Bidirectional communication of sensory afferent information in a peripheral nerve of the cat forelimb

Kinaesthetic information is derived from both muscle and joint nerves. However, the segregation, at peripheral levels, of inputs from these sources is by no means clear cut. In the present report, we demonstrate the complexity of peripheral innervation of joint and muscle structures in the cat’s forearm, in particular, with evidence for bidirectional signalling for different classes of kinaesthetic afferents within a peripheral nerve segment. Three-way simultaneous recordings were carried out in the anaesthetized cat from single kinaesthetic afferents in three nerves that were freed from nearby tissue in the distal forearm, but remained in continuity. These were the wrist-joint nerve and two components of the indicis proprius nerve, one that projects proximally from the muscle to join the deep radial nerve, the other a distal extension of this nerve that runs through and beyond its own muscle to the region of the wrist-joint capsule where it forms an anastomosis with the wrist-joint nerve. Single-unit recording from the intact nerves demonstrated that some spindle afferent fibres from the indicis proprius muscle may take an ”ectopic” path to the central nervous system, conveying their signals over an initial centrifugal path via the distal extension of the indicis proprius nerve, before looping back to project centripetally via the ”classic” wrist-joint nerve. As some wrist-joint afferents themselves may project ”ectopically” via the distal and then proximal segment of the indicis proprius nerve (rather than via the wrist-joint nerve), the recordings demonstrate that, within the distal segment of the indicis proprius nerve, there is bidirectional traffic of kinaesthetic afferent signals, with wrist-joint impulses travelling centripetally and muscle afferent signals travelling centrifugally. The findings emphasize the complexity of signalling that may be present in sensory nerves, on account of the ”ectopic” paths taken by some afferents, and the need to activate deep inputs of joint or muscle origin by natural stimulation of the appropriate receptors in order to examine selectively the central actions and processing of either source of input. Received: 8 December 1998 / Accepted: 7 May 1999     

Quantitative analysis of peptide levels and neurogenic extravasation following regeneration of afferents to appropriate and inappropriate targets

We have studied quantitatively the levels of substance P and calcitonin gene-related peptide in nerves innervating skin and muscle of rats, and examined the effects of cross-anastomosing these nerves so that they regenerate to an inappropriate target. We have also compared the ability of nerves to induce neurogenic extravasation with their peptide content. Peptide was measured by radioimmunoassay in the proximal section of ligated peripheral nerves, and neurogenic oedema was measured by determination of Evans Blue extravasation induced by either systemic capsaicin treatment or topical mustard oil application. The levels of these peptides are higher in cutaneous nerves than muscle nerves. This cannot be explained by differences in the number of fibres in the nerves studied. The levels of peptides fall when cutaneous afferents reinnervate muscle, and rise when muscle afferents reinnervate skin. We suggest that these changes occur because of some tissue-specific trophic influence arising from the tissue innervated. The ability to produce extravasation in skin is highly correlated with the substance P and calcitonin gene-related peptide levels of its innervation, even when this occurs in inappropriate nerves which do not normally produce extravasation.     

Distribution of synaptic field potentials induced by TTX-resistant skin and muscle afferents in rat spinal segments L4 and L5

Previous results from our group and others showed that skin and muscle afferents are equipped with tetrodotoxin-resistant (TTX-r) channels. The great majority of the TTX-r fibres are unmyelinated (C or group IV) and are assumed to have nociceptive functions. Therefore, a block of the TTX-sensitive (TTX-s) fibres offers the possibility to study reactions of central nervous neurones to a purely nociceptive input. The present study compared spinal synaptic field potentials (SFPs) evoked by electrical stimulation of TTX-r afferent fibres from skin and muscle at various depths of the spinal segments L4 and L5 in the rat. Cutaneous input was produced by stimulation of the sural nerve (SU), input from muscle by stimulation of the gastrocnemius-soleus nerves (GS). To block the (non-nociceptive) TTX-s afferents, a pool containing TTX (concentration 1microM) was built around the dorsal roots L3-L6. As a measure of synaptic activity, the area of averaged SFPs was determined. After TTX application, the SFPs of fast conducting myelinated afferent fibres vanished completely. Simultaneously, the size of the potentials evoked by electrical stimulation of slowly conducting TTX-r skin and muscle afferents increased significantly. The field potentials of TTX-r GS afferents had a maximum in laminae IV-VI of the dorsal horn, whereas the SFPs induced by SU stimulation were more evenly distributed over all laminae. The results are a further indication that nociceptive input from skin and muscle is differently processed at the spinal level.     

The organization of primary afferent depolarization in the isolated spinal cord of the frog

1. The organization of primary afferent depolarization (PAD) produced by excitation of peripheral sensory and motor nerves was studied in the frog cord isolated with hind limb nerves.2. Dorsal root potentials from sensory fibres (DR-DRPs) were evoked on stimulation of most sensory nerves, but were largest from cutaneous, joint and flexor muscle afferents. With single shock stimulation the largest cutaneous and joint afferent fibres gave DR-DRPs, but potentials from muscle nerves resulted from activation of sensory fibres with thresholds to electrical stimulation higher than 1.2-1.5 times the threshold of the most excitable fibres in the nerve. This suggests that PAD from muscle afferents is probably due to excitation of extrafusal receptors.3. Dorsal root potentials produced by antidromic activation of motor fibres (VR-DRPs) were larger from extensor muscles and smaller or absent from flexor muscles. The VR-DRPs were produced by activation of the lowest threshold motor fibres.4. Three types of interactions were found between test and conditioning DRPs from the same or different nerves. With maximal responses occlusion was usually pronounced. At submaximal levels linear summation occurred. Near threshold the conditioning stimulus frequently resulted in a large facilitation of the test DRP. All three types of interactions were found with two DR-DRPs, two VR-DRPs or one DR-DRP and one VR-DRP.5. The excitability of sensory nerve terminals from most peripheral nerves was increased during the DR-DRP. The magnitude of the excitability increase varied roughly with the magnitude of the DR-DRP evoked by the conditioning stimulus.6. There was a marked excitability increase of cutaneous and extensor muscle afferent terminals during the VR-DRP. Flexor muscle afferent terminals often showed no excitability changes to ventral root stimulation. In those experiments where afferent terminals from flexor muscles did show an excitability increase, the effects were smaller than those of cutaneous and extensor terminals.7. The VR-DRPs appear to reflect activity of a negative feed-back loop from extensor motoneurones on to sensory fibres from cutaneous and extensor muscles. This system may have a role in modulating the ballistic movement of the frog. DR-DRPs, on the contrary, are widespread in origin and distribution. PAD from sensory fibres may function to sharpen contrast between incoming afferent information.     

Substance P-like immunoreactivity in cultured spinal ganglia from chick embryos

Summary The localization of substance P (SP) or a SP-like peptide in cultured spinal ganglia from chick embryos was studied by the indirect immunofluorescence technique. Ganglia from 8–16 days old chick embryos and from newly hatched chickens were cultured in a control medium or in the presence of nerve growth factor (NGF). Addition of colchicine and exposure to different explanted peripheral tissues were also tried. Ganglia from the younger embryos (8–12 days) cultured for 24 h with added NGF showed a weak SP-like immunoreactivity (SPLI) in some cell bodies and strong specific immunofluorescence in nerve fibres growing out from the ganglia. In spinal ganglia of the older embryos (14 and 16 days) and newly hatched chickens cultured with and without NGF the concentration of SPLI in the cell bodies was considerably higher. Addition of colchicine to spinal ganglia cultured 12 h in NGF-medium, resulted in retraction of nerve fibres and strongly fluorescent, expanded nerve fibres were observed in peripheral parts of the ganglia. Explants of skin placed near the spinal ganglia stimulated the outgrowth of fibres, some of them containing SPLI. A few fluorescent fibres were also seen within the skin explants. Also heart tissue explants stimulated outgrowth of nerve fibres, but innervation of these explants with SPLI-containing nerves could not be observed. Nerve fibre-extension from the spinal ganglia was not stimulated by spinal cord explants. The present results support the existence of SP-containing primary sensory neurons in chickens.     

Neck muscle afferent input to spinocerebellar tract cells of the central cervical nucleus in the cat

Summary Extracellular and intracellular recordings were made from spinocerebellar tract neurones of the central cervical nucleus (CCN) in C1–C3 segments of the anaesthetized cat. These neurones were identified by antidromic activation from the cerebellar peduncle. Stimulation of the ipsilateral dorsal root elicited extracellular spikes or EPSPs with a monosynaptic latency in almost all CCN neurones in the same segment (segmental input). Late excitatory effects were also observed in about one third of CCN neurones. The monosynaptic EPSP was occasionally followed by an IPSP. The excitatory input from the dorsal root to CCN neurones was extended over several segments for some CCN neurons (extrasegmental input). Monosynaptic excitation was evoked in CCN neurones after stimulation of dorsal neck muscle nerves as well; i.e. splenius (SPL), biventer cervicis and complexus (BCC), rectus capitus dorsalis, and obliquus capitus caudalis. Thresholds for this excitation were near the threshold of the nerve, suggesting that it originated from group I fibres. The component of excitation added after strong stimulation of neck muscle nerves would be attributed to group II fibres. When a CCN neurone received excitatory input from the nerve of one muscle, it was generally not affected by stimulation of other nerves in the same segment. Such muscle specificity of segmental input was the principal pattern of connexion of neck muscle afferents with CCN neurones. In some cases, however, excitatory convergence from SPL and BCC nerves onto single CCN neurones or excitation from the SPL nerve and inhibition from the BCC nerve were also observed. Nearly half of the CCN neurones received input from one muscle nerve of the same segment and not from the afferent of the same muscle of different segments, indicating a segment specificity of input. In the remaining CCN neurones, weaker excitatory effects were induced from afferents of different segments as well. In such extrasegmental effects, inputs to CCN neurones from caudal segments predominated in frequency over those from rostral segments. The origin of extrasegmental input was generally confined to the same muscle. Low threshold muscle afferents from the SPL and BCC were intraaxonally stained with HRP. The collaterals of the stained fibre distributed branchlets and terminals to the CCN, laminae VII, VIII, and motor nuclei. Two fibres responding to local muscle prodding or stretch showed a similar morphology. The findings indicated that muscle spindle afferents from primary endings projected to the CCN.Supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan     

The response of some sympathetic neurones to volleys in various afferent nerves

1. Responses of sympathetic neurones to various afferent inputs have been analysed both in anaesthetized cats with intact neuraxis and in spinal cats.2. In anaesthetized cats electrical stimulation of low threshold group III afferent fibres in skin and muscle nerves inhibited sympathetic neurones and gave depressor responses. The silent period following a sympathetic reflex discharge is most likely due to inhibition brought on by a particular subgroup within this afferent group.3. High threshold group III and group IV afferent fibres excited sympathetic neurones and elicited pressor responses.4. Sympathetic reflex arcs could be temporally facilitated during the somatic afferent induced inhibition, by group IV, and to a lesser extent by group III volleys.5. Section of the spinal cord shortened the time course and lessened the degree of group III inhibition suggesting that suprasegmental pathways are involved in the long-lasting depression following a reflex.6. Baroreceptor afferent stimulation inhibited group III- and group IV- evoked reflexes even with some temporal facilitation in the pathway.7. The special properties of the sympathetic reflex arcs and the relation of the results to other work on B.P. reflexes are discussed. It is suggested that the group III and IV muscle afferents have a chemoreceptor function and are responsible for mediation of the pressor reflex during muscle exercise.     

Differences in the chemical expression of rat primary afferent neurons which innervate skin, muscle or joint

The fluorescent dye Fast Blue was injected in anaesthetized rats into either skin, muscle or knee joint of the hindlimb. Following retrograde transport of the dye to lumbar dorsal root ganglia, the cell bodies of primary afferent neurons innervating these different target tissues were identified in ganglion sections by fluorescence microscopy. The sections were processed to demonstrate activity of the enzyme thiamine monophosphatase, or immunoreactivity to calcitonin gene-related peptide, substance P, or somatostatin, in Fast Blue labelled neurons. In all cases immunoreactivity to the antineurofilament antibody RT97 was used to classify dorsal root ganglion cells as being either small dark (RT97 negative, unmyelinated axons) or large light (RT97 positive, myelinated axons). The proportion of small dark cells labelled from each target decreased in the order: skin, muscle, joint. Thiamine monophosphatase and somatostatin were present only in small dark cells, while calcitonin gene-related peptide and substance P were found in both small dark and large light cells. In large light cells of all three targets, more contained calcitonin gene-related peptide than substance P. Among small dark cells, thiamine monophosphatase and somatostatin were found predominantly in skin afferents, while calcitonin gene-related peptide and substance P were more common in muscle and joint afferents. The chemical expression of primary afferents is therefore characteristic of the peripheral target they innervate. This could reflect either a maintained influence of the target on the afferents, or the factors which operate only during development.     

Regeneration of afferent and efferent fibres to muscle spindles after nerve injury in adults cats.

1. The nerves to cat peroneus longus and tenuissimus muscles were either cut or crushed close to the muscle and the afferent and efferent nerve supply to the muscle spindles was studied electrophysiologically between 2 and 32 weeks later. 2. Recovery was more rapid and complete after crush than section for both afferent and efferent fibres. After recovery from either procedure normal primary and secondary afferents and static and dynamic gamma efferent fibres were found. 3. Some abnormally occurring neurones were found. One group consisted of beta fibres which had a static action on muscle spindles. Static beta fibres are very rarely found in normal muscles. 4. The results indicate that some guidance mechanism exists which after crush injuries of nerves may restore muscle receptor function almost to normal. Even after nerve section some muscle spindles may become correctly reinnervated.     

Innervation of facial skin but not masticatory muscles or the tongue by trigeminal primary afferents containing somatostatin in the rat

Using retrograde transport of a fluorescent dye, True blue, the peripheral tissues innervated by trigeminal ganglion neurones containing somatostatin have been investigated. Of ganglion neurones retrogradely labelled from injections of dye into the facial skin, 3.45% were found to be immunoreactive for somatostatin. In contrast, none of the neurones labelled from injections of dye into the tongue or masseter muscle were found to contain this peptide. This demonstration of a restricted distribution of somatostatin-containing primary afferents raises the possibility that somatostatin may be involved in functions which are specific to skin and not to the other tissues examined.     

Clustering of Pacinian corpuscle afferent fibres in the human median nerve

 To further study the functional organisation of human peripheral nerves, the intrafascicular arrangement of afferent fibres supplying Pacinian corpuscles (PCs) was explored by percutaneous microneurography using thin-calibre, concentric needle electrodes. In normal adults, 20 PC afferents were identified in 13 recording sites. Low-amplitude (less than 30 μm) vibratory stimuli to the skin were applied with tuning forks oscillating at 128 Hz or 256 Hz and response patterns of individual PC units were studied. In many recording sites, two, sometimes even three, PC afferents with adjacent or overlapping receptive fields in the hand were clustered in the nerve. The observed incidence in the records containing a certain number of PC units was compared with the expected probability calculated according to the hypothesis that all nerve fibres are randomly organised in peripheral nerves. The results suggested that PC afferents are partially segregated in the nerve. In addition, PC afferents were neighbouring on slowly adapting type II (SAII) units and skin sympathetic activity in individual fascicles. SAII units often innervated the same skin area as PC units, but did not respond to vibration. The data provided additional information regarding the functional organisation of the human peripheral nerve and the mechanisms underlying the sense of vibration in man with special regard to population behaviour of neighbouring PC mechanoreceptors. Received: 25 September 1998 / Accepted: 14 December 1998     

Modulation of gastro-oesophageal vagal afferents by galanin in mouse and ferret

The neuropeptide galanin is found in the central and peripheral nervous systems. It may have excitatory or inhibitory actions via three subtypes of G-protein-coupled receptor, and it modulates the mechanosensitivity of somatic sensory fibres. We aimed to determine if galanin also modulates vagal afferent mechanosensitivity, and to localize endogenous sources. The responses of ferret and mouse gastro-oesophageal vagal afferents to graded mechanical stimuli were investigated in vitro. The effects of galanin and/or the galanin receptor antagonist galantide on these responses were quantified. Immunohistochemistry for galanin was performed in ferret and mouse proximal stomach and nodose ganglion. In ferrets, retrograde labelling of gastric afferents to the nodose ganglion was combined with immunohistochemistry. When exposed to galanin (1–10 n m ), 18/31 ferret and 12/15 mouse gastro-oesophageal afferents (tension, mucosal and tension/mucosal receptors) showed inhibition of mechanosensitivity. Four of 31 ferret afferents showed potentiation of mechanosensitivity, and 9/31 were unaffected (2/15 and 1/15 in mouse, respectively). Galanin effects were reversed after washout or by galantide (10–30 n m ). Galantide given alone increased mechanosensitivity. Galanin immunoreactivity was found in nodose neurones, including those innervating the stomach in ferret. Enteric neurones were also galanin immunoreactive, as were endings associated with myenteric ganglia and smooth muscle. We conclude that galanin potently modulates mechanosensitivity of gastro-oesophageal vagal afferents with either facilitatory or inhibitory actions on individual afferent fibres. Both intrinsic and extrinsic (vagal) neurones contain galanin and are therefore potential sources of endogenous galanin.     

Afferent connexions to Group I activated cells in the main cuneate nucleus of the cat

1. Extracellular recordings were made from a total of 240 group I activated cells in the main cuneate nucleus. Cuneothalamic relay neurones (128) were identified by antidromic stimulation of the medial lemniscus in the ventrobasal thalamic complex.2. A majority of the relay neurones were activated by afferents in only one of six dissected forelimb nerves innervating muscle groups at various joints. Even among afferents from adjacent synergistic muscles, convergence to individual neurones was infrequent.3. Some of the relay neurones received excitation from group II muscle afferents in the same nerve that provided group I excitation. Excitation from group II muscle afferents in other nerves was uncommon. Some neurones were weakly excited by cutaneous volleys.4. Inhibition of group I relay cells was produced from cutaneous afferents and group II muscle afferents. Weak inhibition was sometimes observed from group I afferents. The relay cells were also inhibited by stimulation of the cerebral cortex with a focus around the lateral end of the cruciate sulcus. A good correspondence was found between the inhibition and the depolarization of group I afferent terminals in the cuneate nucleus.5. A majority of the group I activated cells not antidromically activated from the ventrobasal complex (;non-relay cells') were excited by cortical stimulation. Excitation from cutaneous afferents and group II muscle afferents was frequently found among these cells.6. The group I activated cells were found almost exclusively in the ventral part of the nucleus.7. The pattern of convergence found in eleven group I activated cells in the dorsal horn of the spinal cord from C 2 to C 4 is described.     

Distribution of histamine in the developing peripheral nervous system

The presence and ontogenetic distribution of histamine was studied in the developing peripheral nervous system of the rat by using an indirect immunofluorescence technique and a specific rabbit anti-histamine antiserum. Histamine immunoreactivity (IR) first appeared in peripheral nerves on embryonic day 14. The number and intensity of histamine-immunoreactive nerves was highest on embryonic days 16–18. During development starting from embryonic day 14, motoneurones in ventral horns of the spinal cord at cervical, thoracic and lumbar levels contained histamine IR. A subpopulation of sensory neurones in dorsal root ganglia exhibited histamine IR. Histamine IR was also present in nerve fibres of ventral and dorsal roots of spinal cord, as well as in spinal nerves. Population of neurones and nerve fibres in sympathetic and pelvic ganglia as well as in myenteric ganglia of the intestine were also labelled with the histamine antiserum. In peripheral target organs, histamine IR was observed in nerve fibres around bronchi of the lungs, in the atria of the heart, in the adrenal gland, in the intestinal wall, in muscular tissues and in subepithelial tissue of the skin.The results of this study indicate that histamine is widely distributed in different types of neurones and nerve fibres of the developing peripheral nervous system.     

Co-localization of calcitonin gene-related peptide- and substance P-like immunoreactivity in mucosal intra-epithelial nerves in the toad colon.

Mucosal intra-epithelial nerve terminals have been found in the colon of the toad, Bufo marinus. Co-localized in the fibres are calcitonin gene-related peptide (CGRP)-like immunoreactivity (LI) and substance P (SP)-LI. The intraepithelial nerves are likely to be terminals of primary afferent sensory neurones. Fibres with vasoactive intestinal peptide (VIP)-LI or neuropeptide Y (NPY)-LI also supply the mucosa but do not penetrate the epithelium.     

Painful and non-painful pressure sensations from human skeletal muscle

Painful and non-painful pressure sensations from muscle are generally accepted to exist but the peripheral neural correlate has not been clarified. The aim of the present human study was to assess the non-painful and painful pressure sensitivity with (1) anaesthetised skin, and (2) anaesthetised skin combined with a block of large diameter muscle afferents. The skin was anaesthetised by a topically applied anaesthetic cream and later lidocaine was administrated subcutaneously. The pressure sensitivity was assessed quantitatively by computer-controlled pressure stimulation on the anterior tibial muscle. Thresholds to detection, pain and pain tolerance were assessed. In the first experiment, computer-controlled needle insertion depths evoking touch and pain sensations were used to assess the efficacy of cutaneous anaesthesia. Touch and pain sensations evoked during needle insertions were found to be superficial in intact skin but when anaesthetised, touch sensation was occasionally evoked at depths related to penetration of the fascia. With the skin completely anaesthetised to brush and von Frey hair pinprick stimulation, skin indentation with the strongest von Frey hair caused a sensation described as a deep touch sensation. Simultaneously, pressure detection and pain thresholds increased but it was still possible to elicit non-painful and painful pressure sensation in all subjects. In a second experiment, a differential nerve block of group I and II afferent fibres was obtained by full-leg ischaemia simultaneously with cutaneous anaesthesia. The efficacy of the tourniquet block was continuously assessed by a battery of somatosensory tests (heat, brush, vibration, electrical and movement detection) applied at the foot simultaneously with pressure stimulation on the anterior tibial muscle. After 20 min of ischaemia, group II afferent fibres mediating the sensations of movement detection, vibration and brush on the foot was blocked but the heat pain threshold was not affected. In this condition (anaesthetised skin and block of group I and II fibres from deep tissue) a pressure sensation was evoked in 70% of subjects although the pressure detection threshold was increased. The pressure pain sensitivity was decreased, which, however, might indicate a partial block of group III and IV muscle afferents. In a third experiment, the tactile sensations elicited by electrical stimulation of the tibialis anterior muscle and skin at the lower leg were significantly decreased after 20 min of ischaemia, validating the blocking effects of group I and II nerve fibres. The present data show a marginal contribution of cutaneous afferents to the pressure pain sensation that, however, is relatively more dependent on contributions from deep tissue group III and IV afferents. Moreover, a pressure sensation can be elicited from deep tissue probably mediated by group III and IV afferents involving low-threshold mechanoreceptors.     

Distribution of calcitonin gene-related peptide-like immunoreactivity in the medulla oblongata of the cat, in relation to choline acetyltransferase-immunoreactive motoneurones and substance P-immunoreactive fibres

The immunohistochemical distribution of calcitonin gene-related peptide (CGRP)-like immunoreactivity (ir) in the cat medulla oblongata was examined using an antiserum to rat alpha-CGRP. Comparative distributions of substance P (SP)-like and choline acetyltransferase (ChAT)-like ir were also studied on sections adjacent to those stained for CGRP, and on sections simultaneously stained for CGRP by double staining techniques. The vast majority of ChAT-ir motoneurones in somatomotor or branchiomotor cranial nuclei (of VI, VII and XII nerves) and their accessory nuclei also displayed a coarsely granular CGRP-ir, shown by electron microscopic examination to represent immunoreactive Golgi bodies. The nucleus ambiguus (IX and X nerves), a mixed branchiomotor and visceromotor nucleus, showed CGRP-ir in a lower proportion of its motoneurones, whereas the purely viseromotor dorsal motor vagal nucleus (X nerve) contained no CGRP-ir cells. A few CGRP-ir but ChAT-negative cells were seen in the ventromedial reticular formation, lateral cuneate nucleus, infratrigeminal nucleus and nucleus of the solitary tract. Coarse, often varicose CGRP-ir fibres were most prominent in the X and IX cranial nerve rootlets, the spinal tract of the V nerve and the solitary tract, and also in the V spinal nucleus and nucleus of the solitary tract. Many of these also appeared to contain SP-ir. The central patterns of CGRP and SP-ir fibres thus reflect the previously reported coexistence of these peptides in sensory afferent cells of the trigeminal and nodose ganglia. These results are consistent with a role for CGRP as a transmitter or modulator in efferents to striated muscle, sensory afferents and intrinsic neurones in the cat brain stem.     

Afferent input from distal forelimb nerves to branching spinoreticular-spinocerebellar neurones in the cat

Patterns of afferent connections from receptors of the distal forelimb were investigated in neurones located in C6-C7 segments of the spinal cord with branching axons projecting to the lateral reticular nucleus and the cerebellum. Experiments were made on five adult cats under alpha-chloralose anaesthesia. After antidromic identification, EPSPs and IPSPs were recorded from 22 neurones following stimulation of deep radial, superficial radial, median and ulnar nerves. Both excitatory and inhibitory effects were found in the majority of the cells, however, in 2 cases no synaptic actions were recorded. EPSPs were evoked from group I or II muscle, or cutaneous afferents - mostly monosynaptically. IPSPs from muscle, cutaneous or flexor reflex afferents were mostly polysynaptic. Seven various types of convergence were established in the cells investigated. Significance of parallel transmission of integrated information from various receptors of the distal forelimb to the reticular formation and cerebellum is discussed.     

Regenerating sprouts of axotomized cat muscle afferents express characteristic firing patterns to mechanical stimulation.

1. In cats, we studied the physiological properties of regenerating sprouts of muscle afferent fibers and compared them with sprouts from cutaneous afferent fibers. 2. Muscle nerves to the triceps surae and cutaneous sural nerves were axotomized in the popliteal fossa, and the proximal ends were inserted into nerve cuffs. Six days later, we recorded action potentials from single Groups I and II muscle and mostly Group II cutaneous afferents driven by mechanostimulation of the cuff. 3. Most muscle afferent sprouts (91%) had a regular slowly adapting discharge in response to sustained mechanical displacement of the cuff, particularly to sustained stretch stimuli, whereas most cutaneous afferents (92%) did not. Muscle afferents were more likely to have a spontaneous discharge and afterdischarge. 4. Group II muscle afferent sprouts had lower stretch thresholds and a higher incidence of spontaneous discharge compared with Group I fiber sprouts, whereas Group I fibers had a higher incidence of high-frequency afterdischarge to mechanical stimuli. 5. We conclude that, 6 days after axotomy, regenerating sprouts of muscle afferents, particularly Group II afferents, have become mechanosensitive in the absence of a receptor target and exhibit physiological properties similar to those found when innervating their native muscle but significantly different from sprouts of cutaneous afferents. Expression of these native muscle afferent firing patterns after the inappropriate reinnervation of hairy skin may be due to inherent properties of the muscle afferent fiber.