生漆多糖对免疫功能的影响

生漆多糖对免疫功能的影响武汉市劳动卫生职业病防治研究所（武汉４３００７１）富天玲，陈露华，刘善良，汤森元武汉大学资源开发与应用技术研究所杜予民，孔振武生漆多糖是一种水溶性酸性植物多糖，对机体的生物活性，国内外未见报道。根据某些植物（如人参等）多糖对机...     

向日葵茎芯多糖对小鼠免疫功能的增强作用

菊科植物向日葵茎芯煎剂对小鼠移植瘤有显著抑制作用。付定一等报道,从向日葵茎芯提取的向日葵茎芯多糖(Helianthusannuus Polysaccharides,     

大理百合多糖对小鼠免疫功能的影响

目的：观察大理百合多糖（Lilium polysaccharide,LP）小鼠免疫功能的影响。方法：昆明种小白鼠每天分别腹腔注射给药（ip）LP100mg·kg-1．BW,150mg·kg-1．BW,300mg·kg-1·BW,对照组则给等量生理盐水（NS）,连续14天后,观察LP对小鼠免疫功能的影响。结果：LP能显著增加小鼠免疫器官重量,促进小鼠单核巨噬细胞的吞噬功能,提高小鼠血清溶血素的生成,能明显促进ConA诱导的淋巴细胞增殖转化,能显著提高小鼠NK细胞活性。结论：LP对小鼠免疫功能能有显著促进作用。     

四物汤对小鼠免疫功能的影响

四物汤源于《和剂局方》（组成：当归、熟地、川芎和白芍），为中医补血的基本方；也是中医妇科病和内科病常用加减方。虽该方中的单味药，如当归、熟地和白芍有提高免疫功能和抗炎的作用［１］，但四物汤对免疫功能的影响如何，未见报道。本研究表明，四物汤可提高Ｂａｌ...     

三氧化二砷对H22肝癌小鼠免疫功能的影响

三氧化二砷治疗急性粒细胞性白血病在临床上已取得了一定的功效 ,大量研究表明ＡＳ2 Ｏ3 对肿瘤细胞具有促进凋亡作用。目前有学者推测 ,ＡＳ2 Ｏ3 可能是通过增强机体免疫功能达到抗肿瘤效应。我们通过研究ＡＳ2 Ｏ3 对Ｈ2 2 肝癌小鼠外周血的Ｔ细胞亚群及ＮＫ细胞活性的影响来探讨ＡＳ2 Ｏ3 的抗肿瘤作用。我们把Ｈ2 2 腹水型荷肝癌小鼠的腹水瘤细胞接种于小鼠前肢外侧皮下 ,制成实体型荷瘤鼠模型。将小鼠 40只随机分为正常组、荷瘤对照组 (生理盐水0 2ｍｌ ｄ)、低剂量治疗组 (ＡＳ2 Ｏ3 2ｍｇ ｋｇ)、作者单位 :15 0 0 0 1哈尔滨医科大…     

硒酸软骨多糖对Hca-F荷瘤小鼠免疫功能的影响

植物多糖具有许多有益的治疗效果,如抗肿瘤、免疫调控、伤口愈合等.其机制被认为是通过调控天然免疫,特别是巨噬细胞的功能而达到的[1,2].但是对于动物来源的多糖,其研究相对较少.本实验室从猪软骨里提取了多糖,并进行了一系列的研究.     

益生菌FGM 发酵黄芪多糖对脑损伤小鼠免疫功能的影响

目的:探讨益生菌FGM 发酵黄芪多糖对脑损伤模型小鼠免疫功能的影响。方法:益生菌FGM 菌株发酵黄芪药材,所得发酵黄芪粉采用水提醇沉法提取发酵黄芪多糖,发酵黄芪多糖高、中、低浓度给药组小鼠在造模前7 天每天分别腹腔注射剂量为200、100、50 mg/ kg 的发酵黄芪多糖,于末次给药1 h 后采用线栓法建立小鼠脑损伤模型。称重法测定小鼠脾脏指数、胸腺指数,采用MMT 法评价脾淋巴细胞增殖情况。采用酶联免疫吸附法(ELISA)检验炎性因子肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)的表达水平。结果:与模型组比较,发酵黄芪多糖高浓度给药组中脾脏指数、胸腺指数显著升高(P<0.05);脾淋巴细胞增殖能力显著升高(P<0.05);TNF-α和IL-1β的含量显著下降(P<0.05)。结论:益生菌FGM 发酵黄芪多糖对脑损伤小鼠具有免疫保护作用。     

保元丹对小鼠免疫功能的实验研究

目的 了解保元丹对机体免疫功能的影响。方法 采用Balb/c健康雌性小鼠为实验动物模型,检测了保元丹对淋巴细胞转化值,半数溶血值,抗体生成细胞数,迟发性变态反应以及NK细胞活性的影响。结果 与对照组相比,保元丹高剂量组能显著提高小鼠血清溶血素半数溶血值(P<0.05)。中高剂量能显著提高小鼠淋巴细胞转化值和迟发性变态反应(P<0.05)。低中高3个剂量都能显著提高抗体生成细胞数和NK细胞活性(P<0.01)。结论 保元丹既能增强特异性免疫又能增强非特异性免疫,表明保元丹具有提高机体免疫功能的作用。     

玉屏风散多糖对小鼠免疫功能的影响

目的分析玉屏风散多糖类成分对小鼠免疫功能的影响。方法用环磷酰胺制作免疫功能下降的小鼠模型;利用腹腔巨噬细胞吞噬实验观察药物对机体非特异性免疫产生的影响;采用溶血素抗体方法分析药物对体液免疫应答的影响;利用DNCB迟发型超敏实验,分析药物将对细胞免疫的影响;同时用ELISA法检测小鼠脾细胞IL-2的分泌量、肠道灌流液和呼吸道里面的IgA含量。结果玉屏风散总提取物和多糖类成分对上述各项指标均有明显增强效果;防风对于促进呼吸道和道肠道的IgA分泌有明显效果,而对细胞免疫的影响不大;白术只加强了巨噬细胞的吞噬能力。结论玉屏风散中的多糖类成分是发挥免疫干预作用的主要成分。     

龙牙楤木多糖抗肿瘤活性及对荷瘤小鼠免疫功能的影响

目的:探讨龙牙楤木多糖(AEPS)抗肿瘤活性及对荷瘤小鼠免疫功能的影响。方法:以S180肉瘤为肿瘤模型,检测龙牙楤木多糖对肿瘤生长的抑制活性;MTT法检测龙牙楤木多糖对S180肉瘤细胞、A549肺癌细胞、SMMC-7721肝癌细胞的体外抑制活性;以其对荷瘤小鼠免疫器官、血液淋巴细胞数量及淋巴细胞增殖影响、巨噬细胞活性和NK细胞杀伤活性来评价AEPS对荷瘤小鼠免疫功能的作用。结果:AEPS对S180肉瘤生长有显著的抑制作用,其中75 mg/(kg.d)剂量组抑瘤率最高达57.68%;AEPS对S180肉瘤细胞、A549肺癌细胞、SMMC-7721肝癌细胞生长的最高抑制率均达60%以上;AEPS显著提高荷瘤小鼠脾脏和胸腺质量以及血液淋巴细胞数量,促进淋巴细胞增殖反应,增加NK细胞杀伤活性和巨噬细胞活性。结论:AEPS有显著的抗肿瘤活性,并能直接作用于肿瘤细胞,抑制肿瘤生长,其抑瘤作用与机体免疫功能的增强有关。     

Protective effect of Plantago major L. Pectin polysaccharide against systemic Streptococcus pneumoniae infection in mice

The antibacterial effect of a soluble pectin polysaccharide, PMII, isolated from the leaves of Plantago major, was examined in inbred NIH/OlaHsd and Fox Chase SCID mice experimentally infected with Streptococcus pneumoniae serotype 6B. Serotype 6B is known to give a more protracted infection when injected intraperitoneally into susceptible mice than more virulent serotypes like type 4. PMII was administered i.p. either once 3 days before challenge or once to thrice from 3 to 48 h after challenge. The number of bacteria in blood and the mouse survival rate were recorded. Pre-challenge administration of PMII and also lipopolysaccharide (LPS), included as a control, gave a dose-dependent protective effect against S. pneumoniae type 6B infection. However, injection of PMII after establishment of the infection in NIH/OlaHsd mice had no effect. The data demonstrate that, firstly, the polysaccharide fraction PMII from P. major protects against pneumococcal infection in mice when administered systemically prechallenge, and secondly that the protective effect is owing to stimulation of the innate and not the adaptive immune system.     

白扁豆多糖对免疫抑制小鼠的免疫调节作用

目的探讨白扁豆多糖(polysaccharide from Dolichos lablab L.,DLP)对环磷酰胺(cyclophosphamide,CTX)所致的免疫抑制小鼠的免疫调节作用。方法小鼠随机分为正常对照组、模型组、香菇多糖(Lentinan,LNT,15 mg/kg)和白扁豆多糖低、中、高剂量组(75、150、300 mg/kg)。通过对小鼠连续3 d腹腔注射环磷酰胺(100 mg/kg)建立小鼠免疫抑制模型,造模后连续灌胃给药7 d,检测免疫器官重量指数、血清溶血素、脾淋巴细胞增殖、脾脏NK细胞活性及小鼠血清细胞因子IL-2、IL-4和INF-γ水平,观察白扁豆多糖的免疫调节作用。结果与正常对照组相比,模型组小鼠的免疫器官重量指数、血清溶血素、脾淋巴细胞增殖、脾脏NK细胞活性及小鼠血清细胞因子IL-2、IL-4和INF-γ水平均明显降低(P<0.05,P<0.01),而中、高剂量DLP和香菇多糖可明显减轻由环磷酰胺引起的上述各项指标的下降(P<0.05,P<0.01)。结论 DLP能改善环磷酰胺所致免疫抑制小鼠的免疫功能。     

Effect on the immunological competence of Physalis pubescens L. in mice

Effect of different parts of the Physalis pubescens L. (Pp) on the immunocompetence were investigated in Kunming mice inbred lines. After 35 consecutive days of feeding, the mice were sacrificed to evaluate the immunologic function through the relative weights of spleen and thymus, the delayed type hypersensitivity (DTH), carbon particle clearance test and serum agglutination test. The results are summarised as follows: the pulp of Pp had statistically significant effect on the mice's non-specific immunity, but not on the weight of the mice's thymus. The Pp's pulp, peel and seeds had significant effect on the mice's cellular immunity, respectively, they had regulating effect on the mice's T-lymphocytes. Any part of Pp didn't have statistically significant effect on the mice's humoral immunity.     

番茄红素对实验小鼠免疫功能的影响

目的:应用中药血清药理学方法,观察番茄红素对实验小鼠免疫功能的影响。方法:用6周龄,体重为18~20g的BALB/c小鼠200只,设0、10、15和20 mg/kg.bw四个番茄红素水平处理。于给药后0.5、1、1.5、2、2.5、3、3.5、4、4.5和5小时分别无菌条件下取血,分离血清进行脾脏T细胞增殖活性和分泌IL-2、IL-4活性、NK细胞杀伤活性等免疫功能检测。结果:不同剂量番茄红素对脾淋巴细胞增殖、IL-2、IL-4产生有促进作用,且不同时相有所波动;能明显增强NK细胞活性,以高剂量组最为明显,作用时间较长;其药效影响集中在给药后0.5~3小时。结论:适宜剂量的番茄红素能增强特异性与非特异性免疫,表明有提高机体免疫功能的作用。     

亮菌多糖对S180荷瘤小鼠免疫功能的研究

目的研究亮菌多糖对荷瘤小鼠免疫功能的影响。方法用S180瘤细胞建立动物模型,通过腹腔注射观察亮菌多糖对荷瘤小鼠淋巴细胞增殖反应、NK细胞活性及IL-2诱生的影响。结果亮菌多糖能够显著提高荷瘤小鼠淋巴细胞的增殖能力、NK细胞活性、显著促进脾细胞产生IL-2。结论亮菌多糖能够显著提高机体免疫调节功能。     

Interaction between human complement and a pectin type polysaccharide fraction, PMII, from the leaves of Plantago major L

The interaction between a pectin type polysaccharide fraction, PMII, isolated from the leaves of Plantago major, and human complement was tested in two different hemolytic complement-fixation tests and in addition by two ELISA methods detecting complement-activation products. Sera were used as a complement source of 10 arbitrary human volunteers, individually and as a pool. The complement-fixation tests were designed to measure the concentration of the pectin necessary to inhibit 50% of the hemolysis (ICH(50)). The ELISA tests for complement-activation products were measured in AU/mg using a fully activated serum as a standard. We observed a more than 200-fold difference in ICH(50) activity of the PMII pectin in one of the hemolytic tests by varying the individual sera used as complement-source. On the other hand, the ELISA complement-activation tests showed no significant variation in activity of the PMII depending on the complement-serum used. The level of antibodies against PMII detected in the complement-sera did not correlate with the ICH(50) activity of PMII. The results show that PMII is a potent complement activator with an activity of the same order of magnitude on a weight basis as that of aggregated human immunoglobulin (Ig)G. This activation leads to a complement consumption probably explaining the PMII's effect in the complement-fixation tests. PMII seems to be an activator both on the classical and the alternative pathway of activation. The results might be related to the reported wound-healing effect of the leaves of Plantago major.     

Effects of taxol on the macrophage function. Interactions with some immunological parameters.

The effects of Taxol on some immunological parameters were investigated, in vitro, in the murine macrophage cell line J774.1. Mitochondrial dehydrogenase activity and (3H) TdR incorporation were shown to be increased in Taxol treated cells. Likewise, Taxol induced TNF alpha secretion. But Taxol seemed to be a weak inducer of the two interleukins IL-1 and IL-2, since their detection occurred late in the cell culture supernatants.     

乌索酸对小鼠免疫功能影响的实验研究

乌索酸((Ursolic acid又名熊果酸、乌苏酸)是从忍冬科接骨木属植物陆英(Sambucus Chinensis Lindl)中提取得到的单一化合物[1].研究表明, 乌索酸对BCG LPS免疫性肝损伤有明显的保护作用[2],本实验为进一步研究乌索酸的保肝作用机理,建立不同的小鼠免疫模型,从多角度研究乌索酸对小鼠免疫功能的影响.     

飞龙掌血水提物对心肌缺血兔心功能和血液动力学的影响

目的:观察飞龙掌血(Toddalia asiatica Lam)水提物(飞龙一号,F01)对急性心肌缺血动物模型心脏功能和血液动力学的影响。方法:新西兰兔作不开胸膜冠脉左前降支高位结扎造成急性心肌缺血, 观测结扎后及F01 ip对动物心功、血液动力学的影响。结果:冠脉左前降支结扎后,LVEDP显著升高(P＜0.05),t-dp/dt max 无明显变化,其余各项参数均明显降低(P＜0.05或P＜0.01)。F01(268 mg/kg)腹腔注射后,除有关左室作功和心肌耗氧的参数(HR、TTI和TTI×HR)继续降低外,其余各项参数的变化均显著逆转,恢复或接近结扎前的水平, 用药后1.5 h的作用较0.5 h更明显。结论:F01可显著减少急性缺血心肌的作功和耗氧,通过纠正心脏对氧的供需平衡失调,改善心脏收缩、舒张功能和泵血功能,从而发挥对缺血心肌的保护作用。