT感染捡出率分别为40．6％、n．6％,UU感染检出率明显高于原发性不孕妇女（P〈O．05）,CT感染检出率明显高于原发不孕妇女（P〈O．05）。结论Uu和CT与妇女不孕密切相关,建议临床将UU、CT作为女性不孕就诊患者的常规检查项目。     

PCR detection and differentiation of Chlamydia pneumoniae, Chlamydia psittaci and Chlamydia trachomatis.

A PCR-based system was developed for the detection and differentiation of Chlamydia trachomatis, Chlamydia psittaci and Chlamydia pneumoniae. A conserved 145 bp fragment of the chlamydial omp1 gene was amplified from all three species. The three species were then differentiated from each other by digestion of this PCR product with restriction enzymes Eco RI and either Hind III or Pst I. The system was shown to work for two strains of C. pneumoniae, 11 strains of C. psittaci and 10 serovars of C. trachomatis, and had a sensitivity of less than 10 chlamydial elementary bodies. This method was also applicable to the detection of C. trachomatis in conjunctival and nasopharyngeal swabs.     

沙眼衣原体、支原体感染的不孕症抗生素治疗后临床观察

目的 :探讨沙眼衣原体 (CT)、支原体感染不孕症夫妇系统抗生素治疗后的临床效果。方法 :对CT ,溶脲脲原体 (Uu)和人型支原体 (Mh)感染的原发不孕和继发不孕症 112例进行系统的夫妻双方抗生素治疗及临床随访。结果 :抗生素治疗后CT、Uu和Mh感染转阴率为 77.7% (87/ 112 ) ,不育丈夫的精液常规明显好转 (P <0 .0 1) ,87例治疗后感染转阴的不孕症患者 1年内有 39例妊娠 ,妊娠率为 4 4 .8% (39/ 87) ,妊娠成功率为 84 .6 % (33/ 39)。 4例患者再次感染CT、Uu和Mh而妊娠失败 ,2例发生宫外孕 ,妊娠失败率为 15 .4 % (6 / 39)。结论 :CT、Uu和Mh感染的不育不孕症抗生素系统治疗是有效的 ,对妊娠妇女有必要进行妊娠早期的CT、Uu和Mh感染监测和再次感染治疗。     

呼吸道感染患儿肺炎支原体和沙眼衣原体检测结果分析

目的了解呼吸道感染患儿肺炎支原体(MP)、沙眼衣原体(CT)的检出情况。方法应用荧光定量聚合酶链反应法对1 393例呼吸道感染患儿同时进行MP、CT检测。结果 MP、CT总检出率为30.4%,其中MP为21.8%,CT为9.3%,MP合并CT为0.65%。在季节分布上,夏季MP和CT检出率最高。在年龄分布上,大于或等于3岁组的患儿MP的检出率要明显高于其他年龄组,差异有统计学意义(P<0.05);小于1个月组患儿CT检出率要高于其他组,差异有统计学意义(P<0.05)。结论夏季易检出MP和CT;3岁以上患儿较其他年龄段患儿易检出MP;新生儿较其他年龄段患儿易检出CT。荧光定量聚合酶链反应法诊断支原体、衣原体感染快速、敏感,特异性高。     

IPAzyme Chlamydia and microimmunofluorescence tests for detecting antibodies against Chlamydia trachomatis in women undergoing laparoscopy

Superficial Chlamydia trachomatis infections are diagnosed by antigen detection whereas serological investigations are mainly performed to detect deep-seated infections. In this study the genus-specific IPAzyme and two species-specific microimmunofluorescence (MIF) tests were compared for detecting antibodies against C. trachomatis in women undergoing laparoscopy for diagnostic purposes or for ligation of the fallopian tubes. Microbiological findings were similar in both groups. C. trachomatis was detected in 4 of 38 women with ligation and in 6 of 61 women with diagnostic laparoscopy. Serum IgG antibody titres by IPAzyme were clearly positive in 24 out of 61 of the diagnostic group and in 12 out of 38 of the ligation group. However, 14 (39%) of the 36 IPAzyme-positive results were caused by antibodies against Chlamydia pneumoniae and/or Chlamydia psittaci, only 4 (11%) were caused by anti-C. trachomatis IgG and 8 (22%) were caused by both antibodies as tested by a conventional MIF; in 10 all MIF titres were lower than 1:32. A commercial MIF still containing too much genus-specific lipopolysaccharides was more sensitive, but also more crossreactive than the conventional MIF. IPAzyme IgA were only found in association with positive IPAzyme IgG results. We conclude that in case of presumed deep-seated C. trachomatis infection only sensitive species-specific assays for detecting C. trachomatis antibodies are helpful. IPAzyme and other genus-specific assays cannot be recommended to detect antibodies to C. trachomatis in the serum, because positive results are caused mainly by antibodies against respiratory chlamydiae.     

不孕与支原体、衣原体感染和抗精子抗体的关系

目的探讨女性支原体、衣原体感染和抗精子抗体阳性与不孕的关系,以指导临床治疗及提高不孕患者的治愈率。方法选择于我院2011年2月~2012年2月就诊的不孕女性患者365例（观察组）进行支原体（UU、MH）、衣原体（CT）与血清抗精子抗体（ASA）检测,同时选择66例正常妇女（对照组）作对照。结果不孕症观察组支原体、衣原体、抗精子抗体阳性检出率均明显高于对照组（P〈0.05）。不孕组中血清抗精子抗体阳性者支原体、衣原体感染较阴性者高,其差异有统计学意义（P〈0.05）。结论生殖道支原体、衣原体感染及血清抗精子抗体阳性是导致不孕的重要原因,抗精子抗体的产生与支原体、衣原体感染有关。     

沙眼衣原体感染不同检测方法的比较

目的：研究不同方法检测沙眼衣原体感染的灵敏度和特异度。方法采取213例女性非淋菌性泌尿生殖道感染者宫颈分泌物,分别用直接免疫荧光法（DFA）、免疫层析法（ICA）和实时荧光定量PCR法（FQ-PCR）检测沙眼衣原体。结果采用DAF法和FQ-PCR法与采用ICA法检测沙眼衣原体阳性率比较差异有统计学意义（P＜0．05）;DFA法、ICA法和FQ-PCR法的灵敏度分别为95．1％、60．2％和97．3％,特异度分别为93．2％、99．2％和99．3％。DFA法和FQ-PCR法的灵敏度高于ICA法,差异有统计学意义（P＜0．05）,ICA法和FQ-PCR法的特异度高于DFA法,差异有统计学意义（P＜0．05）。结论 FQ-PCR法有较高的灵敏度和特异度,可为临床诊断沙眼衣原体感染提供可靠依据。基层医疗卫生单位适合用IC A法检测沙眼衣原体。     

Moving to nucleic acid-based detection of genital Chlamydia trachomatis

Laboratory diagnosis of the bacterium Chlamydia trachomatis has gone through a complete phase of evolution since it was first identified as a significant cause of sexually transmitted infection. As a fragile, obligatory intracellular organism, it was initially only grown in eggs. Subsequently, diagnosis relied on culture in continuous cell lines. To address the limitations of culture, immunological methods were developed and direct antigen detection using enzyme immunoassay and immunofluorescence flourished. With the advent of molecular technologies, nucleic acid-based amplification techniques became the methods of choice, offering improved standard of care for diagnosis and opening up the possibility of screening using noninvasive, patient-acceptable specimens. In this article, the various currently available molecular methods are examined, some of the existing problems discussed and a view on what we think might happen in the next 5 years to the technology and requirement in diagnosis and screening is given.     

Enzyme-linked immunosorbent assay for the detection of antibody to Chlamydia trachomatis and Chlamydia psittaci

An enzyme-linked immunosorbent assay (ELISA) was developed with use of a strain of Chlamydia trachomatis, lymphogranuloma venereum serotype 2. The ELISA reactions were monitored by absorbance at 492 nm in a spectrophotometer. A positive control method based on one serum dilution was used to assign ELISA titers to the sera. Sera that had been tested with use of complement fixation (CF) and microimmunofluorescence (MIF) tests were examined with ELISA. ELISA was more sensitive than the CF test and as sensitive as the MIF test. Serum pairs that had diagnostic titer rises in the other tests also had such rises in ELISA. ELISA is a simple, sensitive assay for detection of antibody to C. trachomatis.