桂竹糖芥强心苷G和哇巴因的强心效应和安全范围的比较

在猫和豚鼠戊巴比妥钠心衰模型上比较了桂竹糖芥强心苷Ｇ和哇巴因的强心作用和安全范围。桂竹糖芥强心苷Ｇ作用于衰竭心脏的最大有效量为（８８±１２）μｇ／ｋｇ（猫），（１３６±１ｇ）μｇ／ｋｇ（豚鼠），桂竹糖芥强心苷Ｇ安全范围大于哇巴因。     

HPLC法测定糖芥中强心苷含量

目的建立桂竹糖芥有效成分的含量测定方法。方法以地产的桂竹糖芥为分析对象，利用高效液相色谱法测定用不同方法提取的糖芥强心苷的含量。结果方法学考察表明，本研究建立的分析方法有比较好的重现性。结论该法简便，结果准确，可用于中药材的有效成分分析。     

糖芥苷的提取分离及其细胞毒性的考察

目的：考察糖芥苷的细胞毒性。方法：应用80％乙醇回流提取,正己烷萃取,MCI柱层析甲醇洗脱获得糖芥苷。采用体外培养的血管平滑肌细胞,通过检测细胞增殖、细胞内钙和c—myc基因的mRNA表达,考察糖芥苷细胞毒性,并与哇巴因、地高辛进行比较。结果：（1）糖芥苷浓度在10^-5mg／mL时具有促血管平滑肌细胞增殖作用,增殖率为33．3％;哇巴因和地高辛浓度分别在10^-14mg／mL和10^-9mg／mL时具有促血管平滑肌细胞增殖作用,最大增殖率分别为49．08％和51．05％。（2）糖芥苷浓度在10～mg／mL时可暂短升高细胞内钙;哇巴因浓度在10^-4mg／mL时可持久升高细胞内钙。（3）糖芥苷浓度在10—8mg／mL时对c—mycmRNA表达没有影响;哇巴因和地高辛浓度在10^-12mg／mL时可增加C—mycmRNA表达。结论：与哇巴因和地高辛相比,糖芥苷细胞毒性较小。     

HPLC法测定糖芥中强心苷含量

目的建立桂竹糖芥有效成分的含量测定方法。方法以地产的桂竹糖芥为分析对象,利用高效液相色谱法测定用不同方法提取的糖芥强心苷的含量。结果方法学考察表明,本研究建立的分析方法有比较好的重现性。结论该法简便,结果准确,可用于中药材的有效成分分析。     

桂竹糖芥中强心甙的种类及丰度研究

桂竹糖芥又名小花糖芥，是十字花科糖芥属植物。黑龙江省尚志、海林、伊春一带均有分布，资源较丰富。该植物含有多种强心甙，具有强心利尿作用。为发掘利用本省中草药资源和满足临床实践的需要，我们已将采自牡丹江北山一带的桂竹糖芥中起强心作用的化学成分强心总甙提取出来。化     

桂竹糖芥HPLC指纹图谱建立与分析

目的：建立桂竹糖芥的指纹图谱。方法：以黑龙江省牡丹江地产的桂竹糖芥为分析对象，选择适宜的条件，建立了桂竹糖芥的指纹图谱。结果：考察表明，本研究建立的分析方法有比较好的重现性。结论：指纹图谱分析方法可简便，快速的鉴别中药材。     

杜仲籽总苷抗炎镇痛作用研究

目的:研究杜仲籽总苷的抗炎、镇痛作用。方法:采用二甲苯所致小鼠耳肿胀和大鼠角叉菜胶足肿胀方法,研究杜仲籽总苷的抗炎作用;采用热板法、醋酸扭体法、光照甩尾法,研究杜仲籽总苷的镇痛作用。结果:杜仲籽总苷的110、220 mg/kg剂量都能明显减少二甲苯所致小鼠耳肿胀的体积,杜仲籽总苷的55、110、220 mg/kg剂量在给药后4 h左右明显减少大鼠角叉菜胶足肿胀率;杜仲籽总苷的220 mg/kg剂量能显著提高热板和光电引起小鼠的痛阈值,杜仲籽总苷的110、220 mg/kg剂量能显著地减少醋酸引起的扭体次数。结论:杜仲籽总苷具有较强的抗炎、镇痛作用。     

氧化苦参碱抗心律失常作用的实验研究

目的：观察氧化苦参碱的抗心律失常作用。方法：制备大鼠心律失常模型。结果：氧化苦参碱(Oxymatrine,OM)10mg／kg,20mg／kg能明显对抗乌头碱20ug／kg,Bacl2 2mg／ks和结扎左冠状动脉前降支诱发的大鼠室性心律失常。OM也能明显对抗Cacl2-Ach(Cacl2 0．6％ Ach 0．0025％)混合液10mg／kg诱发小鼠心房纤颤或扑动。大鼠心电图试验证明OM有负性频率作用,负性传导作用。这些作用可能是OM抗心律失常作用的药理学基础。结论：氧化苦参碱对大鼠有明显的抗心律失常作用。     

高含量银杏黄酮苷注射液的质量研究

目的 对高纯度银杏黄酮注射液(以下简称“EGB注射液”)进行质量分析及药物安全实验，并与德国生产的“金纳多”注射液(以下称“金纳多”)进行比较，找出质量的差异，为该制备方法的应用提供理论依据。方法 采用高效液相色谱分析仪(HPLC)，分析EGB注射液及“金纳多”有效成分及含量。结果 在银杏叶提取物均为17．5mg／5ml条件下，测得金纳多中银杏黄酮苷为4．2mg，占提取物的24％，水解苷元、槲皮素、山萘酚、异鼠李素含量分别为160．8μg／ml、144．87μg／ml、30．57μg／ml。总萜内酯含量为0．082mg／ml，萜内酯B约为零；该注射液中银杏黄酮苷为8．7mg，占提取物的49．8％，水解苷元、槲皮素、山萘酚、异鼠李素含量分别为440．5μg／ml、253．7μg／ml、69．42μg／ml，萜内酯总含量为0．1544mg／ml，萜内酯B为0．0532mg／ml。两项指标均高于“金纳多”。结论 EGB注射液中银杏黄酮含量成倍提高，萜内酯总含量较“金纳多”有所提高。     

甘草总黄酮抗心律失常作用研究

利用动物实验性心律失常病理模型，观察甘草总黄酮是否具有抗心律失常作用。结果表明，50～100mg／kg甘草总黄酮可延长乌头碱诱发的小鼠心律失常潜伏期、减少氯仿诱发的小鼠心室纤颤阳性率；25～50mg／kg甘草总黄酮可增加哇巴因诱发豚鼠出现定性早搏、室速、室额和心搏停止所用剂量，表明甘草总黄酮具有抗心律失常作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.