Cataract Surgery for Tilted Lens in Peters’ Anomaly Type 2

Background

Cases of cataract surgery without penetrating keratoplasty in patients with Peters’ anomaly are very rare. We report a case of Peters’ anomaly type 2 with tilted lens due to synechia between the lens and iris that was treated with cataract surgery without penetrating keratoplasty.

Case Presentation

A 16-year-old girl had Peters’ anomaly in both eyes. Corneal opacity was severe in the left eye due to high-grade dysgenesis of the anterior segment. In the right eye, corneal opacity had spread from the center of the cornea to the inferotemporal side, and there was synechia between the iris and corneal endothelium from the inferonasal side to the inferotemporal side. Opacity was observed in the anterior pole of the lens, and there was synechia between the anterior iris and the lens. Ultrasound biomicroscopy (UBM) revealed that the lens was tilted because of synechia. The tilted lens induced astigmatism, which reduced visual acuity to 20/250, in conjunction with a cataract. Cataract surgery was performed; the synechia between the lens capsule and the iris was severed, an intraocular lens was inserted, and the tilt was repaired. UBM was used postoperatively to confirm that the lens capsule synechia had been corrected and that the intraocular lens was not tilted. As a result, visual acuity improved to 20/100; glaucoma and expansion of corneal opacity were not observed.

Conclusions

Severing of the synechia between the cataract and iris, during cataract surgery, in a patient with Peters’ anomaly type 2 resulted in favorable postoperative visual acuity.Key words: Peters’ anomaly type 2, Synechia, Phacoemulsification, Tilted lens     

The prevalence of cataract in a population with and without type 2 diabetes mellitus

Purpose: To evaluate the prevalence and risk factors of lens opacities in a geographically defined population of subjects with type 2 diabetes mellitus compared with a control population. Methods: Subjects in the community of Laxå with a diagnosis of type 2 diabetes mellitus (n = 275) and a control group (n = 256) participated in the study. Lens opacities were graded with Lens Opacities Classification System II in all participants. Lens Opacities Classification System score ≥2 was considered as significant lens opacity. Anthropometric and blood chemistry data were collected for all participants in connection with the eye examination. For the diabetic population, yearly updated information on glucose control, blood pressure and body mass index was available through medical records from diabetes diagnosis until the time of the eye examination. Results: The prevalence of significant cortical, posterior subcapsular and nuclear cataract was 65.5%, 42.5% and 48.0%, respectively, in the type 2 diabetes population in Laxå. In logistic regression analyses, all types of lens opacities were strongly associated with age (p < 0.0001). Cortical lens opacity was also associated with a diagnosis of diabetes (p < 0.0001), posterior subcapsular lens opacity with HbA1c (p < 0.0001) and nuclear lens opacity with female gender and higher heart rate (both p = 0.0004). In the diabetic population, all types of cataract were likewise strongly associated with age (p < 0.0001), posterior subcapsular cataract with HbA1c (p = 0.0032), nuclear cataract with female gender (p = 0.0002) and higher heart rate (p = 0.0008). Conclusions: Our study shows that cortical cataract is associated with diabetes mellitus, not necessarily defined by glucose control, whereas posterior subcapsular cataract is associated with glucose levels. Nuclear cataract is not associated with diabetes mellitus, but is more frequent in women and is also associated with higher heart rate.     

葡萄膜炎并发白内障的手术治疗

目的:探讨葡萄膜炎并发白内障施行超声乳化吸除人工晶状体植入治疗的临床效果。方法:对19例21眼稳定的葡萄膜炎并发白内障患者行超声乳化吸除晶状体植入术,术中根据虹膜粘连的程度和范围,分别采用不同的方法处理粘连虹膜及小瞳孔。结果:患者19例21眼术后19眼视力改善,总有效率90%,其中术后视力≥0.3者15眼(71%)。术后无严重并发症发生,无葡萄膜炎加重。结论:超声乳化吸除人工晶状体植入术是治疗葡萄膜炎并发白内障的有效方法。术前控制炎症稳定3mo,术中掌握处理虹膜粘连及小瞳孔的要点,慎重操作,可减少术后并发症,改善患者视功能。     

Crygf(Rop): the first mutation in the Crygf gene causing a unique radial lens opacity

PURPOSE: The Rop (radial opacity) mutation, which was recovered in a mutagenicity screen after paternal treatment with procarbazine, was analyzed to determine phenotype, chromosomal localization, candidate genes, and molecular lesion. METHODS: Native lenses were photographed under a dissecting microscope. Histologic sections of the eye were made according to standard procedures. Fine mapping of the mutation in relation to microsatellite markers for mouse chromosome 1 was performed. Candidate genes were amplified by PCR from cDNA or genomic DNA and sequenced. RESULTS: The nuclear opacity of the heterozygous mutants showed radial structures, whereas the opacity of the homozygotes was homogenous. The histologic analysis revealed changes in the lens nucleus, which corresponds to the pronounced opacification in lenses of homozygous mutants. The allelism of Rop to the Cat2 group of dominant cataracts on mouse chromosome 1 was confirmed by linkage to microsatellite markers D1Mit156 and D1Mit181. The cluster of the Cryg genes and the closely linked Cryba2 gene were tested as candidates. A T-->A exchange in exon 2 of the Crygf gene leads to a Val-->Glu exchange in codon 38 and was considered to be causative for the cataract phenotype; therefore, Crygf(Rop) has been suggested as the designation for the mutation. CONCLUSIONS: Crygf(Rop) is the first mutation affecting the Crygf gene. Dominant cataract mutations for all six Cryg genes on mouse chromosome 1 have now been characterized, demonstrating the importance of this gene cluster in lens transparency.     

兔眼玻璃体切割术后填充物对并发性白内障的影响

目的:探讨兔眼玻璃体切割术后晶状体混浊的情况变化。方法:选用Dutch Bbelted兔63只,随机分为3组,玻璃体切割联合平衡盐填充(平衡盐组);玻璃体切割联合200mL/LC3F8填充(气体组);玻璃体切割联合硅油填充(硅油组)。观察3mo晶状体混浊变化情况及术后不同时间眼压变化情况。结果:玻璃体切割术后玻璃体腔内填充200mL/LC3F8气体,术后并发性白内障的发生率为85.7%;填充硅油术后并发性白内障的发生率为19.1%;填充平衡盐术后并发性白内障的发生率为14.3%,各组术后眼压较术前变化稳定,无统计学意义。结论:兔眼玻璃体切割术后气体注入组晶状体混浊的发生率最高,术后尽早取出硅油可以降低术后晶状体混浊的发生率。     

硫醇转移酶基因敲除小鼠模型的建立及其白内障形成机制

目的:通过建立硫醇转移酶(TTase)基因敲除小鼠模型,观察其晶状体形态和生化方面随年龄的改变,探讨TTase在晶状体氧化还原系统中的重要作用及参与年龄相关性白内障形成的机制。方法:建立TTase基因敲除小鼠模型并进行基因型鉴定。裂隙灯观察TTase基因敲除型和野生型小鼠白内障随年龄的发生情况。检测小鼠晶状体中谷胱甘肽(GSH)含量。Western blot检测晶状体中氧化产物蛋白质二硫化物(PSSG)的变化。免疫共沉淀法鉴定形成PSSG的蛋白质。观察纯化的重组人晶状体TTase(RHLT)对PSSG的脱硫醇作用。结果:TTase基因敲除型小鼠和野生型小鼠白内障的发生均随年龄而增加,且主要表现为核性白内障。在TTase基因敲除型小鼠中,白内障最早从4月龄开始发生,9月龄时最为显著;野生型小鼠白内障最早从9月龄开始发生,12月龄时最为显著。两种基因型小鼠晶状体中GSH含量均随年龄增加而下降,9月龄TTase基因敲除型小鼠晶状体中GSH下降更为显著,且PSSG的表达明显高于野生型,主要表现为高分子聚合物。免疫共沉淀反应证实形成PSSG的蛋白质包含肌动蛋白(actin)和三磷酸甘油醛脱氢酶(GAPDH),这种积聚的PSSG可被GSH还原,且与纯化的RHLT反应更有效。结论:TTase基因敲除可以加速小鼠年龄相关性白内障的发生,这与晶状体中PSSG的积聚相关,且形成的PSSG可被TTase脱硫醇,证实TTase在防止年龄相关性白内障的发生中发挥重要作用。     

严重粘连小瞳孔白内障超声乳化及折叠人工晶状体植入术

目的:探讨严重粘连小瞳孔白内障术中采用瞳孔缘连续环形剪除法后施行超声乳化及折叠人工晶状体植入术的安全性和手术技巧。方法:23例28眼小瞳孔白内障,其中23眼为陈旧性虹膜睫状体炎,5眼为眼外伤术后,术中用维纳斯剪沿瞳孔缘连续环形剪除部分瞳孔区虹膜,后常规进行白内障超声乳化及折叠人工晶状体手术。结果:术后随访3～24mo,术后所有眼视力较术前提高(P<0.01),视力≥0.3者21眼,所有眼瞳孔保持圆形或近圆形、光反应存在,无严重并发症出现。结论:对于严重粘连小瞳孔白内障采用瞳孔缘连续环形剪除后再行白内障超声乳化及折叠人工晶状体植入术,方法简单,术中操作顺利,术后瞳孔形态正常或近于正常、光反射存在,眼前节反应轻,是一种较好的手术方式。     

Acute lens opacity induced by different kinds of anesthetic drugs in mice

AIM: To study whether specific anesthetic drugs or tear layer evaporation was primarily responsible for the acute cataract and what the change of lens structure is in anesthetized mice. METHODS: Five groups were set up in the experiment: Group A (topicamide and phenylephrine mixed eye drop+ chloral hydrate), Group B (tropicamide and phenylephrine mixed eye drop+sevoflurane), Group C (tropicamide and phenylephrine mixed eye drop), Group D (topicamide and phenylephrine mixed eye drop+chloral hydrate, carbomer eye drop in the right eyes), and Group E (tropicamide and phenylephrine mixed eye drop+sevoflurane, carbomer eye drop in the right eyes). A simple classification system was used to assess the severity of lens opacity. And a numerical value from 0 to 3 to each grade was assigned for the cataract index calculation and data analysis. The gross appearance and time course of development of lens opacity were assessed. Hematoxylin and eosin staining was used to observe the lens structure changes in the reversible cataract. RESULTS: Tropicamide did not induce lens opacification in mice. Lens opacity caused by inhaled sevoflurane was similar to injected cholral hydrate. Both inhaled-anesthetic-induced lens opacity and injected-anesthetic-induced lens opacity could be prevented by carbomer eye drop. In the severe opacity lens, a wide range of lens fiber cell structure had disordered. The fiber cells became uneven thickness. CONCLUSION: The acute reversible lens opacity can unilaterally develop or be induced by a local cause. The structure of lens fiber cells changed in the lens opacity which may influence the permanent connection of the lens fiber cells. This study was not only of practical significance to help maintain lens transparency for eye research, but also of the deeper consideration about the reversible lens opacification phenomenon.     

Prevalence of lens opacity in Chinese residents of Singapore: the tanjong pagar survey

OBJECTIVE: To determine the prevalence of lens opacity among Chinese residents of Singapore. DESIGN: Population-based, cross-sectional survey. PARTICIPANTS: Chinese men and women aged 40 years or more in Singapore. METHODS: A stratified, clustered, random sampling method, with more weights given to the older age groups, was used to initially select 2000 Chinese persons aged 40 to 79 years from the 1996 electoral register in the Tanjong Pagar district in Singapore. Eligible subjects (n = 1717) were invited for a comprehensive ocular examination at a centralized clinic, after which nonrespondents were examined in their homes with portable instruments. MAIN OUTCOME MEASURES: Lens opacity, as determined clinically at the slit lamp using a modification of the Lens Opacity Classification System III. Prevalence rates were age adjusted to the 1997 Singapore census population. RESULTS: Of the 1232 persons examined (71.8%), 1206 (70.2%) provided lens data for this analysis. The age-adjusted prevalence of any cataract surgery was 5.1% (95% confidence interval [CI], 3.6, 6.5), with similar rates between men and women. The age-adjusted prevalence of specific types of lens opacity was 22.6% (95% CI, 19.8, 25.4) for any nuclear opacity, 23.9% (95% CI, 21.0, 26.8) for any cortical opacity, and 7.0% (95% CI, 5.3, 8.8) for any posterior subcapsular opacity. The overall age-adjusted prevalence of any cataract or cataract surgery was 34.7% (95% CI, 31.5, 38.0), increasing from 7.0% in those 40 to 49 years and to 94.3% in those 70 years and older (P < 0.001, test for trend for age). When the prevalence of distinct types of lens opacity in an eye was considered, the most common was mixed (age-adjusted prevalence, 11.5%, based on right eye data), followed by nuclear only (9.7%), cortical only (8.3%), and posterior subcapsular only (0.6%). For all cataract types, the prevalence was similar in men and women and was higher in persons examined at their homes than in those examined at the study clinic. CONCLUSIONS: These population-based data suggest that cataract is common among adult Chinese residents in Singapore, despite ready access to cataract surgical services. Persons accepting the offer of free eye examinations were less likely to have cataract than those who did not take up this offer.     

Philly mouse: A new model of hereditary cataract

Philly mouse is a new strain of mice derived from the Swiss-Webster strain which develops hereditary cataracts visible to the naked eye ca. 5–6 weeks after birth. Slit lamp examinations of the apparently clear lenses of 15 day mice indicate the presence of faint anterior opacities which progress, involving the suture area, by day 25. By 30 days a nuclear opacity develops which surrounds the nucleus by day 35. At the same time the anterior subcapsular opacity becomes diffuse and pronounced as the cataract becomes obvious to the naked eye.Biochemical studies indicate that an osmotic cataract is formed in the Philly mouse. By ca. 20 days of age there is an increase in lens water along with an alteration in electrolyte levels. Lenticular sodium rapidly increases while potassium levels decrease. Concomitant with cataract formation is an increase in total lenticular calcium and a decrease in lens dry weight, reduced GSH and ATP.In transport studies, no significant difference between the Philly and control lens was seen in the accumulation of AIB. When rubidium was substituted for potassium a decreased accumulation in the Philly lens older than 20 days was correlated with increased rubidium leak-out. This decreased accumulation due to increased leak-out appears to be the key biochemical change that accounts for osmotic cataract formation and it suggests the possibility of a defect in membrane permeability.     

Eaf2基因敲除对紫外线诱导的鼠白内障形成的影响

目的：分析Eaf2基因敲除对紫外线诱导的小鼠白内障形成的影响。方法：将15只野生型（ WT）小鼠作为对照组,10只Eaf2基因敲除（ Eaf2 KO）小鼠作为实验组。两组均取14周龄左右的小鼠作为研究对象。进一步分组为：WT－nonUV、WT－UV、Eaf2 KO－nonUV、Eaf2 KO－UV,共4组。使用裂隙灯显微镜观察小鼠白内障程度,利用晶状体混浊分类系统Ⅱ（ LOCSⅡ）对小鼠白内障进行分级。然后断颈处死小鼠,摘取晶状体进行暗视野显微镜照相,利用Image J软件对晶状体混浊程度进行分析,并将各测量结果进行统计学处理。结果：裂隙灯显微镜和暗视野显微镜的结果一致：WT－UV组及 Eaf2 KO－UV 组晶状体混浊程度明显高于 WT－nonUV组及Eaf2 KO－nonUV组,其中WT－UV组明显高于Eaf2 KO－UV组,均具有统计学差异（P＜0．05）。结论：紫外线辐射能够导致小鼠白内障的形成,Eaf2蛋白质具有促进紫外线所致的小鼠白内障形成的作用。     

Progression of eye disease in "cured" leprosy patients: implications for understanding the pathophysiology of ocular disease and for addressing eyecare needs

BACKGROUND: Ocular damage in leprosy is due either to nerve damage or infiltration by mycobacteria. There is currently little information about the magnitude and nature of incident ocular pathology in cured leprosy patients. This information would increase our understanding of the pathophysiology of ocular involvement in leprosy and help in developing programmes to address the eyecare needs of leprosy patients who have been released from treatment. The cumulative incidence of leprosy related ocular pathology and cataract was measured during an 11 year follow up period in cured leprosy patients released from treatment in Korea. METHODS: In 1988 standardised eye examinations were performed on 501 patients in eight resettlement villages in central South Korea. In May 1999 standardised eye examinations were repeated in this population. RESULTS: Among the patients in whom there was no sight threatening leprosy related ocular disease (lagophthalmos, posterior synechia, or keratitis) in 1988, 14.7% developed one or more of these conditions. Overall, among those with no vision reducing cataract in 1988, 26.4% had developed a vision reducing lens opacity in at least one eye. Among patients examined in both 1988 and 1999, 14.3% developed visual impairment and 5.7% developed blindness. CONCLUSION: This study demonstrates that leprosy related ocular pathology progresses in some patients even after they are cured mycobiologically. The progressive leprosy related lesions are the result of chronic nerve damage; ocular lesions due to infiltration by Mycobacterium leprae did not develop. Based on the factors found to be associated with development of the most visually significant findings (posterior synechia, keratitis, and cataract) certain patients should be targeted at discharge for active follow up eye care. We suggest that patients with lagophthalmos (even in gentle closure), trichiasis, small pupils, and posterior synechiae should be screened regularly for the development of lagophthalmos in forced closure, keratitis, and cataract.     

Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice

We investigated whether decreased vitamin C (VC) in a mouse model increases lens opacity (cataract) induced by in?vivo exposure to ultraviolet radiation type B (UVR-B). Senescence marker protein-30 (SMP30) knockout (KO) mice, which cannot synthesize VC due to genetic disruption of the gluconolactonase (GNL) gene, were divided into 2 groups: VC sufficient (VC (+)) and VC deficient (VC (-)). Starting at 1 month of age, these groups had free access to water containing 0.0375 and 1.5?g/L of VC, respectively. SMP30 KO VC (-), SMP30 KO VC (+), and wild-type (WT) mice, all 14 weeks of age, were unilaterally exposed in?vivo to UVR-B (200?mW/cm(2)) for 100?s twice a week for 3 weeks (total: 1200?mJ/cm(2)). At 48?h after the last UVR-B exposure, cataract morphology was documented, and the ratio of cataract induction was quantified as the cataract area ratio (opacity area/anterior capsule). UVR-B exposure induced cataract mainly at anterior sub-capsular in SMP30 KO VC (-), SMP30 KO VC (+), and WT mice. In SMP30 KO VC (-) lenses the opacities were more extensive than in SMP30 KO VC (+) or WT lenses (cataract area ratios: 59.3%?±?10% vs. 32.2%?±?11.7% or 29.0%?±?9.0%; P?相似文献   

儿童外伤性白内障人工晶状体固定性瞳孔夹持的原因与手术治疗

目的 回顾性分析儿童外伤性白内障摘除术后人工晶状体(IOL)固定性瞳孔夹持的原因,并探讨有效的治疗方法.方法 对14岁以下外伤性白内障的患者行白内障摘除后房犁人工晶状体植人术后固定性瞳孔夹持患者10例(10只眼),分析其发生原因及并发症,并行虹膜粘连分离,后发性白内障切除,人工晶状体复位术及前段玻璃体切割术.5只眼行IOL置换术.结果 10只眼成功完成分离虹膜与囊膜粘连并行后发性白内障切除和人工晶状体复位,术后视功能不同程度的改善.结论 外伤性白内障术后葡萄膜炎反应,瞳孔粘连,后发性白内障及周边皮质再生是引起儿童IOL固定性瞳孔夹持的重要原因.手术分离虹膜粘连、清除晶状体再生皮质并切除后发性白内障可有效复位IOL.     

人及小鼠白内障晶状体上皮细胞凋亡的超微结构

目的 探讨人老年性白内障及小鼠先天性白内障与其晶状体上皮细胞凋亡的关系。方法 用透射电镜观察４例老年性白内障患者及２例先天性白内障小鼠晶状体上皮细胞凋亡的超微结构,并分别与同类正常晶状体上皮细胞超微结构进行比较。结果 老年性白内障患者及先天性白内障小鼠晶状体上皮细胞中均可见凋亡细胞,而正常晶状体未见凋亡细胞。结论 人老年性白内障及小鼠先天性白内障的发生均与其晶体上皮细胞的凋亡有关。     

特殊表型先天性白内障小鼠晶状体中细胞缝隙连接蛋白的表达

目的观察γD-晶状体蛋白点突变导致的小鼠先天性白内障表型,检测该特殊表型先天性白内障小鼠晶状体中细胞缝隙连接蛋白（Cx）的表达。方法观察突变小鼠出生后不同时间晶状体的形态学变化;应用免疫荧光染色法分析晶状体内Cx46和Cx50的表达和分布。结果突变小鼠模型呈稳定一致的显性遗传,出生后7 d即表现为明显的核性白内障,出生后21 d纯合子小鼠晶状体混浊严重,后囊膜自然破裂;免疫荧光染色分析发现突变小鼠晶状体内Cx46和Cx50的表达均出现下降,越靠近晶状体中心部下降越明显。结论γD-晶状体蛋白点突变可导致晶状体后囊膜破裂这种特殊表型的先天性白内障,白内障的形成和后囊膜破裂的产生与晶状体内Cx46和Cx50的表达下降有关。     

儿童人工晶状体固定性瞳孔夹持的原因和手术复位

目的　分析儿童白内障摘除术后人工晶状体 (IOL)固定性瞳孔夹持的原因 ,并探讨有效的处理方法。方法　收集年龄 <14岁、白内障摘除后房型IOL植入术后发生固定性瞳孔夹持患者 30例 (31只眼 )的临床资料 ,分析其发生原因和并发症 ,并对其中 8例 (8只眼 )合并严重并发症患者行IOL复位手术。结果　全部患者均发生较严重的葡萄膜炎性反应及其所致的虹膜后粘连和瞳孔膜闭或闭锁 ,以及不同程度的后发性白内障。IOL复位手术中可见 8例患者均发生不同程度的晶状体周边皮质再生。结论　白内障摘除术后葡萄膜炎性反应、晶状体皮质再生及后发性白内障是引起儿童IOL固定性瞳孔夹持的主要原因。手术分离虹膜粘连、清除晶状体再生皮质并切除后发性白内障 ,可有效复位IOL。     

玻璃体切除联合白内障手术保留晶状体前囊或后囊的观察比较

目的观察玻璃体视网膜疾病伴有白内障需行玻璃体切除时保留品状体前囊和后囊的临床反应和疗效。方法30例（30眼）玻璃体视网膜疾病伴有白内障者,随机分成两组,每组15例（15眼）。A组（保留品状体后囊）行小切口白内障摘出后再行玻璃体切除,B组（保留晶状体前囊）行常规闭合式三通道手术,先在晶状体前囊下切除晶状体再行玻璃体切除术。结果术后视力均有不同程度提高,术后1周,眼压升高者：A组4例（4眼）,B组1例（1眼）;虹膜后粘连者：A组5例（5眼）,B组0例。术后1～2个月,虹膜后粘连者：A组15例（15眼）均出现程度不一的虹膜后粘连,9例因瞳孔闭锁,虹膜高度膨隆出现继发性青光眼;B组0例且眼压均正常。结论玻璃体切除合并白内障保留晶状体前囊可以避免虹膜后粘连及其引起的继发性青光眼,并减轻了眼部组织的损伤。     

Nuclear cataract and myopic shift in refraction

PURPOSE: Nuclear cataract is thought to cause a myopic shift in refraction in older persons. The authors tested this hypothesis by assessing the correlation of nuclear opacity with spherical equivalent refraction (SER) in an older population. DESIGN: Population-based cross-sectional study. METHODS: Three thousand six hundred and fifty-four participants aged 49 years or older were examined in the Blue Mountains Eye Study [BMES] (1992 to 1994). Eye examinations included subjective refraction and lens photographs. Nuclear opacity was assessed against four standard slit-lamp lens photographs using the Wisconsin Cataract Grading System. Nuclear cataract was defined as opacity level 4 or higher. RESULTS: Participants without nuclear cataract had an annual mean hyperopic shift of 0.05 diopters (P < .0001). In persons with nuclear cataract, this hyperopic shift disappeared. A myopic refractive shift occurred only in persons with nuclear opacity levels of 4 or higher. CONCLUSION: This study documents the contribution of nuclear cataract to the mild myopic shift that neutralizes the age-related hyperopic shift occurring in older persons.     

波形纤维蛋白在其转基因小鼠晶状体的表达

目的 观察波形纤维蛋白（Ｖｉｍｅｎｔｉｎ）在转基因小鼠晶状体的表达,探讨波形纤维蛋白与内障形成的关系。方法 用显微注射法将鸡的１２．７ｋｂ波形纤维蛋白基因导入小鼠受精卵的雄前核内,经培育、杂交传代得到２０只波形纤维蛋白内障小鼠。取４只白内障眼球及４只正常眼球,制备石蜡切片,采用ＡＢＣ免疫组化方法,观察波形纤维蛋白在晶状体的表达。再分别正常、分部混浊和完全混浊的小鼠晶状体各１只,经ＳＤＳ－聚丙烯酰胺