保留残端或剩余束支并骨膜包绕肌腱重建前交叉韧带的临床研究

目的进行关节镜下保留残端或剩余束支并骨膜包绕自体肌腱重建前交叉韧带(ACL)的临床研究,探寻ACL重建术后移植肌腱与骨隧道愈合的有效方法。方法随机选取2010年7月²013年6月共89例有韧带残端存留的ACL损伤患者入选本研究组,将患者随机分为实验组和对照组,实验组46例,对照组43例。分别进行关节镜下前交叉韧带重建手术,实验组予以保留前交叉韧带残端及剩余束支并骨膜包绕,对照组予以清理残端无骨膜包绕。术后随访122013年6月共89例有韧带残端存留的ACL损伤患者入选本研究组,将患者随机分为实验组和对照组,实验组46例,对照组43例。分别进行关节镜下前交叉韧带重建手术,实验组予以保留前交叉韧带残端及剩余束支并骨膜包绕,对照组予以清理残端无骨膜包绕。术后随访12²9个月,对两组患者的轴移试验、Lachman试验、IKDC分级、KT-1000评分、Lysholm评分结果进行比较,并进行二次关节镜探查移植物滑膜覆盖情况。结果实验组轴移试验、Lachman试验和IKDC分级的阴性率均高于对照组,实验组的KT-1000评分和Lysholm评分均高于对照组,差异有统计学意义(P<0.05或P<0.01)。两组患者滑膜覆盖分型构成差异有统计学意义(P<0.01)。实验组滑膜覆盖良好率高于对照组,差异有统计学意义(P<0.05)。结论保留交叉韧带残端重建ACL,有助于移植肌腱再血管化及爬行替代,有利于维持膝关节稳定性。     

关节镜微创技术在ACL重建中的应用及临床分析

目的探讨及分析关节镜在膝关节前交叉韧带断裂韧带重建中的应用。方法前交叉韧带断裂36例患者行关节镜下膝关节人工韧带重建术,结果前交叉韧带断裂术均顺利进行,术后早期进行功能锻炼,处理相关并发症,并对其术前、术后6个月、术后12个月的稳定性进行分析。结果术后36例患者随访时间6～24个月,术前前抽屉试验结果为阳性,Lachman试验结果为阳性。术后均阴性。lysholm评分术前31～72分(54±3.63),术后6个月为(72.9±7.7)分,术后12个月为(86.8±8.3)分,术前与术后比较差异有统计学意义(P<0.01)。结论关节镜下重建前交叉韧带是一种创伤小,用时短,疗效确切的治疗方式,人工韧带重建ACL早期效果满意;手术中应尽量保留较长的韧带残端和使用准确的等长重建方法。     

关节镜下保留与清除断裂前交叉韧带残端重建术后疗效比较

目的:对比关节镜下保留与清除断裂前交叉韧带（ACL）残端重建术后临床疗效。方法:回顾性分析70例获得12个月以上随访的ACL断裂患者临床资料,其中对照组35例采用传统清除残端重建ACL,观察组35例采用保留残端重建ACL,比较2组患者术后关节稳定性、关节功能及本体感觉恢复情况。结果:所有患者均获得随访,随访时间为12～27个月。2组患者术后关节稳定性差异无统计学意义（P>0.05）;末次随访时观察组患者术后关节功能均优于对照组（P<0.01）;观察组患者术后本体感觉优于对照组（P<0.01）。结论:关节镜下保留与清除残端重建ACL均能取得较好的临床疗效,恢复关节稳定性;但保留残端手术更有利于术后膝关节功能及本体感觉的恢复。     

关节镜下同时保留股骨及胫骨残端重建前交叉韧带的临床疗效评估

目的 探讨关节镜下采取同时保留股骨及胫骨残端方式重建前交叉韧带的技术应用并观察其效果.方法 选取安徽医科大学第一附属医院2014年1月至2016年1月收住的前交叉韧带(ACL)损伤患者52例,术前依据随机数字表法分为同时保留胫骨及股骨韧带残端重建ACL组(保残组)与不保留任何韧带残端重建ACL组(对照组)并按照单束重建ACL方法完成手术,每组26例.采用Lysholm膝关节功能评分评估客观功能及行为能力,采用国际膝关节文献委员会(IKDC)主观评分评估主观运动能力及生活质量,比较两组患者术前、术后18个月上述评分标准评估的患者膝关节功能.所有患者于术后6个月复查磁共振,观察移植肌腱与骨隧道的愈合情况及有无其他关节异常信号.结果 保残组患者膝关节术前Lysholm评分和IKDC评分均低于术后[(57.21±5.05)分vs(89.43±4.12)分;(52.18±7.61)分vs(91.43±4.52)分],差异有统计学意义(P<0.05).对照组患者术前Lysholm评分和IKDC评分均低于术后[(54.72±4.06)分vs(86.21±3.89)分;(56.24±6.59)分vs(83.56±5.02)分],差异有统计学意义(P<0.05).术后IKDC主观评分保残组优于对照组[(91.43±4.52)分vs(83.56±5.02)分],且差异有统计学意义(P<0.05).所有患者术后未发生严重并发症.结论 关节镜下同时保留胫骨及股骨侧残端重建前交叉韧带术术后效果良好,无不良并发症,有利于前交叉韧带重建术后膝关节功能的恢复.     

关节镜下保留胫骨残端重建前交叉韧带50例报告

目的:探讨保留残端重建前交叉韧带(anterior cruciate ligament ACL)的临床疗效.方法:应用股薄肌、半腱肌肌腱治疗ACL损伤50例,术中保留ACL下止点残端.结果:术后膝关节稳定性增强,Lysholm功能评分,术前平均69.5分,术后平均91.6分,提高22.1分.Lachman试验阴性47例...     

关节镜下前交叉韧带保留残端重建术与不保留残端重建术治疗前交叉韧带损伤患者的效果比较

目的:比较关节镜下前交叉韧带(ACL)保留残端重建术与不保留残端重建术治疗ACL损伤患者的效果。方法:选取60例ACL损伤患者为研究对象,按照随机数字表法分为观察组和对照组各30例。观察组采用关节镜下保留残端重建术治疗,对照组采用关节镜下不保留残端重建术治疗,术后6个月,比较两组Lysholm膝关节功能评分、患侧膝关节被动活动觉察阈值、IKDC韧带分级、患侧膝关节位置感觉测定值(JPS)和并发症发生率。结果:术后6个月,两组Lysholm评分均高于术前,且观察组高于对照组,两组患侧膝关节被动活动觉察阈值均低于术前,且观察组低于对照组,差异有统计学意义(P<0.05);观察组IKDC韧带分级优于对照组,差异有统计学意义(P<0.05);两组患侧膝关节JPS均低于术前,且观察组低于对照组,差异有统计学意义(P<0.05);观察组并发症发生率为16.67%,与对照组的23.33%比较,差异无统计学意义(P>0.05)。结论:关节镜下ACL保留残端重建术治疗ACL损伤患者可提高Lysholm评分,改善IKDC韧带分级,以及降低膝关节被动活动觉察阈值和患侧膝关节JPS,优于不保留残端重建术治疗效果。     

关节镜下清除和保留断裂前交叉韧带残端重建术后疗效对比

目的探讨前交叉韧带(anterior cruciate ligament,ACL)断裂予施行膝关节镜下ACL单隧道单束重建术,分析清除和保留断裂ACL残端术后的疗效是否存在差异,探索出更适合临床操作的手术方式。方法 2008年1月—2013年6月,对40例ACL断裂施行膝关节镜下单隧道单束ACL重建术,移植物均为取自同侧半腱肌和股薄肌编织成的自体韧带,其中20例(A组)为断裂ACL残端进行清除干净后再施行膝关节镜下单隧道单束ACL重建术,20例(B组)为断裂ACL残端进行尽可能保留前提下施行膝关节镜下单隧道单束ACL重建术,手术均为同一组医师完成,2组手术前后Lysholm评分和IKDC评分结果进行t检验。结果 40例均获随访,时间为12~28个月,平均(20.7±3.7)个月,手术前后Lysholm评分和IKDC评分t检验结果 ,所得P=0.0183和P=0.0177,P值均<0.05,比较差异有统计学意义。结论 ACL断裂采用清除和保留断裂前交叉韧带残端进行自体韧带重建术后的疗效,在关节平衡性、关节自体感觉恢复存在显著性差异。保留ACL残端进行单隧道单束重建ACL,有利于移植肌腱的胫骨和股骨端隧道口的制作;ACL固定于原ACL残端中,避免重建的ACL在髁间窝被撞击;原ACL残端纤维围绕重建的ACL,可以覆盖隧道内口,减少关节液对移植肌腱与隧道愈合的影响。     

自体腘绳肌腱单束重建前交叉韧带临床研究

目的:探讨关节镜下采用自体肌腱,解剖重建修复前交叉韧带损伤的临床效果。方法:收集前交叉韧带(ACL)损伤患者43例,对其进行关节镜下保留残端的自体肌腱单束解剖重建术,术后进行随访。对患者的膝关节功能和稳定性进行评估。结果:所有病例均获得随访,随访时间6~15月,平均9.7月。膝关节Lysholm评分,由术前平均(42.45±5.46)分增加至术后1年(88.65±6.82)分;Tegner评分由术前平均3.8分增加至术后平均5.5分,前后比较差异均具有统计学意义。结论:采用膝关节镜下腘绳肌腱重建修复前交叉韧带损伤,膝关节功能评分术后较术前明显改善,无特殊并发症。可见关节镜技术具有很好的临床效果和安全性。     

关节镜下保留残端重建前交叉韧带术对股四头肌萎缩的影响

目的:观察关节镜下保留残端重建前交叉韧带术对股四头肌萎缩及膝关节稳定性的影响.方法:前交叉韧带损伤患者51例,随机分为保留残端组27例和清除残端组24例.两组均在关节镜下行自体腘绳肌腱单束重建前交叉韧带术,保留残端组清理前交叉韧带残端,保留韧带长度约1/3,清除残端组清除韧带残端.比较两组患者股四头肌周径萎缩指数及Ly...     

膝关节镜下前、后交叉韧带同期重建的疗效观察

目的探讨采用关节镜下微创手术的方法同期重建膝关节前交叉韧带（ACL）与后交叉韧带（PCL）。方法15例病人（15膝）经关节镜检查证实ACL和PCL均断裂,在膝关节镜下采用挤压螺钉固定同种异体跟腱、LARS人工韧带或自体双侧4股胭绳肌腱移植同期重建前、后前叉韧带。术后随访6—18个月,平均随访14个月。结果按Lysholm膝关节评分标准评分,平均积分由术前的24分提高到术后的85分,全组均未出现并发损伤。患者均在术后3—6个月恢复正常的工作及生活。结论关节镜下同时重建前、后交叉韧带手术创伤小,骨隧道定位准确,固定牢固,术后膝关节功能恢复满意。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.