口腔鳞癌中p12蛋白的表达

目的：探讨p12蛋白在口腔鳞状细胞癌组织中的表达及其与口腔鳞癌发生发展的关系。方法：采用免疫组织化学S-P法,对40例口腔鳞癌组织,4例重度异常增生,8例口腔粘膜溃疡,6例口腔正常粘膜组织中p12蛋白的表达情况进行检测。结果：口腔鳞癌及重度异常增生组织中p12蛋白的表达明显低于炎性病变及正常粘膜组织。结论：p12蛋白在口腔鳞状细胞癌组织中的表达常为缺失,说明其作为一种候选抑癌基因在口腔鳞癌的发生中可能起一定作用,且不存在组织部位特异性。     

Prognostic significance of p53 expression in oral squamous cell carcinoma without neck node metastases

Background. Aberrations of the p53 gene and overexpression of its protein are widely recognized markers of malignancy including oral squamous cell carcinomas. This study was performed to evaluate the relationship of immunoexpression of p53 protein in series of 91 squamous cell carcinomas of the oral cavity with clinicopathologic parameters and to investigate whether p53 immunoexpression might influence the clinical outcome of the disease. Methods. From a group of 287 consecutive patients, 91 surgically treated ones were randomly selected. P53 protein expression was investigated by means of immunohistochemistry. Clinical and histopathologic data were gathered, and the patient survival was analyzed. Results. Of the oral carcinomas, 52.7% (n = 48) overexpressed p53, using a threshold of 10% stained cell nuclei. There was a negative correlation of p53 immunoexpression with a histologic grade of differentiation (r = ?0.236, p = .06) but not with clinical variables. Overall survival rate was 59% at 5 years. In univariate analysis, tumor size, node status, and advanced clinical stage were significantly associated with shortened overall survival. In patients without neck node metastases, p53 showed a strong correlation with survival (p = .01). In multivariate analysis performed only on N0 patients, tumor extension and p53 immunoexpression were found to be the only independent prognostic parameters with relative risks of 1.9 and 4.3, respectively. Conclusions. A strong relationship was observed between p53 immunoexpression and poor prognosis in patients with oral squamous cell carcinomas without neck node metastases. © 2004 Wiley Periodicals, Inc. Head Neck 26: 22–30, 2004     

PTEN和p53在食管鳞癌中的表达及意义

目的探讨抑癌基因PTEN、p53在食管鳞癌中的表达及意义。方法应用免疫组化S-P法检测食管鳞癌组织、癌旁正常食管组织中PTEN和p53的蛋白表达情况,分析其在食管鳞癌发生发展过程中的作用以及两者之间的作用关系。结果PTEN、p53在食管鳞癌组织中的阳性表达率分别是53.33%(32/60)、61.67%(37/60),对照组阳性表达率分别是91.67%(55/60)、3.33%(2/60)。PTEN阳性表达与食管鳞癌的分化程度、淋巴结转移、浸润深度、TNM分期有关(P<0.05),与年龄、性别无关(P>0.05)。p53阳性表达与食管鳞癌的分化程度、淋巴结转移、浸润深度有关(P<0.05),与TNM分期、年龄、性别无关(P>0.05)。PTEN与p53在食管鳞癌中的表达呈负相关关系(P<0.05)。结论PTEN低表达p、53高表达在食管鳞癌的发生发展中起重要的作用,二者相互作用,诱导食管鳞癌的发生发展。联合检测PTEN、p53对食管鳞癌的诊断、治疗有指导意义。     

Tumor-specific genetic expression profile of metastatic oral squamous cell carcinoma

BACKGROUND: Metastasis is the most important predictor of survival in patients with oral squamous cell carcinoma (OSCC). We tested the hypothesis that there is a genetic expression profile associated with OSCC metastasis. METHODS: We obtained samples from 6 OSCC node-positive primary tumors and their matched metastatic lymph nodes, and 5 OSCC node-negative primary tumors. Using laser capture microdissection, we isolated OSCC cells from metastatic lymph nodes and compared them with those from matched primary tumors and unmatched node-negative primary tumors using Affymetrix Human Genome Focus arrays. RESULTS: Comparison of tumor cells from the lymph nodes with those from the unmatched, node-negative primary tumors revealed differential expression of 160 genes. Hierarchical clustering and principal component analysis using this 160-gene set showed that the node-negative samples were distinguishable from both, node-positive primary tumors and tumors in the lymph nodes. Many of the expression changes found in the metastatic cells from the lymph nodes were also found in the node-positive primary tumors. Immunohistochemical analysis for transglutaminase-3 and keratin 16 confirmed the differential genetic expression for these genes. CONCLUSION: These preliminary results suggest that there may be a metastatic gene expression profile present in node-positive primary OSCC.     

Cutaneous metastasis from esophageal basaloid squamous cell carcinoma: A case report

Introduction and importanceBasaloid squamous cell carcinoma (BSCC) of the esophagus is a relatively rare histologic variant of squamous cell carcinoma. Here, we reported a case of solitary cutaneous metastasis as the first symptom of esophageal BSCC and was successfully treated with multidisciplinary treatment.Case presentationA 67-year-old man visited a local hospital with symptoms of dysphagia and cutaneous nodules on his left shoulder. Fluorine-18 fluorodeoxyglucose positron emission tomography revealed hypermetabolic accumulations in the middle thoracic esophagus, right recurrent laryngeal nerve lymph node, and epidermis of the left shoulder. Esophagogastroscopy revealed an ulcerative and infiltrating type tumor in the middle thoracic esophagus. Based on histopathologic examination of the endoscopic biopsy and the resected cutaneous tumor, the patient was diagnosed as esophageal BSCC with cutaneous metastasis. The patient was treated with chemotherapy followed by chemoradiotherapy. The therapeutic effect was a complete response, which was sustained for 39 months.Clinical discussionReview of previous literature in the PubMed database revealed only been two case reports on cutaneous metastasis of BSCC. Advanced BSCC of the esophagus with distant metastasis has a poor prognosis. Therefore, in our case, future careful follow-up is required.ConclusionEsophageal BSCC with cutaneous metastasis can be successfully managed by multidisciplinary treatment, including local resection of the cutaneous metastasis, systemic chemotherapy, and chemoradiotherapy.     

Immunohistochemical expression of bcl-2 protein in squamous cell carcinoma and basaloid carcinoma of the esophagus

In the present study, the expression of bcl-2 protein in esophageal squamous cell carcinoma (SCC) and basaloid carcinoma (BC) was immunohistochemically examined, and its relation to tumor progression and postoperative survival was determined in SCC.A total of 42 SCC and 4 BC tumor samples were fixed with formalin, embedded in paraffin, and stained using monoclonal bcl-2 protein antibody, clone 124. Immunoreactivity was semiquantitatively scored, and the staining results were compared with the pathologic features and survival rates. The cytoplasm of basal cells from the normal esophageal epithelium was stained. In some well- and moderately differentiated SCCs, bcl-2 protein-positive reaction was observed in the peripheral part of the tumor cord, but in poorly differentiated SCC, the cells were weakly or hardly stained. However, in BC, the cells were strongly stained. The immunoreactivity was positive in 45.2% of the SCCs and all of the BCs. There were no significant differences in pathological features or patient survival between the bcl-2 protein-positive and protein-negative SCCs. In conclusion, the expression was not related to tumor progression and had no prognostic significance in SCC. Conversely, BC had strong immuno-histochemical expression, probably associated with the differentiation of carcinoma cells simulating the basal cells of the esophagus.     

Prognostic significance of perioperative blood transfusion in oral cavity squamous cell carcinoma

BACKGROUND: The influence of perioperative blood transfusion on survival in squamous cell carcinoma of the head and neck is still not convincingly determined. To investigate the effect of perioperative allogeneic transfusion on survival in stage II-IV squamous cell carcinoma of the oral cavity, we studied a consecutive series of 105 patients undergoing primary tumor resection and neck dissection. METHODS: Retrospective analyses were performed using Cox proportional hazards models with 16 variables. RESULTS: Perioperative red blood cell transfusion was required in 64 (61%) patients. Multivariate analysis demonstrated that the number of positive nodes and > or =3 units of red blood cell transfusion were an independent prognostic indicators. The calculated odds ratio for death after > or =3 units transfused was 5.79 (95% confidence interval, 1.56-21.53, p <.01). CONCLUSIONS: More than 3 units of allogeneic red blood cells transfused might shorten the survival of patients with oral cavity cancer.